Gckr glucokinase regulator [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Gckrprovided by RGD
Official Full Name: glucokinase regulatorprovided by RGD
Primary source: RGD:2671
See related: EnsemblRapid:ENSRNOG00000048874 AllianceGenome:RGD:2671
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: GKRP; GLRE
Summary: Enables several functions, including glucose sensor activity; kinase binding activity; and kinase inhibitor activity. Involved in negative regulation of hexokinase activity; protein import into nucleus; and response to hexose. Located in cytoplasm and nucleoplasm. Human ortholog(s) of this gene implicated in end stage renal disease and type 2 diabetes mellitus. Orthologous to human GCKR (glucokinase regulator). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Restricted expression toward (RPKM 878.5) See more
Orthologs: human mouse all
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Genomic context

Location:: 6q14

Exon count:: 19

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	6	NC_086024.1 (30765071..30795627, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	6	NC_051341.1 (25044592..25075834, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	6	NC_005105.4 (26355296..26385761, complement)

Chromosome 6 - NC_086024.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

When GK is pre-incubated with glucose and compound A, the inhibition observed by GKRP is time dependent, but independent of GKRP concentration, reflecting the GKA-controlled transition between closed and open GK conformations.
Title: Modulation of glucokinase by glucose, small-molecule activator and glucokinase regulatory protein: steady-state kinetic and cell-based analysis.
Our findings indicate that both glucokinase and glucokinase regulatory proteins may be functionally active before birth and that they can act within a glucose sensor system involved in controlling food intake.
Title: Glucokinase and glucokinase regulatory proteins are functionally coexpressed before birth in the rat brain.
Prototypical activator of Glucokinase (GK) of the amino-heteroaryl-amide type bound to GK in a glucose-dependent manner and impaired the association of GK with GK regulatory protein
Title: Biophysical characterization of the interaction between hepatic glucokinase and its regulatory protein: impact of physiological and pharmacological effectors.
mechanisms downstream of AMP-activated protein kinase activation, involving phosphorylation of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase and GKRP are involved in the ATP-independent inhibition of glucose-induced glucokinase translocation
Title: Inhibition of glucokinase translocation by AMP-activated protein kinase is associated with phosphorylation of both GKRP and 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
The inhibition of glucokinase by rat and human GKRP are compared.
Title: Differences in regulatory properties between human and rat glucokinase regulatory protein.
asparagine-leucine motifs of glucokinase located in the hinge region confer binding to glucokinase regulatory protein (GRP)
Title: Interaction of glucokinase with the liver regulatory protein is conferred by leucine-asparagine motifs of the enzyme.
Substrates and glucagon induced adaptive changes in the mitochondrial glucokinase/GKRP ratio suggesting a regulatory role for GKRP.
Title: Glucokinase regulatory protein is associated with mitochondria in hepatocytes.

Submit: New GeneRIF Correction

General gene information

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Markers

RH129432 (e-PCR)
- UniSTS:212739

RH94779 (e-PCR)
- UniSTS:120026

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables carbohydrate binding	IDA Inferred from Direct Assay more info	PubMed
enables carbohydrate binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables enzyme binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables enzyme binding	IPI Inferred from Physical Interaction more info	PubMed
enables enzyme inhibitor activity	IDA Inferred from Direct Assay more info	PubMed
enables fructose-6-phosphate binding	IBA Inferred from Biological aspect of Ancestor more info
enables fructose-6-phosphate binding	ISO Inferred from Sequence Orthology more info
enables fructose-6-phosphate binding	ISS Inferred from Sequence or Structural Similarity more info
enables glucose sensor activity	IBA Inferred from Biological aspect of Ancestor more info
enables glucose sensor activity	IDA Inferred from Direct Assay more info	PubMed
enables glucose sensor activity	ISO Inferred from Sequence Orthology more info
enables kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables kinase inhibitor activity	IBA Inferred from Biological aspect of Ancestor more info
enables kinase inhibitor activity	IDA Inferred from Direct Assay more info	PubMed
enables kinase inhibitor activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in carbohydrate derivative metabolic process	IEA Inferred from Electronic Annotation more info
involved_in glucose metabolic process	TAS Traceable Author Statement more info	PubMed
involved_in intracellular glucose homeostasis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within intracellular glucose homeostasis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of glucokinase activity	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of glucokinase activity	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of hexokinase activity	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of glucokinase activity	ISO Inferred from Sequence Orthology more info
involved_in protein import into nucleus	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within protein import into nucleus	ISO Inferred from Sequence Orthology more info
involved_in protein localization to nucleus	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within protein localization to nucleus	ISO Inferred from Sequence Orthology more info
involved_in response to fructose	IDA Inferred from Direct Assay more info	PubMed
involved_in response to fructose	ISO Inferred from Sequence Orthology more info
involved_in response to glucose	IDA Inferred from Direct Assay more info	PubMed
involved_in response to glucose	ISO Inferred from Sequence Orthology more info
involved_in triglyceride homeostasis	ISO Inferred from Sequence Orthology more info
involved_in urate metabolic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
is_active_in nucleoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: glucokinase regulatory protein

Names: 68-kDa regulatory protein; glucokinase (hexokinase 4) regulator

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_013120.2 → NP_037252.1 glucokinase regulatory protein

See identical proteins and their annotated locations for NP_037252.1

Status: PROVISIONAL

Source sequence(s)

BC081843

UniProtKB/Swiss-Prot

Q07071

UniProtKB/TrEMBL

A6HA55, X2G4G4

Related

ENSRNOP00000064260.1, ENSRNOT00000073228.4

Conserved Domains (2) summary

PRK05441
Location:293 → 605

murQ; N-acetylmuramic acid-6-phosphate etherase; Reviewed

cl00389
Location:109 → 273

SIS; SIS domain. SIS (Sugar ISomerase) domains are found in many phosphosugar isomerases and phosphosugar binding proteins. SIS domains are also found in proteins that regulate the expression of genes involved in synthesis of phosphosugars.