|
Name |
Accession |
Description |
Interval |
E-value |
| PolY_Pol_eta |
cd01702 |
DNA Polymerase eta; Pol eta, also called Rad30A, is a translesion synthesis (TLS) polymerase. ... |
10-427 |
3.75e-161 |
|
DNA Polymerase eta; Pol eta, also called Rad30A, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Unlike other Y-family members, Pol eta can efficiently and accurately replicate DNA past UV-induced lesions. Its activity is initiated by two simultaneous interactions: the PIP box in pol eta interacting with PCNA, and the UBZ (ubiquitin-binding zinc finger) in pol eta interacting with monoubiquitin attached to PCNA. Pol eta is more efficient in copying damaged DNA than undamaged DNA and seems to recognize when a lesion has been passed, facilitating a lesion-dependent dissociation from the DNA.
Pssm-ID: 176456 [Multi-domain] Cd Length: 359 Bit Score: 467.95 E-value: 3.75e-161
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 10 ALVDMDCFFVQVEQRQNPHLRNKPCAVVQYKSwkgggIIAVSYEARAFGVTRNMWADDAKKLCPDLLLAQVR-------- 81
Cdd:cd01702 1 AHIDMDAFFAQVEQVRLGLLRNDPVAVVQWNS-----IIAVSYAARAFGVTRFMTIDEAKKKCPDLILAHVAtykkgede 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 82 --------ESRGKANLTKYREASVEVMEIMS-HFAVIERASIDEAYIDLtnavqerleklqgqpvsadllpstyieglpr 152
Cdd:cd01702 76 adyhenpsPARHKVSLDPYRRASRKILNILKrFGDVVEKASIDEAYLDL------------------------------- 124
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 153 gpavedtvekedirkqgllqwlgslekddptspdlrltvGAVIVEEMRAAIERKTGFQCSAGISHNKVLAKLACGLNKPN 232
Cdd:cd01702 125 ---------------------------------------GSRIVEEIRQQVYDELGYTCSAGIAHNKMLAKLASGMNKPN 165
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 233 RQTLVSHGSVPQLFSQMPIRKIRSLGGKLGASVIDVLGVEYMGDLTQF--TEAQLQSHFGEKNGSWLYAMCRGIEHEPVK 310
Cdd:cd01702 166 AQTILRNDAVASFLSSLPITSIRGLGGKLGEEIIDLLGLPTEGDVAGFrsSESDLQEHFGEKLGEWLYNLLRGIDHEPVK 245
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 311 PRQLPKTIGCSKNFPGKTALATrEQVQWWLLQLALELEERLTKDRTDNGRVATQLVVSIRVEGDKrlSSLRRCCALTRYD 390
Cdd:cd01702 246 PRPLPKSMGSSKNFPGKTALST-EDVQHWLLVLASELNSRLEDDRYENNRRPKTLVLSLRQRGDG--VRRSRSCALPRYD 322
|
410 420 430
....*....|....*....|....*....|....*..
gi 157817057 391 AHKMSQDAFATIRNCNTSGVQTEWSPPLTMLFLCATK 427
Cdd:cd01702 323 AQKIVKDAFKLIKAINEEGLGLAWNYPLTLLSLSFTK 359
|
|
| DinP |
COG0389 |
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination ... |
8-337 |
1.01e-68 |
|
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination and repair];
Pssm-ID: 440158 [Multi-domain] Cd Length: 336 Bit Score: 228.10 E-value: 1.01e-68
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 8 VVALVDMDCFFVQVEQRQNPHLRNKPCAVVqykSWKGGG-IIAVSYEARAFGVTRNMWADDAKKLCPDLLLAqvresrgK 86
Cdd:COG0389 2 RILHVDMDAFYASVEQRDRPELRGKPVAVG---GDNNRGvVAAASYEARAFGVRSGMPLFQARRLCPDLVVL-------P 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 87 ANLTKYREASVEVMEIMSHFA-VIERASIDEAYIDLTnavqerleklqgqpvsadllpstyieglprgpavedtvekedi 165
Cdd:COG0389 72 PDFELYRDVSRRVMAILERYTpLVEPLSIDEAFLDVT------------------------------------------- 108
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 166 rkqGLLQWLGSLEKddptspdlrltvgavIVEEMRAAIERKTGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQL 245
Cdd:COG0389 109 ---GSARLFGSAEA---------------IARRIRRRIRRETGLTVSVGIAPNKFLAKIASDLAKPDGLTVIPPGEVAAF 170
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 246 FSQMPIRKIRSLGGKLGASVIDvLGVEYMGDLTQFTEAQLQSHFGeKNGSWLYAMCRGIEHEPVKPRQLPKTIGCSKNFP 325
Cdd:COG0389 171 LAPLPVEKLWGVGPKTAEKLAR-LGIRTIGDLAALPRAELRRRFG-KVGERLYRLARGIDPRPVEPRRPRKSIGVERTFG 248
|
330
....*....|..
gi 157817057 326 gkTALATREQVQ 337
Cdd:COG0389 249 --EDLTDLEELE 258
|
|
| PRK02406 |
PRK02406 |
DNA polymerase IV; Validated |
14-319 |
2.20e-56 |
|
DNA polymerase IV; Validated
Pssm-ID: 235035 [Multi-domain] Cd Length: 343 Bit Score: 195.34 E-value: 2.20e-56
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 14 MDCFFVQVEQRQNPHLRNKPCAVvqykswkGG-----GIIAV-SYEARAFGVTRNMWADDAKKLCPDLLLAQVResrgka 87
Cdd:PRK02406 1 MDCFYAAVEMRDNPELRGKPVAV-------GGspgrrGVISTcNYEARKFGVRSAMPTAQALKLCPDLIFVPGR------ 67
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 88 nLTKYREASVEVMEIMSHF-AVIERASIDEAYIDLTNAVQerleklqgqpvsadllpstyieglPRGPAVEdtvekedir 166
Cdd:PRK02406 68 -FDVYKEVSRQIREIFRRYtDLIEPLSLDEAYLDVTDNKL------------------------CIGSATL--------- 113
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 167 kqgllqwlgslekddptspdlrltvgavIVEEMRAAIERKTGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQLF 246
Cdd:PRK02406 114 ----------------------------IAQEIRQDIFEELGLTASAGVAPNKFLAKIASDWNKPNGLFVITPEEVDAFL 165
|
250 260 270 280 290 300 310
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 157817057 247 SQMPIRKIRslG-GKLGASVIDVLGVEYMGDLTQFTEAQLQSHFGeKNGSWLYAMCRGIEHEPVKPRQLPKTIG 319
Cdd:PRK02406 166 ATLPVEKIP--GvGKVTAEKLHALGIYTCADLQKYDLAELIRHFG-KFGRRLYERARGIDERPVKPDRERKSVG 236
|
|
| IMS |
pfam00817 |
impB/mucB/samB family; These proteins are involved in UV protection (Swiss). |
12-226 |
6.07e-44 |
|
impB/mucB/samB family; These proteins are involved in UV protection (Swiss).
Pssm-ID: 425885 [Multi-domain] Cd Length: 148 Bit Score: 154.65 E-value: 6.07e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 12 VDMDCFFVQVEQRQNPHLRNKPCAVVQYKSwkGGGIIAVSYEARAFGVTRNMWADDAKKLCPDLLLAqvresrgKANLTK 91
Cdd:pfam00817 1 IDMDAFFASVELLRDPELKGKPVAVGGGNG--RGIVAAASYEARKYGVRSGMPVFEAKKLCPNLIVV-------PPDLEL 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 92 YREASVEVMEIMSHFA--VIERASIDEAYIDLTnavqeRLEKLQGQPVSadllpstyieglprgpavedtvekedirkqg 169
Cdd:pfam00817 72 YRRASRKIFEILRRFStpKVEQASIDEAFLDLT-----GLEKLFGAEEA------------------------------- 115
|
170 180 190 200 210
....*....|....*....|....*....|....*....|....*....|....*..
gi 157817057 170 llqwlgslekddptspdlrltvgavIVEEMRAAIERKTGFQCSAGISHNKVLAKLAC 226
Cdd:pfam00817 116 -------------------------LAKRLRREIAEETGLTCSIGIAPNKLLAKLAS 147
|
|
| zf_UBZ |
pfam18439 |
Ubiquitin-Binding Zinc Finger; This is ubiquitin-binding zinc finger (UBZ) domain found in DNA ... |
608-638 |
2.50e-11 |
|
Ubiquitin-Binding Zinc Finger; This is ubiquitin-binding zinc finger (UBZ) domain found in DNA polymerase eta (EC:2.7.7.7). It is important in the recruitment of the polymerase to the stalled replication machinery in translesion synthesis. The UBZ domain adopts a classical C2H2 zinc-finger structure characterized by a beta-beta-alpha fold.
Pssm-ID: 465769 Cd Length: 32 Bit Score: 58.68 E-value: 2.50e-11
10 20 30
....*....|....*....|....*....|.
gi 157817057 608 DQVLCEKCDSLVPVWDMPEHLDYHFALELQK 638
Cdd:pfam18439 1 DTYVCPRCGKEIPESELPEHEDWHFAKDLQR 31
|
|
| |
|
Name |
Accession |
Description |
Interval |
E-value |
| PolY_Pol_eta |
cd01702 |
DNA Polymerase eta; Pol eta, also called Rad30A, is a translesion synthesis (TLS) polymerase. ... |
10-427 |
3.75e-161 |
|
DNA Polymerase eta; Pol eta, also called Rad30A, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Unlike other Y-family members, Pol eta can efficiently and accurately replicate DNA past UV-induced lesions. Its activity is initiated by two simultaneous interactions: the PIP box in pol eta interacting with PCNA, and the UBZ (ubiquitin-binding zinc finger) in pol eta interacting with monoubiquitin attached to PCNA. Pol eta is more efficient in copying damaged DNA than undamaged DNA and seems to recognize when a lesion has been passed, facilitating a lesion-dependent dissociation from the DNA.
Pssm-ID: 176456 [Multi-domain] Cd Length: 359 Bit Score: 467.95 E-value: 3.75e-161
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 10 ALVDMDCFFVQVEQRQNPHLRNKPCAVVQYKSwkgggIIAVSYEARAFGVTRNMWADDAKKLCPDLLLAQVR-------- 81
Cdd:cd01702 1 AHIDMDAFFAQVEQVRLGLLRNDPVAVVQWNS-----IIAVSYAARAFGVTRFMTIDEAKKKCPDLILAHVAtykkgede 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 82 --------ESRGKANLTKYREASVEVMEIMS-HFAVIERASIDEAYIDLtnavqerleklqgqpvsadllpstyieglpr 152
Cdd:cd01702 76 adyhenpsPARHKVSLDPYRRASRKILNILKrFGDVVEKASIDEAYLDL------------------------------- 124
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 153 gpavedtvekedirkqgllqwlgslekddptspdlrltvGAVIVEEMRAAIERKTGFQCSAGISHNKVLAKLACGLNKPN 232
Cdd:cd01702 125 ---------------------------------------GSRIVEEIRQQVYDELGYTCSAGIAHNKMLAKLASGMNKPN 165
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 233 RQTLVSHGSVPQLFSQMPIRKIRSLGGKLGASVIDVLGVEYMGDLTQF--TEAQLQSHFGEKNGSWLYAMCRGIEHEPVK 310
Cdd:cd01702 166 AQTILRNDAVASFLSSLPITSIRGLGGKLGEEIIDLLGLPTEGDVAGFrsSESDLQEHFGEKLGEWLYNLLRGIDHEPVK 245
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 311 PRQLPKTIGCSKNFPGKTALATrEQVQWWLLQLALELEERLTKDRTDNGRVATQLVVSIRVEGDKrlSSLRRCCALTRYD 390
Cdd:cd01702 246 PRPLPKSMGSSKNFPGKTALST-EDVQHWLLVLASELNSRLEDDRYENNRRPKTLVLSLRQRGDG--VRRSRSCALPRYD 322
|
410 420 430
....*....|....*....|....*....|....*..
gi 157817057 391 AHKMSQDAFATIRNCNTSGVQTEWSPPLTMLFLCATK 427
Cdd:cd01702 323 AQKIVKDAFKLIKAINEEGLGLAWNYPLTLLSLSFTK 359
|
|
| DinP |
COG0389 |
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination ... |
8-337 |
1.01e-68 |
|
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination and repair];
Pssm-ID: 440158 [Multi-domain] Cd Length: 336 Bit Score: 228.10 E-value: 1.01e-68
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 8 VVALVDMDCFFVQVEQRQNPHLRNKPCAVVqykSWKGGG-IIAVSYEARAFGVTRNMWADDAKKLCPDLLLAqvresrgK 86
Cdd:COG0389 2 RILHVDMDAFYASVEQRDRPELRGKPVAVG---GDNNRGvVAAASYEARAFGVRSGMPLFQARRLCPDLVVL-------P 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 87 ANLTKYREASVEVMEIMSHFA-VIERASIDEAYIDLTnavqerleklqgqpvsadllpstyieglprgpavedtvekedi 165
Cdd:COG0389 72 PDFELYRDVSRRVMAILERYTpLVEPLSIDEAFLDVT------------------------------------------- 108
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 166 rkqGLLQWLGSLEKddptspdlrltvgavIVEEMRAAIERKTGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQL 245
Cdd:COG0389 109 ---GSARLFGSAEA---------------IARRIRRRIRRETGLTVSVGIAPNKFLAKIASDLAKPDGLTVIPPGEVAAF 170
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 246 FSQMPIRKIRSLGGKLGASVIDvLGVEYMGDLTQFTEAQLQSHFGeKNGSWLYAMCRGIEHEPVKPRQLPKTIGCSKNFP 325
Cdd:COG0389 171 LAPLPVEKLWGVGPKTAEKLAR-LGIRTIGDLAALPRAELRRRFG-KVGERLYRLARGIDPRPVEPRRPRKSIGVERTFG 248
|
330
....*....|..
gi 157817057 326 gkTALATREQVQ 337
Cdd:COG0389 249 --EDLTDLEELE 258
|
|
| PolY_Pol_IV_kappa |
cd03586 |
DNA Polymerase IV/Kappa; Pol IV, also known as Pol kappa, DinB, and Dpo4, is a translesion ... |
12-328 |
4.13e-67 |
|
DNA Polymerase IV/Kappa; Pol IV, also known as Pol kappa, DinB, and Dpo4, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Known primarily as Pol IV in prokaryotes and Pol kappa in eukaryotes, this polymerase has a propensity for generating frameshift mutations. The eukaryotic Pol kappa differs from Pol IV and Dpo4 by an N-terminal extension of ~75 residues known as the "N-clasp" region. The structure of Pol kappa shows DNA that is almost totally encircled by Pol kappa, with the N-clasp region augmenting the interactions between DNA and the polymerase. Pol kappa is more resistant than Pol eta and Pol iota to bulky guanine adducts and is efficient at catalyzing the incorporation of dCTP. Bacterial pol IV has a higher error rate than other Y-family polymerases.
Pssm-ID: 176459 [Multi-domain] Cd Length: 334 Bit Score: 223.55 E-value: 4.13e-67
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 12 VDMDCFFVQVEQRQNPHLRNKPCAVvqykswkGG----GIIA-VSYEARAFGVTRNMWADDAKKLCPDLLLAqvresrgK 86
Cdd:cd03586 3 IDMDAFYASVEQRDNPELKGKPVAV-------GGssdrGVVStASYEARKFGVRSAMPIFQAKKLCPNLIFV-------P 68
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 87 ANLTKYREASVEVMEIMS-HFAVIERASIDEAYIDLTNAVQERleklqgqpvsadllpstyieglprGPAVEdtvekedi 165
Cdd:cd03586 69 PRFDKYREVSRQIMEILReYTPLVEPLSIDEAYLDVTDYVRLF------------------------GSATE-------- 116
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 166 rkqgllqwlgslekddptspdlrltvgavIVEEMRAAIERKTGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQL 245
Cdd:cd03586 117 -----------------------------IAKEIRARIREETGLTASAGIAPNKFLAKIASDLNKPNGLTVIPPEDVEEF 167
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 246 FSQMPIRKIRSLGGKLGASVIDvLGVEYMGDLTQFTEAQLQSHFGeKNGSWLYAMCRGIEHEPVKPRQLPKTIGCSKNFP 325
Cdd:cd03586 168 LAPLPVRKIPGVGKVTAEKLKE-LGIKTIGDLAKLDVELLKKLFG-KSGRRLYELARGIDNRPVEPDRERKSIGVERTFS 245
|
...
gi 157817057 326 GKT 328
Cdd:cd03586 246 EDL 248
|
|
| PRK02406 |
PRK02406 |
DNA polymerase IV; Validated |
14-319 |
2.20e-56 |
|
DNA polymerase IV; Validated
Pssm-ID: 235035 [Multi-domain] Cd Length: 343 Bit Score: 195.34 E-value: 2.20e-56
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 14 MDCFFVQVEQRQNPHLRNKPCAVvqykswkGG-----GIIAV-SYEARAFGVTRNMWADDAKKLCPDLLLAQVResrgka 87
Cdd:PRK02406 1 MDCFYAAVEMRDNPELRGKPVAV-------GGspgrrGVISTcNYEARKFGVRSAMPTAQALKLCPDLIFVPGR------ 67
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 88 nLTKYREASVEVMEIMSHF-AVIERASIDEAYIDLTNAVQerleklqgqpvsadllpstyieglPRGPAVEdtvekedir 166
Cdd:PRK02406 68 -FDVYKEVSRQIREIFRRYtDLIEPLSLDEAYLDVTDNKL------------------------CIGSATL--------- 113
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 167 kqgllqwlgslekddptspdlrltvgavIVEEMRAAIERKTGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQLF 246
Cdd:PRK02406 114 ----------------------------IAQEIRQDIFEELGLTASAGVAPNKFLAKIASDWNKPNGLFVITPEEVDAFL 165
|
250 260 270 280 290 300 310
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 157817057 247 SQMPIRKIRslG-GKLGASVIDVLGVEYMGDLTQFTEAQLQSHFGeKNGSWLYAMCRGIEHEPVKPRQLPKTIG 319
Cdd:PRK02406 166 ATLPVEKIP--GvGKVTAEKLHALGIYTCADLQKYDLAELIRHFG-KFGRRLYERARGIDERPVKPDRERKSVG 236
|
|
| PolY |
cd00424 |
Y-family of DNA polymerases; Y-family DNA polymerases are a specialized subset of polymerases ... |
12-395 |
2.50e-45 |
|
Y-family of DNA polymerases; Y-family DNA polymerases are a specialized subset of polymerases that facilitate translesion synthesis (TLS), a process that allows the bypass of a variety of DNA lesions. Unlike replicative polymerases, TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. The active sites of TLS polymerases are large and flexible to allow the accomodation of distorted bases. Most TLS polymerases are members of the Y-family, including Pol eta, Pol kappa/IV, Pol iota, Rev1, and Pol V, which is found exclusively in bacteria. In eukaryotes, the B-family polymerase Pol zeta also functions as a TLS polymerase. Expression of Y-family polymerases is often induced by DNA damage and is believed to be highly regulated. TLS is likely induced by the monoubiquitination of the replication clamp PCNA, which provides a scaffold for TLS polymerases to bind in order to access the lesion. Because of their high error rates, TLS polymerases are potential targets for cancer treatment and prevention.
Pssm-ID: 176453 [Multi-domain] Cd Length: 343 Bit Score: 165.23 E-value: 2.50e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 12 VDMDCFFVQVEQRQNPHLRNKPCAVVQYKSwKGGGIIAVSYEARAFGVTRNMWADDAKKLCPDLLLAqvresrgKANLTK 91
Cdd:cd00424 3 IDFDNFFASVEQLARPELKGRPVVVVPFNS-DSTCVIACSYEARKYGVKRGMPVREARKMCPNLILV-------PARLDL 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 92 YREASVEVMEIMSHFA-VIERASIDEAYIDLTNAVQErleklqgqpvsadllpstyieglprgpavedtvekedirkqgl 170
Cdd:cd00424 75 YRRLSERLLSELEEVApLVEVASIDELFLDLTGSARL------------------------------------------- 111
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 171 lqwlgslekddptspdlrLTVGAVIVEEMRAAIERKTG-FQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQLFSQM 249
Cdd:cd00424 112 ------------------LGLGSEVALRIKRHIAEQLGgITASIGIASNKLLAKLAAKYAKPDGLTILDPEDLPGFLSKL 173
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 250 PIRKIRSLGGKLGASVIDVlGVEYMGDLTQFTEAQLQSHFGEKNGSWLYAMCRGIEHEPVKPRQLPKTIGCSKNFPGKta 329
Cdd:cd00424 174 PLTDLPGIGAVTAKRLEAV-GINPIGDLLAASPDALLALWGGVSGERLWYALRGIDDEPLSPPRPRKSFSHERVLPRD-- 250
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 157817057 330 LATREQVQWWLLQLALELEERLTKDrtdnGRVATQLVVSIR-VEGDKRLSSLRRCCALTRYDAHKMS 395
Cdd:cd00424 251 SRNAEDARPLLRLLLEKLARRLRRD----GRGATRLRLWLRtVDGRWSGHADIPSRSAPRPISTEDG 313
|
|
| IMS |
pfam00817 |
impB/mucB/samB family; These proteins are involved in UV protection (Swiss). |
12-226 |
6.07e-44 |
|
impB/mucB/samB family; These proteins are involved in UV protection (Swiss).
Pssm-ID: 425885 [Multi-domain] Cd Length: 148 Bit Score: 154.65 E-value: 6.07e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 12 VDMDCFFVQVEQRQNPHLRNKPCAVVQYKSwkGGGIIAVSYEARAFGVTRNMWADDAKKLCPDLLLAqvresrgKANLTK 91
Cdd:pfam00817 1 IDMDAFFASVELLRDPELKGKPVAVGGGNG--RGIVAAASYEARKYGVRSGMPVFEAKKLCPNLIVV-------PPDLEL 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 92 YREASVEVMEIMSHFA--VIERASIDEAYIDLTnavqeRLEKLQGQPVSadllpstyieglprgpavedtvekedirkqg 169
Cdd:pfam00817 72 YRRASRKIFEILRRFStpKVEQASIDEAFLDLT-----GLEKLFGAEEA------------------------------- 115
|
170 180 190 200 210
....*....|....*....|....*....|....*....|....*....|....*..
gi 157817057 170 llqwlgslekddptspdlrltvgavIVEEMRAAIERKTGFQCSAGISHNKVLAKLAC 226
Cdd:pfam00817 116 -------------------------LAKRLRREIAEETGLTCSIGIAPNKLLAKLAS 147
|
|
| PolY_Rev1 |
cd01701 |
DNA polymerase Rev1; Rev1 is a translesion synthesis (TLS) polymerase found in eukaryotes. ... |
3-325 |
5.94e-39 |
|
DNA polymerase Rev1; Rev1 is a translesion synthesis (TLS) polymerase found in eukaryotes. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Rev1 has both structural and enzymatic roles. Structurally, it is believed to interact with other nonclassical polymerases and replication machinery to act as a scaffold. Enzymatically, it catalyzes the specific insertion of dCMP opposite abasic sites. Rev1 interacts with the Rev7 subunit of the B-family TLS polymerase Pol zeta (Rev3/Rev7). Rev1 is known to actively promote the introduction of mutations, potentially making it a significant target for cancer treatment.
Pssm-ID: 176455 [Multi-domain] Cd Length: 404 Bit Score: 148.62 E-value: 5.94e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 3 PGQDLVVALVDMDCFFVQVEQRQNPHLRNKPCAVVQykswkGGG----IIAVSYEARAFGVTRNMWADDAKKLCPDLLLA 78
Cdd:cd01701 43 PDLQRIIMHVDFDCFFVSVSIRNRPDLKGKPVAVCH-----GKGpnseIASCNYEARSYGIKNGMWVGQAKKLCPQLVTL 117
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 79 QVresrgkaNLTKYREASVEVMEI-MSHFAVIERASIDEAYIDLTNAVQErleklqgqpvsadllpstyieglprgpave 157
Cdd:cd01701 118 PY-------DFEAYEEVSLTFYEIlASYTDNIEAVSCDEALIDITSLLEE------------------------------ 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 158 dtvekedirkqgllqwlgslEKDDPTspdlrltvgaVIVEEMRAAIERKTGFQCSAGISHNKVLAKLACGLNKPNRQTLV 237
Cdd:cd01701 161 --------------------TYELPE----------ELAEAIRNEIRETTGCSASVGIGPNILLARLATRKAKPDGQYHL 210
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 238 SHGSVPQLFSQMPIRKIRSLGGKLGASVIDVLGV-EYMGDLTQFTEAQLQSHFGEKNGSWLYAMCRGIEHEPVKPRQLPK 316
Cdd:cd01701 211 SAEKVEEFLSQLKVGDLPGVGSSLAEKLVKLFGDtCGGLELRSKTKEKLQKVLGPKTGEKLYDYCRGIDDRPVTGEKERK 290
|
....*....
gi 157817057 317 TIGCSKNFP 325
Cdd:cd01701 291 SVSAEINYG 299
|
|
| PolY_Pol_iota |
cd01703 |
DNA Polymerase iota; Pol iota, also called Rad30B, is a translesion synthesis (TLS) polymerase. ... |
11-336 |
1.59e-37 |
|
DNA Polymerase iota; Pol iota, also called Rad30B, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Pol iota is thought to be one of the least efficient polymerases, particularly when opposite pyrimidines; it can incorporate the correct nucleotide opposite a purine much more efficiently than opposite a pyrimidine, and prefers to insert guanosine instead of adenosine opposite thymidine. Pol iota is believed to use Hoogsteen rather than Watson-Crick base pairing, which may explain the varying efficiency for different template nucleotides.
Pssm-ID: 176457 [Multi-domain] Cd Length: 379 Bit Score: 144.15 E-value: 1.59e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 11 LVDMDCFFVQVEQRQNPHLRNKPCAVVQyKSwkgggIIA-VSYEARAFGVTRNMWADDAKKLCPDLLLAQvresrGKaNL 89
Cdd:cd01703 2 HLDLDCFYAQVEEIRDPSLKSKPLGIQQ-KY-----IVVtCNYEARRLGVKKLMSIKDAKEICPDLVLVN-----GE-DL 69
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 90 TKYREASVEVMEIMSHFA---VIERASIDEAYIDLTnavqerleklqgqpvsadllpstyieglprgpavedtvekedir 166
Cdd:cd01703 70 TPFRDMSKKVYRLLRSYSwndRVERLGFDENFMDVT-------------------------------------------- 105
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 167 kqgllqwlgslekddptspDLRLTVGAVIVEEMRAAIERKTGFQCSAGISHNKVLAKLACGLNKPNRQT-LVSHGS--VP 243
Cdd:cd01703 106 -------------------EMRLLVASHIAYEMRERIENELGLTCCAGIASNKLLAKLVGSVNKPNQQTtLLPPSCadLM 166
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 244 QLFSQMPIRKIRSLGGKLGASVIDVL------GVEYMGDLTQFTEAQ--------LQSHFGEKNGSWLYAMCRGIEHEPV 309
Cdd:cd01703 167 DFMDLHDLRKIPGIGYKTAAKLEAHGissvrdLQEFSNRNRQTVGAApsllelllMVKEFGEGIGQRIWKLLFGRDTSPV 246
|
330 340
....*....|....*....|....*...
gi 157817057 310 KP-RQLPKTIGCSKNFPgKTALATREQV 336
Cdd:cd01703 247 KPaSDFPQQISIEDSYK-KCSLEEIREA 273
|
|
| PRK01810 |
PRK01810 |
DNA polymerase IV; Validated |
12-325 |
1.12e-35 |
|
DNA polymerase IV; Validated
Pssm-ID: 179337 [Multi-domain] Cd Length: 407 Bit Score: 139.39 E-value: 1.12e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 12 VDMDCFFVQVEQRQNPHLRNKPCAVVQYKSWKGGGIIAVSYEARAFGV--TRNMWadDAKKLCPDLLLAqvresrgKANL 89
Cdd:PRK01810 10 VDMNSFFASVEIAYDPSLQGKPLAVAGNEKERKGIIVTCSYEARAYGIrtTMPLW--EAKRLCPQLIVR-------RPNF 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 90 TKYREASVEVMEIMSHFA-VIERASIDEAYIDLTnavqerleklqgqpvsadllpstyieglprgpaveDTVEKEDirkq 168
Cdd:PRK01810 81 DRYREASRQMFQILSEFTpLVQPVSIDEGYLDIT-----------------------------------DCYALGS---- 121
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 169 gllqwlgSLEkddptspdlrltvgavIVEEMRAAIERKTGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQLFSQ 248
Cdd:PRK01810 122 -------PLE----------------IAKMIQQRLLTELQLPCSIGIAPNKFLAKMASDMKKPLGITVLRKRDVPEMLWP 178
|
250 260 270 280 290 300 310
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 157817057 249 MPIRKIRSLGGKLgASVIDVLGVEYMGDLTQFTEAQLQSHFGeKNGSWLYAMCRGIEHEPVKPRQLP--KTIGCSKNFP 325
Cdd:PRK01810 179 LPVGEMHGIGEKT-AEKLKDIGIQTIGDLAKADEHILRAKLG-INGVRLQRRANGIDDRPVDPEAIYqfKSVGNSTTLS 255
|
|
| PRK14133 |
PRK14133 |
DNA polymerase IV; Provisional |
6-319 |
1.55e-35 |
|
DNA polymerase IV; Provisional
Pssm-ID: 184529 [Multi-domain] Cd Length: 347 Bit Score: 137.54 E-value: 1.55e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 6 DLVVALVDMDCFFVQVEQRQNPHLRNKPCAVvqykswkGG----GIIAV-SYEARAFGVTRNMWADDAKKLCPDLLLAQV 80
Cdd:PRK14133 2 DRVIIHVDMDAFFASVEQMDNPKLKGKPVIV-------GGiserGVVSTcSYEARKYGVHSAMPVFMAKKRCPHGIFLPV 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 81 RESRgkanltkYREASVEVMEIMSHFA-VIERASIDEAYIDLTNavqerleklqgqpvsadllpstyieglprgpavedt 159
Cdd:PRK14133 75 RHER-------YKEVSKNIFKILYEVTpIVEPVSIDEAYLDITN------------------------------------ 111
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 160 VEKEDIRkqgllqwlgslekddptspdlrltvgavIVEEMRAAIERKTGFQCSAGISHNKVLAKLACGLNKPNRQTLVSH 239
Cdd:PRK14133 112 IKEEPIK----------------------------IAKYIKKKVKKETGLTLSVGISYNKFLAKLASDWNKPDGIKIITE 163
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 240 GSVPQLFSQMPIRKIRSLGGKLGASVIDVlGVEYMGDLTQFTEAQLQSHFGeKNGSWLYAMCRGIEHEPVKPRQLPKTIG 319
Cdd:PRK14133 164 DMIPDILKPLPISKVHGIGKKSVEKLNNI-GIYTIEDLLKLSREFLIEYFG-KFGVEIYERIRGIDYREVEVSRERKSIG 241
|
|
| PRK03103 |
PRK03103 |
DNA polymerase IV; Reviewed |
8-319 |
2.92e-35 |
|
DNA polymerase IV; Reviewed
Pssm-ID: 235104 [Multi-domain] Cd Length: 409 Bit Score: 138.21 E-value: 2.92e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 8 VVALVDMDCFFVQVEQRQNPHLRNKPCAVVQYKSWKGGGIIAVSYEARAFGVTRNMWADDAKKLCPDLLLAQVRESRgka 87
Cdd:PRK03103 4 VILLVDMQSFYASVEKAANPELKGRPVIVSGDPERRSGVVLAACPLAKAYGVKTAERLWEAQQKCPDLVVVKPRMQR--- 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 88 nltkYREASVEVMEIMSHFA-VIERASIDEAYIDLTNAVqerleKLQGQPVSadllpstyieglprgpavedtvekedir 166
Cdd:PRK03103 81 ----YIDVSLQITRILEDFTdLVEPFSIDEQFLDVTGSQ-----KLFGSPLE---------------------------- 123
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 167 kqgllqwlgslekddptspdlrltvgavIVEEMRAAIERKTGFQCSAGISHNKVLAKLACGL---NKPNRQTLVSHGSVP 243
Cdd:PRK03103 124 ----------------------------IAQKIQQRIMRETGVYARVGIGPNKLLAKMACDNfakKNPDGLFTLDKEDVP 175
|
250 260 270 280 290 300 310
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 157817057 244 QLFSQMPIRKIRSLGGKLGASVIDvLGVEYMGDLTQFTEAQLQSHFGeKNGSWLYAMCRGIEHEPVKPRQLP--KTIG 319
Cdd:PRK03103 176 ADLWPLPVRKLFGVGSRMEKHLRR-MGIRTIGQLANTPLERLKKRWG-INGEVLWRTANGIDYSPVTPHSLDrqKAIG 251
|
|
| PRK03348 |
PRK03348 |
DNA polymerase IV; Provisional |
9-336 |
1.19e-33 |
|
DNA polymerase IV; Provisional
Pssm-ID: 235118 [Multi-domain] Cd Length: 454 Bit Score: 134.68 E-value: 1.19e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 9 VALVDMDCFFVQVEQRQNPHLRNKPCAVvqykswkGG----GIIA-VSYEARAFGVTRNMWADDAKKLCPdlLLAQVRES 83
Cdd:PRK03348 7 VLHLDMDAFFASVEQLTRPTLRGRPVLV-------GGlggrGVVAgASYEARVFGARSAMPMHQARRLVG--NGAVVLPP 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 84 RGKAnltkYREASVEVMEIM-SHFAVIERASIDEAYidltnavqerLEKLQGQPVSADllpstyieglprgpAVEDtvek 162
Cdd:PRK03348 78 RFVV----YRAASRRVFDTLrELSPVVEQLSFDEAF----------VEPAELAGASAE--------------EVEA---- 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 163 edirkqgllqwlgslekddptspdlrltvgavIVEEMRAAIERKTGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSV 242
Cdd:PRK03348 126 --------------------------------FAERLRARVREETGLPASVGAGSGKQIAKIASGLAKPDGIRVVPPGEE 173
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 243 PQLFSQMPIRKIRSLG----GKLGAsvidvLGVEYMGDLTQFTEAQLQSHFGEKNGSWLYAMCRGIEHEPVKPRQLPKTI 318
Cdd:PRK03348 174 RELLAPLPVRRLWGIGpvteEKLHR-----LGIETIGDLAALSEAEVANLLGATVGPALHRLARGIDDRPVAERAEAKQI 248
|
330
....*....|....*...
gi 157817057 319 GCSKNFPgkTALATREQV 336
Cdd:PRK03348 249 SAESTFA--VDLTTRAQL 264
|
|
| PRK01216 |
PRK01216 |
DNA polymerase IV; Validated |
7-312 |
1.15e-29 |
|
DNA polymerase IV; Validated
Pssm-ID: 179251 [Multi-domain] Cd Length: 351 Bit Score: 120.66 E-value: 1.15e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 7 LVVALVDMDCFFVQVEQRQNPHLRNKPCAVVQY--KSWKGGGIIAVSYEARAFGVTRNMWADDAKKLCPDLLLAQVRESr 84
Cdd:PRK01216 1 MIILFVDFDYFFAQVEEVLNPSLKGKPVVVCVYsgRFEDSGAVATANYEARKLGIKAGMPIVEAKKILPNAVYLPMRKE- 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 85 gkanltKYREASVEVMEIMSHFA-VIERASIDEAYIDLTNAVQERLEKLQgqpvsadllpstyieglprgpavedtveke 163
Cdd:PRK01216 80 ------VYQQVSNRIMKLLREYSeKIEIASIDEAYLDISDKVKNYQDAYN------------------------------ 123
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 164 dirkqgllqwlgslekddptspdlrltvgavIVEEMRAAIERKTGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVP 243
Cdd:PRK01216 124 -------------------------------LGLEIKNKILEKEKITVTVGISKNKVFAKIAADMAKPNGIKVIDDEEVK 172
|
250 260 270 280 290 300
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 157817057 244 QLFSQMPIRKIRSLgGKLGASVIDVLGVEYMGDLTQFTEAQLQSHFGEKNGSWLYAMCRGIEHEPVKPR 312
Cdd:PRK01216 173 RFINELDIADIPGI-GDITAEKLKKLGVNKLVDTLRIEFDELKGIIGEAKAKYLFSLARNEYNEPVRAR 240
|
|
| PolY_Pol_V_umuC |
cd01700 |
umuC subunit of DNA Polymerase V; umuC subunit of Pol V. Pol V is a bacterial translesion ... |
10-325 |
2.40e-27 |
|
umuC subunit of DNA Polymerase V; umuC subunit of Pol V. Pol V is a bacterial translesion synthesis (TLS) polymerase that consists of the heterotrimer of one umuC and two umuD subunits. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Pol V, RecA, single stranded DNA-binding protein, beta sliding clamp, and gamma clamp loading complex are responsible for inducing the SOS response in bacteria to repair UV-induced DNA damage.
Pssm-ID: 176454 [Multi-domain] Cd Length: 344 Bit Score: 113.80 E-value: 2.40e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 10 ALVDMDCFFVQVEQRQNPHLRNKPCAVVqykSWKGGGIIAVSYEARAFGVTRNMWADDAKKLCPDLLLAqVRESrgkaNL 89
Cdd:cd01700 1 ALVDCNSFYASCERVFRPLLLGRPLVVL---SNNDGCVIARSPEAKALGIKMGSPYFKVPDLLERHGVA-VFSS----NY 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 90 TKYREASVEVMEIMSHFAV-IERASIDEAYIDLTnavqerleklqgqpvsadllpstyieglprgpavedtvekedirkq 168
Cdd:cd01700 73 ALYGDMSRRIMSILERFSPdVEVYSIDESFLDLT---------------------------------------------- 106
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 169 gllqwlGSLEKDDPTSpdlrltvgavIVEEMRAAIERKTGFQCSAGISHNKVLAKLACGLNKPNRQT-----LVSHGSVP 243
Cdd:cd01700 107 ------GSLRFGDLEE----------LARKIRRRILQETGIPVTVGIGPTKTLAKLANDLAKKKNPYggvvdLTDEEVRD 170
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 244 QLFSQMPIRKI----RSLGGKLGAsvidvLGVEYMGDLTQFTEAQLQSHFGeKNGSWLYAMCRGI---EHEPVKPRQlpK 316
Cdd:cd01700 171 KLLKILPVGDVwgigRRTAKKLNA-----MGIHTAGDLAQADPDLLRKKFG-VVGERLVRELNGIdclPLEEYPPPK--K 242
|
....*....
gi 157817057 317 TIGCSKNFP 325
Cdd:cd01700 243 SIGSSRSFG 251
|
|
| PRK02794 |
PRK02794 |
DNA polymerase IV; Provisional |
6-318 |
2.71e-26 |
|
DNA polymerase IV; Provisional
Pssm-ID: 179473 [Multi-domain] Cd Length: 419 Bit Score: 111.95 E-value: 2.71e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 6 DLVVALVDMDCFFVQVEQRQNPHLRNKPCAVvqykswkGGG----IIAVSYEARAFGVTRNMWADDAKKLCPDlllAQVR 81
Cdd:PRK02794 35 TLSIAHIDCDAFYASVEKRDNPELRDKPVII-------GGGkrgvVSTACYIARIHGVRSAMPMFKALKLCPD---AVVI 104
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 82 esrgKANLTKYREASVEVMEIMSHFA-VIERASIDEAYIDLTNAvqerlEKLQGQPvsadllpstyieglprgPAVedtv 160
Cdd:PRK02794 105 ----KPDMEKYVRVGREVRAMMQALTpLVEPLSIDEAFLDLSGT-----ERLHGAP-----------------PAV---- 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 161 ekedirkqgllqwlgSLekddptspdLRLTvgaviveemrAAIERKTGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHG 240
Cdd:PRK02794 155 ---------------VL---------ARFA----------RRVEREIGITVSVGLSYNKFLAKIASDLDKPRGFSVIGRA 200
|
250 260 270 280 290 300 310
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 157817057 241 SVPQLFSQMPIRKIRSLGGKLGASVIDvLGVEYMGDLTQFTEAQLQSHFGEkNGSWLYAMCRGIEHEPVKPRQLPKTI 318
Cdd:PRK02794 201 EALAFLAPKPVGIIWGVGPATAARLAR-DGIRTIGDLQRADEADLMRRFGS-MGLRLWRLARGIDDRKVSPDREAKSV 276
|
|
| PRK03858 |
PRK03858 |
DNA polymerase IV; Validated |
13-319 |
3.35e-26 |
|
DNA polymerase IV; Validated
Pssm-ID: 179663 [Multi-domain] Cd Length: 396 Bit Score: 111.23 E-value: 3.35e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 13 DMDCFFVQVEQRQNPHLRNKPCAVvqykswkGGGII-AVSYEARAFGVTRNMWADDAKKLCPDlllAQVRESRGKAnltk 91
Cdd:PRK03858 10 DLDSFYASVEQRDDPALRGRPVIV-------GGGVVlAASYEAKAYGVRTAMGGRQARRLCPQ---AVVVPPRMSA---- 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 92 YREASVEVMEIMSHFA-VIERASIDEAYIDLTNavqerLEKLQGQPVSadllpstyieglprgpavedtvekedirkqgl 170
Cdd:PRK03858 76 YSRASKAVFEVFRDTTpLVEGLSIDEAFLDVGG-----LRRISGTPVQ-------------------------------- 118
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 171 lqwlgslekddptspdlrltvgavIVEEMRAAIERKTGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQLFSQMP 250
Cdd:PRK03858 119 ------------------------IAARLRRRVREEVGLPITVGVARTKFLAKVASQVAKPDGLLVVPPDRELAFLHPLP 174
|
250 260 270 280 290 300
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 157817057 251 IRKIRSLGGKLgASVIDVLGVEYMGDLTQFTEAQLQSHFGEKNGSWLYAMCRGIEHEPVKPRQLPKTIG 319
Cdd:PRK03858 175 VRRLWGVGPVT-AAKLRAHGITTVGDVAELPESALVSLLGPAAGRHLHALAHNRDPRRVETGRRRRSVG 242
|
|
| PRK03352 |
PRK03352 |
DNA polymerase IV; Validated |
12-370 |
2.08e-21 |
|
DNA polymerase IV; Validated
Pssm-ID: 179564 [Multi-domain] Cd Length: 346 Bit Score: 96.24 E-value: 2.08e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 12 VDMDCFFVQVEQRQNPHLRNKPCAVvqykswkGG--------GIIA-VSYEARAFGVTRNMWADDAKKLCPD-LLLAQVR 81
Cdd:PRK03352 10 VDLDQFIAAVELLRRPELAGLPVIV-------GGngdpteprKVVTcASYEARAFGVRAGMPLRTAARRCPDaVFLPSDP 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 82 ESrgkanltkYREASVEVMEIM-SHFAVIERASIDEAYIdltnavqerleklqgqpvsadllpstyieglprgpavedtv 160
Cdd:PRK03352 83 AA--------YDAASEEVMATLrDLGVPVEVWGWDEAFL----------------------------------------- 113
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 161 ekedirkqgllqwlgSLEKDDPTSpdlrltvgavIVEEMRAAIERKTGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHG 240
Cdd:PRK03352 114 ---------------GVDTDDPEA----------LAEEIRAAVLERTGLSCSVGIGDNKLRAKIATGFAKPAGVFRLTDA 168
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 241 SVPQLFSQMPIRKIRSLGGKlGASVIDVLGVEYMGDLTQFTEAQLQSHFGEKNGSWLYAMCRGIEHEPV--KPRQlPKTI 318
Cdd:PRK03352 169 NWMAVMGDRPTDALWGVGPK-TAKRLAALGITTVADLAAADPAELAATFGPTTGPWLLLLARGGGDTEVsaEPWV-PRSR 246
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|..
gi 157817057 319 GCSKNFPgkTALATREQVQwwllQLALELEERLTKDRTDNGRVATQLVVSIR 370
Cdd:PRK03352 247 SREVTFP--QDLTDRAEVE----SAVRELARRVLDEVVAEGRPVTRVAVKVR 292
|
|
| zf_UBZ |
pfam18439 |
Ubiquitin-Binding Zinc Finger; This is ubiquitin-binding zinc finger (UBZ) domain found in DNA ... |
608-638 |
2.50e-11 |
|
Ubiquitin-Binding Zinc Finger; This is ubiquitin-binding zinc finger (UBZ) domain found in DNA polymerase eta (EC:2.7.7.7). It is important in the recruitment of the polymerase to the stalled replication machinery in translesion synthesis. The UBZ domain adopts a classical C2H2 zinc-finger structure characterized by a beta-beta-alpha fold.
Pssm-ID: 465769 Cd Length: 32 Bit Score: 58.68 E-value: 2.50e-11
10 20 30
....*....|....*....|....*....|.
gi 157817057 608 DQVLCEKCDSLVPVWDMPEHLDYHFALELQK 638
Cdd:pfam18439 1 DTYVCPRCGKEIPESELPEHEDWHFAKDLQR 31
|
|
| PTZ00205 |
PTZ00205 |
DNA polymerase kappa; Provisional |
12-277 |
8.86e-08 |
|
DNA polymerase kappa; Provisional
Pssm-ID: 140232 [Multi-domain] Cd Length: 571 Bit Score: 55.41 E-value: 8.86e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 12 VDMDCFFVQVEQRQNPHLRNKPCAVVQYKSwkgggIIAVSYEARAFGVTRNMWADDAKKLCPDLLLAqvresrgKANLTK 91
Cdd:PTZ00205 138 LDMDMFYAAVEIKKHPEYAAIPLAIGTMTM-----LQTANYVARGRGIRQGMPGFLALKICPNLLIL-------PPDFDA 205
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 92 YREASVEVMEIMSHFavierasiDEAYIDLTnavqerLEKLQgqpvsadLLPSTYIEGLprgpavEDTVEKEDIrkqgll 171
Cdd:PTZ00205 206 YNEESNTVRRIVAEY--------DPNYISFG------LDELT-------LEVSAYIERF------EGTKTAEDV------ 252
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 172 qwlgslekddptspdlrltvgaviVEEMRAAIERKTGFQCSAGISHNKVLAKLACGLNKPNRQ---TLVSHGSVPQLFSQ 248
Cdd:PTZ00205 253 ------------------------ASELRVRVFGETKLTASAGIGPTAALAKIASNINKPNGQhdlNLHTRGDVMTYVRD 308
|
250 260
....*....|....*....|....*....
gi 157817057 249 MPIRKIRSLgGKLGASVIDVLGVEYMGDL 277
Cdd:PTZ00205 309 LGLRSVPGV-GKVTEALLKGLGITTLSDI 336
|
|
| PolY_like |
cd03468 |
DNA Polymerase Y-family; Y-family DNA polymerases are a specialized subset of polymerases that ... |
18-316 |
1.88e-04 |
|
DNA Polymerase Y-family; Y-family DNA polymerases are a specialized subset of polymerases that facilitate translesion synthesis (TLS), a process that allows the bypass of a variety of DNA lesions. Unlike replicative polymerases, TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. The active sites of TLS polymerases are large and flexible to allow the accomodation of distorted bases. Expression of Y-family polymerases is often induced by DNA damage and is believed to be highly regulated. TLS is likely induced by the monoubiquitination of the replication clamp PCNA, which provides a scaffold for TLS polymerases to bind in order to access the lesion. Because of their high error rates, TLS polymerases are potential targets for cancer treatment and prevention.
Pssm-ID: 176458 [Multi-domain] Cd Length: 335 Bit Score: 44.29 E-value: 1.88e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 18 FVQVEQRQNPHLRNKPCAVVQYKswKGGGIIAVSYEARAFGVTRNMWADDAKKLCPDLLLAQVRESRGKANLTKYREAsv 97
Cdd:cd03468 9 PLDALLRNRPADDEAPLAVVERK--KAGRILACNAAARAAGVRPGMPLAEALALCPNLQVVEYDPEADARALQELALW-- 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 98 evmeIMSHFAVIERASIDEAYIDLTNAvqerleklqgqpvsadllpstyieglprgpavedtvekedirkqgllqwlgsl 177
Cdd:cd03468 85 ----LLRFTPLVALDGPDGLLLDVTGC----------------------------------------------------- 107
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 178 ekddptspdLRLTVGAVIVEEMRAAIERKTGFQCSAGISHNKVLAKLACGLNKP---NRQTlvSHGSVPQLFSQMPIRKI 254
Cdd:cd03468 108 ---------LHLFGGEDALAASLRAALATLGLSARAGIADTPGAAWLLARAGGGrgvLRRE--ALAAALVLLAPLPVAAL 176
|
250 260 270 280 290 300
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 157817057 255 RsLGGKLGASVIDvLGVEYMGDLTQFTEAQLQSHFGeKNGSWLYAMCRGIEHEPVKPRQLPK 316
Cdd:cd03468 177 R-LPPETVELLAR-LGLRTLGDLAALPRAELARRFG-LALLLRLDQAYGRDPEPLLFSPPPP 235
|
|
| umuC |
PRK03609 |
translesion error-prone DNA polymerase V subunit UmuC; |
10-327 |
2.06e-04 |
|
translesion error-prone DNA polymerase V subunit UmuC;
Pssm-ID: 179607 [Multi-domain] Cd Length: 422 Bit Score: 44.37 E-value: 2.06e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 10 ALVDMDCFFVQVEQRQNPHLRNKPCAVVqykSWKGGGIIAVSYEARAFGVtrnmwaddaKKLCPDLLLAQVRESRG---- 85
Cdd:PRK03609 3 ALCDVNSFYASCETVFRPDLRGKPVVVL---SNNDGCVIARSAEAKALGI---------KMGDPWFKQKDLFRRCGvvcf 70
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 86 KANLTKYREASVEVMEIMSHFA-VIERASIDEAYIDLTNavqerleklqgqpvsadllpstyieglprgpaVEDTVEKED 164
Cdd:PRK03609 71 SSNYELYADMSNRVMSTLEELSpRVEIYSIDEAFCDLTG--------------------------------VRNCRDLTD 118
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 165 IRKQgllqwlgslekddptspdlrltvgaviveeMRAAIERKTGFQCSAGISHNKVLAKLACGLNKP-NRQT-----LVS 238
Cdd:PRK03609 119 FGRE------------------------------IRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKwQRQTggvvdLSN 168
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157817057 239 HGSVPQLFSQMPIRKIRSLGGKLgASVIDVLGVEYMGDLTQFTEAQLQSHFG--------EKNGSwlyaMCRGIEHEPVK 310
Cdd:PRK03609 169 LERQRKLLSLQPVEEVWGVGRRI-SKKLNAMGIKTALDLADTNIRFIRKHFNvvlertvrELRGE----PCLSLEEFAPT 243
|
330
....*....|....*..
gi 157817057 311 PRQlpktIGCSKNFPGK 327
Cdd:PRK03609 244 KQE----IVCSRSFGER 256
|
|
| |
