St6gal1 ST6 beta-galactoside alpha-2,6-sialyltransferase 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: St6gal1provided by RGD
Official Full Name: ST6 beta-galactoside alpha-2,6-sialyltransferase 1provided by RGD
Primary source: RGD:3676
See related: EnsemblRapid:ENSRNOG00000001823 AllianceGenome:RGD:3676
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Siat1
Summary: Enables sialyltransferase activity. Involved in several processes, including negative regulation of macrophage apoptotic process; protein sialylation; and regulation of substrate adhesion-dependent cell spreading. Located in Golgi medial cisterna and Golgi trans cisterna. Used to study high grade glioma. Orthologous to human ST6GAL1 (ST6 beta-galactoside alpha-2,6-sialyltransferase 1). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Liver (RPKM 1020.2), Kidney (RPKM 382.3) and 5 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 11q23

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	11	NC_086029.1 (91031481..91158111, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	11	NC_051346.1 (77526837..77653474, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	11	NC_005110.4 (80927601..80981424, complement)

Chromosome 11 - NC_086029.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Comparisons of the structure with pig ST3GAL1 and a bacterial sialyltransferase revealed a similar positioning of donor, acceptor, and catalytic residues that provide a common structural framework for catalysis
Title: Enzymatic basis for N-glycan sialylation: structure of rat α2,6-sialyltransferase (ST6GAL1) reveals conserved and unique features for glycan sialylation.
ST6Gal-I regulates macrophage apoptosis via alpha2-6 sialylation of the TNFR1 death receptor.
Title: ST6Gal-I regulates macrophage apoptosis via α2-6 sialylation of the TNFR1 death receptor.
rat ST6GAL1 mRNA possess an L exon, suggesting conservation of this regulatory element in rodents
Title: Conservation of the ST6Gal I gene and its expression in the mammary gland.
There are two possible mechanisms for the upregulation of plasma ST6Gal I in hepatopathological situations: one accompanied by acute phase reactions to increase hepatic ST6Gal I expression and the other triggered by oxidative stress in the liver.
Title: Molecular insights into beta-galactoside alpha2,6-sialyltransferase secretion in vivo.
soluble ST6Gal I produced by BACE1 plays, at least in part, a role in the sialylation of soluble glycoproteins
Title: Beta-galactoside alpha2,6-sialyltransferase I cleavage by BACE1 enhances the sialylation of soluble glycoproteins. A novel regulatory mechanism for alpha2,6-sialylation.
Results suggest that ST6Gal I is cleaved initially between Leu(37) and Gln(38) by BACE1, and then the three-amino acid sequence at the NH(2) terminus is removed by exopeptidase(s) before secretion from the cells.
Title: Characterization of alpha 2,6-sialyltransferase cleavage by Alzheimer's beta -secretase (BACE1).
BACE1 cleaves ST6Gal I in vivo and controls the secretion of the E41 form of the ST6Gal I protein
Title: In vivo cleavage of alpha2,6-sialyltransferase by Alzheimer beta-secretase.
The addition of rat alpha 2,6-sialyltransferase in a metabolically engineered baculovirus-infected insect cell system enables successful production of sialylated glycoprotein.
Title: Mammalian glycosyltransferase expression allows sialoglycoprotein production by baculovirus-infected insect cells.

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General gene information

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Markers

Siat1 (e-PCR), detects polymorphism
- UniSTS:261912

RH129723 (e-PCR)
- UniSTS:213018

RH127455 (e-PCR)
- UniSTS:210765

D11Got72 (e-PCR)
- UniSTS:114538

d11mgh7 (e-PCR)
- UniSTS:120747

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables beta-galactoside alpha-2,6-sialyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables beta-galactoside alpha-2,6-sialyltransferase activity	ISO Inferred from Sequence Orthology more info
enables beta-galactoside alpha-2,6-sialyltransferase activity	ISS Inferred from Sequence or Structural Similarity more info
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables sialyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables sialyltransferase activity	IEA Inferred from Electronic Annotation more info
enables sialyltransferase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in N-acetylneuraminate metabolic process	ISO Inferred from Sequence Orthology more info
involved_in N-acetylneuraminate metabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of chemotaxis	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of macrophage apoptotic process	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of mononuclear cell proliferation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein N-linked glycosylation via asparagine	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein N-linked glycosylation via asparagine	ISO Inferred from Sequence Orthology more info
involved_in protein N-linked glycosylation via asparagine	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein glycosylation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within protein glycosylation	ISO Inferred from Sequence Orthology more info
involved_in protein sialylation	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of substrate adhesion-dependent cell spreading	IDA Inferred from Direct Assay more info	PubMed
involved_in response to ethanol	IEP Inferred from Expression Pattern more info	PubMed
involved_in sialylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in sialylation	ISO Inferred from Sequence Orthology more info
involved_in sialylation	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
is_active_in Golgi apparatus	IBA Inferred from Biological aspect of Ancestor more info
located_in Golgi cisterna membrane	IEA Inferred from Electronic Annotation more info
located_in Golgi medial cisterna	IDA Inferred from Direct Assay more info	PubMed
located_in Golgi membrane	ISO Inferred from Sequence Orthology more info
located_in Golgi trans cisterna	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular region	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: beta-galactoside alpha-2,6-sialyltransferase 1

Names: CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1; ST6 beta-galactosamide alpha-2,6-sialyltranferase 1; ST6Gal I; ST6GalI; Sialyltransferase 1 (beta-galactoside alpha-2,6-sialytransferase); Sialyltransferase 1 (beta-galactoside alpha-26-sialytransferase); alpha 2,6-ST 1

NP_001106815.1

EC 2.4.3.1

NP_671738.2

EC 2.4.3.1

XP_006248563.1

EC 2.4.3.1

XP_038943921.1

EC 2.4.3.1

XP_038943922.1

EC 2.4.3.1

XP_038943923.1

EC 2.4.3.1

XP_038943924.1

EC 2.4.3.1

XP_038943925.1

EC 2.4.3.1

XP_063126365.1

EC 2.4.3.1

XP_063126366.1

EC 2.4.3.1

XP_063126367.1

EC 2.4.3.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001113344.1 → NP_001106815.1 beta-galactoside alpha-2,6-sialyltransferase 1 isoform 1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longer isoform (1).

Source sequence(s)

JAXUCZ010000011, M18769

UniProtKB/Swiss-Prot

D4ABR2, G3V680, P13721

Conserved Domains (1) summary

pfam00777
Location:166 → 379

Glyco_transf_29; Glycosyltransferase family 29 (sialyltransferase)
NM_147205.2 → NP_671738.2 beta-galactoside alpha-2,6-sialyltransferase 1 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) has an alternate 5' sequence, as compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus, as compared to isoform 1.

Source sequence(s)

BF418553, JAXUCZ010000011, M83143

UniProtKB/TrEMBL

F2Z3S3

Related

ENSRNOP00000002496.7, ENSRNOT00000002496.9

Conserved Domains (1) summary

pfam00777
Location:63 → 190

Glyco_transf_29; Glycosyltransferase family 29 (sialyltransferase)

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086029.1 Reference GRCr8

Range

91031481..91158111 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039087995.2 → XP_038943923.1 beta-galactoside alpha-2,6-sialyltransferase 1 isoform X1

UniProtKB/Swiss-Prot

D4ABR2, G3V680, P13721

Conserved Domains (1) summary

pfam00777
Location:166 → 379

Glyco_transf_29; Glycosyltransferase family 29 (sialyltransferase)
XM_063270296.1 → XP_063126366.1 beta-galactoside alpha-2,6-sialyltransferase 1 isoform X1

UniProtKB/Swiss-Prot

D4ABR2, G3V680, P13721
XM_039087993.2 → XP_038943921.1 beta-galactoside alpha-2,6-sialyltransferase 1 isoform X1

UniProtKB/Swiss-Prot

D4ABR2, G3V680, P13721

Conserved Domains (1) summary

pfam00777
Location:166 → 379

Glyco_transf_29; Glycosyltransferase family 29 (sialyltransferase)
XM_006248501.3 → XP_006248563.1 beta-galactoside alpha-2,6-sialyltransferase 1 isoform X1

UniProtKB/Swiss-Prot

D4ABR2, G3V680, P13721

Conserved Domains (1) summary

pfam00777
Location:166 → 379

Glyco_transf_29; Glycosyltransferase family 29 (sialyltransferase)
XM_039087996.2 → XP_038943924.1 beta-galactoside alpha-2,6-sialyltransferase 1 isoform X1

UniProtKB/Swiss-Prot

D4ABR2, G3V680, P13721

Conserved Domains (1) summary

pfam00777
Location:166 → 379

Glyco_transf_29; Glycosyltransferase family 29 (sialyltransferase)
XM_039087994.2 → XP_038943922.1 beta-galactoside alpha-2,6-sialyltransferase 1 isoform X1

UniProtKB/Swiss-Prot

D4ABR2, G3V680, P13721

Conserved Domains (1) summary

pfam00777
Location:166 → 379

Glyco_transf_29; Glycosyltransferase family 29 (sialyltransferase)
XM_063270295.1 → XP_063126365.1 beta-galactoside alpha-2,6-sialyltransferase 1 isoform X1

UniProtKB/Swiss-Prot

D4ABR2, G3V680, P13721
XM_063270297.1 → XP_063126367.1 beta-galactoside alpha-2,6-sialyltransferase 1 isoform X1

UniProtKB/Swiss-Prot

D4ABR2, G3V680, P13721
XM_039087997.2 → XP_038943925.1 beta-galactoside alpha-2,6-sialyltransferase 1 isoform X1

UniProtKB/Swiss-Prot

D4ABR2, G3V680, P13721

Conserved Domains (1) summary

pfam00777
Location:166 → 379

Glyco_transf_29; Glycosyltransferase family 29 (sialyltransferase)