Arhgap35 Rho GTPase activating protein 35 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Arhgap35provided by RGD
Official Full Name: Rho GTPase activating protein 35provided by RGD
Primary source: RGD:1308738
See related: EnsemblRapid:ENSRNOG00000015852 AllianceGenome:RGD:1308738
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Grlf1; p190RhoGAP
Summary: Enables GTPase activating protein binding activity and GTPase activator activity. Involved in positive regulation of GTPase activity. Predicted to be located in actin cytoskeleton; ciliary basal body; and cytoplasm. Predicted to be active in cytosol. Orthologous to human ARHGAP35 (Rho GTPase activating protein 35). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Kidney (RPKM 259.7), Adrenal (RPKM 224.6) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 1q21

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	1	NC_086019.1 (86330566..86447414, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	1	NC_051336.1 (77202436..77319298, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	1	NC_005100.4 (78456409..78573374, complement)

Chromosome 1 - NC_086019.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Results find that N-GTPase adopts an extended GTPase-like fold with six unique inserts. It bounds constitutively to GTP/Mg2+ and lacks intrinsic catalytic activity. Mutational analysis shows that GTP and Mg2+ binding stabilizes the domain. This data supports both that p190RhoGAP N-GTPase is a nucleotide binding, non-hydrolyzing, pseudoGTPase domain that cannot bind canonical ligands, acting as a protein-protein module.
Title: The N-Terminal GTPase Domain of p190RhoGAP Proteins Is a PseudoGTPase.
the molecular processes responsible for p190A targeting to actin protrusions, were studied.
Title: Cancer-associated mutations in the protrusion-targeting region of p190RhoGAP impact tumor cell migration.
RACK1 promotes adhesion-mediated activation of ERK2 that in turn inhibits p190A-RhoGAP signaling by reducing the peripheral localization of p190A-RhoGAP.
Title: Symmetry breaking in spreading RAT2 fibroblasts requires the MAPK/ERK pathway scaffold RACK1 that integrates FAK, p190A-RhoGAP and ERK2 signaling.
Results demonstrate that Tks5 plays a central role in the recruitment of AFAP-110, p190RhoGAP, and cortactin to drive podosome formation.
Title: Tks5 recruits AFAP-110, p190RhoGAP, and cortactin for podosome formation.
p190-RhoGAP is crucially involved in semaphorin signalling to the actin cytoskeleton, via interaction with plexins.
Title: p190 Rho-GTPase activating protein associates with plexins and it is required for semaphorin signalling.
Results identify p190A RhoGAP as a major target for ERK signaling in adhesion assembly and identify roles for ERK phosphorylation of the C terminus in p190A localization and activity.
Title: Extracellular signal-regulated kinase promotes Rho-dependent focal adhesion formation by suppressing p190A RhoGAP.
Findings suggest that while PKCdelta can regulate p190 activity, possibly at the FF and/or GTPase domains.
Title: PKCdelta influences p190 phosphorylation and activity: events independent of PKCdelta-mediated regulation of endothelial cell stress fiber and focal adhesion formation and barrier function.
These results define a role for PAR-6 and aPKC in dendritic spine biogenesis and maintenance, and reveal an unexpected link between the PAR-6/aPKC complex and RhoA activity.
Title: The PAR-6 polarity protein regulates dendritic spine morphogenesis through p190 RhoGAP and the Rho GTPase.
These results demonstrate that integrin signaling through Arg activates p190 by promoting its association with p120, resulting in recruitment of p190 to the cell periphery where it inhibits Rho.
Title: Integrin signaling through Arg activates p190RhoGAP by promoting its binding to p120RasGAP and recruitment to the membrane.
enhanced sensitivity of the spontaneously hypertensive rat to glucocorticoids and aldosterone may be in part associated with enhanced glucocorticoid and mineralocorticoid receptor densities in the microcirculation
Title: Enhancement of glucocorticoid and mineralocorticoid receptor density in the microcirculation of the spontaneously hypertensive rat.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH135367 (e-PCR)
- UniSTS:218644

RH134350 (e-PCR)
- UniSTS:217632

WI-9140 (e-PCR)
- UniSTS:14425

BF405601 (e-PCR)
- UniSTS:247944

RH132160 (e-PCR)
- UniSTS:215443

RH139289 (e-PCR)
- UniSTS:221375

BE110269 (e-PCR)
- UniSTS:245222

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTPase activating protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables GTPase activator activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activator activity	IDA Inferred from Direct Assay more info	PubMed
enables GTPase activator activity	ISO Inferred from Sequence Orthology more info
enables GTPase activator activity	ISS Inferred from Sequence or Structural Similarity more info
enables GTPase activity	IEA Inferred from Electronic Annotation more info
enables phospholipid binding	ISO Inferred from Sequence Orthology more info
enables phospholipid binding	ISS Inferred from Sequence or Structural Similarity more info
enables protein-containing complex binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in Rho protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of Rho protein signal transduction	ISO Inferred from Sequence Orthology more info
involved_in axon guidance	ISO Inferred from Sequence Orthology more info
involved_in axon guidance	ISS Inferred from Sequence or Structural Similarity more info
involved_in axonal fasciculation	ISO Inferred from Sequence Orthology more info
involved_in axonal fasciculation	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within camera-type eye development	ISO Inferred from Sequence Orthology more info
involved_in cell migration	ISO Inferred from Sequence Orthology more info
involved_in cell migration	ISS Inferred from Sequence or Structural Similarity more info
involved_in central nervous system neuron axonogenesis	ISO Inferred from Sequence Orthology more info
involved_in central nervous system neuron axonogenesis	ISS Inferred from Sequence or Structural Similarity more info
involved_in establishment or maintenance of actin cytoskeleton polarity	ISO Inferred from Sequence Orthology more info
involved_in establishment or maintenance of actin cytoskeleton polarity	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within forebrain development	ISO Inferred from Sequence Orthology more info
involved_in mammary gland development	ISO Inferred from Sequence Orthology more info
involved_in mammary gland development	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within negative regulation of Rho protein signal transduction	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of vascular permeability	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within neural tube closure	ISO Inferred from Sequence Orthology more info
involved_in neuron projection guidance	ISO Inferred from Sequence Orthology more info
involved_in neuron projection guidance	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of GTPase activity	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of cilium assembly	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cilium assembly	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within positive regulation of neuron projection development	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of neuron projection development	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of neuron projection development	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of actin cytoskeleton organization	ISO Inferred from Sequence Orthology more info
involved_in regulation of actin cytoskeleton organization	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of actin polymerization or depolymerization	ISO Inferred from Sequence Orthology more info
involved_in regulation of actin polymerization or depolymerization	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of axonogenesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of axonogenesis	ISO Inferred from Sequence Orthology more info
involved_in regulation of axonogenesis	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within regulation of cell shape	ISO Inferred from Sequence Orthology more info
involved_in regulation of cell size	IBA Inferred from Biological aspect of Ancestor more info
involved_in wound healing, spreading of cells	ISO Inferred from Sequence Orthology more info
involved_in wound healing, spreading of cells	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
located_in actin cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in ciliary basal body	ISO Inferred from Sequence Orthology more info
located_in ciliary basal body	ISS Inferred from Sequence or Structural Similarity more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: rho GTPase-activating protein 35

Names: GAP-associated protein p190; glucocorticoid receptor DNA-binding factor 1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001271132.1 → NP_001258061.1 rho GTPase-activating protein 35

See identical proteins and their annotated locations for NP_001258061.1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000001

UniProtKB/Swiss-Prot

A0A0G2KB46, P81128

Related

ENSRNOP00000075649.1, ENSRNOT00000090519.3

Conserved Domains (5) summary

smart00441
Location:431 → 483

FF; Contains two conserved F residues

smart00173
Location:158 → 250

RAS; Ras subfamily of RAS small GTPases

cd04373
Location:1247 → 1431

RhoGAP_p190; RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of p190-like proteins. p190, also named RhoGAP5, plays a role in neuritogenesis and axon branch stability. p190 shows a preference for Rho, over Rac and Cdc42, and consists of an ...

pfam16512
Location:261 → 340

RhoGAP-FF1; p190-A and -B Rho GAPs FF domain

cd22221
Location:594 → 765

pseudoGTPaseD_p190RhoGAP-A; pseudoGTPase domain found in p190RhoGAP-A and similar proteins

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086019.1 Reference GRCr8

Range

86330566..86447414 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_008758919.3 → XP_008757141.1 rho GTPase-activating protein 35 isoform X1

See identical proteins and their annotated locations for XP_008757141.1

UniProtKB/Swiss-Prot

A0A0G2KB46, P81128

Conserved Domains (5) summary

smart00441
Location:431 → 483

FF; Contains two conserved F residues

smart00173
Location:158 → 250

RAS; Ras subfamily of RAS small GTPases

cd04373
Location:1247 → 1431

RhoGAP_p190; RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of p190-like proteins. p190, also named RhoGAP5, plays a role in neuritogenesis and axon branch stability. p190 shows a preference for Rho, over Rac and Cdc42, and consists of an ...

pfam16512
Location:261 → 340

RhoGAP-FF1; p190-A and -B Rho GAPs FF domain

cd22221
Location:594 → 765

pseudoGTPaseD_p190RhoGAP-A; pseudoGTPase domain found in p190RhoGAP-A and similar proteins
XM_039110038.2 → XP_038965966.1 rho GTPase-activating protein 35 isoform X1

UniProtKB/Swiss-Prot

A0A0G2KB46, P81128

Conserved Domains (5) summary

smart00441
Location:431 → 483

FF; Contains two conserved F residues

smart00173
Location:158 → 250

RAS; Ras subfamily of RAS small GTPases

cd04373
Location:1247 → 1431

RhoGAP_p190; RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of p190-like proteins. p190, also named RhoGAP5, plays a role in neuritogenesis and axon branch stability. p190 shows a preference for Rho, over Rac and Cdc42, and consists of an ...

pfam16512
Location:261 → 340

RhoGAP-FF1; p190-A and -B Rho GAPs FF domain

cd22221
Location:594 → 765

pseudoGTPaseD_p190RhoGAP-A; pseudoGTPase domain found in p190RhoGAP-A and similar proteins
XM_063288062.1 → XP_063144132.1 rho GTPase-activating protein 35 isoform X1

UniProtKB/Swiss-Prot

A0A0G2KB46, P81128
XM_063288066.1 → XP_063144136.1 rho GTPase-activating protein 35 isoform X1

UniProtKB/Swiss-Prot

A0A0G2KB46, P81128