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Conserved domains on  [gi|157819051|ref|NP_001102302|]
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rRNA methyltransferase 1, mitochondrial [Rattus norvegicus]

Protein Classification

TrmH family RNA methyltransferase( domain architecture ID 13742638)

TrmH family RNA methyltransferase catalyzes the methylation of rRNA or tRNA at specific sites in a S-adenosyl-L-methionine (SAM)-dependent manner

CATH:  3.40.1280.10
EC:  2.1.1.-
Gene Ontology:  GO:0008173|GO:1904047|GO:0003723|GO:0032259
PubMed:  11763972|17338813|30457841|18029778
SCOP:  3001122

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
SpoU-like_MRM1 cd18105
SAM-dependent rRNA methylase related to MRM1; MRM1 catalyzes the methylation of 2'-O-ribose ...
 144-301 1.05e-75

SAM-dependent rRNA methylase related to MRM1; MRM1 catalyzes the methylation of 2'-O-ribose residues G1145 to GmG residue of the mitochondrial 16S rRNA. MRM1 is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


:

Pssm-ID: 349978  Cd Length: 158  Bit Score: 228.94  E-value: 1.05e-75
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051 144 LWLVLEGIQDPRNLGAVMRSAYFLGVDRVITSRRNSCPLTPVVSKASAGAMEVMDVFASPDLASFLQAKAQQGWLVVGTV 223
Cdd:cd18105    1 LWLLLDEITDPQNLGAILRSAYFLGVDGVVVSSKNSAPLSPVVSKASAGALELMPIYSVDNLASFLKSSKENGWKVVGTS 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 157819051 224 GCPGPEiSQSSKVPVTSCLEFIWDRPTLLVLGNEGSGLSQGVFATCQLLLTILPGRHLP-PGLESLNVSVATGILLHSI 301
Cdd:cd18105   81 APPSAS-SDKKTIPANELSELLLEKPTILVLGNEGRGLRTNIKNLCDYLVSIPGGRSAEnPGVDSLNVSVAAGILLHSL 158
SpoU_sub_bind pfam08032
RNA 2'-O ribose methyltransferase substrate binding; This domain is a RNA 2'-O ribose ...
 50-125 3.27e-13

RNA 2'-O ribose methyltransferase substrate binding; This domain is a RNA 2'-O ribose methyltransferase substrate binding domain.


:

Pssm-ID: 429793 [Multi-domain]  Cd Length: 74  Bit Score: 63.80  E-value: 3.27e-13
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 157819051   50 LFGVSPCLLALRAARRhVARLLLQAGKAGLQGEraELLRVAEARGIPVLRPRRQKLDALCGYQVHQGVCMEVSRLR 125
Cdd:pfam08032   2 IYGRHAVLEALKSGPE-IKKLYLTEGRRDPRLK--ELLKLAKERGIKVEEVDRKKLDKLSGTANHQGVVAEVKPYE 74
 
Name Accession Description Interval E-value
SpoU-like_MRM1 cd18105
SAM-dependent rRNA methylase related to MRM1; MRM1 catalyzes the methylation of 2'-O-ribose ...
 144-301 1.05e-75

SAM-dependent rRNA methylase related to MRM1; MRM1 catalyzes the methylation of 2'-O-ribose residues G1145 to GmG residue of the mitochondrial 16S rRNA. MRM1 is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349978  Cd Length: 158  Bit Score: 228.94  E-value: 1.05e-75
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051 144 LWLVLEGIQDPRNLGAVMRSAYFLGVDRVITSRRNSCPLTPVVSKASAGAMEVMDVFASPDLASFLQAKAQQGWLVVGTV 223
Cdd:cd18105    1 LWLLLDEITDPQNLGAILRSAYFLGVDGVVVSSKNSAPLSPVVSKASAGALELMPIYSVDNLASFLKSSKENGWKVVGTS 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 157819051 224 GCPGPEiSQSSKVPVTSCLEFIWDRPTLLVLGNEGSGLSQGVFATCQLLLTILPGRHLP-PGLESLNVSVATGILLHSI 301
Cdd:cd18105   81 APPSAS-SDKKTIPANELSELLLEKPTILVLGNEGRGLRTNIKNLCDYLVSIPGGRSAEnPGVDSLNVSVAAGILLHSL 158
SpoU COG0566
tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA ...
 50-307 5.49e-61

tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA G18 (ribose-2'-O)-methylase SpoU is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440332 [Multi-domain]  Cd Length: 265  Bit Score: 195.41  E-value: 5.49e-61
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051  50 LFGVSPCLLALRAaRRHVARLLLQAGkagLQGERAELLRVAEARGIPVLRPRRQKLDALCGYQVHQGVCMEVSRLRPRPC 129
Cdd:COG0566   29 AEGRHAVEEALKA-GREIEELLVTEG---ALDRLAELLALAKAAGIPVYEVSREVLDKLSGTETHQGVLAVVRPPEYADL 104

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051 130 DEAADTSSGDDpqqLWLVLEGIQDPRNLGAVMRSAYFLGVDRVITSRRNSCPLTPVVSKASAGAMEVMDVFASPDLASFL 209
Cdd:COG0566  105 EDLLAEAGEPP---LLLVLDGVQDPGNLGAILRSAAAFGVDGVILPKRRADPYNPKVVRASAGALFHVPVARVTNLADAL 181

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051 210 QAKAQQGWLVVGTVGcpgpeisqSSKVPVTsclEFIWDRPTLLVLGNEGSGLSQGVFATCQLLLTIlPgrhLPPGLESLN 289
Cdd:COG0566  182 EELKEAGFWVVGTDL--------DGEASLY---EVDLPGPVALVLGNEGKGLSRLVLEACDELVRI-P---MAGGVESLN 246

                        250
                 ....*....|....*...
gi 157819051 290 VSVATGILLHSICSQKKG 307
Cdd:COG0566  247 VAVAAAILLYEAARQRRA 264
rRNA_methyl_3 TIGR00186
rRNA methylase, putative, group 3; this is part of the trmH (spoU) family of rRNA methylases ...
 47-305 3.20e-45

rRNA methylase, putative, group 3; this is part of the trmH (spoU) family of rRNA methylases [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 129290 [Multi-domain]  Cd Length: 237  Bit Score: 153.65  E-value: 3.20e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051   47 LERLFGVSPCLLALRAARRhvarllLQAGKAGLQGERAELLRVAEARGIPVLRPRRQKLDALCGYQVHQGVCMEVSRLRP 126
Cdd:TIGR00186   1 QEYLYGKNAVLEALLNQQR------VFILKGLESKRLKKLIQLAKKQGINIQLVDRQKLDQLTKGGNHQGIAAKVKPILY 74

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051  127 RPCDEAADTS-SGDDPqqLWLVLEGIQDPRNLGAVMRSAYFLGVDRVITSRRNSCPLTPVVSKASAGAMEVMDVFASPDL 205
Cdd:TIGR00186  75 KDLNDLYKTAkSKKQP--FLLILDEITDPHNLGAILRTAEAFGVDGVILPKRRSAPLNSTVVKTSSGAVEYVPLARVTNL 152

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051  206 ASFLQAKAQQGWLVVGTVGCPGPEISQSSKvpvtsclefiwDRPTLLVLGNEGSGLSQGVFATCQLLLTIlPGRhlpPGL 285
Cdd:TIGR00186 153 SRTITKLKESGFWTVGTDLDAQDTLYQVKL-----------TKPLALVVGNEGEGVSRLIKENCDFLIKI-PMA---GKV 217

                         250       260
                  ....*....|....*....|
gi 157819051  286 ESLNVSVATGILLHSICSQK 305
Cdd:TIGR00186 218 DSLNVSVAAGILLFEIKRQR 237
SpoU_methylase pfam00588
SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.
 146-299 1.16e-36

SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.


Pssm-ID: 425768  Cd Length: 142  Bit Score: 128.42  E-value: 1.16e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051  146 LVLEGIQDPRNLGAVMRSAYFLGVDRVITSRRNSC-PLTPVVSKASAGAMEVMDVFASPDLASFLQAKAQQGWLVVGTvg 224
Cdd:pfam00588   4 VVLDNIQDPGNLGAIIRTAAAFGADGLILVEPRSAdPYNPKAIRASAGALFHLPLVVVDNLEEALKDLKEAGYAVVAT-- 81

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 157819051  225 cpgpeiSQSSKvpvtSCLEFIWDRPTLLVLGNEGSGLSQGVFATCQLLLTIlPGRhlpPGLESLNVSVATGILLH 299
Cdd:pfam00588  82 ------SLGGK----SLYEIDFPKKVALVFGNEGTGLSNEVLELCDRLVRI-PMS---GDVESLNVAVAAAIALY 142
PRK11181 PRK11181
23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB;
86-305 1.72e-28

23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB;


Pssm-ID: 183021 [Multi-domain]  Cd Length: 244  Bit Score: 110.06  E-value: 1.72e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051  86 LLRVAEARGIPVLRPRRQKLDALCGYQVHQGVcmeVSRLRP-RPCDEA---ADTSSGDDPqqLWLVLEGIQDPRNLGAVM 161
Cdd:PRK11181  39 LINELEAQGIVIQLANRQTLDEKAEGAVHQGI---IARVKPgRQLQENdlpDLLASLEQP--FLLILDGVTDPHNLGACL 113

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051 162 RSAYFLGVDRVITSRRNSCPLTPVVSKASAGAMEVMDVFASPDLASFLQAKAQQGWLVVGTVGCPGPEISQSSkvpVTSc 241
Cdd:PRK11181 114 RSADAAGVHAVIVPKDRSAQLNATAKKVACGAAETVPLIRVTNLARTMRMLQEKNIWIVGTAGEADHTLYQSK---LTG- 189

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 157819051 242 lefiwdrPTLLVLGNEGSGLSQGVFATCQLLLTI-LPGrhlppGLESLNVSVATGILLHSICSQK 305
Cdd:PRK11181 190 -------PLALVMGAEGEGMRRLTREHCDELISIpMAG-----SVSSLNVSVATGICLFEAVRQR 242
SpoU_sub_bind pfam08032
RNA 2'-O ribose methyltransferase substrate binding; This domain is a RNA 2'-O ribose ...
 50-125 3.27e-13

RNA 2'-O ribose methyltransferase substrate binding; This domain is a RNA 2'-O ribose methyltransferase substrate binding domain.


Pssm-ID: 429793 [Multi-domain]  Cd Length: 74  Bit Score: 63.80  E-value: 3.27e-13
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 157819051   50 LFGVSPCLLALRAARRhVARLLLQAGKAGLQGEraELLRVAEARGIPVLRPRRQKLDALCGYQVHQGVCMEVSRLR 125
Cdd:pfam08032   2 IYGRHAVLEALKSGPE-IKKLYLTEGRRDPRLK--ELLKLAKERGIKVEEVDRKKLDKLSGTANHQGVVAEVKPYE 74
SpoU_sub_bind smart00967
RNA 2'-O ribose methyltransferase substrate binding; This domain is a RNA 2'-O ribose ...
 50-122 1.05e-10

RNA 2'-O ribose methyltransferase substrate binding; This domain is a RNA 2'-O ribose methyltransferase substrate binding domain.


Pssm-ID: 214943 [Multi-domain]  Cd Length: 70  Bit Score: 56.78  E-value: 1.05e-10
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 157819051    50 LFGVSPCLLALRAARRhVARLLLQAGkagLQGERAELLRVAEARGIPVLRPRRQKLDALCGYQVHQGVCMEVS 122
Cdd:smart00967   1 IEGRHAVEEALKSGRR-IKELYLTEG---RAERLKELLKLAKERGIKVIEVSKKELDKLSGTANHQGVVAVVK 69
 
Name Accession Description Interval E-value
SpoU-like_MRM1 cd18105
SAM-dependent rRNA methylase related to MRM1; MRM1 catalyzes the methylation of 2'-O-ribose ...
 144-301 1.05e-75

SAM-dependent rRNA methylase related to MRM1; MRM1 catalyzes the methylation of 2'-O-ribose residues G1145 to GmG residue of the mitochondrial 16S rRNA. MRM1 is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349978  Cd Length: 158  Bit Score: 228.94  E-value: 1.05e-75
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051 144 LWLVLEGIQDPRNLGAVMRSAYFLGVDRVITSRRNSCPLTPVVSKASAGAMEVMDVFASPDLASFLQAKAQQGWLVVGTV 223
Cdd:cd18105    1 LWLLLDEITDPQNLGAILRSAYFLGVDGVVVSSKNSAPLSPVVSKASAGALELMPIYSVDNLASFLKSSKENGWKVVGTS 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 157819051 224 GCPGPEiSQSSKVPVTSCLEFIWDRPTLLVLGNEGSGLSQGVFATCQLLLTILPGRHLP-PGLESLNVSVATGILLHSI 301
Cdd:cd18105   81 APPSAS-SDKKTIPANELSELLLEKPTILVLGNEGRGLRTNIKNLCDYLVSIPGGRSAEnPGVDSLNVSVAAGILLHSL 158
SpoU COG0566
tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA ...
 50-307 5.49e-61

tRNA G18 (ribose-2'-O)-methylase SpoU [Translation, ribosomal structure and biogenesis]; tRNA G18 (ribose-2'-O)-methylase SpoU is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440332 [Multi-domain]  Cd Length: 265  Bit Score: 195.41  E-value: 5.49e-61
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051  50 LFGVSPCLLALRAaRRHVARLLLQAGkagLQGERAELLRVAEARGIPVLRPRRQKLDALCGYQVHQGVCMEVSRLRPRPC 129
Cdd:COG0566   29 AEGRHAVEEALKA-GREIEELLVTEG---ALDRLAELLALAKAAGIPVYEVSREVLDKLSGTETHQGVLAVVRPPEYADL 104

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051 130 DEAADTSSGDDpqqLWLVLEGIQDPRNLGAVMRSAYFLGVDRVITSRRNSCPLTPVVSKASAGAMEVMDVFASPDLASFL 209
Cdd:COG0566  105 EDLLAEAGEPP---LLLVLDGVQDPGNLGAILRSAAAFGVDGVILPKRRADPYNPKVVRASAGALFHVPVARVTNLADAL 181

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051 210 QAKAQQGWLVVGTVGcpgpeisqSSKVPVTsclEFIWDRPTLLVLGNEGSGLSQGVFATCQLLLTIlPgrhLPPGLESLN 289
Cdd:COG0566  182 EELKEAGFWVVGTDL--------DGEASLY---EVDLPGPVALVLGNEGKGLSRLVLEACDELVRI-P---MAGGVESLN 246

                        250
                 ....*....|....*...
gi 157819051 290 VSVATGILLHSICSQKKG 307
Cdd:COG0566  247 VAVAAAILLYEAARQRRA 264
rRNA_methyl_3 TIGR00186
rRNA methylase, putative, group 3; this is part of the trmH (spoU) family of rRNA methylases ...
 47-305 3.20e-45

rRNA methylase, putative, group 3; this is part of the trmH (spoU) family of rRNA methylases [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 129290 [Multi-domain]  Cd Length: 237  Bit Score: 153.65  E-value: 3.20e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051   47 LERLFGVSPCLLALRAARRhvarllLQAGKAGLQGERAELLRVAEARGIPVLRPRRQKLDALCGYQVHQGVCMEVSRLRP 126
Cdd:TIGR00186   1 QEYLYGKNAVLEALLNQQR------VFILKGLESKRLKKLIQLAKKQGINIQLVDRQKLDQLTKGGNHQGIAAKVKPILY 74

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051  127 RPCDEAADTS-SGDDPqqLWLVLEGIQDPRNLGAVMRSAYFLGVDRVITSRRNSCPLTPVVSKASAGAMEVMDVFASPDL 205
Cdd:TIGR00186  75 KDLNDLYKTAkSKKQP--FLLILDEITDPHNLGAILRTAEAFGVDGVILPKRRSAPLNSTVVKTSSGAVEYVPLARVTNL 152

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051  206 ASFLQAKAQQGWLVVGTVGCPGPEISQSSKvpvtsclefiwDRPTLLVLGNEGSGLSQGVFATCQLLLTIlPGRhlpPGL 285
Cdd:TIGR00186 153 SRTITKLKESGFWTVGTDLDAQDTLYQVKL-----------TKPLALVVGNEGEGVSRLIKENCDFLIKI-PMA---GKV 217

                         250       260
                  ....*....|....*....|
gi 157819051  286 ESLNVSVATGILLHSICSQK 305
Cdd:TIGR00186 218 DSLNVSVAAGILLFEIKRQR 237
SpoU-like_RlmB cd18103
SAM-dependent rRNA methylase related to RlmB; 23S rRNA-M2G2251-MTase RlmB catalyzes the ...
 144-301 1.16e-44

SAM-dependent rRNA methylase related to RlmB; 23S rRNA-M2G2251-MTase RlmB catalyzes the methylation of guanosine 2251, a modification conserved in the peptidyltransferase domain of 23S rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349976  Cd Length: 143  Bit Score: 149.07  E-value: 1.16e-44
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051 144 LWLVLEGIQDPRNLGAVMRSAYFLGVDRVITSRRNSCPLTPVVSKASAGAMEVMDVFASPDLASFLQAKAQQGWLVVGTv 223
Cdd:cd18103    1 LLLILDGITDPHNLGAIIRSAEAFGVDGVIIPKRRSAPLTPTVAKASAGALEHLPIARVTNLARALEELKEAGFWIYGA- 79

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 157819051 224 gcpgpeiSQSSKVPVTsclEFIWDRPTLLVLGNEGSGLSQGVFATCQLLLTIlPgrhLPPGLESLNVSVATGILLHSI 301
Cdd:cd18103   80 -------DGEAEKSLY---DVDLTGPLALVLGSEGKGLRRLVLKNCDFLVKI-P---MAGKVDSLNVSVAAGIILYEI 143
SpoU-like_rRNA-MTase cd18095
SAM-dependent rRNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 144-301 3.74e-43

SAM-dependent rRNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349968  Cd Length: 143  Bit Score: 145.26  E-value: 3.74e-43
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051 144 LWLVLEGIQDPRNLGAVMRSAYFLGVDRVITSRRNSCPLTPVVSKASAGAMEVMDVFASPDLASFLQAKAQQGWLVVGTV 223
Cdd:cd18095    1 LVLVLDGVQDPGNLGTIIRTAAAFGVDGVILSKGTVDPYNPKVVRASMGALFRLPIIRVDDLEELLEELKEAGYKVVAAD 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 157819051 224 GCPGpeisqsskvpvTSCLEFIWDRPTLLVLGNEGSGLSQGVFATCQLLLTIlPGrhlPPGLESLNVSVATGILLHSI 301
Cdd:cd18095   81 LDGG-----------SSLYDLDLPKPVALVLGNEGNGLSDELLELCDKRVTI-PM---SGGVESLNVAVAAAILLYEL 143
SpoU_methylase pfam00588
SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.
 146-299 1.16e-36

SpoU rRNA Methylase family; This family of proteins probably use S-AdoMet.


Pssm-ID: 425768  Cd Length: 142  Bit Score: 128.42  E-value: 1.16e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051  146 LVLEGIQDPRNLGAVMRSAYFLGVDRVITSRRNSC-PLTPVVSKASAGAMEVMDVFASPDLASFLQAKAQQGWLVVGTvg 224
Cdd:pfam00588   4 VVLDNIQDPGNLGAIIRTAAAFGADGLILVEPRSAdPYNPKAIRASAGALFHLPLVVVDNLEEALKDLKEAGYAVVAT-- 81

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 157819051  225 cpgpeiSQSSKvpvtSCLEFIWDRPTLLVLGNEGSGLSQGVFATCQLLLTIlPGRhlpPGLESLNVSVATGILLH 299
Cdd:pfam00588  82 ------SLGGK----SLYEIDFPKKVALVFGNEGTGLSNEVLELCDRLVRI-PMS---GDVESLNVAVAAAIALY 142
PRK11181 PRK11181
23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB;
86-305 1.72e-28

23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB;


Pssm-ID: 183021 [Multi-domain]  Cd Length: 244  Bit Score: 110.06  E-value: 1.72e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051  86 LLRVAEARGIPVLRPRRQKLDALCGYQVHQGVcmeVSRLRP-RPCDEA---ADTSSGDDPqqLWLVLEGIQDPRNLGAVM 161
Cdd:PRK11181  39 LINELEAQGIVIQLANRQTLDEKAEGAVHQGI---IARVKPgRQLQENdlpDLLASLEQP--FLLILDGVTDPHNLGACL 113

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051 162 RSAYFLGVDRVITSRRNSCPLTPVVSKASAGAMEVMDVFASPDLASFLQAKAQQGWLVVGTVGCPGPEISQSSkvpVTSc 241
Cdd:PRK11181 114 RSADAAGVHAVIVPKDRSAQLNATAKKVACGAAETVPLIRVTNLARTMRMLQEKNIWIVGTAGEADHTLYQSK---LTG- 189

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 157819051 242 lefiwdrPTLLVLGNEGSGLSQGVFATCQLLLTI-LPGrhlppGLESLNVSVATGILLHSICSQK 305
Cdd:PRK11181 190 -------PLALVMGAEGEGMRRLTREHCDELISIpMAG-----SVSSLNVSVATGICLFEAVRQR 242
SpoU-like_TrmH cd18092
SAM-dependent tRNA methylase related to TrmH; TrmH catalyzes the transfer of the methyl group ...
 146-304 2.37e-22

SAM-dependent tRNA methylase related to TrmH; TrmH catalyzes the transfer of the methyl group from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of the ribose of the universally conserved guanosine 18 (G18) position in tRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349965  Cd Length: 162  Bit Score: 91.37  E-value: 2.37e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051 146 LVLEGIQDPRNLGAVMRSAYFLGVDRV-ITSRRNScplTPVVSKASAGAMEVMDVFASPDLASFLQAKAQQGWLVVGTvg 224
Cdd:cd18092   19 VVLENVHDPHNASAVLRTCDAFGVQDVhVIEPKNK---FKKNRGVAMGADKWLDVHRHDSTEECLAALKEQGYRIVAT-- 93

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051 225 CPGPEisqssKVPVTsclEFIWDRPTLLVLGNEGSGLSQGVFATCQLLLTIlpgrhlpP--GL-ESLNVSVATGILLHSI 301
Cdd:cd18092   94 SLSPD-----AVPLD---ELDYTRPTALVFGNEKDGVSEEALAAADERVYI-------PmyGFvESLNVSVAAAIILYEL 158


                 ...
gi 157819051 302 CSQ 304
Cdd:cd18092  159 QRQ 161
SpoU-like_family cd18082
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 146-301 2.36e-21

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349955  Cd Length: 145  Bit Score: 88.05  E-value: 2.36e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051 146 LVLEGIQDPRNLGAVMRSAYFLGVDRVITSRRNSCPLTPVVSKASAGAMEVMDVFASPDLASFLQAKAQQGWLVVGTvgc 225
Cdd:cd18082    2 LVLDNIRSPGNIGSIFRTADAFGVEKIYLVGPTADIFDPKAKRTSRGTHFSVPYERVDNFEEYLKSLKEEGYPLIGL--- 78

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 157819051 226 pgpEISQSSKvPVTSClEFIWDRPTLLVLGNEGSGLSQGVFATCQLLLTIlPGRhlpPGLESLNVSVATGILLHSI 301
Cdd:cd18082   79 ---ELTDGSI-PLSEF-KFKKPKKIALIFGNERSGLSPELLALCDYTVHI-PMS---GKNSSLNVSVATGIALYEF 145
SpoU-like_RNA-MTase cd18109
SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 146-298 1.61e-19

SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349982  Cd Length: 141  Bit Score: 82.97  E-value: 1.61e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051 146 LVLEGIQDPRNLGAVMRSAYFLGVDRVITSRRNSCPLTPVVSKASAGAMEVMDVFASpDLASFLQAkAQQGWLVVGTVgC 225
Cdd:cd18109    3 LALDGIQDPGNLGTIIRTADWFGIRQIICSPDTVDPYNPKVVQASMGSIFRVQIHYT-DLPALLAE-LKKAFPVYGAD-L 79

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 157819051 226 PGPEISqsskvpvtsclEFIWDRPTLLVLGNEGSGLSQGVFATCQLLLTIlPGRhlPPGLESLNVSVATGILL 298
Cdd:cd18109   80 DGENLY-----------EFTLPEKGILVMGNEGHGISPEVLKLLTHRLTI-PRY--GKGAESLNVAVATAILL 138
SpoU-like cd18097
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 144-298 2.12e-18

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349970  Cd Length: 144  Bit Score: 80.30  E-value: 2.12e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051 144 LWLVLEGIQDPRNLGAVMRSAYFLGVDRVITSRRNSCPLTPVVSKASAGAMEVMDVFASPDLASFLQAKAQQGWLVVGTv 223
Cdd:cd18097    1 LVVVLDNLRSAHNVGSIFRTADAAGVEKIYLCGYTPTPPHPKVAKTALGAEESVPWEHFEDTLEAVRALKARGYTIIAL- 79

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 157819051 224 gcpgpEISQSSkVPVTsclEFIWDRPTLLVLGNEGSGLSQGVFATCQLLLTI-LPGRHlppglESLNVSVATGILL 298
Cdd:cd18097   80 -----ETTENS-VPLH---EARLPRPLALVLGNEVRGVSPEVLALCDAVVEIpMFGRK-----NSLNVAVAAAVAL 141
PRK10864 PRK10864
putative methyltransferase; Provisional
 110-298 1.41e-16

putative methyltransferase; Provisional


Pssm-ID: 236779 [Multi-domain]  Cd Length: 346  Bit Score: 79.05  E-value: 1.41e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051 110 GYQVHQGVCMEVSRlrpRPCDEAADTSSGDDPQQLWLVLEGIQDPRNLGAVMRSAYFLGVDRVITSRRNSCPLTPVVSKA 189
Cdd:PRK10864 168 GTEHHGGVCFLIKK---RNGTDVQQWLAQAGAQDCVLALEDVGNPHNLGGIMRSCAHFGVKGVVVQDAALLESGAAIRTA 244

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051 190 SAGAMEVMDVFASpDLASFLQAKAQQGWLVVGTVGCPGPEISQsSKVPvtsclefiwdRPTLLVLGNEGSGLSQGVFATC 269
Cdd:PRK10864 245 EGGAEHVQPITGD-SFVDVLDDFRQAGYTIVTTSSHKGTPLFK-ASLP----------AKMVLVLGQEYDGLSDAARQQG 312

                        170       180
                 ....*....|....*....|....*....
gi 157819051 270 QLLLTIlPGRHLppgLESLNVSVATGILL 298
Cdd:PRK10864 313 DLSVSI-DGTGN---VESLNVSVATGVLL 337
SpoU_sub_bind pfam08032
RNA 2'-O ribose methyltransferase substrate binding; This domain is a RNA 2'-O ribose ...
 50-125 3.27e-13

RNA 2'-O ribose methyltransferase substrate binding; This domain is a RNA 2'-O ribose methyltransferase substrate binding domain.


Pssm-ID: 429793 [Multi-domain]  Cd Length: 74  Bit Score: 63.80  E-value: 3.27e-13
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 157819051   50 LFGVSPCLLALRAARRhVARLLLQAGKAGLQGEraELLRVAEARGIPVLRPRRQKLDALCGYQVHQGVCMEVSRLR 125
Cdd:pfam08032   2 IYGRHAVLEALKSGPE-IKKLYLTEGRRDPRLK--ELLKLAKERGIKVEEVDRKKLDKLSGTANHQGVVAEVKPYE 74
SpoU-like cd18096
SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase ...
 144-300 2.42e-12

SAM-dependent rRNA or tRNA methylase related to SpoU; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349969  Cd Length: 140  Bit Score: 63.39  E-value: 2.42e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051 144 LWLVLEGIQDPRNLGAVMRSAYFLGVDRV-ITSRRnscPLTPVVSKASAGAMEVMDVFASPDLASFLqakAQQGWLVVGT 222
Cdd:cd18096    1 LYLVLHNVAKRHNIGTIVRSANAFGVKEVhLVGRR---KFNPFGAMGTDRHVKFRHFDTLAEAKEYL---KERGIRLVGV 74

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 157819051 223 vgcpgpEISQSSKvPVTSCLefiWDRPTLLVLGNEGSGLSQGVFATCQLLLTIlpgRHLPPGLESLNVSVATGILLHS 300
Cdd:cd18096   75 ------EIVPGAE-PIETHP---FPGDTAFVLGNEGTGLSDEQLAICDGFVYI---PQYGSGTASLNVSVAASIVLHH 139
SpoU-like_RNA-MTase cd18104
SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes ...
 144-298 3.80e-12

SAM-dependent RNA methylase related to SpoU-TrmH; RNA 2'-O ribose methyltransferase catalyzes the methyltransfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH group of ribose in tRNA or rRNA. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349977  Cd Length: 146  Bit Score: 62.98  E-value: 3.80e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051 144 LWLVLEGIQDPRNLGAVMRSAYFLGVDRVITSRRNSCPLTPVVSKASAGAMEVMDVF--ASPDLASFLQakaQQGWLVVG 221
Cdd:cd18104    1 LILVLEGLEKPGNLGAILRSADAAGADAVILCDPKTDLFNPNVIRASLGAVFTLPVAeaSSEETLAWLK---ENKIRILA 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051 222 TvgCPGPEISqsskvpvtsclefIWD----RPTLLVLGNEGSGLSQGVFATCQLLLTIlP--GRhlppgLESLNVSVATG 295
Cdd:cd18104   78 T--TPKAEKL-------------YYEadlrGPIAIVVGSESEGLSEFWLKAADERVRI-PmaGQ-----ADSLNVSVAAA 136


                 ...
gi 157819051 296 ILL 298
Cdd:cd18104  137 ILL 139
SpoU-like_AviRb cd18107
SAM-dependent rRNA methylase related to AviRb; AviRb from Streptomyces viridochromogenes ...
 144-301 9.86e-12

SAM-dependent rRNA methylase related to AviRb; AviRb from Streptomyces viridochromogenes methylates the 2'-O atom of U2479 of the 23S ribosomal RNA. AviRb is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349980  Cd Length: 148  Bit Score: 62.02  E-value: 9.86e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051 144 LWLVLEGIQDPRNLGAVMRSAYFLGVDRVITSRRNSCPLTPVVSKASAG---AMEVMDVFASPDLASFLQA--KAQQGWL 218
Cdd:cd18107    1 LVVLFDRPTSPGNLGTIIRSADALGAHGLVVTGHAADVYDPKTIRASTGslfSLPIVRVPSNRELEDWVEArkARGTPLQ 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051 219 VVGTvgcpgpeiSQSSKVPVTsclEFIWDRPTLLVLGNEGSGLSQGVFATCQLLLTIlpgrHLPPGLESLNVSVATGILL 298
Cdd:cd18107   81 LVGT--------DEHGDHQIA---DHDFTRPTLLLIGNETAGLSAAWRELCDETVSI----PMAGSASSLNAACAASIFL 145


                 ...
gi 157819051 299 HSI 301
Cdd:cd18107  146 YEA 148
SpoU_sub_bind smart00967
RNA 2'-O ribose methyltransferase substrate binding; This domain is a RNA 2'-O ribose ...
 50-122 1.05e-10

RNA 2'-O ribose methyltransferase substrate binding; This domain is a RNA 2'-O ribose methyltransferase substrate binding domain.


Pssm-ID: 214943 [Multi-domain]  Cd Length: 70  Bit Score: 56.78  E-value: 1.05e-10
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 157819051    50 LFGVSPCLLALRAARRhVARLLLQAGkagLQGERAELLRVAEARGIPVLRPRRQKLDALCGYQVHQGVCMEVS 122
Cdd:smart00967   1 IEGRHAVEEALKSGRR-IKELYLTEG---RAERLKELLKLAKERGIKVIEVSKKELDKLSGTANHQGVVAVVK 69
SpoU-like_TRM3-like cd18091
SAM-dependent tRNA methylase related to TRM3; Yeast tRNA (guanosine(18)-2'-O) ...
 151-299 3.74e-09

SAM-dependent tRNA methylase related to TRM3; Yeast tRNA (guanosine(18)-2'-O)-methyltransferase TRM3 catalyzes the formation of 2'-O-methylguanosine at position 18 (Gm18) in various tRNAs. TRM3 is similar to C-terminal domain of TAR (HIV-1) RNA binding protein 1 (TARBP1), a protein binding to TAR, which functions as a RNA regulatory signal by forming a stable stem-loop structure to which transactivator protein Tat binds. The role of TARBP1 is believed to be to disengage RNA polymerase II from TAR during transcriptional elongation. TRM3 and the C-terminal methyltransferase domain of TARBP1 are members of the SPOUT methyltransferase superfamily.


Pssm-ID: 349964  Cd Length: 145  Bit Score: 54.46  E-value: 3.74e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051 151 IQDPRNLGAVMRSAYFLGVDRVITSRRNSC--PLTPVVSKASAGAMEVMDVfASPDLASFLQAKAQQGWLVVGtvgcpgp 228
Cdd:cd18091    8 IDKPPNLGGLCRTCEIFGVSKLVLPNLAVVedKQFKALSVSAEKWLPIEEV-KPEDLLPYLKEKKQEGYTIVG------- 79

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 157819051 229 eISQSSK-VPVTsclEFIWDRPTLLVLGNEGSGLSQGVFATCQLLLTIlpgrhlpPGL---ESLNVSVATGILLH 299
Cdd:cd18091   80 -LEQTANsVSLH---DFQFPKKTVLVLGNEKEGIPAELLSLLDVCVEI-------PQLgviRSLNVHVSGAIAIY 143
SpoU-like_RNMTL1 cd18106
SAM-dependent rRNA methylase related to RNMTL1; RNMTL1 (also known as HC90, MRM3 and RMTL1) ...
 146-298 5.54e-07

SAM-dependent rRNA methylase related to RNMTL1; RNMTL1 (also known as HC90, MRM3 and RMTL1) catalyzes the methylation of 2'-O-ribose residues G1370 to GmG residue of the mitochondrial 16S rRNA. RNMTL1 is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349979  Cd Length: 148  Bit Score: 48.40  E-value: 5.54e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051 146 LVLEGIQDPRNLGAVMRSAYFLGVDRVITSRRNSCPLTPVVSKASAGA---MEVMDVFASPDLASFLQAKAQqgwLVVGt 222
Cdd:cd18106    3 VICDNIRDPGNLGSILRSAAAAGCSKVLLTKGCVDPWEPKVLRAGAGAhfrLPIYGNLSWDDIPNYLPDDSK---VFLA- 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051 223 vgcpgpeisqSSKVPVTSCLEFIWDR-PTLLVLGNEGSGLSQgvfATCQLL-------LTIlPgrhLPPGLESLNVSVAT 294
Cdd:cd18106   79 ----------DNNLPVQPYDSIDYCSgPIVLVIGGETHGVSP---EAYKLAhstggsrVHI-P---LANGVDSLNSAVAA 141


                 ....
gi 157819051 295 GILL 298
Cdd:cd18106  142 SILL 145
SpoU-like_TrmJ cd18093
SAM-dependent tRNA methylase related to TrmJ; tRNA methyltransferase TrmJ catalyzes the methyl ...
 144-301 7.66e-06

SAM-dependent tRNA methylase related to TrmJ; tRNA methyltransferase TrmJ catalyzes the methyl transfer from S-adenosyl-L-methionine (AdoMet) to the 2'-OH at position 32 in both tRNASer1 and tRNAGln2. It is part of the SpoU family of MTases, a subfamily of the SPOUT methyltransferase superfamily. The SPOUT methyltransferase superfamily is a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349966  Cd Length: 153  Bit Score: 45.18  E-value: 7.66e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051 144 LWLVLEGIQDPRNLGAVMRSAYFLGVD--RVITSRRNscPLTPVVSKASAGAMEVMD---VFasPDLASFLqAKAQqgwL 218
Cdd:cd18093    1 IRVVLVEPQHPGNIGAVARAMKNFGLSdlRLVNPRCD--DPEEAARALASGAEDVLEnakVY--DTLEEAL-ADCD---L 72

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051 219 VVGTVGcpgpEISQSSKVPVTS------CLEFIWDRPTLLVLGNEGSGLSQGVFATCQLLLTIlpgrhlP--PGLESLNV 290
Cdd:cd18093   73 VVATTA----RPRGLRRPLLTPrelaelLKEHAAGGKVALVFGREDSGLTNEELALCDLLVTI------PtnPEYPSLNL 142

                        170
                 ....*....|.
gi 157819051 291 SVATGILLHSI 301
Cdd:cd18093  143 AQAVAIVLYEL 153
SpoU-like_NHR cd18108
Nosiheptide-resistance methyltransferase (NHR); Nosiheptide-resistance methyltransferase (NHR) ...
 146-299 3.02e-03

Nosiheptide-resistance methyltransferase (NHR); Nosiheptide-resistance methyltransferase (NHR) confers resistance to the thiazole antibiotic nosiheptide via catalyzing 2'O-methylation of 23S rRNA at the nucleotide A1067. NHR is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349981  Cd Length: 144  Bit Score: 37.46  E-value: 3.02e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051 146 LVLEGIQDPRNLGAVMRSAYFLGVDRVITSRRNscpLTPV----VSKASAGAMEVMDVFASP--DLASFLQAKAQQgwLV 219
Cdd:cd18108    3 VVLDGVRIVGNIGAIVRTSLALGAAGIVLVDSD---LATVadrrLLRASRGYVFSLPVVLADreEAVAFLRDNDIP--LM 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157819051 220 VGTVGCPGPeISQSSKVPvtsclefiwdRPTLLVLGNEGSGLSQGVFATCQLLLTIlpgrHLPPGLESLNVSVATGILLH 299
Cdd:cd18108   78 TLDADGDVP-VKELGDRP----------DRLALVFGSEKGGPSGLFEEAAAGTVSI----PMSSSTESLNVSVSAGIALH 142
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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