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    Dffa DNA fragmentation factor subunit alpha [ Rattus norvegicus (Norway rat) ]

    Gene ID: 114214, updated on 9-Dec-2024

    Summary

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    Official Symbol
    Dffaprovided by RGD
    Official Full Name
    DNA fragmentation factor subunit alphaprovided by RGD
    Primary source
    RGD:620334
    See related
    EnsemblRapid:ENSRNOG00000013603 AllianceGenome:RGD:620334
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Rattus norvegicus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
    Also known as
    ICAD-S
    Summary
    Predicted to enable deoxyribonuclease inhibitor activity; protein domain specific binding activity; and protein folding chaperone. Predicted to be involved in chaperone-mediated protein folding; negative regulation of deoxyribonuclease activity; and negative regulation of execution phase of apoptosis. Predicted to act upstream of or within negative regulation of apoptotic DNA fragmentation; positive regulation of apoptotic process; and thymocyte apoptotic process. Predicted to be located in several cellular components, including chromatin; cytosol; and nucleus. Predicted to be part of protein-containing complex. Orthologous to human DFFA (DNA fragmentation factor subunit alpha). [provided by Alliance of Genome Resources, Dec 2024]
    Expression
    Biased expression in Muscle (RPKM 141.5), Heart (RPKM 87.5) and 9 other tissues See more
    Orthologs
    human mouse all
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    Genomic context

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    See Dffa in Genome Data Viewer
    Location:
    5q36
    Exon count:
    5
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCr8 (GCF_036323735.1) 5 NC_086023.1 (164823807..164836729)
    RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 5 NC_051340.1 (159540715..159553639)
    106 previous assembly Rnor_6.0 (GCF_000001895.5) 5 NC_005104.4 (165922893..165935822)

    Chromosome 5 - NC_086023.1Genomic Context describing neighboring genes Neighboring gene castor zinc finger 1 Neighboring gene uncharacterized LOC120103036 Neighboring gene peroxisomal biogenesis factor 14 Neighboring gene centromere protein S Neighboring gene cortistatin

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
    • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
    • BioProject: PRJNA238328
    • Publication: PMID 24510058
    • Analysis date: Mon Jun 6 17:44:12 2016

    Bibliography

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    Related articles in PubMed

    1. Characterization of the rat DNA fragmentation factor 35/Inhibitor of caspase-activated DNase (Short form). The endogenous inhibitor of caspase-dependent DNA fragmentation in neuronal apoptosis. Chen D, et al. J Biol Chem, 2000 Dec 8. PMID 10984476
    2. DNA fragmentation factor 45-deficient cells are more resistant to apoptosis and exhibit different dying morphology than wild-type control cells. Zhang J, et al. J Biol Chem, 1999 Dec 24. PMID 10601318
    3. Fifty C-terminal amino acid residues are necessary for the chaperone activity of DFF45 but not for the inhibition of DFF40. Park HH. BMB Rep, 2009 Nov 30. PMID 19944011
    4. Solution structure of DFF40 and DFF45 N-terminal domain complex and mutual chaperone activity of DFF40 and DFF45. Zhou P, et al. Proc Natl Acad Sci U S A, 2001 May 22. PMID 11371636, Free PMC Article
    5. Endonuclease G is required for early embryogenesis and normal apoptosis in mice. Zhang J, et al. Proc Natl Acad Sci U S A, 2003 Dec 23. PMID 14663139, Free PMC Article

    See all (12) citations in PubMed

    GeneRIFs: Gene References Into Functions

    Pathways from PubChem

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    General gene information

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    Homology

    Gene Ontology Provided by RGD

    Function Evidence Code Pubs
    enables deoxyribonuclease inhibitor activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables protein domain specific binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables protein folding chaperone ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    involved_in apoptotic DNA fragmentation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in chaperone-mediated protein folding ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within negative regulation of apoptotic DNA fragmentation ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in negative regulation of apoptotic DNA fragmentation ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in negative regulation of deoxyribonuclease activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in negative regulation of execution phase of apoptosis IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in negative regulation of execution phase of apoptosis ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within positive regulation of apoptotic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within thymocyte apoptotic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    Component Evidence Code Pubs
    located_in chromatin IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in chromatin ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in cytosol ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in nucleus ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane ISO
    Inferred from Sequence Orthology
    more info
    		  
    part_of protein-containing complex ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    DNA fragmentation factor subunit alpha
    Names
    DNA fragmentation factor, alpha polypeptide
    DNA fragmentation factor, alpha subunit

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001398977.1 → NP_001385906.1  DNA fragmentation factor subunit alpha isoform 1

      Status: VALIDATED

      Source sequence(s)
      JAXUCZ010000005
      Related
      ENSRNOP00000104169.1, ENSRNOT00000158286.1
      Conserved Domains (2) summary
      cd06536
      Location:17 → 96
      CIDE_N_ICAD; CIDE_N domain of ICAD. The CIDE_N (cell death-inducing DFF45-like effector, N-terminal) domain is found at the N-terminus of the CAD nuclease (caspase-activated DNase/DNA fragmentation factor, DFF40) and its inhibitor, ICAD (DFF45). These proteins are ...
      pfam09033
      Location:100 → 263
      DFF-C; DNA Fragmentation factor 45kDa, C terminal domain

    2. NM_053679.3 → NP_446131.2  DNA fragmentation factor subunit alpha isoform 2

      See identical proteins and their annotated locations for NP_446131.2

      Status: VALIDATED

      Source sequence(s)
      JAXUCZ010000005
      UniProtKB/TrEMBL
      F7FCC9, Q498U6, Q9JLT3
      Related
      ENSRNOP00000063043.1, ENSRNOT00000059522.4
      Conserved Domains (2) summary
      cd06536
      Location:17 → 96
      CIDE_N_ICAD; CIDE_N domain of ICAD. The CIDE_N (cell death-inducing DFF45-like effector, N-terminal) domain is found at the N-terminus of the CAD nuclease (caspase-activated DNase/DNA fragmentation factor, DFF40) and its inhibitor, ICAD (DFF45). These proteins are ...
      pfam09033
      Location:100 → 261
      DFF-C; DNA Fragmentation factor 45kDa, C terminal domain

    RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCr8

    Genomic

    1. NC_086023.1 Reference GRCr8

      Range
      164823807..164836729
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein Strain
    Heading Accession and Version

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