Krt8 keratin 8 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Krt8provided by RGD
Official Full Name: keratin 8provided by RGD
Primary source: RGD:2984
See related: EnsemblRapid:ENSRNOG00000009779 AllianceGenome:RGD:2984
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: CYKER; Krt2-8
Summary: Predicted to enable scaffold protein binding activity. Involved in response to hydrostatic pressure and sarcomere organization. Located in keratin filament; myofibril; and sarcolemma. Part of dystrophin-associated glycoprotein complex. Human ortholog(s) of this gene implicated in liver cirrhosis. Orthologous to human KRT8 (keratin 8). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Liver (RPKM 734.6), Kidney (RPKM 613.7) and 3 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 7q36

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	7	NC_086025.1 (135002886..135010339, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	7	NC_051342.1 (133124203..133131656, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	7	NC_005106.4 (143596511..143603745, complement)

Chromosome 7 - NC_086025.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Phosphorylation of KRT8 (keratin 8) by excessive mechanical load-activated PKN (protein kinase N) impairs autophagosome initiation and contributes to disc degeneration.
Title: Phosphorylation of KRT8 (keratin 8) by excessive mechanical load-activated PKN (protein kinase N) impairs autophagosome initiation and contributes to disc degeneration.
Results indicate a keratins K8/K18 intermediate filament (IF) contribution to the cell stiffness-extracellular matrix (ECM) interplay through a modulation of Rho-dependent actin organization.
Title: Keratin 8/18 regulation of cell stiffness-extracellular matrix interplay through modulation of Rho-mediated actin cytoskeleton dynamics.
data imply that CK8/18 overexpression, those cytokeratins complex formation associated w/ histone type 2 H2aa3 upregulation & intermediate filament reorganization may drive neoplastic transformation of GST-P positive foci during rat hepatocarcinogenesis
Title: Cytokeratin 8/18 overexpression and complex formation as an indicator of GST-P positive foci transformation into hepatocellular carcinomas.
cytokeratins 8 and 19 are expressed in adult striated muscle, where they may contribute to the organization of both the myoplasm and sarcolemma
Title: Cloning and characterization of cytokeratins 8 and 19 in adult rat striated muscle. Interaction with the dystrophin glycoprotein complex.
shear stress mediates the phosphorylation of serine residues in K8, leading to the disassembly of intermediate filaments in alveolar epithelial cells
Title: Keratin 8 phosphorylation by protein kinase C delta regulates shear stress-mediated disassembly of keratin intermediate filaments in alveolar epithelial cells.
K8 is a physiological substrate for PKCepsilon, and the phosphorylation at Ser8 and Ser23 transduces, at least in part, TRH-PKCepsilon signaling in pituitary cells
Title: Protein kinase C epsilon phosphorylates keratin 8 at Ser8 and Ser23 in GH4C1 cells stimulated by thyrotropin-releasing hormone.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Krt8 (e-PCR), detects polymorphism
- UniSTS:502649

BI284447 (e-PCR)
- UniSTS:244121

RH125425 (e-PCR)
- UniSTS:162247

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-containing complex binding	IPI Inferred from Physical Interaction more info	PubMed
enables scaffold protein binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within cell differentiation involved in embryonic placenta development	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within extrinsic apoptotic signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within hepatocyte apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in response to hydrostatic pressure	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within response to other organism	ISO Inferred from Sequence Orthology more info
involved_in sarcomere organization	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within tumor necrosis factor-mediated signaling pathway	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in Z disc	IDA Inferred from Direct Assay more info	PubMed
located_in Z disc	ISO Inferred from Sequence Orthology more info
located_in apicolateral plasma membrane	ISO Inferred from Sequence Orthology more info
located_in cell periphery	ISO Inferred from Sequence Orthology more info
located_in cell-cell junction	ISO Inferred from Sequence Orthology more info
located_in costamere	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
part_of dystrophin-associated glycoprotein complex	IDA Inferred from Direct Assay more info	PubMed
located_in intermediate filament	ISO Inferred from Sequence Orthology more info
located_in intermediate filament cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in keratin filament	IDA Inferred from Direct Assay more info	PubMed
located_in keratin filament	IEA Inferred from Electronic Annotation more info
located_in keratin filament	ISO Inferred from Sequence Orthology more info
located_in nuclear matrix	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in sarcolemma	IDA Inferred from Direct Assay more info	PubMed
located_in sarcolemma	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: keratin, type II cytoskeletal 8

Names: CK-8; K8; cytokeratin endo A; cytokeratin-8; keratin 8, type II; keratin complex 2, basic, gene 8; type-II keratin Kb8

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_199370.1 → NP_955402.1 keratin, type II cytoskeletal 8

See identical proteins and their annotated locations for NP_955402.1

Status: PROVISIONAL

Source sequence(s)

AY464139

UniProtKB/Swiss-Prot

Q10758, Q5WPB3

UniProtKB/TrEMBL

A0A8I6G4D8, A6KCS2

Related

ENSRNOP00000029068.3, ENSRNOT00000038480.6

Conserved Domains (3) summary

pfam00038
Location:90 → 401

Filament; Intermediate filament protein

pfam16208
Location:63 → 87

Keratin_2_head; Keratin type II head

cl24005
Location:282 → 358

DUF2570; Protein of unknown function (DUF2570)

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086025.1 Reference GRCr8

Range

135002886..135010339 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039078493.2 → XP_038934421.1 keratin, type II cytoskeletal 8 isoform X1

Conserved Domains (1) summary

pfam00038
Location:22 → 314

Filament; Intermediate filament protein