Cubn cubilin [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Cubnprovided by RGD
Official Full Name: cubilinprovided by RGD
Primary source: RGD:68355
See related: EnsemblRapid:ENSRNOG00000029047 AllianceGenome:RGD:68355
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: IFCR; MGA1; GP280
Summary: Enables cargo receptor activity; hemoglobin binding activity; and identical protein binding activity. Involved in several processes, including cobalamin catabolic process; cobalamin transport; and endocytic hemoglobin import into cell. Located in several cellular components, including brush border membrane; cytoplasmic vesicle; and lysosomal lumen. Part of receptor complex. Human ortholog(s) of this gene implicated in coronary artery disease. Orthologous to human CUBN (cubilin). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Restricted expression toward (RPKM 1569.8) See more
Orthologs: human mouse all
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Genomic context

Location:: 17q12.3

Exon count:: 67

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	17	NC_086035.1 (81293619..81501694, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	17	NC_051352.1 (76385046..76593133, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	17	NC_005116.4 (80584921..80807181, complement)

Chromosome 17 - NC_086035.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Upregulation of Megalin, Cubilin, NGAL mRNA expression in kidney may represent tubular injury and apoptosis in chronic condition of rat diabetic model.
Title: Upregulation of Megalin, Cubilin, NGAL mRNA expression in kidney may represent tubular injury and apoptosis in chronic condition of rat diabetic model.
The inhibition of megalin/cubilin or lysosomal cathepsin B reduced albuminuria-induced NLRP3 inflammasome activation.
Title: Megalin/Cubulin-Lysosome-mediated Albumin Reabsorption Is Involved in the Tubular Cell Activation of NLRP3 Inflammasome and Tubulointerstitial Inflammation.
We conclude that the megalin/cubilin/amnionless/Dab2 complex/es participate in intestinal processes, mainly during the lactation period and that Dab2 may act as an adaptor in intestinal processes mediated by ApoER2 and VLDLR.
Title: Dab2, megalin, cubilin and amnionless receptor complex might mediate intestinal endocytosis in the suckling rat.
used immunocytochemistry and reverse transcription-polymerase chain reaction on laser-captured glomeruli to demonstrate synthesis and expression of cubilin in glomerular podocytes
Title: Cubilin is expressed in rat and human glomerular podocytes.
cubilin and megalin constitute a dual-receptor complex facilitating the function of each other in the inner ear
Title: Cubilin and megalin co-localize in the neonatal inner ear.
Three distinct regions of cubilin bind to megalin and its interactions with both megalin and amnionless are essential for its intracellular stability.
Title: Interactions of cubilin with megalin and the product of the amnionless gene (AMN): effect on its stability.
Significant amounts of megalin and cubilin were present in the glomerular cells in addition to the proximal tubular cells. The quantity of these receptors progressively increased in the glomerulus with age.
Title: Age-related change of endocytic receptors megalin and cubilin in the kidney in rats.
AMN interacts with the EGF domains of cubilin for membrane attachment and export from the endoplasmic reticulum, while extracellular cubilin does apical sorting of the complex in a carbohydrate-dependent manner
Title: Contribution of cubilin and amnionless to processing and membrane targeting of cubilin-amnionless complex.
These data suggest that light chains bind the tandem megalin-cubilin complex.
Title: Light chains are a ligand for megalin.
endocytic receptors megalin and cubilin are involved in renal reabsorption of myoglobin
Title: Renal uptake of myoglobin is mediated by the endocytic receptors megalin and cubilin.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH128210 (e-PCR)
- UniSTS:211518

BM387042 (e-PCR)
- UniSTS:261242

RH124704 (e-PCR)
- UniSTS:161526

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables cargo receptor activity	IEA Inferred from Electronic Annotation more info
enables cargo receptor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables cargo receptor activity	ISO Inferred from Sequence Orthology more info
enables cobalamin binding	IEA Inferred from Electronic Annotation more info
enables hemoglobin binding	IDA Inferred from Direct Assay more info	PubMed
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	IEA Inferred from Electronic Annotation more info
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in cholesterol metabolic process	IEA Inferred from Electronic Annotation more info
involved_in cobalamin catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cobalamin metabolic process	IEA Inferred from Electronic Annotation more info
involved_in cobalamin metabolic process	ISO Inferred from Sequence Orthology more info
involved_in cobalamin transport	IEA Inferred from Electronic Annotation more info
involved_in cobalamin transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cobalamin transport	ISO Inferred from Sequence Orthology more info
involved_in endocytic hemoglobin import into cell	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of localization in cell	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within establishment of localization in cell	ISO Inferred from Sequence Orthology more info
involved_in in utero embryonic development	IDA Inferred from Direct Assay more info	PubMed
involved_in lipoprotein transport	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within lipoprotein transport	ISO Inferred from Sequence Orthology more info
involved_in receptor-mediated endocytosis	IDA Inferred from Direct Assay more info	PubMed
involved_in receptor-mediated endocytosis	IEA Inferred from Electronic Annotation more info
involved_in receptor-mediated endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within receptor-mediated endocytosis	ISO Inferred from Sequence Orthology more info
involved_in response to bacterium	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within response to bacterium	ISO Inferred from Sequence Orthology more info
involved_in response to nutrient	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
located_in Golgi-associated vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in apical part of cell	ISO Inferred from Sequence Orthology more info
located_in apical plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in apical plasma membrane	IEA Inferred from Electronic Annotation more info
located_in apical plasma membrane	ISO Inferred from Sequence Orthology more info
located_in brush border	IEA Inferred from Electronic Annotation more info
located_in brush border	ISO Inferred from Sequence Orthology more info
located_in brush border membrane	IDA Inferred from Direct Assay more info	PubMed
located_in clathrin-coated pit	IDA Inferred from Direct Assay more info	PubMed
located_in clathrin-coated pit	IEA Inferred from Electronic Annotation more info
located_in clathrin-coated pit	ISO Inferred from Sequence Orthology more info
located_in coated vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in endocytic vesicle	IEA Inferred from Electronic Annotation more info
located_in endocytic vesicle	ISO Inferred from Sequence Orthology more info
located_in endocytic vesicle membrane	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in endosome	IEA Inferred from Electronic Annotation more info
located_in endosome	ISO Inferred from Sequence Orthology more info
located_in endosome membrane	IEA Inferred from Electronic Annotation more info
located_in extracellular exosome	IEA Inferred from Electronic Annotation more info
located_in extracellular exosome	ISO Inferred from Sequence Orthology more info
located_in lysosomal lumen	IDA Inferred from Direct Assay more info	PubMed
located_in lysosomal membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in microvillus membrane	IEA Inferred from Electronic Annotation more info
is_active_in microvillus membrane	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
part_of protein-containing complex	IDA Inferred from Direct Assay more info	PubMed
part_of receptor complex	IDA Inferred from Direct Assay more info	PubMed
part_of receptor complex	IEA Inferred from Electronic Annotation more info
part_of receptor complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: cubilin

Names: 460 kDa receptor; glycoprotein 280; intrinsic factor-cobalamin receptor; intrinsic factor-vitamin B12 receptor

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_053332.4 → NP_445784.3 cubilin precursor

Status: VALIDATED

Source sequence(s)

JAXUCZ010000017

UniProtKB/TrEMBL

A0A0G2K9R4, A6JM40

Related

ENSRNOP00000075097.2, ENSRNOT00000090064.3

Conserved Domains (6) summary

smart00179
Location:260 → 301

EGF_CA; Calcium-binding EGF-like domain

cd00041
Location:1620 → 1733

CUB; CUB domain; extracellular domain; present in proteins mostly known to be involved in development; not found in prokaryotes, plants and yeast.

cd00054
Location:432 → 468

EGF_CA; Calcium-binding EGF-like domain, present in a large number of membrane-bound and extracellular (mostly animal) proteins. Many of these proteins require calcium for their biological function and calcium-binding sites have been found to be located at the ...

pfam00431
Location:1048 → 1157

CUB; CUB domain

pfam12947
Location:350 → 387

EGF_3; EGF domain

cd22201
Location:38 → 132

cubilin_NTD; N-terminal domain of cubilin and similar proteins