Pcsk2 proprotein convertase subtilisin/kexin type 2 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Pcsk2provided by RGD
Official Full Name: proprotein convertase subtilisin/kexin type 2provided by RGD
Primary source: RGD:3273
See related: EnsemblRapid:ENSRNOG00000005438 AllianceGenome:RGD:3273
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables serine-type endopeptidase activity. Involved in islet amyloid polypeptide processing. Located in dendrite; perikaryon; and secretory granule. Used to study chronic kidney disease. Human ortholog(s) of this gene implicated in myocardial infarction and type 2 diabetes mellitus. Orthologous to human PCSK2 (proprotein convertase subtilisin/kexin type 2). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Restricted expression toward (RPKM 369.2) See more
Orthologs: human mouse all
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Genomic context

Location:: 3q41

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	3	NC_086021.1 (151333938..151636628)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	3	NC_051338.1 (130880422..131183127)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	3	NC_005102.4 (137618891..137923385)

Chromosome 3 - NC_086021.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The nutrient sensor sirtuin 1 regulates the production of CRH post-translationally by affecting PC2 post-translationally by affecting PC2.
Title: The Nutrient and Energy Sensor Sirt1 Regulates the Hypothalamic-Pituitary-Adrenal (HPA) Axis by Altering the Production of the Prohormone Convertase 2 (PC2) Essential in the Maturation of Corticotropin-releasing Hormone (CRH) from Its Prohormone in Male Rats.
The presence of PC and GOAT in the cells, as well as n-octanoic acid in the culture medium, was necessary to produce n-octanoyl ghrelin.
Title: Production of n-octanoyl-modified ghrelin in cultured cells requires prohormone processing protease and ghrelin O-acyltransferase, as well as n-octanoic acid.
PC2, CPE and preproNPY protein expression levels were compared among mature retina tissue, RGC-5 cells and its differentiated cells; differentiated cells or tissues had higher or highest PC2 activity
Title: PC2/CPE-mediated pro-protein processing in tumor cells and its differentiated cells or tissues.
brain ischemia attenuates PC2 and PC2-mediated neuropeptide processing.
Title: Defective neuropeptide processing and ischemic brain injury: a study on proprotein convertase 2 and its substrate neuropeptide in ischemic brains.
PC1/PC3 and PC2 (specific to neural and endocrine cells) are absent in undifferentiated bone marrow stromal stem cells, their expression is initiated upon the induction of differentiation
Title: Proprotein convertases 1 and 2 (PC1 and PC2) are expressed in neurally differentiated rat bone marrow stromal stem cells (BMSCs).
our data anticipate the possibility that carboxypeptidase-E, prohormone convertase 1, and prohormone convertase 2 might be potential target for the maturation of somatostatin
Title: Immunohistochemical expression and colocalization of somatostatin, carboxypeptidase-E and prohormone convertases 1 and 2 in rat brain.
study demonstrates abundant expression of PC2 mRNA in rat brain and shows that thyroid status regulated PC2 expression in the hypothalamus and cortex [PC2]
Title: Regulation of regional expression in rat brain PC2 by thyroid hormone/characterization of novel negative thyroid hormone response elements in the PC2 promoter.
Leptin regulates hypothalamic Pcsk2 expression and requires prohormone processing into mature peptides.
Title: Regulation of hypothalamic prohormone convertases 1 and 2 and effects on processing of prothyrotropin-releasing hormone.
Expression levels of POMC processing enzymes prohormone convertases 1 and 2 (PC1/PC2) were not significantly affected by high dietary salt intake in either WKY or SP-SHR tissues.
Title: Prevalence of melanocortin system transcripts in rat salt homeostasis endocrine tissues.
The prohormone convertase (PC) enzyme PC1 together with PC2 and PC5 may represent a redundant system to ensure production of biologically active cholecystokinin in rat brain
Title: Distribution and colocalization of cholecystokinin with the prohormone convertase enzymes PC1, PC2, and PC5 in rat brain.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Pcsk2 (e-PCR), detects polymorphism
- UniSTS:532491

M55669 (e-PCR)
- UniSTS:124956

RH94397 (e-PCR)
- UniSTS:119645

RH143576 (e-PCR)
- UniSTS:232822

BE117645 (e-PCR)
- UniSTS:248706

RH136607 (e-PCR)
- UniSTS:210587

RH94811 (e-PCR)
- UniSTS:120057

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables endopeptidase activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables serine-type endopeptidase activity	EXP Inferred from Experiment more info	PubMed
enables serine-type endopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables serine-type endopeptidase activity	IEA Inferred from Electronic Annotation more info
enables serine-type endopeptidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables serine-type endopeptidase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in enkephalin processing	ISO Inferred from Sequence Orthology more info
involved_in insulin processing	ISO Inferred from Sequence Orthology more info
involved_in islet amyloid polypeptide processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nervous system development	ISO Inferred from Sequence Orthology more info
involved_in peptide hormone processing	IBA Inferred from Biological aspect of Ancestor more info
involved_in peptide hormone processing	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within peptide hormone processing	ISO Inferred from Sequence Orthology more info
involved_in peptide hormone processing	ISO Inferred from Sequence Orthology more info
involved_in peptide hormone processing	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein autoprocessing	ISO Inferred from Sequence Orthology more info
involved_in protein processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein processing	ISO Inferred from Sequence Orthology more info
involved_in proteolysis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within proteolysis	ISO Inferred from Sequence Orthology more info
involved_in proteolysis	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in dendrite	IDA Inferred from Direct Assay more info	PubMed
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info
located_in extracellular space	ISO Inferred from Sequence Orthology more info
located_in extracellular space	ISS Inferred from Sequence or Structural Similarity more info
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	ISO Inferred from Sequence Orthology more info
is_active_in neuron projection	IBA Inferred from Biological aspect of Ancestor more info
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in perikaryon	IDA Inferred from Direct Assay more info	PubMed
located_in secretory granule	IDA Inferred from Direct Assay more info	PubMed
is_active_in secretory granule	ISO Inferred from Sequence Orthology more info
located_in secretory granule lumen	TAS Traceable Author Statement more info
located_in transport vesicle	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: neuroendocrine convertase 2

Names: KEX2-like endoprotease 2; NEC 2; PC2; prohormone convertase 2; proprotein convertase 2

NP_036878.1

EC 3.4.21.94

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_012746.2 → NP_036878.1 neuroendocrine convertase 2 precursor

See identical proteins and their annotated locations for NP_036878.1

Status: PROVISIONAL

Source sequence(s)

JAXUCZ010000003

UniProtKB/Swiss-Prot

P28841

UniProtKB/TrEMBL

A0A8I6A2I9, A6K734

Related

ENSRNOP00000007249.3, ENSRNOT00000007249.6

Conserved Domains (4) summary

cd04059
Location:121 → 417

Peptidases_S8_Protein_convertases_Kexins_Furin-like; Peptidase S8 family domain in Protein convertases

pfam00082
Location:157 → 434

Peptidase_S8; Subtilase family

pfam01483
Location:503 → 590

P_proprotein; Proprotein convertase P-domain

pfam16470
Location:32 → 108

S8_pro-domain; Peptidase S8 pro-domain