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Conserved domains on  [gi|67678038|gb|AAH97964|]
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Cholesterol 25-hydroxylase [Rattus norvegicus]

Protein Classification

sterol desaturase family protein( domain architecture ID 10513792)

sterol desaturase family protein is an oxidoreductase, similar to C-5 sterol desaturase, which catalyzes a dehydrogenation reaction to introduce a C5-6 double bond into substrates such as lathosterol and the B ring of ergosterol

EC:  1.-.-.-
Gene Ontology:  GO:0006629|GO:0016491

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
FA_hydroxylase pfam04116
Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol ...
 128-263 1.15e-26

Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol desaturases. Beta-carotene hydroxylase is involved in zeaxanthin synthesis by hydroxylating beta-carotene, but the enzyme may be involved in other pathways. This family includes C-5 sterol desaturase and C-4 sterol methyl oxidase. Members of this family are involved in cholesterol biosynthesis and biosynthesis a plant cuticular wax. These enzymes contain two copies of a HXHH motif which coordinate two irons at the catalytic centre. Members of this family are ER integral membrane proteins that share a novel mushroom-shaped fold consisting of four transmembrane (TM1-TM4) helices that anchor them to the membrane capped by a cytosolic domain containing the unique histidine- coordinating di metal centre.


:

Pssm-ID: 397991  Cd Length: 134  Bit Score: 101.74  E-value: 1.15e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 67678038   128 VLICLLLFDTEIFAWHLLHHKVPWLYRTFHKVHHQNSSSFALATQYMSVWELLSLTFFDVLNVAMLQCHPLTILVFHVVN 207
Cdd:pfam04116   1 VLLGLLLFDFLFYWVHRLLHRLPWLWRRFHKVHHSSEAPNALTALRFHPLEALLFALLVLLPLLLLGLPLLAFLLGLLLG 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 67678038   208 IWLSVEDHSGYDFPwstHRLVPFGWYGGVAHHDLHHSQFN-CNFAPYFTHWDKMLGT 263
Cdd:pfam04116  81 TLWYLFIHSGLLFP---LPWLLKRLLGTPRFHRLHHSKNEeYNFGVTFPLWDRLFGT 134
 
Name Accession Description Interval E-value
FA_hydroxylase pfam04116
Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol ...
 128-263 1.15e-26

Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol desaturases. Beta-carotene hydroxylase is involved in zeaxanthin synthesis by hydroxylating beta-carotene, but the enzyme may be involved in other pathways. This family includes C-5 sterol desaturase and C-4 sterol methyl oxidase. Members of this family are involved in cholesterol biosynthesis and biosynthesis a plant cuticular wax. These enzymes contain two copies of a HXHH motif which coordinate two irons at the catalytic centre. Members of this family are ER integral membrane proteins that share a novel mushroom-shaped fold consisting of four transmembrane (TM1-TM4) helices that anchor them to the membrane capped by a cytosolic domain containing the unique histidine- coordinating di metal centre.


Pssm-ID: 397991  Cd Length: 134  Bit Score: 101.74  E-value: 1.15e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 67678038   128 VLICLLLFDTEIFAWHLLHHKVPWLYRTFHKVHHQNSSSFALATQYMSVWELLSLTFFDVLNVAMLQCHPLTILVFHVVN 207
Cdd:pfam04116   1 VLLGLLLFDFLFYWVHRLLHRLPWLWRRFHKVHHSSEAPNALTALRFHPLEALLFALLVLLPLLLLGLPLLAFLLGLLLG 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 67678038   208 IWLSVEDHSGYDFPwstHRLVPFGWYGGVAHHDLHHSQFN-CNFAPYFTHWDKMLGT 263
Cdd:pfam04116  81 TLWYLFIHSGLLFP---LPWLLKRLLGTPRFHRLHHSKNEeYNFGVTFPLWDRLFGT 134
ERG3 COG3000
Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid ...
 128-269 1.92e-18

Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid transport and metabolism];


Pssm-ID: 442238  Cd Length: 158  Bit Score: 80.36  E-value: 1.92e-18
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 67678038 128 VLICLLLFDTEIFAWHLLHHKVPWLYRtFHKVHHQNSSSFALATQYMSVWELLSLTFFDVLNVAMLQCHPLTILVFHVVN 207
Cdd:COG3000   2 FLLAFLLLDFVYYWLHRLSHRVPLLWR-FHAVHHSSERMNLLTALRFHPLEILLSALLFLLPLALLGFPPEAVLLYLALN 80

                        90       100       110       120       130       140       150
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 67678038 208 IWLSVEDHSGYDFPWSthrlvpfGWYGGV----AHHDLHHSQ----FNCNFAPYFTHWDKMLGTLRCAPH 269
Cdd:COG3000  81 LLYQFFNHSNIRLPLD-------GPLEYVfvtpSHHRVHHSRnpeyLDKNYGGILSIWDRLFGTYRPEER 143
 
Name Accession Description Interval E-value
FA_hydroxylase pfam04116
Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol ...
 128-263 1.15e-26

Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol desaturases. Beta-carotene hydroxylase is involved in zeaxanthin synthesis by hydroxylating beta-carotene, but the enzyme may be involved in other pathways. This family includes C-5 sterol desaturase and C-4 sterol methyl oxidase. Members of this family are involved in cholesterol biosynthesis and biosynthesis a plant cuticular wax. These enzymes contain two copies of a HXHH motif which coordinate two irons at the catalytic centre. Members of this family are ER integral membrane proteins that share a novel mushroom-shaped fold consisting of four transmembrane (TM1-TM4) helices that anchor them to the membrane capped by a cytosolic domain containing the unique histidine- coordinating di metal centre.


Pssm-ID: 397991  Cd Length: 134  Bit Score: 101.74  E-value: 1.15e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 67678038   128 VLICLLLFDTEIFAWHLLHHKVPWLYRTFHKVHHQNSSSFALATQYMSVWELLSLTFFDVLNVAMLQCHPLTILVFHVVN 207
Cdd:pfam04116   1 VLLGLLLFDFLFYWVHRLLHRLPWLWRRFHKVHHSSEAPNALTALRFHPLEALLFALLVLLPLLLLGLPLLAFLLGLLLG 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 67678038   208 IWLSVEDHSGYDFPwstHRLVPFGWYGGVAHHDLHHSQFN-CNFAPYFTHWDKMLGT 263
Cdd:pfam04116  81 TLWYLFIHSGLLFP---LPWLLKRLLGTPRFHRLHHSKNEeYNFGVTFPLWDRLFGT 134
ERG3 COG3000
Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid ...
 128-269 1.92e-18

Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid transport and metabolism];


Pssm-ID: 442238  Cd Length: 158  Bit Score: 80.36  E-value: 1.92e-18
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 67678038 128 VLICLLLFDTEIFAWHLLHHKVPWLYRtFHKVHHQNSSSFALATQYMSVWELLSLTFFDVLNVAMLQCHPLTILVFHVVN 207
Cdd:COG3000   2 FLLAFLLLDFVYYWLHRLSHRVPLLWR-FHAVHHSSERMNLLTALRFHPLEILLSALLFLLPLALLGFPPEAVLLYLALN 80

                        90       100       110       120       130       140       150
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 67678038 208 IWLSVEDHSGYDFPWSthrlvpfGWYGGV----AHHDLHHSQ----FNCNFAPYFTHWDKMLGTLRCAPH 269
Cdd:COG3000  81 LLYQFFNHSNIRLPLD-------GPLEYVfvtpSHHRVHHSRnpeyLDKNYGGILSIWDRLFGTYRPEER 143
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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