Cpb1 carboxypeptidase B1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Cpb1provided by RGD
Official Full Name: carboxypeptidase B1provided by RGD
Primary source: RGD:2391
See related: EnsemblRapid:ENSRNOG00000030904 AllianceGenome:RGD:2391
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Cpb
Summary: Enables carboxypeptidase activity. Predicted to be involved in proteolysis. Predicted to be located in cytoplasmic vesicle. Predicted to be active in extracellular space. Orthologous to human CPB1 (carboxypeptidase B1). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Spleen (RPKM 7904.1) and Liver (RPKM 2563.9) See more
Orthologs: human mouse all
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Genomic context

Location:: 2q24

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	2	NC_086020.1 (104684275..104744824, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	2	NC_051337.1 (102755241..102785628, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	2	NC_005101.4 (105059293..105089682, complement)

Chromosome 2 - NC_086020.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The dephosphorylation of CPB1 induced beta-cell proliferation. We thus surmise that the altered PTM of CPB1 is associated with pancreatic regeneration.
Title: Phosphorylation of carboxypeptidase B1 protein regulates β-cell proliferation.
Mutants C383G, C383A had higher k(cat)/K(m) values which indicated increased catalytic abilities. And both had higher thermal stability.
Title: Increasing the refolding efficiency in vitro by site-directed mutagenesis of Cys383 in rat procarboxypeptidase B.
optimum of procarboxypeptidase B activity was achieved at pH 7-9
Title: The renaturation of procarboxypeptidase B by urea gradient gel filtration and some properties of recombinant carboxypeptidase B.
Data show that specific activity of recombinant CPB is 110u/mg.
Title: [Expression, purification and characterization of rat procarboxypeptidase B in Pichia pastoris].
Distinct bradykinin-processing pathways operate in the perfusates of rat mesenteric/coronary beds, with a substantial participation of a des-Arg(9)-BK-forming enzyme identical to pancreatic CPB.
Title: Carboxypeptidase B and other kininases of the rat coronary and mesenteric arterial bed perfusates.
Nitric oxide production in rat lung microvascular endothelial cells is stimulated more efficiently by arginine released from carboxypeptidase substrates than free arginine.
Title: Carboxypeptidase-mediated enhancement of nitric oxide production in rat lungs and microvascular endothelial cells.

Submit: New GeneRIF Correction

General gene information

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Markers

AI504870 (e-PCR)
- UniSTS:164203

RH134552 (e-PCR)
- UniSTS:217832

d2mgh26 (e-PCR)
- UniSTS:121597

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables carboxypeptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables carboxypeptidase activity	ISO Inferred from Sequence Orthology more info
enables metallocarboxypeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables metallocarboxypeptidase activity	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in biological_process	ND No biological Data available more info
involved_in proteolysis	IBA Inferred from Biological aspect of Ancestor more info
involved_in proteolysis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within proteolysis	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cytoplasmic vesicle	IEA Inferred from Electronic Annotation more info
located_in extracellular region	IEA Inferred from Electronic Annotation more info
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: carboxypeptidase B

Names: carboxypeptidase B1 (tissue); pancreatic carboxypeptidase B1

NP_036665.2

EC 3.4.17.2

XP_063137358.1

EC 3.4.17.2

XP_063137359.1

EC 3.4.17.2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_012533.2 → NP_036665.2 carboxypeptidase B precursor

Status: VALIDATED

Source sequence(s)

AW916086, CF249065, CF249171, JAXUCZ010000002

UniProtKB/Swiss-Prot

P19223

Related

ENSRNOP00000048109.3, ENSRNOT00000043381.5

Conserved Domains (2) summary

cd03871
Location:112 → 410

M14_CPB; Peptidase M14 carboxypeptidase subfamily A/B-like; Carboxypeptidase B subgroup

pfam02244
Location:33 → 102

Propep_M14; Carboxypeptidase activation peptide

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086020.1 Reference GRCr8

Range

104684275..104744824 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063281288.1 → XP_063137358.1 carboxypeptidase B isoform X1

UniProtKB/Swiss-Prot

P19223

UniProtKB/TrEMBL

A6IHD4, D3ZAM3
XM_063281289.1 → XP_063137359.1 carboxypeptidase B isoform X1

UniProtKB/Swiss-Prot

P19223

UniProtKB/TrEMBL

A6IHD4, D3ZAM3