Araf A-Raf proto-oncogene, serine/threonine kinase [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Arafprovided by RGD
Official Full Name: A-Raf proto-oncogene, serine/threonine kinaseprovided by RGD
Primary source: RGD:2148
See related: EnsemblRapid:ENSRNOG00000010838 AllianceGenome:RGD:2148
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Araf1
Summary: Enables MAP kinase kinase kinase activity. Involved in protein phosphorylation. Predicted to be active in cytosol and mitochondrion. Human ortholog(s) of this gene implicated in high grade glioma. Orthologous to human ARAF (A-Raf proto-oncogene, serine/threonine kinase). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Brain (RPKM 202.0), Heart (RPKM 181.8) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: Xq11

Exon count:: 17

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	X	NC_086039.1 (3780932..3845919, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	X	NC_051356.1 (1227392..1292356, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	X	NC_005120.4 (1369534..1391603, complement)

Chromosome X - NC_086039.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AW495444 (e-PCR)
- UniSTS:181280

RH131306 (e-PCR)
- UniSTS:214589

RH141572 (e-PCR)
- UniSTS:223566

UniSTS:224356 (e-PCR)
- UniSTS:224356

Araf (e-PCR), detects polymorphism
- UniSTS:259511

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables 3-phosphoinositide-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables AMP-activated protein kinase activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables DNA-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables MAP kinase kinase kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables MAP kinase kinase kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables MAP kinase kinase kinase activity	TAS Traceable Author Statement more info	PubMed
enables Rho-dependent protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables eukaryotic translation initiation factor 2alpha kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS1 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS121 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AT120 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AXS139 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS14 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS36 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S10 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S28 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S57 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T11 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T3 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T45 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T6 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H4S1 kinase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables ribosomal protein S6 kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in MAPK cascade	IBA Inferred from Biological aspect of Ancestor more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in intracellular signal transduction	TAS Traceable Author Statement more info	PubMed
involved_in negative regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of peptidyl-serine phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein phosphorylation	TAS Traceable Author Statement more info	PubMed
involved_in regulation of TOR signaling	ISO Inferred from Sequence Orthology more info
involved_in regulation of TOR signaling	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of proteasomal ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in regulation of proteasomal ubiquitin-dependent protein catabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in signal transduction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	TAS Traceable Author Statement more info
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: serine/threonine-protein kinase A-Raf

Names: A-Raf proto-oncogene serine/threonine-protein kinase; Raf related protein; Ras-binding protein; proto-oncogene A-Raf; proto-oncogene A-Raf-1; v-raf murine sarcoma 3611 viral oncogene homolog 1; v-raf oncogene homolog 1 (murine sarcoma 3611 virus)

NP_001028835.1

EC 2.7.11.1

NP_071977.1

EC 2.7.11.1

XP_038955949.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001033663.2 → NP_001028835.1 serine/threonine-protein kinase A-Raf isoform 2

See identical proteins and their annotated locations for NP_001028835.1

Status: VALIDATED

Source sequence(s)

AB158253, AI060060, CO572305, DV728203

UniProtKB/TrEMBL

A0A8I5ZXA9, A6JZR7, A6JZR9, A6JZS1, Q6P9W2

Conserved Domains (2) summary

cd01816
Location:20 → 91

Raf_RBD; Ubiquitin domain of Raf serine/threonine kinases

pfam00130
Location:99 → 146

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
NM_022532.2 → NP_071977.1 serine/threonine-protein kinase A-Raf isoform 1

See identical proteins and their annotated locations for NP_071977.1

Status: VALIDATED

Source sequence(s)

AI712552, CO572305, X06942

UniProtKB/Swiss-Prot

P14056

UniProtKB/TrEMBL

A6JZR3, A6JZR6

Related

ENSRNOP00000048890.6, ENSRNOT00000039911.5

Conserved Domains (4) summary

cd01816
Location:20 → 91

Raf_RBD; Ubiquitin domain of Raf serine/threonine kinases

pfam00069
Location:309 → 563

Pkinase; Protein kinase domain

pfam00130
Location:99 → 146

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

cl21453
Location:307 → 571

PKc_like; Protein Kinases, catalytic domain

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086039.1 Reference GRCr8

Range

3780932..3845919 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039100021.2 → XP_038955949.1 serine/threonine-protein kinase A-Raf isoform X1

UniProtKB/TrEMBL

A6JZR2, A6JZR3

Conserved Domains (3) summary

cd17133
Location:56 → 128

RBD_ARAF; Ras-binding domain (RBD) found in serine/threonine-protein kinase ARAF

cd20870
Location:133 → 184

C1_A_C-Raf; protein kinase C conserved region 1 (C1 domain) found in A- and C-Raf (Rapidly Accelerated Fibrosarcoma) kinases, and similar proteins

cl21453
Location:344 → 608

PKc_like; Protein Kinases, catalytic domain