NIH_MGC_254

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Result Filters

Organism: Rattus norvegicus (Norway rat)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus

Attributes

sex	both
tissue	Brain - Pooled from several tissues from one or more individuals
lab host	DH10B TonA
tissue	brain/CNS
vector	pExpress-1
v_type	plasmid
re_1	EcoRV
re_2	NotI

Description: RNA obtained from brain tissue of 8 wk old animal. Tissues were snap-frozen and kept at -80C before RNA extraction and purification (Tri-reagent method). cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.25kb resulted in an average insert size of 2.18 kb. This primary library is not normalized (normalized library is NIH_MGC_255) and was constructed by Express Genomics (Frederick, MD). Note: this is a NIH_MGC library