Ube2n ubiquitin-conjugating enzyme E2N [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Ube2nprovided by RGD
Official Full Name: ubiquitin-conjugating enzyme E2Nprovided by RGD
Primary source: RGD:621096
See related: EnsemblRapid:ENSRNOG00000073414 AllianceGenome:RGD:621096
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables ubiquitin-protein transferase activity. Involved in regulation protein catabolic process at postsynapse. Is active in glutamatergic synapse and postsynapse. Orthologous to human UBE2N (ubiquitin conjugating enzyme E2 N). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Testes (RPKM 481.8), Brain (RPKM 455.2) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 7q13

Exon count:: 3

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	7	NC_086025.1 (32041190..32071252)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	7	NC_051342.1 (30154330..30184373)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	7	NC_005106.4 (36610147..36640190)

Chromosome 7 - NC_086025.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Knockdown or conditional knockout of RNF8 or UBC13 in rodent cerebellar granule neurons robustly increases the number of parallel fiber presynaptic boutons and functional parallel fiber/Purkinje cell synapses. In contrast to the role of nuclear RNF8 in proliferating cells, RNF8 operates in the cytoplasm in neurons to suppress synapse differentiation.
Title: RNF8/UBC13 ubiquitin signaling suppresses synapse formation in the mammalian brain.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH125323 (e-PCR)
- UniSTS:162145

RH142361 (e-PCR)
- UniSTS:231608

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ubiquitin binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin conjugating enzyme activity	IBA Inferred from Biological aspect of Ancestor more info
contributes_to ubiquitin conjugating enzyme activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin conjugating enzyme activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin conjugating enzyme activity	ISS Inferred from Sequence or Structural Similarity more info
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin-protein transferase activator activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin-protein transferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables ubiquitin-protein transferase activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin-protein transferase activity	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
acts_upstream_of DNA double-strand break processing	ISO Inferred from Sequence Orthology more info
involved_in T cell receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in antiviral innate immune response	ISO Inferred from Sequence Orthology more info
involved_in biological_process	ND No biological Data available more info
acts_upstream_of double-strand break repair via homologous recombination	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of TORC1 signaling	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of DNA repair	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of NF-kappaB transcription factor activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of canonical NF-kappaB signal transduction	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of double-strand break repair	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of intracellular signal transduction	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein K63-linked ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in postreplication repair	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of postreplication repair	ISO Inferred from Sequence Orthology more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in protein K63-linked ubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein K63-linked ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein K63-linked ubiquitination	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein monoubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein polyubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein ubiquitination	TAS Traceable Author Statement more info	PubMed
involved_in regulation protein catabolic process at postsynapse	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation protein catabolic process at postsynapse	IEP Inferred from Expression Pattern more info	PubMed
involved_in regulation protein catabolic process at postsynapse	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in ubiquitin-dependent protein catabolic process	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
part_of UBC13-MMS2 complex	ISO Inferred from Sequence Orthology more info
part_of UBC13-MMS2 complex	ISS Inferred from Sequence or Structural Similarity more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
is_active_in postsynapse	IDA Inferred from Direct Assay more info	PubMed
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info
part_of ubiquitin conjugating enzyme complex	ISO Inferred from Sequence Orthology more info
part_of ubiquitin ligase complex	ISO Inferred from Sequence Orthology more info
part_of ubiquitin ligase complex	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: ubiquitin-conjugating enzyme E2 N

Names: E2 ubiquitin-conjugating enzyme N; bendless protein; bendless-like ubiquitin-conjugating enzyme; ubiquitin carrier protein N; ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast); ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13); ubiquitin-protein ligase N

NP_446380.1

EC 2.3.2.23

XP_063118946.1

EC 2.3.2.23

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_053928.2 → NP_446380.1 ubiquitin-conjugating enzyme E2 N

See identical proteins and their annotated locations for NP_446380.1

Status: PROVISIONAL

Source sequence(s)

BC090072

UniProtKB/Swiss-Prot

Q9EQX9

UniProtKB/TrEMBL

A6IG42

Related

ENSRNOP00000108459.1, ENSRNOT00000138953.1

Conserved Domains (1) summary

cl00154
Location:4 → 151

UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...