National Center for
Biotechnology Information

3JCM: Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP

PDB ID:

MMDB ID:

136652

PDB Deposition Date:

2015/12/23

Updated in MMDB:

2022/11

Experimental Method:

electron microscopy

Resolution:

3.8 Å

Source Organism:

Saccharomyces cerevisiae S288C▼

Taxonomy	Molecule
Saccharomyces cerevisiae S288C	A, B, I, G, K, L, M, H, N, R, S, T, U, V, W, X, J, O, P, Q, Y, Z, a, b, c, d, e, f, g, h, D, E, F
synthetic construct	C

Similar Structures:

Biological Unit for 3JCM: 34-meric; determined by author

Molecular Components in 3JCM
Label	Count	Molecule
Proteins (30 molecules)
A	1	Pre-mrna-splicing Factor 8
B	1	U4/u6 Small Nuclear Ribonucleoprotein Prp4
I	1	Pre-mrna-processing Factor 31
G	1	Pre-mrna-splicing Factor 6
K	1	U4/u6 Small Nuclear Ribonucleoprotein Prp3
L	1	Spliceosomal Protein Dib1
M	1	13 KDA Ribonucleoprotein-associated Protein
H	1	Pre-mrna-splicing Factor Snu114
N	1	Pre-mrna-splicing Helicase Brr2
R J	2	Small Nuclear Ribonucleoprotein SM D3
S O	2	Small Nuclear Ribonucleoprotein-associated Protein B
T P	2	Small Nuclear Ribonucleoprotein SM D1
U Q	2	Small Nuclear Ribonucleoprotein SM D2
V Y	2	Small Nuclear Ribonucleoprotein E
W Z	2	Small Nuclear Ribonucleoprotein F
X a	2	Small Nuclear Ribonucleoprotein G
b	1	U6 Snrna-associated Sm-like Protein Lsm8
c	1	U6 Snrna-associated Sm-like Protein Lsm2
d	1	U6 Snrna-associated Sm-like Protein Lsm3
e	1	U6 Snrna-associated Sm-like Protein Lsm6
f	1	U6 Snrna-associated Sm-like Protein Lsm5
g	1	U6 Snrna-associated Sm-like Protein Lsm7
h	1	U6 Snrna-associated Sm-like Protein Lsm4
Nucleotides(4 molecules)
C	1	Pre-mrna
D	1	Snr6 Snrna
E	1	Snr14 Snrna
F	1	Snr7-l Snrna
Chemicals and Non-standard biopolymers (2 molecules)
1	1	GUANOSINE-5'-TRIPHOSPHATE
2	1	N,N,7-trimethylguanosine 5'-(trihydrogen diphosphate)

* Click molecule labels to explore molecular sequence information.

Citing MMDB