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Conserved domains on  [gi|1958680285|ref|XP_038949323|]
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Krueppel-like factor 12 isoform X8 [Rattus norvegicus]

Protein Classification

C2H2-type zinc finger protein( domain architecture ID 15750743)

Cys2His2 (C2H2)-type zinc finger protein may be involved in transcriptional regulation

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
KLF8_12_N super family cl40450
N-terminal domain of Kruppel-like factor (KLF) 8, KLF12, and similar proteins; Kruppel-like ...
 1-174 3.82e-75

N-terminal domain of Kruppel-like factor (KLF) 8, KLF12, and similar proteins; Kruppel-like transcription factors (also known as Krueppel-like transcription factors, KLFs) belong to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Although these factors bind to similar elements in vitro, they have distinct activities in vivo depending on their expression profile and the sequence of the N-terminal activation/repression domain, which differ between members. This model represents the related N-terminal activation/repression domains of KLF8 and KLF12.


The actual alignment was detected with superfamily member cd21441:

Pssm-ID: 424081 [Multi-domain]  Cd Length: 197  Bit Score: 230.28  E-value: 3.82e-75
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958680285   1 MEAIPLLLNNVKGEPPEDSLPVDHFQTQTEPVDLSINKARTSPTAASSSPVSMTASASSPSSTSTSSSSSSRPASSpTVI 80
Cdd:cd21441    25 MEAVPLLLNNVKAEPPEDSLSTDHFQTQTEPVDLSINKARTSPTAVSSSPVSMTASASPSSSSSSSSSSSRPASSP-TVI 103

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958680285  81 TSVSSASSSSTVLSPGPLVASASGMGGQQFLHIIHPVPPSSPMNLQSNKLSHVHRIPVVVQSVPVVYTAVRSPGNVNNTI 160
Cdd:cd21441   104 TSVSSASSVPTVLTPGPLVASASGVGGQQFLHIIHPVPPSSPMNLQSNKLSHVHRIPVVVQSVPVVYTAVRSPGNVNNTI 183

                         170
                  ....*....|....
gi 1958680285 161 VVPLLEDGRSHGKA 174
Cdd:cd21441   184 VVPLLEDGRSHGKA 197
zf-H2C2_2 pfam13465
Zinc-finger double domain;
313-338 1.42e-05

Zinc-finger double domain;


:

Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 41.20  E-value: 1.42e-05
                          10        20
                  ....*....|....*....|....*.
gi 1958680285 313 ELTRHYRKHTGVKPFKCADCDRSFSR 338
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPECGKSFKS 26
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
175-351 1.97e-04

FOG: Zn-finger [General function prediction only];


:

Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 43.15  E-value: 1.97e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958680285 175 QMEPRGLSPRQSKSDSDDDDLPNVTLDSVNETGSTALSIARAVQEVHPSPVSRVRGNRMNNQkfaCSISPFSIESTRRQR 254
Cdd:COG5048   233 SQLSPKSLLSQSPSSLSSSDSSSSASESPRSSLPTASSQSSSPNESDSSSEKGFSLPIKSKQ---CNISFSRSSPLTRHL 309

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958680285 255 RSESPDSRKRRIHRCDFEGCNKVYTKSSHLKAHRRTHTGEKPYKCTWEGCTWKF-----ARSDELTRHYRKHTGVKPFKC 329
Cdd:COG5048   310 RSVNHSGESLKPFSCPYSLCGKLFSRNDALKRHILLHTSISPAKEKLLNSSSKFspllnNEPPQSLQQYKDLKNDKKSET 389

                         170       180
                  ....*....|....*....|....
gi 1958680285 330 AD--CDRSFSRSDHLALHRRRHML 351
Cdd:COG5048   390 LSnsCIRNFKRDSNLSLHIITHLS 413
 
Name Accession Description Interval E-value
KLF12_N cd21441
N-terminal domain of Kruppel-like factor 12; Kruppel-like factor 12 (also known as ...
 1-174 3.82e-75

N-terminal domain of Kruppel-like factor 12; Kruppel-like factor 12 (also known as Krueppel-like transcription factor 12, KLF12) regulates, by transcriptionally repressing Nur77 expression, endometrial decidualization, which is a prerequisite for successful implantation and the establishment of pregnancy. It is involved in the maturation processes of kidney collecting ducts after birth, and is able to increase the promoter activity of the UT-A1 urea transporter promoter by binding to the CACCC motif. KLF12 has also been found to promote colorectal cancer growth is also involved in the invasion and apoptosis of basal-like breast carcinoma. KLF12 belongs to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Although these factors bind to similar elements in vitro, they have distinct activities in vivo depending on their expression profile and the sequence of the N-terminal activation/repression domain, which differ between members. KLF12 contains an N-terminal domain that is related to the N-terminal repression domain of KLF8.


Pssm-ID: 410608 [Multi-domain]  Cd Length: 197  Bit Score: 230.28  E-value: 3.82e-75
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958680285   1 MEAIPLLLNNVKGEPPEDSLPVDHFQTQTEPVDLSINKARTSPTAASSSPVSMTASASSPSSTSTSSSSSSRPASSpTVI 80
Cdd:cd21441    25 MEAVPLLLNNVKAEPPEDSLSTDHFQTQTEPVDLSINKARTSPTAVSSSPVSMTASASPSSSSSSSSSSSRPASSP-TVI 103

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958680285  81 TSVSSASSSSTVLSPGPLVASASGMGGQQFLHIIHPVPPSSPMNLQSNKLSHVHRIPVVVQSVPVVYTAVRSPGNVNNTI 160
Cdd:cd21441   104 TSVSSASSVPTVLTPGPLVASASGVGGQQFLHIIHPVPPSSPMNLQSNKLSHVHRIPVVVQSVPVVYTAVRSPGNVNNTI 183

                         170
                  ....*....|....
gi 1958680285 161 VVPLLEDGRSHGKA 174
Cdd:cd21441   184 VVPLLEDGRSHGKA 197
zf-H2C2_2 pfam13465
Zinc-finger double domain;
313-338 1.42e-05

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 41.20  E-value: 1.42e-05
                          10        20
                  ....*....|....*....|....*.
gi 1958680285 313 ELTRHYRKHTGVKPFKCADCDRSFSR 338
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPECGKSFKS 26
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
175-351 1.97e-04

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 43.15  E-value: 1.97e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958680285 175 QMEPRGLSPRQSKSDSDDDDLPNVTLDSVNETGSTALSIARAVQEVHPSPVSRVRGNRMNNQkfaCSISPFSIESTRRQR 254
Cdd:COG5048   233 SQLSPKSLLSQSPSSLSSSDSSSSASESPRSSLPTASSQSSSPNESDSSSEKGFSLPIKSKQ---CNISFSRSSPLTRHL 309

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958680285 255 RSESPDSRKRRIHRCDFEGCNKVYTKSSHLKAHRRTHTGEKPYKCTWEGCTWKF-----ARSDELTRHYRKHTGVKPFKC 329
Cdd:COG5048   310 RSVNHSGESLKPFSCPYSLCGKLFSRNDALKRHILLHTSISPAKEKLLNSSSKFspllnNEPPQSLQQYKDLKNDKKSET 389

                         170       180
                  ....*....|....*....|....
gi 1958680285 330 AD--CDRSFSRSDHLALHRRRHML 351
Cdd:COG5048   390 LSnsCIRNFKRDSNLSLHIITHLS 413
zf-C2H2 pfam00096
Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two ...
 267-291 9.16e-03

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.


Pssm-ID: 395048 [Multi-domain]  Cd Length: 23  Bit Score: 33.43  E-value: 9.16e-03
                          10        20
                  ....*....|....*....|....*
gi 1958680285 267 HRCdfEGCNKVYTKSSHLKAHRRTH 291
Cdd:pfam00096   1 YKC--PDCGKSFSRKSNLKRHLRTH 23
 
Name Accession Description Interval E-value
KLF12_N cd21441
N-terminal domain of Kruppel-like factor 12; Kruppel-like factor 12 (also known as ...
 1-174 3.82e-75

N-terminal domain of Kruppel-like factor 12; Kruppel-like factor 12 (also known as Krueppel-like transcription factor 12, KLF12) regulates, by transcriptionally repressing Nur77 expression, endometrial decidualization, which is a prerequisite for successful implantation and the establishment of pregnancy. It is involved in the maturation processes of kidney collecting ducts after birth, and is able to increase the promoter activity of the UT-A1 urea transporter promoter by binding to the CACCC motif. KLF12 has also been found to promote colorectal cancer growth is also involved in the invasion and apoptosis of basal-like breast carcinoma. KLF12 belongs to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Although these factors bind to similar elements in vitro, they have distinct activities in vivo depending on their expression profile and the sequence of the N-terminal activation/repression domain, which differ between members. KLF12 contains an N-terminal domain that is related to the N-terminal repression domain of KLF8.


Pssm-ID: 410608 [Multi-domain]  Cd Length: 197  Bit Score: 230.28  E-value: 3.82e-75
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958680285   1 MEAIPLLLNNVKGEPPEDSLPVDHFQTQTEPVDLSINKARTSPTAASSSPVSMTASASSPSSTSTSSSSSSRPASSpTVI 80
Cdd:cd21441    25 MEAVPLLLNNVKAEPPEDSLSTDHFQTQTEPVDLSINKARTSPTAVSSSPVSMTASASPSSSSSSSSSSSRPASSP-TVI 103

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958680285  81 TSVSSASSSSTVLSPGPLVASASGMGGQQFLHIIHPVPPSSPMNLQSNKLSHVHRIPVVVQSVPVVYTAVRSPGNVNNTI 160
Cdd:cd21441   104 TSVSSASSVPTVLTPGPLVASASGVGGQQFLHIIHPVPPSSPMNLQSNKLSHVHRIPVVVQSVPVVYTAVRSPGNVNNTI 183

                         170
                  ....*....|....
gi 1958680285 161 VVPLLEDGRSHGKA 174
Cdd:cd21441   184 VVPLLEDGRSHGKA 197
KLF8_12_N cd21093
N-terminal domain of Kruppel-like factor (KLF) 8, KLF12, and similar proteins; Kruppel-like ...
 5-174 2.83e-55

N-terminal domain of Kruppel-like factor (KLF) 8, KLF12, and similar proteins; Kruppel-like transcription factors (also known as Krueppel-like transcription factors, KLFs) belong to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Although these factors bind to similar elements in vitro, they have distinct activities in vivo depending on their expression profile and the sequence of the N-terminal activation/repression domain, which differ between members. This model represents the related N-terminal activation/repression domains of KLF8 and KLF12.


Pssm-ID: 410606 [Multi-domain]  Cd Length: 172  Bit Score: 178.43  E-value: 2.83e-55
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958680285   5 PLLLNNVKGEPPEDSLPVDHFQTQTEPVDLSINKARTSPTAASSSPVsmtasaSSPSSTSTSSSSSSRPASSPTVITSVS 84
Cdd:cd21093    13 PLLLNNIKTEPPEELLSSDHSQPQTEPVDLSINKARTSPTAVSSSPV------SMSSSISSSSSSSPRPASSPTVITSVS 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958680285  85 SASSSSTVLSPGPLVASASGMGGQQFLHIIHPVPPSSpmnlQSNKLSHVHRIPVVVQSVPVVYTAVRSPGNVnNTIVVPL 164
Cdd:cd21093    87 SASAIPTVLSPGSILASAQGVGGQQILHVIHTVPSVS----LPNKMSHLHTIPVVVQSLPVVYTAVRSDGNT-ATITVPL 161

                         170
                  ....*....|.
gi 1958680285 165 LE-DGRSHGKA 174
Cdd:cd21093   162 IGgDGKSHGKV 172
KLF8_N cd21440
N-terminal domain of Kruppel-like factor 8; Kruppel-like factor 8 (also known as Krueppel-like ...
 5-172 3.95e-19

N-terminal domain of Kruppel-like factor 8; Kruppel-like factor 8 (also known as Krueppel-like transcription factor 8, KLF8) is a CACCC-box binding protein that associates with C-terminal Binding Protein (CtBP) and represses transcription. It plays an essential role in the regulation of the cell cycle, apoptosis, and differentiation. It has been identified as a key component of the transcription factor network that controls terminal differentiation during adipogenesis. It also plays an important role in the formation of several human tumors, including the promotion of tumorigenesis, invasion, and metastasis of colorectal cancer cells, and the progression of pancreatic cancer. KLF8 belongs to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Although these factors bind to similar elements in vitro, they have distinct activities in vivo depending on their expression profile and the sequence of the N-terminal activation/repression domain, which differ between members. KLF8 contains an N-terminal repression domain that is related to that of KLF12.


Pssm-ID: 410607 [Multi-domain]  Cd Length: 169  Bit Score: 83.35  E-value: 3.95e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958680285   5 PLLLNNVKGEPPEDSLPVDHFQTQTEPVDLSINKARTSPTAASSSPVSMTASASSPSSTSTSSSSSSRPASSPTVItsvs 84
Cdd:cd21440    13 PALLSDIKTEPPEELLASDCSQPQAEPVDLSLHKPKAPLQPPSVLSPSPMILSVSPSAPQSLVSSTGTGMGTTSAI---- 88

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958680285  85 sasssSTVLSPGPLVASASGMGGQQFLHIIHPVPpssPMNLQSnKLSHVHRIPVVVQSVPVVYTAVRSPGnVNNTIVVPL 164
Cdd:cd21440    89 -----PAVLSPGSILASSQGSGGQQILHVIHTIP---SVNLPS-KMGNLQTIPVVVQSLPVVYTTLPTDG-VTAAITVPL 158


                  ....*....
gi 1958680285 165 L-EDGRSHG 172
Cdd:cd21440   159 IgGDGKNAG 167
zf-H2C2_2 pfam13465
Zinc-finger double domain;
313-338 1.42e-05

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 41.20  E-value: 1.42e-05
                          10        20
                  ....*....|....*....|....*.
gi 1958680285 313 ELTRHYRKHTGVKPFKCADCDRSFSR 338
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPECGKSFKS 26
zf-C2H2 pfam00096
Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two ...
 327-349 1.49e-04

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.


Pssm-ID: 395048 [Multi-domain]  Cd Length: 23  Bit Score: 38.44  E-value: 1.49e-04
                          10        20
                  ....*....|....*....|...
gi 1958680285 327 FKCADCDRSFSRSDHLALHRRRH 349
Cdd:pfam00096   1 YKCPDCGKSFSRKSNLKRHLRTH 23
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
175-351 1.97e-04

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 43.15  E-value: 1.97e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958680285 175 QMEPRGLSPRQSKSDSDDDDLPNVTLDSVNETGSTALSIARAVQEVHPSPVSRVRGNRMNNQkfaCSISPFSIESTRRQR 254
Cdd:COG5048   233 SQLSPKSLLSQSPSSLSSSDSSSSASESPRSSLPTASSQSSSPNESDSSSEKGFSLPIKSKQ---CNISFSRSSPLTRHL 309

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958680285 255 RSESPDSRKRRIHRCDFEGCNKVYTKSSHLKAHRRTHTGEKPYKCTWEGCTWKF-----ARSDELTRHYRKHTGVKPFKC 329
Cdd:COG5048   310 RSVNHSGESLKPFSCPYSLCGKLFSRNDALKRHILLHTSISPAKEKLLNSSSKFspllnNEPPQSLQQYKDLKNDKKSET 389

                         170       180
                  ....*....|....*....|....
gi 1958680285 330 AD--CDRSFSRSDHLALHRRRHML 351
Cdd:COG5048   390 LSnsCIRNFKRDSNLSLHIITHLS 413
SFP1 COG5189
Putative transcriptional repressor regulating G2/M transition [Transcription / Cell division ...
 265-346 3.58e-03

Putative transcriptional repressor regulating G2/M transition [Transcription / Cell division and chromosome partitioning];


Pssm-ID: 227516 [Multi-domain]  Cd Length: 423  Bit Score: 38.93  E-value: 3.58e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958680285 265 RIHRCDFEGCNKVYTKSSHLKAHRRT-HTGEKPYKCTWEGCTWKFARSDeltrhyrkhtgvKPFKCADCDRSFSRSDHLA 343
Cdd:COG5189   348 KPYKCPVEGCNKKYKNQNGLKYHMLHgHQNQKLHENPSPEKMNIFSAKD------------KPYRCEVCDKRYKNLNGLK 415


                  ...
gi 1958680285 344 LHR 346
Cdd:COG5189   416 YHR 418
KLF3_N cd21577
N-terminal domain of Kruppel-like factor 3; Kruppel-like factor 3 (KLF3; also called ...
 16-50 3.68e-03

N-terminal domain of Kruppel-like factor 3; Kruppel-like factor 3 (KLF3; also called Krueppel-like factor 3 and originally called Basic Kruppel-like Factor/BKLF), was the third member of the KLF family of zinc finger transcription factors to be discovered. KLF3 possesses a wide range of biological impacts on regulating apoptosis, differentiation, and proliferation in various tissues during the entire progression process. It has been proposed as a tumor suppressor in colorectal cancer. It appears to function predominantly as a repressor of transcription, turning genes off by recruiting the C-terminal Binding Protein co-repressors CtBP1 and CtBP2. CtBP docks onto a short motif (residues 61-65) in the N-terminus of KLF3, through the Proline-X-Aspartate-Leucine-Serine (PXDLS) motif. CtBP in turn recruits histone modifying enzymes to alter chromatin and repress gene expression. KLF3 belongs to a family of proteins, called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specificity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF3.


Pssm-ID: 410554 [Multi-domain]  Cd Length: 214  Bit Score: 38.10  E-value: 3.68e-03
                          10        20        30
                  ....*....|....*....|....*....|....*
gi 1958680285  16 PEDSLPVDHfQTQTEPVDLSINKaRTSPTAASSSP 50
Cdd:cd21577     7 METSFYSPS-HSQLEPVDLSLSK-RSSPPSSSSSS 39
zf-C2H2 pfam00096
Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two ...
 267-291 9.16e-03

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.


Pssm-ID: 395048 [Multi-domain]  Cd Length: 23  Bit Score: 33.43  E-value: 9.16e-03
                          10        20
                  ....*....|....*....|....*
gi 1958680285 267 HRCdfEGCNKVYTKSSHLKAHRRTH 291
Cdd:pfam00096   1 YKC--PDCGKSFSRKSNLKRHLRTH 23
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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