Doc2b double C2 domain beta [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Doc2bprovided by RGD
Official Full Name: double C2 domain betaprovided by RGD
Primary source: RGD:620519
See related: EnsemblRapid:ENSRNOG00000060054 AllianceGenome:RGD:620519
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables calcium-dependent phospholipid binding activity. Involved in calcium ion-regulated exocytosis of neurotransmitter; calcium-dependent activation of synaptic vesicle fusion; and positive regulation of calcium ion-dependent exocytosis. Located in plasma membrane. Part of SNARE complex. Is active in glutamatergic synapse. Is extrinsic component of synaptic vesicle membrane. Orthologous to human DOC2B (double C2 domain beta). [provided by Alliance of Genome Resources, Jan 2025]
Expression: Biased expression in Adrenal (RPKM 68.2), Brain (RPKM 42.7) and 7 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 10q24

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	10	NC_086028.1 (61126295..61151533, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	10	NC_051345.1 (60628029..60653267, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	10	NC_005109.4 (63921255..63952944, complement)

Chromosome 10 - NC_086028.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

acts directly on membranes and stabilizes hemifusion intermediate in cell-free system
Title: Direct quantitative detection of Doc2b-induced hemifusion in optically trapped membranes.
these data reveal the key determinants of Doc2beta that underlie its function during the slow phase of synaptic transmission.
Title: Structural elements that underlie Doc2β function during asynchronous synaptic transmission.
Mutations that disrupt Ca(2)-binding activity endow Doc2beta with novel functional properties during synaptic transmission.
Title: Mutations that disrupt Ca²⁺-binding activity endow Doc2β with novel functional properties during synaptic transmission.
Doc2b exocytotic functions appear to be distinct from how synaptotagmin-1 promotes synaptic neurotransmitter release.
Title: Doc2b promotes GLUT4 exocytosis by activating the SNARE-mediated fusion reaction in a calcium- and membrane bending-dependent manner.

Submit: New GeneRIF Correction

General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables calcium ion binding	ISO Inferred from Sequence Orthology more info
enables calcium-dependent phospholipid binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables syntaxin binding	IEA Inferred from Electronic Annotation more info
enables syntaxin binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in calcium ion-regulated exocytosis of neurotransmitter	IDA Inferred from Direct Assay more info	PubMed
involved_in calcium ion-regulated exocytosis of neurotransmitter	IEA Inferred from Electronic Annotation more info
involved_in calcium ion-regulated exocytosis of neurotransmitter	ISO Inferred from Sequence Orthology more info
involved_in calcium-dependent activation of synaptic vesicle fusion	IBA Inferred from Biological aspect of Ancestor more info
involved_in calcium-dependent activation of synaptic vesicle fusion	IDA Inferred from Direct Assay more info	PubMed
involved_in calcium-dependent activation of synaptic vesicle fusion	IEA Inferred from Electronic Annotation more info
involved_in calcium-dependent activation of synaptic vesicle fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in calcium-dependent activation of synaptic vesicle fusion	ISO Inferred from Sequence Orthology more info
involved_in exocytosis	IEP Inferred from Expression Pattern more info	PubMed
involved_in neurotransmitter secretion	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of calcium ion-dependent exocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of calcium ion-dependent exocytosis	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of insulin secretion	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of insulin secretion	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of insulin secretion	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of vesicle fusion	IDA Inferred from Direct Assay more info	PubMed
involved_in protein localization	IEA Inferred from Electronic Annotation more info
involved_in protein localization	ISO Inferred from Sequence Orthology more info
involved_in protein localization	ISS Inferred from Sequence or Structural Similarity more info
involved_in spontaneous neurotransmitter secretion	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within spontaneous neurotransmitter secretion	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
part_of SNARE complex	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
is_active_in extrinsic component of synaptic vesicle membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in presynapse	IEA Inferred from Electronic Annotation more info
is_active_in synapse	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: double C2-like domain-containing protein beta

Names: doc2-beta; double C2, beta; double C2-like domains, beta

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_031142.1 → NP_112404.1 double C2-like domain-containing protein beta

Status: PROVISIONAL

Source sequence(s)

U70778

UniProtKB/Swiss-Prot

P70610

UniProtKB/TrEMBL

A0A8I5XVQ2

Related

ENSRNOP00000077032.2, ENSRNOT00000108492.2

Conserved Domains (3) summary

cd04035
Location:127 → 250

C2A_Rabphilin_Doc2; C2 domain first repeat present in Rabphilin and Double C2 domain

cd08384
Location:269 → 401

C2B_Rabphilin_Doc2; C2 domain second repeat present in Rabphilin and Double C2 domain

PHA03247
Location:21 → 86

PHA03247; large tegument protein UL36; Provisional