N-terminal domain of Kruppel-like factor 2; Kruppel-like Factor 2 (KLF2, also known as Krueppel-like factor 2 or lung Kruppel-like Factor/LKLF) is a protein that, in humans, is encoded by the KLF2 gene on chromosome 19. It has been implicated in a variety of biochemical processes in the human body, including lung development, embryonic erythropoiesis, epithelial integrity, T-cell viability, and adipogenesis. KLF proteins KLF1, KLF2, KLF4, KLF5, KLF6, and KLF7 are transcriptional activators. KLF2 belongs to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specificity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF2, which is related to the N-terminal domains of KLF1 and KLF4.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 56090542   4 SEPILPSFATFASPCERGLQERWPRNEPEAGS-------------------TDEDLNSVLDFILSMGLDGLGAENPPEPP 64
Cdd:cd21583   1 SGTILPSISTFANQKEKCWEDRWKGEEDKSSLssclldlqngdsclgrkddEDLDNYLDLDFILANTAGSDSAGAAGGGY 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 56090542  65 PQPPPPAFYYPEP------------GAPPPYgtPAAGLGTELLR-PDLDAPQGPALHGRFLLAPPGRLVKAEP----PEV 127
Cdd:cd21583  81 YGSLPEPAAPYSAsnppgaysvpeqGSPPPP--YSSSLMAELLRdSDSDYGPSNSIQGRFLVSSAFFPRQDDPdsikVEP 158

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 56090542 128 DGGGYGCAAGLARGP-RGLKLEGalgaTGACMRGPagrppppsdtpplspdGPPRLPA---------------------- 184
Cdd:cd21583 159 SMDSYGPVMGMVPQScPKIKQEG----NVSCMMSF----------------EQPRLANspqaagsmtpplspddlmasec 218

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 56090542 185 PGPRNPFPPPFGPGPSFGGPGPALHYGPPAPGAFGLFDDAAAALGLAPpatRGLLTPPSSPLELLEAKPKRGRRSWPRKR 264
Cdd:cd21583 219 QQQMMCSPSFPQPHHPHYPPPPQPPLQYQSPHQFGLFEDGLPLQPSAQ---RVLLTPPSSPLELLESKPKRGRRSWPRKR 295

                ....
gi 56090542 265 AATH 268
Cdd:cd21583 296 TATH 299

N-terminal domain of Kruppel-like factor 2; Kruppel-like Factor 2 (KLF2, also known as Krueppel-like factor 2 or lung Kruppel-like Factor/LKLF) is a protein that, in humans, is encoded by the KLF2 gene on chromosome 19. It has been implicated in a variety of biochemical processes in the human body, including lung development, embryonic erythropoiesis, epithelial integrity, T-cell viability, and adipogenesis. KLF proteins KLF1, KLF2, KLF4, KLF5, KLF6, and KLF7 are transcriptional activators. KLF2 belongs to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specificity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF2, which is related to the N-terminal domains of KLF1 and KLF4.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 56090542   4 SEPILPSFATFASPCERGLQERWPRNEPEAGS-------------------TDEDLNSVLDFILSMGLDGLGAENPPEPP 64
Cdd:cd21583   1 SGTILPSISTFANQKEKCWEDRWKGEEDKSSLssclldlqngdsclgrkddEDLDNYLDLDFILANTAGSDSAGAAGGGY 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 56090542  65 PQPPPPAFYYPEP------------GAPPPYgtPAAGLGTELLR-PDLDAPQGPALHGRFLLAPPGRLVKAEP----PEV 127
Cdd:cd21583  81 YGSLPEPAAPYSAsnppgaysvpeqGSPPPP--YSSSLMAELLRdSDSDYGPSNSIQGRFLVSSAFFPRQDDPdsikVEP 158

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 56090542 128 DGGGYGCAAGLARGP-RGLKLEGalgaTGACMRGPagrppppsdtpplspdGPPRLPA---------------------- 184
Cdd:cd21583 159 SMDSYGPVMGMVPQScPKIKQEG----NVSCMMSF----------------EQPRLANspqaagsmtpplspddlmasec 218

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 56090542 185 PGPRNPFPPPFGPGPSFGGPGPALHYGPPAPGAFGLFDDAAAALGLAPpatRGLLTPPSSPLELLEAKPKRGRRSWPRKR 264
Cdd:cd21583 219 QQQMMCSPSFPQPHHPHYPPPPQPPLQYQSPHQFGLFEDGLPLQPSAQ---RVLLTPPSSPLELLESKPKRGRRSWPRKR 295

                ....
gi 56090542 265 AATH 268
Cdd:cd21583 296 TATH 299

Zinc-finger double domain;

                          10        20
                  ....*....|....*....|....*.
gi 56090542   314 ELTRHYRKHTGHRPFQCHLCDRAFSR 339
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPECGKSFKS 26

N-terminal domain of Kruppel-like factor 2; Kruppel-like Factor 2 (KLF2, also known as Krueppel-like factor 2 or lung Kruppel-like Factor/LKLF) is a protein that, in humans, is encoded by the KLF2 gene on chromosome 19. It has been implicated in a variety of biochemical processes in the human body, including lung development, embryonic erythropoiesis, epithelial integrity, T-cell viability, and adipogenesis. KLF proteins KLF1, KLF2, KLF4, KLF5, KLF6, and KLF7 are transcriptional activators. KLF2 belongs to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specificity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF2, which is related to the N-terminal domains of KLF1 and KLF4.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 56090542   4 SEPILPSFATFASPCERGLQERWPRNEPEAGS-------------------TDEDLNSVLDFILSMGLDGLGAENPPEPP 64
Cdd:cd21583   1 SGTILPSISTFANQKEKCWEDRWKGEEDKSSLssclldlqngdsclgrkddEDLDNYLDLDFILANTAGSDSAGAAGGGY 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 56090542  65 PQPPPPAFYYPEP------------GAPPPYgtPAAGLGTELLR-PDLDAPQGPALHGRFLLAPPGRLVKAEP----PEV 127
Cdd:cd21583  81 YGSLPEPAAPYSAsnppgaysvpeqGSPPPP--YSSSLMAELLRdSDSDYGPSNSIQGRFLVSSAFFPRQDDPdsikVEP 158

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 56090542 128 DGGGYGCAAGLARGP-RGLKLEGalgaTGACMRGPagrppppsdtpplspdGPPRLPA---------------------- 184
Cdd:cd21583 159 SMDSYGPVMGMVPQScPKIKQEG----NVSCMMSF----------------EQPRLANspqaagsmtpplspddlmasec 218

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 56090542 185 PGPRNPFPPPFGPGPSFGGPGPALHYGPPAPGAFGLFDDAAAALGLAPpatRGLLTPPSSPLELLEAKPKRGRRSWPRKR 264
Cdd:cd21583 219 QQQMMCSPSFPQPHHPHYPPPPQPPLQYQSPHQFGLFEDGLPLQPSAQ---RVLLTPPSSPLELLESKPKRGRRSWPRKR 295

                ....
gi 56090542 265 AATH 268
Cdd:cd21583 296 TATH 299

N-terminal domain of Kruppel-like factor 4; Kruppel-like factor 4 (KLF4; also known as Krueppel-like factor 4 or gut-enriched Kruppel-like factor/GKLF) is a protein that, in humans, is encoded by the KLF4 gene. Evidence also suggests that KLF4 is a tumor suppressor in certain cancers, including colorectal cancer, gastric cancer, esophageal squamous cell carcinoma, intestinal cancer, prostate cancer, bladder cancer and lung cancer. It may act as a tumor promoter where increased KLF4 expression has been reported, such as in oral squamous cell carcinoma and in primary breast ductal carcinoma. KLF4 is one of four key factors that are essential for inducing pluripotent stem cells. KLF4 is highly expressed in non-dividing cells and its overexpression induces cell cycle arrest. KLF proteins KLF1, KLF2, KLF4, KLF5, KLF6, and KLF7 are transcriptional activators. KLF4 belongs to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specificity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF4, which is related to the N-terminal domains of KLF1 and KLF2.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 56090542   1 MALSEPILPSFATFAS---------------------------PCErGLQERWPRNEPEAGST--DEDLNSVL--DFILS 49
Cdd:cd21582   4 MALSGTLLPSISTFASgpavkekalgqgsannrwreelshlkrPCP-PLGGRCYDQDPPLGMRrkEEEFNDLLdyDFILS 82

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 56090542  50 --MGLDGLGAENPPEPPPQPPPPAFYYPEPGAPPPYGT--------------------PAAGLGTELLRPDLDaP---QG 104
Cdd:cd21582  83 nsLLQQQQQQQQAASTSSSTSSSSSSPYSYQLPSPQGTatsflypipranladindvsPSGGFVAELMRPDLD-PaylQP 161

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 56090542 105 PALHGRFllappgrLVKAeppEVDGGGYG-----------------CAAGLARGPRGLKLEGALGATGAC-MRGPAGRPP 166
Cdd:cd21582 162 TSLHGKF-------VVKT---TMDMGDYSqsinvsksapmtkssvaPSSSLPFMCPRIKQENPSTCTISRpMDGHLGGNS 231

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 56090542 167 PPSDTPPLSPDGPPRLPAPGPRNPFPPPFGPGPSFGGPGPA--------LHYGPPAPGAFGLFDDAAAALGLAPPatrgL 238
Cdd:cd21582 232 QHGFSQRAPLPSRTTPSGGPGGGNSSTAESLMSRDHHPSSQvlshpplpLPQGYHPSPGYPPFPPPPSQPQQYQE----L 307

                       330       340       350
                ....*....|....*....|....*....|
gi 56090542 239 LTPPSSPLEllEAKPKRGRRSWPRKRAATH 268
Cdd:cd21582 308 MSPGSCLPE--EPKPKRGRRSWPRKRTATH 335

FOG: Zn-finger [General function prediction only];

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 56090542 266 ATHTCSYTNCGKTYTKSSHLKAHLRTHTGEKPYHCNWDGCGWKFARSDELTRHYRKHTGHRPFQCHLCdRAFSRSDHLAL 345
Cdd:COG5048 385 KKSETLSNSCIRNFKRDSNLSLHIITHLSFRPYNCKNPPCSKSFNRHYNLIPHKKIHTNHAPLLCSIL-KSFRRDLDLSN 463

                .
gi 56090542 346 H 346
Cdd:COG5048 464 H 464

FOG: Zn-finger [General function prediction only];

                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|
gi 56090542 274 NCGKTYTKSSHLKAHLRTHTGEKPYHCNWDGCGWKFARSDELTRHYRKHT 323
Cdd:COG5048  38 NCTDSFSRLEHLTRHIRSHTGEKPSQCSYSGCDKSFSRPLELSRHLRTHH 87

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.

                          10        20
                  ....*....|....*....|...
gi 56090542   328 FQCHLCDRAFSRSDHLALHMKRH 350
Cdd:pfam00096   1 YKCPDCGKSFSRKSNLKRHLRTH 23

FOG: Zn-finger [General function prediction only];

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 56090542 267 THTCSYTNCGKTYTKSSHLKAHLRTHTGEKPYHCNWDGCGWKF-----ARSDELTRHYRKHTGHRPFQC--HLCDRAFSR 339
Cdd:COG5048 321 PFSCPYSLCGKLFSRNDALKRHILLHTSISPAKEKLLNSSSKFspllnNEPPQSLQQYKDLKNDKKSETlsNSCIRNFKR 400

                        90
                ....*....|..
gi 56090542 340 SDHLALHMKRHM 351
Cdd:COG5048 401 DSNLSLHIITHL 412

N-terminal domain of Kruppel-like Factor 1; Kruppel-like Factor 1 (KLF1, also known as Krueppel-like factor 1 or Erythroid Kruppel-like Factor/EKLF) was the first Kruppel-like factor discovered. It was found to be vitally important for embryonic erythropoiesis in promoting the switch from fetal hemoglobin (Hemoglobin F) to adult hemoglobin (Hemoglobin A) gene expression by binding to highly conserved CACCC domains. EKLF ablation in mouse embryos produces a lethal anemic phenotype, causing death by embryonic day 14, and natural mutations lead to beta+ thalassemia in humans. However, expression of embryonic hemoglobin and fetal hemoglobin genes is normal in EKLF-deficient mice, suggesting other factors may be involved. KLF1 functions as a transcriptional activator. It belongs to a family of proteins, called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specifity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF1, which is related to the N-terminal domains of KLF2 and KLF4.

                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|
gi 56090542 219 GLFDDAAAALGLAPPATRGLLTPPSSPLELLEAKPKRGRRSWPRKRAATH 268
Cdd:cd21581 229 ALFPDYAHSPGPGHLPLGQQPLLPDPPLPPGGAEGKRGRRSWAKKRPAVH 278

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.

                          10        20
                  ....*....|....*....|....*
gi 56090542   298 YHCNwdGCGWKFARSDELTRHYRKH 322
Cdd:pfam00096   1 YKCP--DCGKSFSRKSNLKRHLRTH 23