Usp8 ubiquitin specific peptidase 8 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Usp8provided by RGD
Official Full Name: ubiquitin specific peptidase 8provided by RGD
Primary source: RGD:1304979
See related: EnsemblRapid:ENSRNOG00000010729 AllianceGenome:RGD:1304979
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Predicted to enable K48-linked deubiquitinase activity; K63-linked deubiquitinase activity; and cysteine-type deubiquitinase activity. Involved in cellular response to dexamethasone stimulus; cellular response to nerve growth factor stimulus; and regulation of protein catabolic process at postsynapse, modulating synaptic transmission. Located in early endosome. Is active in glutamatergic synapse and postsynaptic density. Human ortholog(s) of this gene implicated in ACTH-secreting pituitary adenoma. Orthologous to human USP8 (ubiquitin specific peptidase 8). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Kidney (RPKM 272.8), Lung (RPKM 250.5) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 3q36

Exon count:: 20

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	3	NC_086021.1 (134413832..134463040)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	3	NC_051338.1 (113962136..114009683)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	3	NC_005102.4 (119173818..119222499)

Chromosome 3 - NC_086021.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

we identify USP8 as a key enzyme that regulates SHANK3 protein levels in neurons
Title: USP8 Deubiquitinates SHANK3 to Control Synapse Density and SHANK3 Activity-Dependent Protein Levels.
USP8 knockdown increases TrkA half-life, suggesting that the deubiquitinating activity of USP8 promotes TrkA degradation.
Title: The deubiquitinating enzyme UBPy/USP8 interacts with TrkA and inhibits neuronal differentiation in PC12 cells.
Homeostatic downscaling of synaptic strength is accompanied by an increase and decrease in Nedd4-1 and USP8 levels, respectively.
Title: Synaptic strength is bidirectionally controlled by opposing activity-dependent regulation of Nedd4-1 and USP8.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables K48-linked deubiquitinase activity	ISO Inferred from Sequence Orthology more info
enables K63-linked deubiquitinase activity	ISO Inferred from Sequence Orthology more info
enables cysteine-type deubiquitinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables cysteine-type deubiquitinase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in Ras protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within Ras protein signal transduction	ISO Inferred from Sequence Orthology more info
involved_in cellular response to dexamethasone stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to nerve growth factor stimulus	IDA Inferred from Direct Assay more info	PubMed
involved_in endosome organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in endosome organization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within mitotic cytokinesis	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of lysosomal protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of amyloid fibril formation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of canonical Wnt signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in protein K48-linked deubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein K63-linked deubiquitination	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within protein deubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein deubiquitination	ISO Inferred from Sequence Orthology more info
involved_in proteolysis	IEA Inferred from Electronic Annotation more info
involved_in regulation of protein catabolic process at postsynapse, modulating synaptic transmission	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of protein catabolic process at postsynapse, modulating synaptic transmission	IEP Inferred from Expression Pattern more info	PubMed
involved_in regulation of protein catabolic process at postsynapse, modulating synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of protein localization	ISO Inferred from Sequence Orthology more info
involved_in regulation of protein stability	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in acrosomal membrane	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
is_active_in dendritic spine	IBA Inferred from Biological aspect of Ancestor more info
located_in early endosome	IDA Inferred from Direct Assay more info	PubMed
located_in early endosome	ISO Inferred from Sequence Orthology more info
located_in endosome membrane	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in midbody	IBA Inferred from Biological aspect of Ancestor more info
located_in midbody	ISO Inferred from Sequence Orthology more info
is_active_in postsynaptic density	IBA Inferred from Biological aspect of Ancestor more info
is_active_in postsynaptic density	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: ubiquitin carboxyl-terminal hydrolase 8

Names: ubiquitin specific protease 8

NP_001099972.1

EC 3.4.19.12

XP_006234978.1

EC 3.4.19.12

XP_063139398.1

EC 3.4.19.12

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001106502.2 → NP_001099972.1 ubiquitin carboxyl-terminal hydrolase 8

See identical proteins and their annotated locations for NP_001099972.1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000003

UniProtKB/TrEMBL

A0A8I6A1E0, A6HPZ0, D3ZN39

Conserved Domains (5) summary

PTZ00121
Location:453 → 610

PTZ00121; MAEBL; Provisional

pfam00443
Location:740 → 1069

UCH; Ubiquitin carboxyl-terminal hydrolase

pfam00581
Location:193 → 304

Rhodanese; Rhodanese-like domain

pfam06098
Location:80 → 160

Radial_spoke_3; Radial spoke protein 3

pfam08969
Location:6 → 115

USP8_dimer; USP8 dimerization domain
NM_001430101.1 → NP_001417030.1 ubiquitin carboxyl-terminal hydrolase 8

Status: VALIDATED

Source sequence(s)

JAXUCZ010000003

UniProtKB/TrEMBL

A0A8I6A1E0, A6HPZ0, D3ZN39

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086021.1 Reference GRCr8

Range

134413832..134463040

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006234916.5 → XP_006234978.1 ubiquitin carboxyl-terminal hydrolase 8 isoform X1

Related

ENSRNOP00000015124.6, ENSRNOT00000015124.8

Conserved Domains (5) summary

PTZ00121
Location:453 → 610

PTZ00121; MAEBL; Provisional

pfam00443
Location:740 → 1069

UCH; Ubiquitin carboxyl-terminal hydrolase

pfam00581
Location:193 → 304

Rhodanese; Rhodanese-like domain

pfam06098
Location:80 → 160

Radial_spoke_3; Radial spoke protein 3

pfam08969
Location:6 → 115

USP8_dimer; USP8 dimerization domain
XM_063283328.1 → XP_063139398.1 ubiquitin carboxyl-terminal hydrolase 8 isoform X2