GRAM domain; The GRAM domain is found in in glucosyltransferases, myotubularins and other putative membrane-associated proteins. Note the alignment is lacking the last two beta strands and alpha helix.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564364049   75 HKLFKdIPLEEMVVKVCSCALQRD-LLLHGRLYISPNWLCFHASLFGKDIKVVIPVASVQLIKKHKMAR-LLPNGLAITT 152
Cdd:pfam02893   4 RKKFK-LPPEERLIASYSCYLNRDgGPVQGRLYLTNYRLCFRSLPKGWSTKVVIPLVDIEEIEKLKGGAnLFPNGIQVET 82

                          90       100
                  ....*....|....*....|....
gi 564364049  153 NTSQKYVFVSLLSRDSVYDMLRRV 176
Cdd:pfam02893  83 GSNDKFSFAGFVTRDEAIEFILAL 106

Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain; Myotubularin-related proteins are a subfamily of protein tyrosine phosphatases (PTPs) that dephosphorylate D3-phosphorylated inositol lipids. Mutations in this family cause the human neuromuscular disorders myotubular myopathy and type 4B Charcot-Marie-Tooth syndrome. 6 of the 13 MTMRs (MTMRs 5, 9-13) contain naturally occurring substitutions of residues required for catalysis by PTP family enzymes. Although these proteins are predicted to be enzymatically inactive, they are thought to function as antagonists of endogenous phosphatase activity or interaction modules. Most MTMRs contain a N-terminal PH-GRAM domain, a Rac-induced recruitment domain (RID) domain, a PTP domain (which may be active or inactive), a SET-interaction domain, and a C-terminal coiled-coil region. In addition some members contain DENN domain N-terminal to the PH-GRAM domain and FYVE, PDZ, and PH domains C-terminal to the coiled-coil region. The GRAM domain, found in myotubularins, glucosyltransferases, and other putative membrane-associated proteins, is part of a larger motif with a pleckstrin homology (PH) domain fold.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564364049  84 EEMVVKVCSCAL-QRDLLLHGRLYISPNWLCFHASLFGKDIKVVIPVASVQLIKKHKMARLLPNGLAITTNTsQKYVFVS 162
Cdd:cd10570    1 IEKLGVRFCCALrPRKLPLEGTLYLSTYRLIFSSKADGDETKLVIPLVDITDVEKIAGASFLPSGLIITCKD-FRTIKFS 79

                         90
                 ....*....|....*
gi 564364049 163 LLSRDSVYDMLRRVC 177
Cdd:cd10570   80 FDSEDEAVKVIARVL 94

domain in glucosyltransferases, myotubularins and other putative membrane-associated proteins;

                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*...
gi 564364049    80 DIPLEEMVVKVCSCALQRDLLLHGRLYISPNWLCFHASLFGKDIKVVIPVASVQLIKK 137
Cdd:smart00568   1 KLPEEEKLIADYSCYLSRTGPVQGRLYISNYRLCFRSNLPGKLTKVVIPLADITRIEK 58

Autophagy-related protein 26/Sterol 3-beta-glucosyltransferase Pleckstrin homology (PH) domain, repeat 2; ATG26 (also called UGT51/UDP-glycosyltransferase 51), a member of the glycosyltransferase 28 family, resulting in the biosynthesis of sterol glucoside. ATG26 in decane metabolism and autophagy. There are 32 known autophagy-related (ATG) proteins, 17 are components of the core autophagic machinery essential for all autophagy-related pathways and 15 are the additional components required only for certain pathways or species. The core autophagic machinery includes 1) the ATG9 cycling system (ATG1, ATG2, ATG9, ATG13, ATG18, and ATG27), 2) the phosphatidylinositol 3-kinase complex (ATG6/VPS30, ATG14, VPS15, and ATG34), and 3) the ubiquitin-like protein system (ATG3, ATG4, ATG5, ATG7, ATG8, ATG10, ATG12, and ATG16). Less is known about how the core machinery is adapted or modulated with additional components to accommodate the nonselective sequestration of bulk cytosol (autophagosome formation) or selective sequestration of specific cargos (Cvt vesicle, pexophagosome, or bacteria-containing autophagosome formation). The pexophagosome-specific additions include the ATG30-ATG11-ATG17 receptor-adaptors complex, the coiled-coil protein ATG25, and the sterol glucosyltransferase ATG26. ATG26 is necessary for the degradation of medium peroxisomes. It contains 2 GRAM domains and a single PH domain. PH domains are only found in eukaryotes. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. PH domains also have diverse functions. They are often involved in targeting proteins to the plasma membrane, but few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564364049  92 SCALQRDLLLHGRLYISPNWLCFHASLFGKDIKVVIPVASVQLIKKHKMARLLPNGLAITTNTSQK--YVFVSLLSRDSV 169
Cdd:cd13216    9 YCYLIRVLPVYGKLYVSNNYLCFRSLLPGVSTKMILPLRDIENVEKEKGFRFGYSGLVIVIKGHEElfFEFSSKSSRDDC 88

                 ....
gi 564364049 170 YDML 173
Cdd:cd13216   89 ERLL 92

GRAM domain-containing protein 4 (GRAMDC4) Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain; GRAMDC4 is a membrane protein. Nothing is known about its function. Paralogs include: GRAMDC1A, GRAMDC1B, GRAMDC1C, GRAMDC2, GRAMDC3, and GRAMDC-like proteins. It contains a single PH-GRAM domain at its N-terminus. The GRAM domain is found in glucosyltransferases, myotubularins and other putative membrane-associated proteins. The GRAM domain is part of a larger motif with a pleckstrin homology (PH) domain fold.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564364049  93 CALQRDLLL-----HGRLYISPNWLCFHASLFGKDIKVVIPVASVQLIKKHKMARLLP-NGLAI--TTNTSQK-YVFVSL 163
Cdd:cd13221   11 CLINRDKMPtdyirNGRLYLTENYLCFESSSSSSKKNVVIPLTDITRIEKAKPYSFLPgSGMSIevSVSSADKpLFFGAM 90

                         90
                 ....*....|
gi 564364049 164 LSRDSVYDML 173
Cdd:cd13221   91 FRRDEFFDII 100

TCB1D8 and TCB1D9 family Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain, repeat 2; TBC1D8, TBC1D8B, TBC1D9 and TBC1D9B may act as a GTPase-activating proteins for Rab family protein(s). They all contain an N-terminal PH-GRAM domain and a C-terminal Rab-GTPase-TBC (Tre-2, BUB2p, and Cdc16p) domain. This cd contains the second repeat of the PH-GRAM domain. The GRAM domain is found in glucosyltransferases, myotubularins and other putative membrane-associated proteins. The GRAM domain is part of a larger motif with a pleckstrin homology (PH) domain fold.

                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 564364049 102 HGRLYISPNWLCFhASLFGKDIKVVIPVASVQLIKKHKMARLLPNGLAITTNTSQKYVFVSLLSRDSVYDMLRRVC 177
Cdd:cd13218   22 HGKMFISENYICF-ASKEGNLCSVIIPLREVLAIEKTNDSSVLPKPVIISIKGKMAFRFSELKDRDELVAKLRLKL 96

TBC1 domain family member 8 (TBC1D8; also called Vascular Rab-GAP/TBC-containing protein) Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain, repeat 2; TBC1D8 may act as a GTPase-activating protein for Rab family protein(s). TBC1D8 contains two N-terminal PH-GRAM domain and a C-terminal Rab-GTPase-TBC (Tre-2, BUB2p, and Cdc16p) domain. This cd contains the second repeat of the PH-GRAM domain. The GRAM domain is found in glucosyltransferases, myotubularins and other putative membrane-associated proteins. The GRAM domain is part of a larger motif with a pleckstrin homology (PH) domain fold.

                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 564364049 103 GRLYISPNWLCFhASLFGKDIKVVIPVASVQLIKKHKMARLLPNGLAITTNTSQKYVFVSLLSRDSVYDML 173
Cdd:cd13353   23 GRMYTSDSYICF-ASKEDGSCNVILPLREVVSIEKMEDTSLLPNPIIVSIRSKMAFQFIELKDRDSLVESL 92

TBC1 domain family member 8B (TBC1D8B) Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain, repeat 1; TBC1D8B may act as a GTPase-activating protein for Rab family protein(s). TBC1D8B contains an N-terminal PH-GRAM domain and a C-terminal Rab-GTPase-TBC (Tre-2, BUB2p, and Cdc16p) domain. This cd contains the first repeat of the PH-GRAM domain. The GRAM domain is found in glucosyltransferases, myotubularins and other putative membrane-associated proteins. The GRAM domain is part of a larger motif with a pleckstrin homology (PH) domain fold.

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 564364049  84 EEMVVKVCSCALQRDLL-LHGRLYISPNWLCFHASLFGKDIKVVIPVASVQLIKK 137
Cdd:cd13350    1 QEKLVTYYSCSYWRGRVpCQGWLYLSTNFLSFYSFLLGSEIKLIISWDEISKLEK 55