Pfkfb2 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Pfkfb2provided by RGD
Official Full Name: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2provided by RGD
Primary source: RGD:3309
See related: EnsemblRapid:ENSRNOG00000004162 AllianceGenome:RGD:3309
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: RH2K; PFK-2
Summary: Enables fructose-2,6-bisphosphate 2-phosphatase activity and kinase binding activity. Involved in several processes, including monocarboxylic acid metabolic process; positive regulation of glucokinase activity; and positive regulation of insulin secretion. Predicted to be located in nucleoplasm. Predicted to be active in cytosol. Orthologous to human PFKFB2 (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Heart (RPKM 122.2), Lung (RPKM 90.4) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 13q13

Exon count:: 21

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	13	NC_086031.1 (44683499..44727135, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	13	NC_051348.1 (42147473..42174699, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	13	NC_005112.4 (47413453..47440682, complement)

Chromosome 13 - NC_086031.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

amino acids stimulate Fru-2,6-P2 synthesis by Akt-dependent PFKFB2 phosphorylation and activation and show how signaling and metabolism are inextricably linked.
Title: Akt-dependent activation of the heart 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB2) isoenzyme by amino acids.
mechanisms downstream of AMP-activated protein kinase activation, involving phosphorylation of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase and GKRP are involved in the ATP-independent inhibition of glucose-induced glucokinase translocation
Title: Inhibition of glucokinase translocation by AMP-activated protein kinase is associated with phosphorylation of both GKRP and 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
Rat islets expressed PFKFB2 lacking the C-terminal phosphorylation sites. PFK-2/FBPase-2 protein rather than its product fructose 2,6-P(2) is the over-riding determinant of glucose-induced insulin secretion through regulation of glucokinase activity
Title: A role for PFK-2/FBPase-2, as distinct from fructose 2,6-bisphosphate, in regulation of insulin secretion in pancreatic beta-cells.
coordinate roles for glucokinase and PFK2 in the elevated hepatic glycolysis in fa/fa Zucker rats
Title: Contributions of glucokinase and phosphofructokinase-2/fructose bisphosphatase-2 to the elevated glycolysis in hepatocytes from Zucker fa/fa rats.

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General gene information

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Markers

D13MGH12 (e-PCR)
- UniSTS:120166

Pfkfb2 (e-PCR), detects polymorphism
- UniSTS:546931

RH144599 (e-PCR)
- UniSTS:233844

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables 6-phosphofructo-2-kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 6-phosphofructo-2-kinase activity	IEA Inferred from Electronic Annotation more info
enables 6-phosphofructo-2-kinase activity	ISO Inferred from Sequence Orthology more info
enables 6-phosphofructo-2-kinase activity	ISS Inferred from Sequence or Structural Similarity more info
enables 6-phosphofructo-2-kinase activity	TAS Traceable Author Statement more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables fructose-2,6-bisphosphate 2-phosphatase activity	IBA Inferred from Biological aspect of Ancestor more info
enables fructose-2,6-bisphosphate 2-phosphatase activity	IDA Inferred from Direct Assay more info	PubMed
enables fructose-2,6-bisphosphate 2-phosphatase activity	IEA Inferred from Electronic Annotation more info
enables fructose-2,6-bisphosphate 2-phosphatase activity	ISO Inferred from Sequence Orthology more info
enables fructose-2,6-bisphosphate 2-phosphatase activity	TAS Traceable Author Statement more info	PubMed
enables kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in carbohydrate phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in fructose 2,6-bisphosphate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in fructose 2,6-bisphosphate metabolic process	IEA Inferred from Electronic Annotation more info
involved_in fructose 2,6-bisphosphate metabolic process	TAS Traceable Author Statement more info	PubMed
involved_in fructose metabolic process	IEA Inferred from Electronic Annotation more info
involved_in glucose catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in glycolytic process	ISO Inferred from Sequence Orthology more info
involved_in glycolytic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in lactate metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of glucokinase activity	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of insulin secretion	IDA Inferred from Direct Assay more info	PubMed
involved_in pyruvate metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in response to glucose	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2

Names: 6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 (heart); 6PF-2-K/Fru-2,6-P2ASE heart-type isozyme; 6PF-2-K/Fru-2,6-P2ase 2; PFK-2/FBPase-2; PFK/FBPase 2; fructose 6-phosphate 2-kinase/fructose 2,6-bisphosphatase

NP_001029136.1

EC 2.7.1.105

EC 3.1.3.46

NP_001029137.1

EC 2.7.1.105

EC 3.1.3.46

NP_536725.2

EC 2.7.1.105

EC 3.1.3.46

XP_006249768.1

EC 2.7.1.105

EC 3.1.3.46

XP_006249769.1

EC 2.7.1.105

EC 3.1.3.46

XP_006249770.1

EC 2.7.1.105

EC 3.1.3.46

XP_006249771.1

EC 2.7.1.105

EC 3.1.3.46

XP_006249772.1

EC 2.7.1.105

EC 3.1.3.46

XP_008767647.1

EC 2.7.1.105

EC 3.1.3.46

XP_038946265.1

EC 2.7.1.105

EC 3.1.3.46

XP_038946266.1

EC 2.7.1.105

EC 3.1.3.46

XP_038946268.1

EC 2.7.1.105

EC 3.1.3.46

XP_038946271.1

EC 2.7.1.105

EC 3.1.3.46

XP_063128095.1

EC 2.7.1.105

EC 3.1.3.46

XP_063128096.1

EC 2.7.1.105

EC 3.1.3.46

XP_063128097.1

EC 2.7.1.105

EC 3.1.3.46

XP_063128099.1

EC 2.7.1.105

EC 3.1.3.46

XP_063128100.1

EC 2.7.1.105

EC 3.1.3.46

XP_063128101.1

EC 2.7.1.105

EC 3.1.3.46

XP_063128102.1

EC 2.7.1.105

EC 3.1.3.46

XP_063128103.1

EC 2.7.1.105

EC 3.1.3.46

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001033964.2 → NP_001029136.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 isoform b

See identical proteins and their annotated locations for NP_001029136.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' end of the coding sequence compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.

Source sequence(s)

JAXUCZ010000013

UniProtKB/TrEMBL

A6IBZ3, R9PXY6

Related

ENSRNOP00000082431.1, ENSRNOT00000117528.2

Conserved Domains (3) summary

pfam00300
Location:255 → 438

His_Phos_1; Histidine phosphatase superfamily (branch 1)

pfam01591
Location:29 → 252

6PF2K; 6-phosphofructo-2-kinase

pfam13671
Location:43 → 198

AAA_33; AAA domain
NM_001033965.2 → NP_001029137.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 isoform a

See identical proteins and their annotated locations for NP_001029137.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).

Source sequence(s)

JAXUCZ010000013

UniProtKB/Swiss-Prot

Q64297, Q9JHL5, Q9JJH4, Q9JJH5

UniProtKB/TrEMBL

A0A8I6GKS2, A6IBZ4

Related

ENSRNOP00000005729.3, ENSRNOT00000005729.7

Conserved Domains (3) summary

pfam00300
Location:255 → 438

His_Phos_1; Histidine phosphatase superfamily (branch 1)

pfam01591
Location:29 → 252

6PF2K; 6-phosphofructo-2-kinase

pfam13671
Location:43 → 198

AAA_33; AAA domain
NM_001429487.1 → NP_001416416.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 isoform 4

Status: VALIDATED

Source sequence(s)

JAXUCZ010000013

UniProtKB/TrEMBL

A6IBZ8, R9PXY6
NM_001429488.1 → NP_001416417.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 isoform 5

Status: VALIDATED

Source sequence(s)

JAXUCZ010000013

UniProtKB/TrEMBL

R9PXY6
NM_001429489.1 → NP_001416418.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 isoform c

Status: VALIDATED

Source sequence(s)

JAXUCZ010000013

UniProtKB/TrEMBL

A6IBZ2, R9PXY6
NM_001429490.1 → NP_001416419.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 isoform c

Status: VALIDATED

Source sequence(s)

JAXUCZ010000013

UniProtKB/TrEMBL

A6IBZ2, R9PXY6
NM_001429491.1 → NP_001416420.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 isoform c

Status: VALIDATED

Source sequence(s)

JAXUCZ010000013

UniProtKB/TrEMBL

A6IBZ2, R9PXY6
NM_001429492.1 → NP_001416421.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 isoform 6

Status: VALIDATED

Source sequence(s)

JAXUCZ010000013

UniProtKB/TrEMBL

A0A8I6GKS2
NM_001429493.1 → NP_001416422.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 isoform 6

Status: VALIDATED

Source sequence(s)

JAXUCZ010000013

UniProtKB/TrEMBL

A0A8I6GKS2
NM_080477.3 → NP_536725.2 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 isoform c

See identical proteins and their annotated locations for NP_536725.2

Status: VALIDATED

Description

Transcript Variant: This variant (3) lacks two alternate in-frame exons in the 3' end of the coding sequence compared to variant 1. The resulting isoform (c) has the same N- and C-termini but is shorter compared to isoform a.

Source sequence(s)

BE113134, DY312091, L27084

UniProtKB/TrEMBL

A6IBZ2, R9PXY6

Related

ENSRNOP00000030819.4, ENSRNOT00000037679.8

Conserved Domains (3) summary

pfam00300
Location:255 → 438

His_Phos_1; Histidine phosphatase superfamily (branch 1)

pfam01591
Location:29 → 252

6PF2K; 6-phosphofructo-2-kinase

pfam13671
Location:43 → 198

AAA_33; AAA domain

RNA

NR_190740.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

JAXUCZ010000013

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086031.1 Reference GRCr8

Range

44683499..44727135 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063272027.1 → XP_063128097.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 isoform X3

UniProtKB/TrEMBL

R9PXY6
XM_039090338.2 → XP_038946266.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 isoform X4

UniProtKB/TrEMBL

A0A8I6GKS2

Conserved Domains (2) summary

pfam00300
Location:266 → 432

His_Phos_1; Histidine phosphatase superfamily (branch 1)

pfam01591
Location:44 → 263

6PF2K; 6-phosphofructo-2-kinase
XM_006249709.5 → XP_006249771.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 isoform X6

Conserved Domains (3) summary

pfam00300
Location:255 → 438

His_Phos_1; Histidine phosphatase superfamily (branch 1)

pfam01591
Location:29 → 252

6PF2K; 6-phosphofructo-2-kinase

pfam13671
Location:43 → 198

AAA_33; AAA domain
XM_063272031.1 → XP_063128101.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 isoform X7
XM_063272030.1 → XP_063128100.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 isoform X6

UniProtKB/TrEMBL

A6IBZ2, R9PXY6
XM_063272026.1 → XP_063128096.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 isoform X2

UniProtKB/TrEMBL

A6IBZ8, R9PXY6
XM_006249708.2 → XP_006249770.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 isoform X2

See identical proteins and their annotated locations for XP_006249770.1

UniProtKB/TrEMBL

A6IBZ8, R9PXY6

Related

ENSRNOP00000044027.4, ENSRNOT00000050859.6

Conserved Domains (3) summary

pfam00300
Location:255 → 438

His_Phos_1; Histidine phosphatase superfamily (branch 1)

pfam01591
Location:29 → 252

6PF2K; 6-phosphofructo-2-kinase

pfam13671
Location:43 → 198

AAA_33; AAA domain
XM_063272029.1 → XP_063128099.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 isoform X5

UniProtKB/TrEMBL

R9PXY6
XM_039090337.2 → XP_038946265.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 isoform X1

UniProtKB/TrEMBL

R9PXY6

Related

ENSRNOP00000060044.4, ENSRNOT00000067445.5

Conserved Domains (2) summary

pfam00300
Location:266 → 432

His_Phos_1; Histidine phosphatase superfamily (branch 1)

pfam01591
Location:44 → 263

6PF2K; 6-phosphofructo-2-kinase
XM_006249707.5 → XP_006249769.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 isoform X2

Conserved Domains (3) summary

pfam00300
Location:255 → 438

His_Phos_1; Histidine phosphatase superfamily (branch 1)

pfam01591
Location:29 → 252

6PF2K; 6-phosphofructo-2-kinase

pfam13671
Location:43 → 198

AAA_33; AAA domain
XM_006249706.5 → XP_006249768.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 isoform X2

See identical proteins and their annotated locations for XP_006249768.1

UniProtKB/TrEMBL

A6IBZ8, R9PXY6

Conserved Domains (3) summary

pfam00300
Location:255 → 438

His_Phos_1; Histidine phosphatase superfamily (branch 1)

pfam01591
Location:29 → 252

6PF2K; 6-phosphofructo-2-kinase

pfam13671
Location:43 → 198

AAA_33; AAA domain
XM_008769425.4 → XP_008767647.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 isoform X5

Conserved Domains (3) summary

pfam00300
Location:219 → 402

His_Phos_1; Histidine phosphatase superfamily (branch 1)

pfam01591
Location:1 → 216

6PF2K; 6-phosphofructo-2-kinase

pfam13671
Location:7 → 162

AAA_33; AAA domain
XM_063272025.1 → XP_063128095.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 isoform X2

UniProtKB/TrEMBL

A6IBZ8, R9PXY6
XM_063272032.1 → XP_063128102.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 isoform X10

UniProtKB/TrEMBL

C9DRP5
XM_039090340.1 → XP_038946268.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 isoform X9

Conserved Domains (2) summary

pfam00300
Location:114 → 280

His_Phos_1; Histidine phosphatase superfamily (branch 1)

cl17190
Location:1 → 111

NK; Nucleoside/nucleotide kinase (NK) is a protein superfamily consisting of multiple families of enzymes that share structural similarity and are functionally related to the catalysis of the reversible phosphate group transfer from nucleoside triphosphates ...
XM_006249710.2 → XP_006249772.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 isoform X8

See identical proteins and their annotated locations for XP_006249772.1

UniProtKB/TrEMBL

C9DRP6

Conserved Domains (2) summary

pfam00300
Location:154 → 337

His_Phos_1; Histidine phosphatase superfamily (branch 1)

cl17190
Location:1 → 151

NK; Nucleoside/nucleotide kinase (NK) is a protein superfamily consisting of multiple families of enzymes that share structural similarity and are functionally related to the catalysis of the reversible phosphate group transfer from nucleoside triphosphates ...
XM_063272033.1 → XP_063128103.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 isoform X10

UniProtKB/TrEMBL

C9DRP5
XM_039090343.2 → XP_038946271.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 isoform X11

Conserved Domains (2) summary

pfam01591
Location:44 → 263

6PF2K; 6-phosphofructo-2-kinase

cl11399
Location:265 → 294

HP; Histidine phosphatase domain found in a functionally diverse set of proteins, mostly phosphatases; contains a His residue which is phosphorylated during the reaction