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Conserved domains on  [gi|1958658979|ref|XP_038942055|]
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EF-hand calcium-binding domain-containing protein 3 isoform X5 [Rattus norvegicus]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
EF-hand_8 pfam13833
EF-hand domain pair;
6521-6571 1.37e-06

EF-hand domain pair;


:

Pssm-ID: 404678 [Multi-domain]  Cd Length: 54  Bit Score: 48.47  E-value: 1.37e-06
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|..
gi 1958658979 6521 TGCIDSHGLMSTVAKLGMN-LNAYDIYNELKCADRDRDGKINFSDFIDVLTD 6571
Cdd:pfam13833    2 KGVITREELKRALALLGLKdLSEDEVDILFREFDTDGDGYISFDEFCVLLER 53
PTZ00184 super family cl33172
calmodulin; Provisional
 155-286 3.72e-06

calmodulin; Provisional


The actual alignment was detected with superfamily member PTZ00184:

Pssm-ID: 185504 [Multi-domain]  Cd Length: 149  Bit Score: 50.15  E-value: 3.72e-06
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958658979  155 EQMPEEFL-KYQELNALsrgcliFSKVRNGKIYVNDLPVVLHTLKISTNDSEMHQALKTVNIDVNGMLDFTEFLKVMNDI 233
Cdd:PTZ00184     3 DQLTEEQIaEFKEAFSL------FDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTLMARK 76

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|...
gi 1958658979  234 SPSTSQDPEFQNALKIFSKVKSGRVATGDVGAVLQSMDILVNPETLKDVIQHA 286
Cdd:PTZ00184    77 MKDTDSEEEIKEAFKVFDRDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREA 129
EFh_PEF super family cl25352
The penta-EF hand (PEF) family; The penta-EF hand (PEF) family contains a group of five ...
 4621-4743 4.93e-03

The penta-EF hand (PEF) family; The penta-EF hand (PEF) family contains a group of five EF-hand calcium-binding proteins, including several classical calpain large catalytic subunits (CAPN1, 2, 3, 8, 9, 11, 12, 13, 14), two calpain small subunits (CAPNS1 and CAPNS2), as well as non-calpain PEF proteins, ALG-2 (apoptosis-linked gene 2, also termed programmed cell death protein 6, PDCD6), peflin, sorcin, and grancalcin. Based on the sequence similarity of EF1 hand, ALG-2 and peflin have been classified into group I PEF proteins. Calcium-dependent protease calpain subfamily members, sorcin and grancalcin, are group II PEF proteins. Calpains (EC 3.4.22.17) are calcium-activated intracellular cysteine proteases that play important roles in the degradation or functional modulation in a variety of substrates. They have been implicated in a number of physiological processes such as cell cycle progression, remodeling of cytoskeletal-cell membrane attachments, signal transduction, gene expression and apoptosis. ALG-2 is a pro-apoptotic factor that forms a homodimer in the cell or a heterodimer with its closest paralog peflin through their EF5s. Peflin is a 30-kD PEF protein with a longer N-terminal hydrophobic domain than any other member of the PEF family, and it contains nine nonapeptide (A/PPGGPYGGP) repeats. It exists only as a heterodimer with ALG-2. The dissociation of heterodimer occurs in the presence of Ca2+. ALG-2 interacts with various proteins in a Ca2+-dependent manner. Sorcin (for soluble resistance-related calcium binding protein) is a soluble resistance-related calcium-binding protein that participates in the regulation of calcium homeostasis in cells. Grancalcin is a cytosolic Ca2+-binding protein specifically expressed in neutrophils and monocytes/macrophages. It plays a key role in leukocyte-specific functions that are responsible for host defense. Grancalcin can form a heterodimer together with sorcin. Members in this family contain five EF-hand motifs attached to an N-terminal region of variable length containing one or more short Gly/Pro-rich sequences. These proteins form homodimers or heterodimers through pairing between the 5th EF-hands from the two molecules. Unlike calmodulin, the PEF domains do not undergo major conformational changes upon binding Ca2+.


The actual alignment was detected with superfamily member cd15897:

Pssm-ID: 355382 [Multi-domain]  Cd Length: 165  Bit Score: 41.65  E-value: 4.93e-03
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958658979 4621 EGKIDVSNLNNILKSLGIKLREEEFAKVSKDLPV-----DSSGKAEMKKVMDIIKTTTDDDKIFKNRliesvTSLQGGKV 4695
Cdd:cd15897     13 DGEISATELQQALSNVGWTHFDLGFSLETCRSMIammdrDHSGKLNFSEFKGLWNYIKAWQEIFRTY-----DTDGSGTI 87

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|...
gi 1958658979 4696 DSNNLDTVLDNMGIKLSNTELQDL-----KQSMNVGVDNKISLQTLMQKMKDF 4743
Cdd:cd15897     88 DSNELRQALSGAGYRLSEQTYDIIirrydRGRGNIDFDDFIQCCVRLQRLTDA 140
FRQ1 super family cl34916
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
2265-2360 5.34e-03

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


The actual alignment was detected with superfamily member COG5126:

Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 40.93  E-value: 5.34e-03
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958658979 2265 LSKDDLHSA-KAILTEILSHVQEDEDGKITIEEFVNEFANLLKALKSKRVKEMLNtKFDLNN---INAAsEIKQNLNALG 2340
Cdd:COG5126     22 LERDDFEALfRRLWATLFSEADTDGDGRISREEFVAGMESLFEATVEPFARAAFD-LLDTDGdgkISAD-EFRRLLTALG 99

                           90       100
                   ....*....|....*....|
gi 1958658979 2341 IhpTESDIQRAMENLASSGD 2360
Cdd:COG5126    100 V--SEEEADELFARLDTDGD 117
 
Name Accession Description Interval E-value
EF-hand_8 pfam13833
EF-hand domain pair;
6521-6571 1.37e-06

EF-hand domain pair;


Pssm-ID: 404678 [Multi-domain]  Cd Length: 54  Bit Score: 48.47  E-value: 1.37e-06
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|..
gi 1958658979 6521 TGCIDSHGLMSTVAKLGMN-LNAYDIYNELKCADRDRDGKINFSDFIDVLTD 6571
Cdd:pfam13833    2 KGVITREELKRALALLGLKdLSEDEVDILFREFDTDGDGYISFDEFCVLLER 53
PTZ00184 PTZ00184
calmodulin; Provisional
 155-286 3.72e-06

calmodulin; Provisional


Pssm-ID: 185504 [Multi-domain]  Cd Length: 149  Bit Score: 50.15  E-value: 3.72e-06
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958658979  155 EQMPEEFL-KYQELNALsrgcliFSKVRNGKIYVNDLPVVLHTLKISTNDSEMHQALKTVNIDVNGMLDFTEFLKVMNDI 233
Cdd:PTZ00184     3 DQLTEEQIaEFKEAFSL------FDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTLMARK 76

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|...
gi 1958658979  234 SPSTSQDPEFQNALKIFSKVKSGRVATGDVGAVLQSMDILVNPETLKDVIQHA 286
Cdd:PTZ00184    77 MKDTDSEEEIKEAFKVFDRDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREA 129
PTZ00183 PTZ00183
centrin; Provisional
 6497-6572 2.48e-05

centrin; Provisional


Pssm-ID: 185503 [Multi-domain]  Cd Length: 158  Bit Score: 48.15  E-value: 2.48e-05
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1958658979 6497 KETKLSPAQLEAFRNAYNFFTKDRTGCIDSHGLMSTVAKLGMNLNAYDIYNELKCADRDRDGKINFSDFIDVLTDK 6572
Cdd:PTZ00183     7 ERPGLTEDQKKEIREAFDLFDTDGSGTIDPKELKVAMRSLGFEPKKEEIKQMIADVDKDGSGKIDFEEFLDIMTKK 82
EFh_PEF cd15897
The penta-EF hand (PEF) family; The penta-EF hand (PEF) family contains a group of five ...
 4621-4743 4.93e-03

The penta-EF hand (PEF) family; The penta-EF hand (PEF) family contains a group of five EF-hand calcium-binding proteins, including several classical calpain large catalytic subunits (CAPN1, 2, 3, 8, 9, 11, 12, 13, 14), two calpain small subunits (CAPNS1 and CAPNS2), as well as non-calpain PEF proteins, ALG-2 (apoptosis-linked gene 2, also termed programmed cell death protein 6, PDCD6), peflin, sorcin, and grancalcin. Based on the sequence similarity of EF1 hand, ALG-2 and peflin have been classified into group I PEF proteins. Calcium-dependent protease calpain subfamily members, sorcin and grancalcin, are group II PEF proteins. Calpains (EC 3.4.22.17) are calcium-activated intracellular cysteine proteases that play important roles in the degradation or functional modulation in a variety of substrates. They have been implicated in a number of physiological processes such as cell cycle progression, remodeling of cytoskeletal-cell membrane attachments, signal transduction, gene expression and apoptosis. ALG-2 is a pro-apoptotic factor that forms a homodimer in the cell or a heterodimer with its closest paralog peflin through their EF5s. Peflin is a 30-kD PEF protein with a longer N-terminal hydrophobic domain than any other member of the PEF family, and it contains nine nonapeptide (A/PPGGPYGGP) repeats. It exists only as a heterodimer with ALG-2. The dissociation of heterodimer occurs in the presence of Ca2+. ALG-2 interacts with various proteins in a Ca2+-dependent manner. Sorcin (for soluble resistance-related calcium binding protein) is a soluble resistance-related calcium-binding protein that participates in the regulation of calcium homeostasis in cells. Grancalcin is a cytosolic Ca2+-binding protein specifically expressed in neutrophils and monocytes/macrophages. It plays a key role in leukocyte-specific functions that are responsible for host defense. Grancalcin can form a heterodimer together with sorcin. Members in this family contain five EF-hand motifs attached to an N-terminal region of variable length containing one or more short Gly/Pro-rich sequences. These proteins form homodimers or heterodimers through pairing between the 5th EF-hands from the two molecules. Unlike calmodulin, the PEF domains do not undergo major conformational changes upon binding Ca2+.


Pssm-ID: 320054 [Multi-domain]  Cd Length: 165  Bit Score: 41.65  E-value: 4.93e-03
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958658979 4621 EGKIDVSNLNNILKSLGIKLREEEFAKVSKDLPV-----DSSGKAEMKKVMDIIKTTTDDDKIFKNRliesvTSLQGGKV 4695
Cdd:cd15897     13 DGEISATELQQALSNVGWTHFDLGFSLETCRSMIammdrDHSGKLNFSEFKGLWNYIKAWQEIFRTY-----DTDGSGTI 87

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|...
gi 1958658979 4696 DSNNLDTVLDNMGIKLSNTELQDL-----KQSMNVGVDNKISLQTLMQKMKDF 4743
Cdd:cd15897     88 DSNELRQALSGAGYRLSEQTYDIIirrydRGRGNIDFDDFIQCCVRLQRLTDA 140
FRQ1 COG5126
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
2265-2360 5.34e-03

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 40.93  E-value: 5.34e-03
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958658979 2265 LSKDDLHSA-KAILTEILSHVQEDEDGKITIEEFVNEFANLLKALKSKRVKEMLNtKFDLNN---INAAsEIKQNLNALG 2340
Cdd:COG5126     22 LERDDFEALfRRLWATLFSEADTDGDGRISREEFVAGMESLFEATVEPFARAAFD-LLDTDGdgkISAD-EFRRLLTALG 99

                           90       100
                   ....*....|....*....|
gi 1958658979 2341 IhpTESDIQRAMENLASSGD 2360
Cdd:COG5126    100 V--SEEEADELFARLDTDGD 117
 
Name Accession Description Interval E-value
EF-hand_8 pfam13833
EF-hand domain pair;
6521-6571 1.37e-06

EF-hand domain pair;


Pssm-ID: 404678 [Multi-domain]  Cd Length: 54  Bit Score: 48.47  E-value: 1.37e-06
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|..
gi 1958658979 6521 TGCIDSHGLMSTVAKLGMN-LNAYDIYNELKCADRDRDGKINFSDFIDVLTD 6571
Cdd:pfam13833    2 KGVITREELKRALALLGLKdLSEDEVDILFREFDTDGDGYISFDEFCVLLER 53
PTZ00184 PTZ00184
calmodulin; Provisional
 155-286 3.72e-06

calmodulin; Provisional


Pssm-ID: 185504 [Multi-domain]  Cd Length: 149  Bit Score: 50.15  E-value: 3.72e-06
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958658979  155 EQMPEEFL-KYQELNALsrgcliFSKVRNGKIYVNDLPVVLHTLKISTNDSEMHQALKTVNIDVNGMLDFTEFLKVMNDI 233
Cdd:PTZ00184     3 DQLTEEQIaEFKEAFSL------FDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTLMARK 76

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|...
gi 1958658979  234 SPSTSQDPEFQNALKIFSKVKSGRVATGDVGAVLQSMDILVNPETLKDVIQHA 286
Cdd:PTZ00184    77 MKDTDSEEEIKEAFKVFDRDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREA 129
PTZ00183 PTZ00183
centrin; Provisional
 6497-6572 2.48e-05

centrin; Provisional


Pssm-ID: 185503 [Multi-domain]  Cd Length: 158  Bit Score: 48.15  E-value: 2.48e-05
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1958658979 6497 KETKLSPAQLEAFRNAYNFFTKDRTGCIDSHGLMSTVAKLGMNLNAYDIYNELKCADRDRDGKINFSDFIDVLTDK 6572
Cdd:PTZ00183     7 ERPGLTEDQKKEIREAFDLFDTDGSGTIDPKELKVAMRSLGFEPKKEEIKQMIADVDKDGSGKIDFEEFLDIMTKK 82
PTZ00183 PTZ00183
centrin; Provisional
 6512-6575 3.66e-03

centrin; Provisional


Pssm-ID: 185503 [Multi-domain]  Cd Length: 158  Bit Score: 41.60  E-value: 3.66e-03
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1958658979 6512 AYNFFTKDRTGCIDSHGLMSTVAKLGMNLNAYDIYNELKCADRDRDGKINFSDFIDVLTDKKLF 6575
Cdd:PTZ00183    95 AFRLFDDDKTGKISLKNLKRVAKELGETITDEELQEMIDEADRNGDGEISEEEFYRIMKKTNLF 158
EFh_PEF cd15897
The penta-EF hand (PEF) family; The penta-EF hand (PEF) family contains a group of five ...
 4621-4743 4.93e-03

The penta-EF hand (PEF) family; The penta-EF hand (PEF) family contains a group of five EF-hand calcium-binding proteins, including several classical calpain large catalytic subunits (CAPN1, 2, 3, 8, 9, 11, 12, 13, 14), two calpain small subunits (CAPNS1 and CAPNS2), as well as non-calpain PEF proteins, ALG-2 (apoptosis-linked gene 2, also termed programmed cell death protein 6, PDCD6), peflin, sorcin, and grancalcin. Based on the sequence similarity of EF1 hand, ALG-2 and peflin have been classified into group I PEF proteins. Calcium-dependent protease calpain subfamily members, sorcin and grancalcin, are group II PEF proteins. Calpains (EC 3.4.22.17) are calcium-activated intracellular cysteine proteases that play important roles in the degradation or functional modulation in a variety of substrates. They have been implicated in a number of physiological processes such as cell cycle progression, remodeling of cytoskeletal-cell membrane attachments, signal transduction, gene expression and apoptosis. ALG-2 is a pro-apoptotic factor that forms a homodimer in the cell or a heterodimer with its closest paralog peflin through their EF5s. Peflin is a 30-kD PEF protein with a longer N-terminal hydrophobic domain than any other member of the PEF family, and it contains nine nonapeptide (A/PPGGPYGGP) repeats. It exists only as a heterodimer with ALG-2. The dissociation of heterodimer occurs in the presence of Ca2+. ALG-2 interacts with various proteins in a Ca2+-dependent manner. Sorcin (for soluble resistance-related calcium binding protein) is a soluble resistance-related calcium-binding protein that participates in the regulation of calcium homeostasis in cells. Grancalcin is a cytosolic Ca2+-binding protein specifically expressed in neutrophils and monocytes/macrophages. It plays a key role in leukocyte-specific functions that are responsible for host defense. Grancalcin can form a heterodimer together with sorcin. Members in this family contain five EF-hand motifs attached to an N-terminal region of variable length containing one or more short Gly/Pro-rich sequences. These proteins form homodimers or heterodimers through pairing between the 5th EF-hands from the two molecules. Unlike calmodulin, the PEF domains do not undergo major conformational changes upon binding Ca2+.


Pssm-ID: 320054 [Multi-domain]  Cd Length: 165  Bit Score: 41.65  E-value: 4.93e-03
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958658979 4621 EGKIDVSNLNNILKSLGIKLREEEFAKVSKDLPV-----DSSGKAEMKKVMDIIKTTTDDDKIFKNRliesvTSLQGGKV 4695
Cdd:cd15897     13 DGEISATELQQALSNVGWTHFDLGFSLETCRSMIammdrDHSGKLNFSEFKGLWNYIKAWQEIFRTY-----DTDGSGTI 87

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|...
gi 1958658979 4696 DSNNLDTVLDNMGIKLSNTELQDL-----KQSMNVGVDNKISLQTLMQKMKDF 4743
Cdd:cd15897     88 DSNELRQALSGAGYRLSEQTYDIIirrydRGRGNIDFDDFIQCCVRLQRLTDA 140
FRQ1 COG5126
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
2265-2360 5.34e-03

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 40.93  E-value: 5.34e-03
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958658979 2265 LSKDDLHSA-KAILTEILSHVQEDEDGKITIEEFVNEFANLLKALKSKRVKEMLNtKFDLNN---INAAsEIKQNLNALG 2340
Cdd:COG5126     22 LERDDFEALfRRLWATLFSEADTDGDGRISREEFVAGMESLFEATVEPFARAAFD-LLDTDGdgkISAD-EFRRLLTALG 99

                           90       100
                   ....*....|....*....|
gi 1958658979 2341 IhpTESDIQRAMENLASSGD 2360
Cdd:COG5126    100 V--SEEEADELFARLDTDGD 117
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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