Mylk myosin light chain kinase [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Mylkprovided by RGD
Official Full Name: myosin light chain kinaseprovided by RGD
Primary source: RGD:1310915
See related: EnsemblRapid:ENSRNOG00000002215 AllianceGenome:RGD:1310915
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Predicted to enable enzyme binding activity and myosin light chain kinase activity. Involved in regulation of synaptic vesicle endocytosis. Is active in synapse. Human ortholog(s) of this gene implicated in adult respiratory distress syndrome; asthma; coronary artery disease; megacystis-microcolon-intestinal hypoperistalsis syndrome; and thoracic aortic aneurysm. Orthologous to human MYLK (myosin light chain kinase). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Uterus (RPKM 657.5), Lung (RPKM 530.3) and 8 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 11q22

Exon count:: 36

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	11	NC_086029.1 (79288243..79535450, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	11	NC_051346.1 (65783008..66030239, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	11	NC_005110.4 (69013060..69260039, complement)

Chromosome 11 - NC_086029.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Myosin light chain kinase mediates intestinal barrier dysfunction following simulated microgravity based on proteomic strategy.
Title: Myosin light chain kinase mediates intestinal barrier dysfunction following simulated microgravity based on proteomic strategy.
Ang II is involved in the degradation of myosin light chain kinase in pressure overload-induced cardiac hypertrophy and that this process was mediated by MMP9.
Title: Angiotensin II Facilitates Matrix Metalloproteinase-9-Mediated Myosin Light Chain Kinase Degradation in Pressure Overload-Induced Cardiac Hypertrophy.
The expression levels of Myosin light chain kinase in the pylorus and ileum of the diabetic rats were decreased and the changes of Myosin light chain kinase expression in these tissues were partially reversed after treatment with insulin.
Title: Myosin light chain kinase is involved in the mechanism of gastrointestinal dysfunction in diabetic rats.
In healthy young female rats, MLCK activity is higher and MLCP activity is lower in the bladder relative to the urethra
Title: Modulation of smooth muscle tonus in the lower urinary tract: interplay of myosin light-chain kinase (MLCK) and MLC phosphatase (MLCP).
Results from various lines of investigation suggest that the actin binding properties of MLCK play important roles in determining actin cytoskeletal organization in smooth muscle, particularly in cytoskeletal rearrangement stimulated by PDBu.
Title: Myosin light chain kinase/actin interaction in phorbol dibutyrate-stimulated smooth muscle cells.

Submit: New GeneRIF Correction

General gene information

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Markers

AU049487 (e-PCR)
- UniSTS:145934

AU049255 (e-PCR)
- UniSTS:145703

RH127440 (e-PCR)
- UniSTS:210750

AA851174 (e-PCR)
- UniSTS:261488

d11rat63 (e-PCR)
- UniSTS:120783

BI293815 (e-PCR)
- UniSTS:245729

d11rat94 (e-PCR)
- UniSTS:120803

BM389975 (e-PCR)
- UniSTS:260595

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables calmodulin binding	IEA Inferred from Electronic Annotation more info
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables myosin light chain kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables myosin light chain kinase activity	ISO Inferred from Sequence Orthology more info
enables scaffold protein binding	IEA Inferred from Electronic Annotation more info
enables scaffold protein binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in aorta smooth muscle tissue morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in bleb assembly	ISO Inferred from Sequence Orthology more info
involved_in cellular hypotonic response	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cellular response to calcium ion	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cellular response to potassium ion	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cellular response to xenobiotic stimulus	ISO Inferred from Sequence Orthology more info
involved_in neurotransmitter receptor transport to postsynaptic membrane	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of calcium ion transport	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of wound healing	ISO Inferred from Sequence Orthology more info
involved_in regulation of synaptic vesicle endocytosis	EXP Inferred from Experiment more info	PubMed
involved_in regulation of synaptic vesicle endocytosis	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of synaptic vesicle endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in smooth muscle contraction	ISO Inferred from Sequence Orthology more info
involved_in tonic smooth muscle contraction	IBA Inferred from Biological aspect of Ancestor more info
involved_in tonic smooth muscle contraction	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in actin cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in cell-cell junction	ISO Inferred from Sequence Orthology more info
is_active_in cleavage furrow	IBA Inferred from Biological aspect of Ancestor more info
located_in cleavage furrow	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in lamellipodium	IBA Inferred from Biological aspect of Ancestor more info
located_in lamellipodium	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in stress fiber	IBA Inferred from Biological aspect of Ancestor more info
located_in stress fiber	ISO Inferred from Sequence Orthology more info
is_active_in synapse	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: myosin light chain kinase, smooth muscle

Names: myosin, light polypeptide kinase

NP_001099344.2

EC 2.7.11.18

XP_006248495.1

EC 2.7.11.18

XP_006248496.1

EC 2.7.11.18

XP_038944047.1

EC 2.7.11.18

XP_038944048.1

EC 2.7.11.18

XP_063126447.1

EC 2.7.11.18

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001105874.2 → NP_001099344.2 myosin light chain kinase, smooth muscle

Status: VALIDATED

Source sequence(s)

JAXUCZ010000011

UniProtKB/TrEMBL

A0A8I6A5J5

Related

ENSRNOP00000062274.4, ENSRNOT00000067060.4

Conserved Domains (8) summary

cd00096
Location:183 → 251

Ig; Immunoglobulin domain

smart00408
Location:523 → 586

IGc2; Immunoglobulin C-2 Type

cd00063
Location:1374 → 1465

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

pfam07679
Location:1852 → 1942

I-set; Immunoglobulin I-set domain

cd14191
Location:1504 → 1762

STKc_MLCK1; Catalytic domain of the Serine/Threonine Kinase, Myosin Light Chain Kinase 1

pfam00069
Location:1507 → 1762

Pkinase; Protein kinase domain

pfam16620
Location:255 → 408

23ISL; Unstructured linker between I-set domains 2 and 3 on MYLCK

cl11960
Location:1281 → 1378

Ig; Immunoglobulin domain

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086029.1 Reference GRCr8

Range

79288243..79535450 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006248434.5 → XP_006248496.1 myosin light chain kinase, smooth muscle isoform X2

See identical proteins and their annotated locations for XP_006248496.1

UniProtKB/TrEMBL

A0A8I6AQ53, D3ZFU9

Related

ENSRNOP00000071545.3, ENSRNOT00000085618.3

Conserved Domains (8) summary

cd00096
Location:627 → 695

Ig; Immunoglobulin domain

smart00408
Location:514 → 577

IGc2; Immunoglobulin C-2 Type

cd00063
Location:1365 → 1456

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

pfam07679
Location:1843 → 1933

I-set; Immunoglobulin I-set domain

cd14191
Location:1495 → 1753

STKc_MLCK1; Catalytic domain of the Serine/Threonine Kinase, Myosin Light Chain Kinase 1

pfam00069
Location:1498 → 1753

Pkinase; Protein kinase domain

pfam16620
Location:246 → 399

23ISL; Unstructured linker between I-set domains 2 and 3 on MYLCK

cl11960
Location:1272 → 1369

Ig; Immunoglobulin domain
XM_039088119.2 → XP_038944047.1 myosin light chain kinase, smooth muscle isoform X1

UniProtKB/TrEMBL

A0A8I6A5J5

Conserved Domains (9) summary

cd00063
Location:1374 → 1465

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

cd14191
Location:1504 → 1762

STKc_MLCK1; Catalytic domain of the Serine/Threonine Kinase, Myosin Light Chain Kinase 1

pfam16620
Location:255 → 408

23ISL; Unstructured linker between I-set domains 2 and 3 on MYLCK

pfam07679
Location:165 → 254

I-set; Immunoglobulin I-set domain

cd00096
Location:165 → 168

Ig; Ig strand A [structural motif]

cd05762
Location:1280 → 1378

IgI_8_hMLCK_like; Eighth immunoglobulin (Ig)-like domain of human myosin light-chain kinase (MLCK) and similar protein; member of the I-set of IgSF domains

cd20973
Location:1854 → 1941

IgI_telokin-like; immunoglobulin-like domain of telokin and similar proteins; a member of the I-set of IgSF domains

cd20976
Location:509 → 596

IgI_4_MYLK-like; Fourth Ig-like domain from smooth muscle myosin light chain kinase and similar domains ; a member of the I-set of IgSF domains

cl11960
Location:619 → 708

Ig; Immunoglobulin domain
XM_006248433.5 → XP_006248495.1 myosin light chain kinase, smooth muscle isoform X2

See identical proteins and their annotated locations for XP_006248495.1

UniProtKB/TrEMBL

A0A8I6AQ53, D3ZFU9

Conserved Domains (8) summary

cd00096
Location:627 → 695

Ig; Immunoglobulin domain

smart00408
Location:514 → 577

IGc2; Immunoglobulin C-2 Type

cd00063
Location:1365 → 1456

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

pfam07679
Location:1843 → 1933

I-set; Immunoglobulin I-set domain

cd14191
Location:1495 → 1753

STKc_MLCK1; Catalytic domain of the Serine/Threonine Kinase, Myosin Light Chain Kinase 1

pfam00069
Location:1498 → 1753

Pkinase; Protein kinase domain

pfam16620
Location:246 → 399

23ISL; Unstructured linker between I-set domains 2 and 3 on MYLCK

cl11960
Location:1272 → 1369

Ig; Immunoglobulin domain
XM_039088120.2 → XP_038944048.1 myosin light chain kinase, smooth muscle isoform X3

Conserved Domains (7) summary

cd00063
Location:744 → 835

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

cd14191
Location:874 → 1132

STKc_MLCK1; Catalytic domain of the Serine/Threonine Kinase, Myosin Light Chain Kinase 1

pfam07679
Location:511 → 600

I-set; Immunoglobulin I-set domain

cd00096
Location:6 → 13

Ig; Ig strand B [structural motif]

cd05762
Location:650 → 748

IgI_8_hMLCK_like; Eighth immunoglobulin (Ig)-like domain of human myosin light-chain kinase (MLCK) and similar protein; member of the I-set of IgSF domains

cd20973
Location:1225 → 1312

IgI_telokin-like; immunoglobulin-like domain of telokin and similar proteins; a member of the I-set of IgSF domains

cl11960
Location:2 → 78

Ig; Immunoglobulin domain
XM_063270377.1 → XP_063126447.1 myosin light chain kinase, smooth muscle isoform X4

UniProtKB/TrEMBL

A6IRH8