Rbm20 RNA binding motif protein 20 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Rbm20provided by RGD
Official Full Name: RNA binding motif protein 20provided by RGD
Primary source: RGD:1307427
See related: EnsemblRapid:ENSRNOG00000014705 AllianceGenome:RGD:1307427
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables pre-mRNA intronic binding activity. Involved in heart formation; negative regulation of mRNA splicing, via spliceosome; and regulation of alternative mRNA splicing, via spliceosome. Located in cytoplasmic ribonucleoprotein granule. Is active in nucleus. Human ortholog(s) of this gene implicated in dilated cardiomyopathy and dilated cardiomyopathy 1DD. Orthologous to human RBM20 (RNA binding motif protein 20). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Heart (RPKM 362.4) and Muscle (RPKM 117.0) See more
Orthologs: human mouse all
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Genomic context

Location:: 1q55

Exon count:: 21

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	1	NC_086019.1 (262671311..262912551)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	1	NC_051336.1 (252683760..252907465)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	1	NC_005100.4 (274391932..274589816)

Chromosome 1 - NC_086019.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Rbm20 ablation is associated with changes in the expression of titin-interacting and metabolic proteins.
Title: Rbm20 ablation is associated with changes in the expression of titin-interacting and metabolic proteins.
RBM20 phosphorylation and its role in nucleocytoplasmic transport and cardiac pathogenesis.
Title: RBM20 phosphorylation and its role in nucleocytoplasmic transport and cardiac pathogenesis.
RBM20-Mediated Pre-mRNA Splicing Has Muscle-Specificity and Differential Hormonal Responses between Muscles and in Muscle Cell Cultures.
Title: RBM20-Mediated Pre-mRNA Splicing Has Muscle-Specificity and Differential Hormonal Responses between Muscles and in Muscle Cell Cultures.
Megaesophagus Is a Major Pathological Condition in Rats With a Large Deletion in the Rbm20 Gene.
Title: Megaesophagus Is a Major Pathological Condition in Rats With a Large Deletion in the Rbm20 Gene.
RBM20 specifically regulates the inclusion of exon 9* in CACNA1C mRNA, resulting in reduced cell-surface membrane expression of l-channels in cardiomyocytes.
Title: RBM20 Regulates CaV1.2 Surface Expression by Promoting Exon 9* Inclusion of CACNA1C in Neonatal Rat Cardiomyocytes.
RBM20 does not regulate the Z- and M-band TTN splicing in the heart, but does in skeletal muscles.
Title: Z-band and M-band titin splicing and regulation by RNA binding motif 20 in striated muscles.
Calcium handling channel proteins are affected and regulated by RBM20.
Title: Splicing Factor RBM20 Regulates Transcriptional Network of Titin Associated and Calcium Handling Genes in The Heart.
These findings reveal a major signal transduction pathway for insulin-induced titin alternative splicing, and place RBM20 in a central position in the pathway, which is consistent with the reputed role of RBM20 in titin alternative splicing.
Title: Insulin regulates titin pre-mRNA splicing through the PI3K-Akt-mTOR kinase axis in a RBM20-dependent manner.
results suggest that, in healthy conditions, RBM20 and RBM24 cooperate to promote the expression of short ENH isoforms.
Title: RBM20 and RBM24 cooperatively promote the expression of short enh splice variants.
combined transcriptome-wide crosslinking immunoprecipitation, RNA-seq, and quantitative proteomics in cell culture and hearts to examine how RBM20 regulates alternative splicing in the heart
Title: RNA-binding protein RBM20 represses splicing to orchestrate cardiac pre-mRNA processing.

Submit: New GeneRIF Correction See all GeneRIFs (12)

General gene information

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Markers

RH141597 (e-PCR)
- UniSTS:223591

RH143918 (e-PCR)
- UniSTS:233164

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables RNA binding	ISO Inferred from Sequence Orthology more info
enables RNA binding	ISS Inferred from Sequence or Structural Similarity more info
enables mRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables pre-mRNA intronic binding	IDA Inferred from Direct Assay more info	PubMed
enables pre-mRNA intronic binding	ISO Inferred from Sequence Orthology more info
enables splicing factor binding	ISO Inferred from Sequence Orthology more info
enables splicing factor binding	ISS Inferred from Sequence or Structural Similarity more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in heart formation	IDA Inferred from Direct Assay more info	PubMed
involved_in heart formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in heart formation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of mRNA splicing, via spliceosome	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of mRNA splicing, via spliceosome	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of mRNA splicing, via spliceosome	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of RNA splicing	ISO Inferred from Sequence Orthology more info
involved_in regulation of RNA splicing	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of RNA splicing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of alternative mRNA splicing, via spliceosome	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of alternative mRNA splicing, via spliceosome	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of mRNA splicing, via spliceosome	ISO Inferred from Sequence Orthology more info
involved_in spliceosome-depend formation of circular RNA	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cytoplasmic ribonucleoprotein granule	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasmic ribonucleoprotein granule	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: RNA-binding protein 20

Names: probable RNA-binding protein 20

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001107611.2 → NP_001101081.2 RNA-binding protein 20

See identical proteins and their annotated locations for NP_001101081.2

Status: PROVISIONAL

Source sequence(s)

EU562301

UniProtKB/Swiss-Prot

C1IEE3, E9PT37

Related

ENSRNOP00000051570.2, ENSRNOT00000054685.4

Conserved Domains (2) summary

smart00451
Location:1139 → 1173

ZnF_U1; U1-like zinc finger

cd12685
Location:521 → 596

RRM_RBM20; RNA recognition motif of vertebrate RNA-binding protein 20 (RBM20)

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086019.1 Reference GRCr8

Range

262671311..262912551

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063265327.1 → XP_063121397.1 RNA-binding protein 20 isoform X2

UniProtKB/TrEMBL

A0A8I6AGI3
XM_063265322.1 → XP_063121392.1 RNA-binding protein 20 isoform X1

UniProtKB/Swiss-Prot

C1IEE3, E9PT37
XM_039080732.2 → XP_038936660.1 RNA-binding protein 20 isoform X3

UniProtKB/Swiss-Prot

C1IEE3, E9PT37

Conserved Domains (3) summary

smart00451
Location:1139 → 1171

ZnF_U1; U1-like zinc finger

pfam15440
Location:635 → 845

THRAP3_BCLAF1; THRAP3/BCLAF1 family

cd12685
Location:521 → 596

RRM_RBM20; RNA recognition motif (RRM) found in vertebrate RNA-binding protein 20 (RBM20)
XM_063265325.1 → XP_063121395.1 RNA-binding protein 20 isoform X2

UniProtKB/TrEMBL

A0A8I6AGI3
XM_039080742.2 → XP_038936670.2 RNA-binding protein 20 isoform X2

UniProtKB/TrEMBL

A0A8I6AGI3
XM_017589306.3 → XP_017444795.2 RNA-binding protein 20 isoform X2

UniProtKB/TrEMBL

A0A8I6AGI3
XM_063265334.1 → XP_063121404.1 RNA-binding protein 20 isoform X4
XM_039080744.2 → XP_038936672.2 RNA-binding protein 20 isoform X4