Atp1a4 ATPase Na+/K+ transporting subunit alpha 4 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Atp1a4provided by RGD
Official Full Name: ATPase Na+/K+ transporting subunit alpha 4provided by RGD
Primary source: RGD:61952
See related: EnsemblRapid:ENSRNOG00000032378 AllianceGenome:RGD:61952
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables P-type sodium:potassium-exchanging transporter activity. Involved in flagellated sperm motility; regulation of cellular pH; and spermatogenesis. Predicted to be located in rod photoreceptor outer segment and sperm midpiece. Predicted to be part of sodium:potassium-exchanging ATPase complex. Predicted to be active in cell projection and plasma membrane. Orthologous to human ATP1A4 (ATPase Na+/K+ transporting subunit alpha 4). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Testes (RPKM 585.2), Muscle (RPKM 104.5) and 1 other tissue See more
Orthologs: human mouse all
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Genomic context

Location:: 13q24

Exon count:: 23

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	13	NC_086031.1 (87216139..87252155, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	13	NC_051348.1 (84683766..84719790, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	13	NC_005112.4 (90605792..90642032, complement)

Chromosome 13 - NC_086031.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Ouabain interactions with the alpha4 isoform of the sodium pump trigger non-classical steroid hormone signaling and integrin expression in spermatocytes.
Title: Ouabain interactions with the α4 isoform of the sodium pump trigger non-classical steroid hormone signaling and integrin expression in spermatogenic cells.
Atp1a4 is tuned for changing extracellular environments during sperm capacitation.
Title: The α4 isoform of the Na⁺,K⁺-ATPase is tuned for changing extracellular environments.
Na,K-ATPase alpha4 activity is up-regulated during sperm capacitation through mechanisms that involve both increases in molecular activity and levels of alpha4 at the sperm plasma membrane.
Title: Activity of the Na,K-ATPase α4 isoform is regulated during sperm capacitation to support sperm motility.
These results provide new genetic evidence for the role of the ATP1A4 isoform in sperm motility, under both noncapacitating and capacitating conditions.
Title: Increased expression of the Na,K-ATPase alpha4 isoform enhances sperm motility in transgenic mice.
In sperm, alpha4 is isoform primarily involved in controlling transmembrane Na+ gradient; alpha4 activity is necessary for maintaining membrane potential, [Ca2+]i, and [H+]i. Thus, alpha4 activity is necessary to maintain sperm motility.
Title: Activity of the Na,K-ATPase alpha4 isoform is important for membrane potential, intracellular Ca2+, and pH to maintain motility in rat spermatozoa.
This isoform is developmentally expressed in haploid and diploid spermatozoa.
Title: Functional expression of the alpha4 isoform of the Na,K-ATPase in both diploid and haploid germ cells of male rats.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Atp1a4 (e-PCR), detects polymorphism
- UniSTS:480321

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATPase-coupled monoatomic cation transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables P-type sodium:potassium-exchanging transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables P-type sodium:potassium-exchanging transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables P-type sodium:potassium-exchanging transporter activity	IEA Inferred from Electronic Annotation more info
enables P-type sodium:potassium-exchanging transporter activity	ISO Inferred from Sequence Orthology more info
enables P-type sodium:potassium-exchanging transporter activity	TAS Traceable Author Statement more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in establishment of localization in cell	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within establishment of localization in cell	ISO Inferred from Sequence Orthology more info
involved_in establishment or maintenance of transmembrane electrochemical gradient	IEA Inferred from Electronic Annotation more info
involved_in fertilization	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within fertilization	ISO Inferred from Sequence Orthology more info
involved_in flagellated sperm motility	IDA Inferred from Direct Assay more info	PubMed
involved_in flagellated sperm motility	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within flagellated sperm motility	ISO Inferred from Sequence Orthology more info
involved_in flagellated sperm motility	ISO Inferred from Sequence Orthology more info
involved_in flagellated sperm motility	ISS Inferred from Sequence or Structural Similarity more info
involved_in intracellular potassium ion homeostasis	IBA Inferred from Biological aspect of Ancestor more info
involved_in intracellular sodium ion homeostasis	IBA Inferred from Biological aspect of Ancestor more info
involved_in potassium ion import across plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
involved_in potassium ion transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in potassium ion transmembrane transport	ISO Inferred from Sequence Orthology more info
involved_in potassium ion transport	TAS Traceable Author Statement more info	PubMed
involved_in proton transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of cellular pH	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of cellular pH	IEA Inferred from Electronic Annotation more info
involved_in regulation of cellular pH	ISO Inferred from Sequence Orthology more info
involved_in regulation of cellular pH	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of membrane potential	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of membrane potential	ISO Inferred from Sequence Orthology more info
involved_in sodium ion export across plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
involved_in sodium ion transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in sodium ion transmembrane transport	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within sodium ion transport	ISO Inferred from Sequence Orthology more info
involved_in sodium ion transport	TAS Traceable Author Statement more info	PubMed
involved_in spermatogenesis	IEA Inferred from Electronic Annotation more info
involved_in spermatogenesis	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within spermatogenesis	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
is_active_in cell projection	IBA Inferred from Biological aspect of Ancestor more info
located_in endomembrane system	IEA Inferred from Electronic Annotation more info
located_in photoreceptor cell cilium	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in rod photoreceptor outer segment	IEA Inferred from Electronic Annotation more info
located_in rod photoreceptor outer segment	ISO Inferred from Sequence Orthology more info
part_of sodium:potassium-exchanging ATPase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of sodium:potassium-exchanging ATPase complex	IEA Inferred from Electronic Annotation more info
part_of sodium:potassium-exchanging ATPase complex	ISO Inferred from Sequence Orthology more info
part_of sodium:potassium-exchanging ATPase complex	TAS Traceable Author Statement more info	PubMed
located_in sperm midpiece	IEA Inferred from Electronic Annotation more info
located_in sperm midpiece	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: sodium/potassium-transporting ATPase subunit alpha-4

Names: ATPase, Na+/K+ transporting, alpha 4 polypeptide; Na(+)/K(+) ATPase alpha-4 subunit; sodium pump subunit alpha-4; sodium/potassium-transporting ATPase subunit alpha-4-like

NP_001257959.1

EC 7.2.2.13

NP_074039.1

EC 7.2.2.13

XP_017454251.1

EC 7.2.2.13

XP_063128272.1

EC 7.2.2.13

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001271030.1 → NP_001257959.1 sodium/potassium-transporting ATPase subunit alpha-4 isoform 1

See identical proteins and their annotated locations for NP_001257959.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) has an alternate splice site in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same protein.

Source sequence(s)

BC087015, JAXUCZ010000013

UniProtKB/Swiss-Prot

Q64541

UniProtKB/TrEMBL

Q5PQV3

Conserved Domains (1) summary

cd02608
Location:67 → 1025

P-type_ATPase_Na-K_like; alpha-subunit of Na(+)/K(+)-ATPases and of gastric H(+)/K(+)-ATPase, similar to the human Na(+)/K(+)-ATPase alpha subunits 1-4
NM_001429529.1 → NP_001416458.1 sodium/potassium-transporting ATPase subunit alpha-4 isoform 2

Status: VALIDATED

Source sequence(s)

JAXUCZ010000013

UniProtKB/TrEMBL

Q5PQV3
NM_001429530.1 → NP_001416459.1 sodium/potassium-transporting ATPase subunit alpha-4 isoform 3

Status: VALIDATED

Source sequence(s)

JAXUCZ010000013

UniProtKB/TrEMBL

Q5PQV3
NM_001429533.1 → NP_001416462.1 sodium/potassium-transporting ATPase subunit alpha-4 isoform 4

Status: VALIDATED

Source sequence(s)

JAXUCZ010000013

UniProtKB/TrEMBL

Q5PQV3
NM_001429534.1 → NP_001416463.1 sodium/potassium-transporting ATPase subunit alpha-4 isoform 4

Status: VALIDATED

Source sequence(s)

JAXUCZ010000013

UniProtKB/TrEMBL

Q5PQV3
NM_001429536.1 → NP_001416465.1 sodium/potassium-transporting ATPase subunit alpha-4 isoform 5

Status: VALIDATED

Source sequence(s)

JAXUCZ010000013

UniProtKB/TrEMBL

Q5PQV3
NM_001429537.1 → NP_001416466.1 sodium/potassium-transporting ATPase subunit alpha-4 isoform 6

Status: VALIDATED

Source sequence(s)

JAXUCZ010000013

UniProtKB/TrEMBL

Q5PQV3
NM_022848.3 → NP_074039.1 sodium/potassium-transporting ATPase subunit alpha-4 isoform 1

See identical proteins and their annotated locations for NP_074039.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) is the longer transcript. Both variants 1 and 2 encode the same protein.

Source sequence(s)

JAXUCZ010000013

UniProtKB/Swiss-Prot

Q64541

UniProtKB/TrEMBL

Q5PQV3

Related

ENSRNOP00000062079.3, ENSRNOT00000064601.5

Conserved Domains (1) summary

cd02608
Location:67 → 1025

P-type_ATPase_Na-K_like; alpha-subunit of Na(+)/K(+)-ATPases and of gastric H(+)/K(+)-ATPase, similar to the human Na(+)/K(+)-ATPase alpha subunits 1-4

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086031.1 Reference GRCr8

Range

87216139..87252155 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017598762.3 → XP_017454251.1 sodium/potassium-transporting ATPase subunit alpha-4 isoform X1
XM_063272202.1 → XP_063128272.1 sodium/potassium-transporting ATPase subunit alpha-4 isoform X1