BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain superfamily; Proteins in this superfamily are characterized by the presence of a common protein-protein interaction motif of about 100 amino acids, known as the BTB/POZ domain. Members include transcription factors, oncogenic proteins, ion channel proteins, and potassium channel tetramerization domain (KCTD) proteins. They have been identified in poxviruses and many eukaryotes, and have diverse functions, such as transcriptional regulation, chromatin remodeling, protein degradation and cytoskeletal regulation. Many BTB/POZ proteins contain one or two additional domains, such as kelch repeats, zinc-finger domains, FYVE (Fab1, YOTB, Vac1, and EEA1) fingers, or ankyrin repeats, among others. These special additional domains or interaction partners provide unique characteristics and functions to BTB/POZ proteins. In ion channel proteins and KCTD proteins, the BTB/POZ domain is also called the tetramerization (T1) domain.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157816985  13 GSDRLVQLAARLRPA--LCDTLITVGGQEFLAHSLVLAGASPRLG------CRGRWALVEGISPSTFAQLLTFVYGESVE 84
Cdd:cd18218    1 HSDRLLHLAARLRPAgaLCDTLITVGSQEFPAHSLVLAGASQQLGrqltnpNPGQWCLGEGISPSTFKQLLDFVYGESVE 80

                         90       100
                 ....*....|....*....|....*...
gi 157816985  85 LQPGELGNLEEAARALGVQALEEACKRA 112
Cdd:cd18218   81 VQPGELRPLLEAARALEVESLEEACWRA 108

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in zinc finger and BTB domain-containing protein 32 (ZBTB32); ZBTB32 is also called FANCC-interacting protein, fanconi anemia zinc finger protein (FAZF), testis zinc finger protein (TZFP), or zinc finger protein 538 (ZNF538). It is a DNA-binding transcription factor that binds to the 5'-TGTACAGTGT-3' core sequence. It acts as a transcription suppressor that controls T cell-mediated autoimmunity. ZBTB32 is essential for down-regulation of GATA3 via ZPO2; this promotes aggressive breast cancer development. It contains a BTB/POZ domain, a common protein-protein interaction motif of about 100 amino acids.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157816985  13 GSDRLVQLAARLRPA--LCDTLITVGGQEFLAHSLVLAGASPRLG------CRGRWALVEGISPSTFAQLLTFVYGESVE 84
Cdd:cd18218    1 HSDRLLHLAARLRPAgaLCDTLITVGSQEFPAHSLVLAGASQQLGrqltnpNPGQWCLGEGISPSTFKQLLDFVYGESVE 80

                         90       100
                 ....*....|....*....|....*...
gi 157816985  85 LQPGELGNLEEAARALGVQALEEACKRA 112
Cdd:cd18218   81 VQPGELRPLLEAARALEVESLEEACWRA 108

Broad-Complex, Tramtrack and Bric a brac; Domain in Broad-Complex, Tramtrack and Bric a brac. Also known as POZ (poxvirus and zinc finger) domain. Known to be a protein-protein interaction motif found at the N-termini of several C2H2-type transcription factors as well as Shaw-type potassium channels. Known structure reveals a tightly intertwined dimer formed via interactions between N-terminal strand and helix structures. However in a subset of BTB/POZ domains, these two secondary structures appear to be missing. Be aware SMART predicts BTB/POZ domains without the beta1- and alpha1-secondary structures.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157816985    30 DTLITVGGQEFLAHSLVLAGASP-----RLGCRGRWALVE----GISPSTFAQLLTFVYGESVELQPGELGNLEEAARAL 100
Cdd:smart00225   1 DVTLVVGGKKFHAHKAVLAAHSPyfkalFSSDFKESDKSEiyldDVSPEDFRALLNFLYTGKLDLPEENVEELLELADYL 80

                           90
                   ....*....|..
gi 157816985   101 GVQALEEACKRA 112
Cdd:smart00225  81 QIPGLVELCEEF 92

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.

                          10        20
                  ....*....|....*....|...
gi 157816985  368 YSCSVCGKRFSLKHQMETHYRVH 390
Cdd:pfam00096   1 YKCPDCGKSFSRKSNLKRHLRTH 23

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in zinc finger and BTB domain-containing protein 32 (ZBTB32); ZBTB32 is also called FANCC-interacting protein, fanconi anemia zinc finger protein (FAZF), testis zinc finger protein (TZFP), or zinc finger protein 538 (ZNF538). It is a DNA-binding transcription factor that binds to the 5'-TGTACAGTGT-3' core sequence. It acts as a transcription suppressor that controls T cell-mediated autoimmunity. ZBTB32 is essential for down-regulation of GATA3 via ZPO2; this promotes aggressive breast cancer development. It contains a BTB/POZ domain, a common protein-protein interaction motif of about 100 amino acids.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157816985  13 GSDRLVQLAARLRPA--LCDTLITVGGQEFLAHSLVLAGASPRLG------CRGRWALVEGISPSTFAQLLTFVYGESVE 84
Cdd:cd18218    1 HSDRLLHLAARLRPAgaLCDTLITVGSQEFPAHSLVLAGASQQLGrqltnpNPGQWCLGEGISPSTFKQLLDFVYGESVE 80

                         90       100
                 ....*....|....*....|....*...
gi 157816985  85 LQPGELGNLEEAARALGVQALEEACKRA 112
Cdd:cd18218   81 VQPGELRPLLEAARALEVESLEEACWRA 108

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in Influenza virus NS1A-binding protein (NS1-BP); NS1-BP is also called NS1-binding protein, aryl hydrocarbon receptor-associated protein 3 (ARA3), or IVNS1ABP. It is a novel protein that interacts with the influenza A virus nonstructural NS1 protein, which is relocalized in the nuclei of infected cells. It plays a role in cell division and in the dynamic organization of the actin skeleton as a stabilizer of actin filaments by association with F-actin through its kelch repeats. It also interacts with alpha-enolase/MBP-1 and is involved in c-Myc gene transcriptional control. NS1-BP contains BTB and BACK domains at the N-terminal region and kelch repeats at the C-terminal region. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157816985  28 LCDTLITVGGQEFLAHSLVLAGASPRL---------GCRGRWALVEGISPSTFAQLLTFVYGESVELQPGELGNLEEAAR 98
Cdd:cd18306   18 FCDVILQVGGHEIPAHRAVLACASPYLfelfssdsdGESILTVKLDGLDPDAVEVLVNYAYTSRLEVPADLVKSVYSAAK 97

                         90
                 ....*....|.
gi 157816985  99 ALGVQALEEAC 109
Cdd:cd18306   98 KLKMDRVKKAC 108

BTB/POZ domain; The BTB (for BR-C, ttk and bab) or POZ (for Pox virus and Zinc finger) domain is present near the N-terminus of a fraction of zinc finger (pfam00096) proteins and in proteins that contain the pfam01344 motif such as Kelch and a family of pox virus proteins. The BTB/POZ domain mediates homomeric dimerization and in some instances heteromeric dimerization. The structure of the dimerized PLZF BTB/POZ domain has been solved and consists of a tightly intertwined homodimer. The central scaffolding of the protein is made up of a cluster of alpha-helices flanked by short beta-sheets at both the top and bottom of the molecule. POZ domains from several zinc finger proteins have been shown to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes including N-CoR and SMRT. The POZ or BTB domain is also known as BR-C/Ttk or ZiN.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157816985   23 RLRPALCDTLITVGGQEFLAHSLVLAGASPRL---------GCRGRWALVEGISPSTFAQLLTFVYGESVeLQPGELGNL 93
Cdd:pfam00651   5 REQGELCDVTLVVGDKEFRAHKAVLAACSPYFkalfsgqesESSVSEITLDDVSPEDFEALLEFMYTGKL-ISEENVDDL 83

                          90
                  ....*....|....*.
gi 157816985   94 EEAARALGVQALEEAC 109
Cdd:pfam00651  84 LAAADKLQIPSLVDKC 99

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in Kelch-like protein 12 (KLHL12); KLHL12, also called CUL3-interacting protein 1 (C3IP1) or DKIR homolog, is a substrate-specific adaptor of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a negative regulator of the Wnt signaling pathway and ER-Golgi transport. It contains a BTB domain and kelch repeats, characteristics of a kelch family protein. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157816985  23 RLRPA--LCDTLITVGGQEFLAHSLVLAGASPRLGCRGRWALVE---------GISPSTFAQLLTFVYGESVELQPGELG 91
Cdd:cd18242   14 SLRKSntLCDVTLRVEGKEFPAHRIVLAACSDYFCAMFTSEMSEkgksevelqGLTASTMEILLDFVYTETVHVTVENVQ 93

                         90
                 ....*....|....*...
gi 157816985  92 NLEEAARALGVQALEEAC 109
Cdd:cd18242   94 ELLPAACLLQLKGVKQAC 111

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in zinc finger and BTB domain-containing protein 16 (ZBTB16); ZBTB16 is also called promyelocytic leukemia zinc finger protein, zinc finger protein 145, or zinc finger protein PLZF. It is a DNA-binding transcription factor essential for undifferentiated cell maintenance. ZBTB16 also acts as a downstream transcriptional regulator of Osterix and can be useful as a late marker of osteoblastic differentiation. It contains a BTB/POZ domain, a common protein-protein interaction motif of about 100 amino acids.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157816985  23 RLRPALCDTLITVGGQEFLAHSLVLAGASPRLGC----RGRWALVEGISPSTFAQLLTFVYGESVELQPGELGNLEEAAR 98
Cdd:cd18205   13 RLAGTLCDVVIMVDSQEFHAHRTVLACTSKMFEIlfhrNSQHYTLDFLSPKTFQQILEYAYTATLQAKVEDLDDLLYAAE 92

                         90
                 ....*....|.
gi 157816985  99 ALGVQALEEAC 109
Cdd:cd18205   93 ILEIEYLEEQC 103

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in zinc finger and BTB domain-containing protein 37 (ZBTB37); ZBTB37 may be involved in transcriptional regulation. It is differentially expressed in aryl hydrocarbon receptor (AhR)-KO mice compared with WT mice, and may potentially contribute to the aging phenotype of AhR-KO mice. It contains a BTB/POZ domain, a common protein-protein interaction motif of about 100 amino acids.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157816985  23 RLRPALCDTLITVGGQEFLAHSLVLAGASPRLgcRGRWALVE----GIS----PSTFAQLLTFVYGESVELQPGELGNLE 94
Cdd:cd18222   20 RMQGRLCDIVVNVQGQSFRAHKVVLAASSPYF--RDHMSLNEmstvSISviknPTVFEQLLSFCYTGRICLQLADIISYL 97

                         90
                 ....*....|....*..
gi 157816985  95 EAARALGVQALEEACKR 111
Cdd:cd18222   98 TAASFLQMQHIIDKCTQ 114

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in BTB and CNC homolog (BACH) proteins; This subfamily includes BACH1 (also called BTB-basic leucine zipper transcription factor 1), BACH2 (also called BTB-basic leucine zipper transcription factor 2), and similar proteins. They belong to the cap 'n' collar (CNC) and basic leucine zipper (bZIP) factor family. BACH1 is a heme-responsive transcriptional repressor of heme oxygenase (HO)-1. It represses genes involved in heme metabolism and oxidative stress response. BACH2 is a lymphoid-specific transcription factor with a prominent role in B-cell development. It is transcriptionally regulated by the BCR/ABL oncogene. It represses the anti-apoptotic factor heme oxygenase-1 (HO-1). Subfamily members contain a BTB domain and a basic leucine zipper (bZIP) domain. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157816985  28 LCDTLITVGGQEFLAHSLVLAGASPRLGCRGRW--------ALVEGISPSTFAQLLTFVYGESVELQPGELGNLEEAARA 99
Cdd:cd18955    1 LCDVTIVVEDQRFRAHRAVLAACSDYFLSRVVGqtdgdlviTLPEEVTVKGFEPLLQFAYTAKLLLSKENVLEVCRCAEF 80

                         90
                 ....*....|
gi 157816985 100 LGVQALEEAC 109
Cdd:cd18955   81 LGVHNIEKAC 90

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in zinc finger and BTB domain-containing protein 41 (ZBTB41); ZBTB41, also called FRBZ1, may be involved in transcriptional regulation. Its specific function is as yet unknown. It contains a BTB/POZ domain, a common protein-protein interaction motif of about 100 amino acids.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157816985  15 DRLVQlaarlrPALCDTLITVGGQEFLAHSLVLA-GASPRLGCRGR-----WALVEGISPSTFAQLLTFVYGESVELQPG 88
Cdd:cd18226   15 DRQKQ------SSFCDLVITVEGKEFSAHKVVVAvGSSYFHACLSKnpntdAVTLDHVSYSVFQHLLEFLYTSEFFVYEN 88

                         90
                 ....*....|....
gi 157816985  89 ELGNLEEAARALGV 102
Cdd:cd18226   89 EIPLVLEAAKFLDI 102

first BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in Rho-related BTB domain-containing proteins (RhoBTB); RhoBTB proteins constitute a subfamily of atypical members within the Rho family of small guanosine triphosphatases (GTPases), which is characterized by containing a GTPase domain (in most cases, non-functional) followed by a proline-rich region, tandem BTB domains, and a conserved C-terminal region. In vertebrates, the RhoBTB subfamily consists of 3 isoforms: RhoBTB1, RhoBTB2, and RhoBTB3. Orthologs are present in several other eukaryotes, such as Drosophila and Dictyostelium, but have been lost in plants and fungi. This model corresponds to the first BTB domain. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157816985  31 TLITVGGQEFLAHSLVLAGASPR----LGCRGRWALVEGISPSTFAQLLTFVYGESVELQPGELGNLEEAARALGVQALE 106
Cdd:cd18299   16 VFILQGGVRIFAHRIVLAAASSVfadlFLMMTVVTLDSDITPEAFRRVLEFLYTGVLDENEDDLKELKDAAELLELFDLV 95

                 ....*
gi 157816985 107 EACKR 111
Cdd:cd18299   96 MMCTN 100

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in Kelch-like protein 10 (KLHL10); KLHL10 is a substrate-specific adaptor of a CUL3-based E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins specifically in the testis during spermatogenesis. Haploinsufficiency of Klhl10 causes infertility in male mice. KLHL10 contains a BTB domain and kelch repeats, characteristics of a kelch family protein. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157816985  21 AARLRPALCDTLITVGGQEFLAHSLVLAGASP--RLGCRGRWA-------LVEGISPSTFAQLLTFVYGESVELQPGELG 91
Cdd:cd18240   10 ELRLEGKLCDVVIRVNGVEFPAHRNILCACSPyfRALFTNGWNetekkvyKIPGVSPEMMELIIEYAYTRTVPITAENVE 89

                         90
                 ....*....|....*...
gi 157816985  92 NLEEAARALGVQALEEAC 109
Cdd:cd18240   90 ELLPAADQFNIMGIVKAC 107

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in zinc finger and BTB domain-containing protein 7 (ZBTB7); There are three ZBTB7 isoforms: ZBTB7A, ZBTB7B, and ZBTB7C. ZBTB7A is a transcription repressor of key glycolytic genes, including GLUT3, PFKP, and PKM, and its downregulation in human cancer contributes to tumor metabolism. ZBTB7B is a transcriptional regulator of extracellular matrix gene expression. ZBTB7C is a transcriptional repressor with a pro-oncogenic role that relies upon binding to p53 and inhibition of its transactivation function. ZBTB7 isoforms contain a BTB/POZ domain, a common protein-protein interaction motif of about 100 amino acids.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157816985  28 LCDTLITVGGQEFLAHSLVLAGAS---PRLGCRGrwALVEG--------ISPSTFAQLLTFVYGESVELQPGELGNLEEA 96
Cdd:cd18198   25 LCDVVILVQGREYRTHRSVLAACSqyfKKLFTSG--AVVDQqnvyeidfVSPEALTALLEFAYTATLTVSTANVGDILSA 102

                         90
                 ....*....|...
gi 157816985  97 ARALGVQALEEAC 109
Cdd:cd18198  103 ARLLEIPCVSHVC 115

second BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in BTB/POZ domain-containing protein 8 (BTBD8); BTBD8 is a BTB-domain-containing Kelch-like protein that may play a role in developmental processes. It may also act as a protein-protein adaptor in a transcription complex and thus be involved in brain development. BTBD8 contains two BTB domains. This model corresponds to the second domain. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157816985  30 DTLITVGGQEFLAHSLVLAGASPRLGC--RGRWAL-------VEGISPSTFAQLLTFVYGESVELQPGE-LGNLEEAARA 99
Cdd:cd18286   19 DITIKVDGKTFKAHRCILCARSSYFAAmlSGSWAEsnsseitLTGVSHAAVSFVLLFIYGGVLDLPDDVnLGELLSLADM 98

                 ....*....
gi 157816985 100 LGVQALEEA 108
Cdd:cd18286   99 YGLDGLKDV 107

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in Kelch-like ECH-associated protein 1 (KEAP1); KEAP1, also called cytosolic inhibitor of Nrf2 (INrf2) or Kelch-like protein 19 (KLHL19), is a redox-regulated substrate adaptor protein for a Cullin3-dependent ubiquitin ligase complex that targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. It contains a BTB domain and kelch repeats, characteristics of a kelch family protein. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157816985  23 RLRPALCDTLITVGGQ----EFLAHSLVLAGASPRL------GCRGRWALV---EGISPSTFAQLLTFVY 79
Cdd:cd18248   16 RLSQQLCDVTLKVKYQdpkeEFMAHKVVLASSSPYFkamftsGLRECGMEVvpiEGVHPCVMSRLIEFAY 85

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in Kelch-like protein 17 (KLHL17); KLHL17, also called actinfilin, is a substrate-recognition component of some cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes. It acts as a Cullin 3 (Cul3) substrate adaptor that links GLUR6 to the E3 ubiquitin-ligase complex, and mediates the ubiquitination and subsequent degradation of GLUR6. It may play a role in actin-based neuronal function. It contains a BTB domain and kelch repeats, characteristics of a kelch family protein. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157816985  23 RLRPALCDTLITVGGQEFLAHSLVLAGASPR---------LGCRGRWALVEGISPSTFAQLLTFVYGESVELQPGELGNL 93
Cdd:cd18246   34 RQRGLLCDIVLHVGTKEIKAHKVVLASCSPYfhamftnemSESRQTHVTLHDIDPQALEQLVQYAYTAEIVVGEGNVQTL 113

                         90
                 ....*....|....*.
gi 157816985  94 EEAARALGVQALEEAC 109
Cdd:cd18246  114 LPAASLLQLNGVRDAC 129

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in B-cell lymphoma 6 proteins, BCL-6 and BCL-6B; This family includes B-cell lymphoma 6 proteins, BCL-6 and BCL-6B. BCL-6 is a transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation, which have different mechanisms of action specific to the lineage and biological functions. BCL-6B is a sequence-specific transcriptional repressor in association with BCL-6. It may function in a narrow stage or be related to some events in the early B-cell development. Family members contain a BTB/POZ domain, a common protein-protein interaction motif of about 100 amino acids.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157816985  14 SDRLVQLAA-RLRPALCDTLITVGGQEFLAHSLVLAGASPRLGCRGRWALVEGIS---------PSTFAQLLTFVYGESV 83
Cdd:cd18215    4 SDVLLNLNRlRSRDILTDVVLVVGREQFRAHKTVLMACSGLFYSIFTDQLKNNVSvinldpeiePEGFRILLDFMYTSRL 83

                         90       100
                 ....*....|....*....|....*...
gi 157816985  84 ELQPGELGNLEEAARALGVQALEEACKR 111
Cdd:cd18215   84 NLREGNIMAVMATAMYLQMEHVVDTCRK 111

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in B-cell CLL/lymphoma 6 member B protein (BCL6B); BCL6B is also called Bcl6-associated zinc finger protein, zinc finger protein 62, or zinc finger and BTB domain-containing protein 28 (ZBTB28). It is a sequence-specific transcriptional repressor in association with BCL-6. It may function in a narrow stage or be related to some events in the early B-cell development. BCL6B plays an important role as a potential tumor suppressor in gastric cancer; it is found preferentially methylated in gastric cancer. It also inhibits both colorectal cancer growth and hepatocellular carcinoma metastases. BCL6B contains a BTB/POZ domain, a common protein-protein interaction motif of about 100 amino acids.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157816985  12 YGSDRLVQL-AARLRPALCDTLITVGGQEFLAHSLVLAGAS--------PRLGCRGR-WALVEGISPSTFAQLLTFVYGE 81
Cdd:cd18332    2 HSSDVLLNLnELRKRDILTDVTLLVGGTPLRAHKAVLIACSgffysifsGRVGEGVSvLTLPSGLEARGFQLLLDFMYTS 81

                         90       100       110
                 ....*....|....*....|....*....|
gi 157816985  82 SVELQPGELGNLEEAARALGVQALEEACKR 111
Cdd:cd18332   82 RLPLTPRTVPAVLAAATYLQMEHVVETCRR 111

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in Kelch-like protein 35 (KLHL35); KLHL35 contains a BTB domain and kelch repeats, characteristics of a kelch family protein. Its function remains unclear. Significant differences in DNA methylation of the KLHL35 gene in abdominal aortic aneurysm (AAA) patients compared to non-AAA controls suggest a potential role in AAA pathology. Hypermethylation of the KLHL35 gene has also been associated with the development of hepatocellular carcinoma. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157816985  28 LCDTLITVGGQEFLAHSLVLAGASP---------RLGCRGRWALVEGISPSTFAQLLTFVYGESVELQPGELGNLEEAAR 98
Cdd:cd18265   23 FTDVVLLVDGKDFPCHRATLSANSAyframfgghLKESRQAVVEIQKVSAAAMEVLLDYMYGGGLRIQEDNVESVLEASD 102

                         90
                 ....*....|...
gi 157816985  99 ALGVQALEEACKR 111
Cdd:cd18265  103 LLQVSKLREACVR 115

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in myoneurin; Myoneurin, also called zinc finger and BTB domain-containing protein 31 (ZBTB31), is a novel member of the BTB/POZ-zinc finger family highly expressed in the neuromuscular system and is associated with neuromuscular junctions during the late embryonic period. It may function as a synaptic gene regulator. Myoneurin contains a BTB/POZ domain, a common protein-protein interaction motif of about 100 amino acids.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157816985  28 LCDTLITVGGQEFLAHSLVLAGASPRLGCRGRWAL-------VEGISPSTFAQLLTFVYGESVELQPGELGNLEEAARAL 100
Cdd:cd18217   18 LCDCTIVIGQFQFKAHRNVLAAFSEYFGAQYKDTEdnivfldQSQVSADGFQKLLEFIYTGNLNLDSWNVAEIHQAASYL 97

                         90
                 ....*....|
gi 157816985 101 GVQALEEACK 110
Cdd:cd18217   98 GMEEVVAKCK 107

BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ (poxvirus and zinc finger) domain found in Dictyostelium discoideum trishanku and similar proteins; Trishanku is a novel regulator required for normal morphogenesis and cell-type stability in Dictyostelium discoideum. It contains a BTB domain. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157816985  30 DTLITVGGQEFLAHSLVLAGASP---------RLGCRGRWALVEGISPSTFAQLLTFVYGESVELQPGELGNLEEAARAL 100
Cdd:cd18314    8 DVVLCVGDRKFFAHRIVLCARSPvfrsmltgsMIESNLKEVTLEDVEPEIFETVLKYMYTGQVTLSEENVLDLLMLASKY 87

                 ....*....
gi 157816985 101 GVQALEEAC 109
Cdd:cd18314   88 QVPDLEKLC 96

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in Kelch-like protein 30 (KLHL30); KLHL30 contains a BTB domain and kelch repeats, characteristics of a kelch family protein. Its function remains unclear. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157816985  23 RLRPALCDTLITVGGQEFLAHSLVLAGASPRLGCRGRWALVEGIS---------PSTFAQLLTFVYGESVELQPGELGNL 93
Cdd:cd18259   27 RSNPKLSDVTLLVGGREFPCHRSVLALCSHYFNAMFTGDFVESISarveikdvdPAVMESLIDFAYTGKLTINQGNVEAL 106

                         90
                 ....*....|....*...
gi 157816985  94 EEAARALGVQALEEACKR 111
Cdd:cd18259  107 IRTSNRLQFPTVRKVCSR 124

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in Kelch-like protein 1 (KLHL1); KLHL1 is a neuronal actin-binding protein that modulates voltage-gated CaV2.1 (P/Q-type) and CaV3.2 (alpha1H T-type) calcium channels. It may play a role in organizing the actin cytoskeleton the brain cells. KLHL1 contains a BTB domain and kelch repeat domains, characteristics of a kelch family protein. The BTB/POZ domain is a common protein-protein interaction motif of about 100 amino acids.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157816985  28 LCDTLITVGGQEFLAHSLVLAGASPRLG-------CRGRWALV--EGISPSTFAQLLTFVYGESVELQPGELGNLEEAAR 98
Cdd:cd18335   19 LCDVILIAGNRKIPAHRLVLSAVSDYFAamftsdvCEAKQEEIkmEGIDPNALWDLVQFAYTGCLELKEDTIENLLAAAC 98

                         90
                 ....*....|.
gi 157816985  99 ALGVQALEEAC 109
Cdd:cd18335   99 LLQLSQVVEVC 109

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in zinc finger and BTB domain-containing protein 3 (ZBTB3); ZBTB3 is a transcription factor essential for cancer cell growth via the regulation of the reactive oxygen species (ROS) detoxification pathway. ZBTB3 contains a BTB/POZ domain, a common protein-protein interaction motif of about 100 amino acids.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157816985  11 PYGSDRLVQ--LAARLRPALCDTLITVGGQEFLAHSLVLAGASP--RLGCRGRWA---LV----EGISPSTFAQLLTFVY 79
Cdd:cd18323    4 PDHSRHLLQslREQRHQGFLCDCTVLVGSTQFLAHRAVLASCSPffHMFYKEQLDkrdLVcinnEIVTAPAFSLLLEFMY 83

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|..
gi 157816985  80 GESVELQPGELGNLEEAARALGVQALEEACK-RAQAAKNHEA 120
Cdd:cd18323   84 EGRLSFNDTPVEDVLAAASYLHMNDIVKVCKkRLQARALAEA 125

FOG: Zn-finger [General function prediction only];

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157816985 272 SHSTPITAAWQVWPQDQRIPLtlNHCKGLWSENQlaSSSPTPGSFPQGTEKLSPWEAEESGQgyTGTLATCVSQERRLNC 351
Cdd:COG5048  191 NVSTSIPSSSENSPLSSSYSI--PSSSSDQNLEN--SSSSLPLTTNSQLSPKSLLSQSPSSL--SSSDSSSSASESPRSS 264

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157816985 352 PSHQHPPLPSP---------ARSRPYSCSVCGKRFSLKHQMETHYR--VHTGE--KPFSC--SLCPQRSRDFSAMTKHLR 416
Cdd:COG5048  265 LPTASSQSSSPnesdsssekGFSLPIKSKQCNISFSRSSPLTRHLRsvNHSGEslKPFSCpySLCGKLFSRNDALKRHIL 344

                 ..
gi 157816985 417 TH 418
Cdd:COG5048  345 LH 346

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in zinc finger and BTB domain-containing protein 43 (ZBTB43); ZBTB43, also called zinc finger and BTB domain-containing protein 22B (ZBTB22b), zinc finger protein 297B (ZNF297B), or ZnF-x, may be involved in transcriptional regulation. It interacts with BDP1, a subunit of transcription factor IIIB (TFIIIB). Since BDP1 is essential in Pol III transcription, ZBTB43 may also regulate these transcriptional pathways. It contains a BTB/POZ domain, a common protein-protein interaction motif of about 100 amino acids.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157816985  23 RLRPALCDTLITVGGQEFLAHSLVLAGASPR------LGCRGRWALVEGISPSTFAQLLTFVYGESVELQPGELGNLEEA 96
Cdd:cd18227   20 RQQGQLCDISVVVQGHLFRAHKAVLAASSPYfcdqvlLKNSRRIVLPDVMNPRVFENILLSCYTGRLVLPAPEIVSYLTA 99

                         90
                 ....*....|...
gi 157816985  97 ARALGVQALEEAC 109
Cdd:cd18227  100 ASFLQMWHVVDKC 112

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.

                          10        20
                  ....*....|....*....|...
gi 157816985  368 YSCSVCGKRFSLKHQMETHYRVH 390
Cdd:pfam00096   1 YKCPDCGKSFSRKSNLKRHLRTH 23

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in zinc finger and BTB domain-containing protein 9 (ZBTB9); ZBTB9 may be involved in transcriptional regulation. It contains a BTB/POZ domain, a common protein-protein interaction motif of about 100 amino acids.

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 157816985  23 RLRPALCDTLITVGGQEFLAHSLVLAGASPR------LGCRGRWALVEGISPSTFAQLLTFVY 79
Cdd:cd18200   13 RLEGKFCDLSVHVQGRVFRAHKAVLAASSPYfhdkllLNDTNCLVLPSVIEAAAFENLLQLIY 75

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in zinc finger and BTB domain-containing proteins, ZBTB3, ZBTB18, ZBTB42 and similar proteins; The family includes zinc finger and BTB domain-containing proteins, ZBTB3, ZBTB18 and ZBTB42. ZBTB3 is a transcription factor essential for cancer cell growth via the regulation of the reactive oxygen species (ROS) detoxification pathway. ZBTB18 is a sequence-specific transrepressor associated with heterochromatin. ZBTB42 is a transcriptional repressor that specifically binds DNA and probably acts by recruiting chromatin remodeling multiprotein complexes. Members of this family contain a BTB/POZ domain, a common protein-protein interaction motif of about 100 amino acids.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157816985  11 PYGSDRLVQL--AARLRPALCDTLITVGGQEFLAHSLVLAGASP--------RLGCRGRWAL-VEGISPSTFAQLLTFVY 79
Cdd:cd18194    4 PEHSRQLLQClrEQRSQGFLCDCTVLVGSAQFPAHRAVLASCSMyfhlfyrdRLDKRDLVRLnSDIVTAPAFGLLLDFMY 83

                         90       100       110
                 ....*....|....*....|....*....|....*..
gi 157816985  80 GESVELQPGELGNLEEAARALGVQALEEACKRAQAAK 116
Cdd:cd18194   84 EGRLALRDLPVEDVLAAASYLHMYDIVKVCKGRLQEK 120

BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in zinc finger and BTB domain-containing protein 5 (ZBTB5); ZBTB5 is a POZ domain Kruppel-like zinc finger (POK) family transcription repressor of cell cycle arrest gene p21 and a potential proto-oncogene stimulating cell proliferation. ZBTB5 contains a BTB/POZ domain, a common protein-protein interaction motif of about 100 amino acids.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157816985  23 RLRPALCDTLITVGGQEFLAHSLVLAGASPRLgcRGRWALVEG-------------ISPSTFAQLLTFVYGESVELQPGE 89
Cdd:cd18196   16 RLHGQLCDCVIVVGNRHFKAHRSVLAACSTHF--RALFTVAEGdqtmnmiqldsevVTAEAFAALIDMMYTSTLMLGESN 93

                         90       100
                 ....*....|....*....|.
gi 157816985  90 LGNLEEAARALGVQALEEACK 110
Cdd:cd18196   94 VMDVLLAASHLHLNSVVKACK 114