3ZXD,3ZX7,3ZXG,5EC5,5GAQ


Conserved Protein Domain Family
PFM_lysenin-like

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cd20225: PFM_lysenin-like 
pore-forming module of lysenin and similar aerolysin-type beta-barrel pore-forming proteins
Lysenin (also known as Efl1) is a sphingomyelin-binding defense protein found in the coelomic fluid of the annelid earthworm Eisenia fetida. This group also contains lysenin-related proteins LRP-1 , LRP-2 , and LRP-3 from Eisenia sp.. They belong to the aerolysin family of beta-pore-forming proteins (beta-PFPs). PFPs are generally secreted as water-soluble monomers, which upon binding to target lipid membranes, oligomerize and form transmembrane pores harmful to cells. Beta-PFPs form pores by transmembrane beta-barrels. Aerolysin-type beta-PFPs are believed to use an amphipathic beta-hairpin to form the beta-barrel, are found in all kingdoms of life and many are bacterial toxins. In addition to having a role in microbial infection, they have potential as biotechnological sensors and delivery systems. They share a similar monomeric architecture, with a variable membrane-binding domain and a structurally conserved pore-forming region. A significant portion of the monomeric subunit structure is re-organized to form the pore. Oligomers formed by members of the aerolysin family include: hepta- (aerolysin), octa- (Dln1), and nonameric oligomers (lysenin and monalysin).
Statistics
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PSSM-Id: 380795
Aligned: 9 rows
Threshold Bit Score: 302.364
Created: 19-Dec-2018
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 57 residues -Click on image for an interactive view with Cn3D
Feature 1:putative membrane-spanning region [structural motif]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                        #############################################
3ZXD_A      7 GYEQIEVDVVAVWKEGYVYENRGSTSVDQKITITKGMKNVNSETRTVTATHSIGSTISTGDAFEIGSVEVSYSHSHEESQ 86  common brandling worm
3ZX7_A      7 GYEQIEVDVVAVWKEGYVYENRGSTSVDQKITITKGXKNVNSETRTVTATHSIGSTISTGDAFEIGSVEVSYSHSHEESQ 86  common brandling worm
3ZXG_A      7 GYEQIEVDVVAVWKEGYVYENRGSTSVDQKITITKGMKNVNSETRTVTATHSIGSTISTGDAFEIGSVEVSYSHSHEESQ 86  common brandling worm
5EC5_A      9 GYEQIEVDVVAVWKEGYVYENRGSTSVDQKITITKGMKNVNSETRTVTATHSIGSTISTGDAFEIGSVEVSYSHSHQKSQ 88  common brandling worm
5GAQ_A      8 GYEQIEVDVVAVWKEGYVYENRGSTSVDQKITITKGMKNVNSETRTVTATHSIGSTISTGDAFEIGSVEVSYSHSHEESQ 87  common brandling worm
O18425     10 GYEQIEVDVVAVWKEGYVYENRGSTSVEQKIKITKGMRNLNSETKTLTASHSIGSTISTGDLFEIATVDVSYSYSHEESQ 89  common brandling worm
Q3LX99     10 GLEEIEVDVVAVWKEGYVYENRGDTSVEQKITMTKGMKNLNSETKTLTATHTVGRTLKVGDPFEIGSVEVSYSFSHQESQ 89  common brandling worm
O18424     10 GFEEIEVDVVSVWKEGYAYENRGNSSVQQKITMTKGMKNLNSETKTLTATHTLGRTLKVGDPFEIASVEVSYTFSHQKSQ 89  common brandling worm
AAB67727   10 GYEQIEVDVVAVWKEGYVYENRGSTSVEQKIKITKGMRNLNSETKTLTASHSIGSTISTGDLFEIATVDVSYSYSHEESQ 89  Eisenia andrei
Feature 1     ############                                                          
3ZXD_A     87 VSMTETEVYESKVIEHTITIPPTSKFTRWQLNADVGGADIEYMYLIDEVTPIGGTQSIPQVITSRAKIIV 156 common brandling worm
3ZX7_A     87 VSXTETEVYESKVIEHTITIPPTSKFTRWQLNADVGGADIEYXYLIDEVTPIGGTQSIPQVITSRAKIIV 156 common brandling worm
3ZXG_A     87 VSMTETEVYESKVIEHTITIPPTSKFTRWQLNADVGGADIEYMYLIDEVTPIGGTQSIPQVITSRAKIIV 156 common brandling worm
5EC5_A     89 VSMTQTEVYSSKVIEHTITIPPTSKFTRWQLNADVGGAGIEYMYLIDEVTPIGGTQSIPQVITSRAKIIV 158 common brandling worm
5GAQ_A     88 VSMTETEVYESKVIEHTITIPPTSKFTRWQLNADVGGADIEYMYLIDEVTPIGGTQSIPQVITSRAKIIV 157 common brandling worm
O18425     90 VSMTETEVYESKEIEHTITIPPTSKFTRWQLNADVGGADIEYMYLIDEVTPIGGTLSIPQVIKSRAKILV 159 common brandling worm
Q3LX99     90 VSMTQTEVYSSQVIEHTVTIPPTSKFTRWKLNADVGGTDIEYMYLIDEVTPISVTQTIPQVIRSRAKILV 159 common brandling worm
O18424     90 VSMTQTEVYSSQVIEHTVTIPPNKKFTRWKLNADVGGTGIEYMYLIDEVTAIGADLTIPEVNKSRAKILV 159 common brandling worm
AAB67727   90 VSMTETEVYESKEIEHTITIPPTSKFTRWQLNADVGGADIEYMYLIDEVTPIGGTLSIPQVIKSRAKILV 159 Eisenia andrei

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