Eprs1 glutamyl-prolyl-tRNA synthetase 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Eprs1provided by RGD
Official Full Name: glutamyl-prolyl-tRNA synthetase 1provided by RGD
Primary source: RGD:1310952
See related: EnsemblRapid:ENSRNOG00000002393 AllianceGenome:RGD:1310952
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Eprs
Summary: Predicted to enable several functions, including aminoacyl-tRNA ligase activity; protein homodimerization activity; and zinc ion binding activity. Predicted to be involved in several processes, including cellular response to insulin stimulus; cellular response to type II interferon; and tRNA aminoacylation for protein translation. Predicted to be located in plasma membrane. Predicted to be part of GAIT complex; aminoacyl-tRNA synthetase multienzyme complex; and ribonucleoprotein complex. Predicted to be active in cytosol. Human ortholog(s) of this gene implicated in hypomyelinating leukodystrophy 15. Orthologous to human EPRS1 (glutamyl-prolyl-tRNA synthetase 1). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Liver (RPKM 829.2), Kidney (RPKM 361.3) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 13q26

Exon count:: 35

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	13	NC_086031.1 (99431955..99503510)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	13	NC_051348.1 (96901548..96971966)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	13	NC_005112.4 (103300911..103371651)

Chromosome 13 - NC_086031.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables GTPase binding	ISO Inferred from Sequence Orthology more info
enables RNA stem-loop binding	ISO Inferred from Sequence Orthology more info
enables glutamate-tRNA ligase activity	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables proline-tRNA ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables proline-tRNA ligase activity	ISO Inferred from Sequence Orthology more info
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in cellular response to insulin stimulus	ISO Inferred from Sequence Orthology more info
involved_in cellular response to type II interferon	ISO Inferred from Sequence Orthology more info
involved_in glutamyl-tRNA aminoacylation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of translation	ISO Inferred from Sequence Orthology more info
involved_in prolyl-tRNA aminoacylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in prolyl-tRNA aminoacylation	ISO Inferred from Sequence Orthology more info
involved_in regulation of long-chain fatty acid import into cell	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
part_of GAIT complex	ISO Inferred from Sequence Orthology more info
part_of aminoacyl-tRNA synthetase multienzyme complex	IBA Inferred from Biological aspect of Ancestor more info
part_of aminoacyl-tRNA synthetase multienzyme complex	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
part_of ribonucleoprotein complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: bifunctional glutamate/proline--tRNA ligase

Names: bifunctional aminoacyl-tRNA synthetase; proline--tRNA ligase-like

NP_001019409.1

EC 6.1.1.15

EC 6.1.1.17

XP_006250487.1

EC 6.1.1.15

EC 6.1.1.17

XP_006250488.1

EC 6.1.1.15

EC 6.1.1.17

XP_063128251.1

EC 6.1.1.15

EC 6.1.1.17

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001024238.1 → NP_001019409.1 bifunctional glutamate/proline--tRNA ligase isoform 1

See identical proteins and their annotated locations for NP_001019409.1

Status: PROVISIONAL

Source sequence(s)

AY383705

UniProtKB/TrEMBL

A0A8I5ZY25, Q6TXE9

Related

ENSRNOP00000033323.4, ENSRNOT00000038264.6

Conserved Domains (9) summary

cd10309
Location:92 → 175

GST_C_GluProRS_N; Glutathione S-transferase C-terminal-like, alpha helical domain of bifunctional Glutamyl-Prolyl-tRNA synthetase

PLN02907
Location:30 → 685

PLN02907; glutamate-tRNA ligase

PRK08661
Location:993 → 1486

PRK08661; prolyl-tRNA synthetase; Provisional

cd00778
Location:997 → 1260

ProRS_core_arch_euk; Prolyl-tRNA synthetase (ProRS) class II core catalytic domain. ProRS is a homodimer. It is responsible for the attachment of proline to the 3' OH group of ribose of the appropriate tRNA. This domain is primarily responsible for ATP-dependent formation of ...

cd00862
Location:1266 → 1486

ProRS_anticodon_zinc; ProRS Prolyl-anticodon binding domain, long version found predominantly in eukaryotes and archaea. ProRS belongs to class II aminoacyl-tRNA synthetases (aaRS). This alignment contains the anticodon binding domain, which is responsible for specificity in ...

cd00936
Location:800 → 849

WEPRS_RNA; WEPRS_RNA binding domain. This short RNA-binding domain is found in several higher eukaryote aminoacyl-tRNA synthetases (aaRSs). It is found in multiple copies in eukaryotic bifunctional glutamyl-prolyl-tRNA synthetases (EPRS) in a region that separates ...

pfam00458
Location:727 → 779

WHEP-TRS; WHEP-TRS domain

pfam03950
Location:478 → 655

tRNA-synt_1c_C; tRNA synthetases class I (E and Q), anti-codon binding domain

cl00015
Location:215 → 476

nt_trans; nucleotidyl transferase superfamily
NM_001429565.1 → NP_001416494.1 bifunctional glutamate/proline--tRNA ligase isoform 2

Status: VALIDATED

Source sequence(s)

JAXUCZ010000013

UniProtKB/TrEMBL

A0A0G2JZI2, A0A8I6GJ92
NM_001429566.1 → NP_001416495.1 bifunctional glutamate/proline--tRNA ligase isoform 3

Status: VALIDATED

Source sequence(s)

JAXUCZ010000013

UniProtKB/TrEMBL

A0A0G2JZI2

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086031.1 Reference GRCr8

Range

99431955..99503510

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006250425.5 → XP_006250487.1 bifunctional glutamate/proline--tRNA ligase isoform X1

Related

ENSRNOP00000090936.1, ENSRNOT00000116204.2

Conserved Domains (9) summary

cd10309
Location:74 → 157

GST_C_GluProRS_N; Glutathione S-transferase C-terminal-like, alpha helical domain of bifunctional Glutamyl-Prolyl-tRNA synthetase

PLN02907
Location:6 → 718

PLN02907; glutamate-tRNA ligase

PRK08661
Location:1026 → 1519

PRK08661; prolyl-tRNA synthetase; Provisional

cd00778
Location:1030 → 1293

ProRS_core_arch_euk; Prolyl-tRNA synthetase (ProRS) class II core catalytic domain. ProRS is a homodimer. It is responsible for the attachment of proline to the 3' OH group of ribose of the appropriate tRNA. This domain is primarily responsible for ATP-dependent formation of ...

cd00862
Location:1299 → 1519

ProRS_anticodon_zinc; ProRS Prolyl-anticodon binding domain, long version found predominantly in eukaryotes and archaea. ProRS belongs to class II aminoacyl-tRNA synthetases (aaRS). This alignment contains the anticodon binding domain, which is responsible for specificity in ...

cd00936
Location:833 → 882

WEPRS_RNA; WEPRS_RNA binding domain. This short RNA-binding domain is found in several higher eukaryote aminoacyl-tRNA synthetases (aaRSs). It is found in multiple copies in eukaryotic bifunctional glutamyl-prolyl-tRNA synthetases (EPRS) in a region that separates ...

pfam00458
Location:760 → 812

WHEP-TRS; WHEP-TRS domain

pfam00749
Location:197 → 498

tRNA-synt_1c; tRNA synthetases class I (E and Q), catalytic domain

pfam03950
Location:511 → 688

tRNA-synt_1c_C; tRNA synthetases class I (E and Q), anti-codon binding domain
XM_006250426.5 → XP_006250488.1 bifunctional glutamate/proline--tRNA ligase isoform X2

Related

ENSRNOP00000071057.2, ENSRNOT00000085214.3

Conserved Domains (9) summary

cd10309
Location:74 → 157

GST_C_GluProRS_N; Glutathione S-transferase C-terminal-like, alpha helical domain of bifunctional Glutamyl-Prolyl-tRNA synthetase

PLN02907
Location:6 → 711

PLN02907; glutamate-tRNA ligase

PRK08661
Location:1019 → 1512

PRK08661; prolyl-tRNA synthetase; Provisional

cd00778
Location:1023 → 1286

ProRS_core_arch_euk; Prolyl-tRNA synthetase (ProRS) class II core catalytic domain. ProRS is a homodimer. It is responsible for the attachment of proline to the 3' OH group of ribose of the appropriate tRNA. This domain is primarily responsible for ATP-dependent formation of ...

cd00862
Location:1292 → 1512

ProRS_anticodon_zinc; ProRS Prolyl-anticodon binding domain, long version found predominantly in eukaryotes and archaea. ProRS belongs to class II aminoacyl-tRNA synthetases (aaRS). This alignment contains the anticodon binding domain, which is responsible for specificity in ...

cd00936
Location:826 → 875

WEPRS_RNA; WEPRS_RNA binding domain. This short RNA-binding domain is found in several higher eukaryote aminoacyl-tRNA synthetases (aaRSs). It is found in multiple copies in eukaryotic bifunctional glutamyl-prolyl-tRNA synthetases (EPRS) in a region that separates ...

pfam00458
Location:753 → 805

WHEP-TRS; WHEP-TRS domain

pfam00749
Location:197 → 502

tRNA-synt_1c; tRNA synthetases class I (E and Q), catalytic domain

pfam03950
Location:504 → 681

tRNA-synt_1c_C; tRNA synthetases class I (E and Q), anti-codon binding domain
XM_063272181.1 → XP_063128251.1 bifunctional glutamate/proline--tRNA ligase isoform X3

UniProtKB/TrEMBL

A0A8I6G8C4