Ehmt2 euchromatic histone lysine methyltransferase 2 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Ehmt2provided by RGD
Official Full Name: euchromatic histone lysine methyltransferase 2provided by RGD
Primary source: RGD:1302972
See related: EnsemblRapid:ENSRNOG00000030630 AllianceGenome:RGD:1302972
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: G9a; Bat8; Ng36
Summary: Predicted to enable several functions, including C2H2 zinc finger domain binding activity; p53 binding activity; and protein-lysine N-methyltransferase activity. Involved in several processes, including long-term memory; response to ethanol; and response to fungicide. Located in nucleus. Biomarker of fetal alcohol spectrum disorder. Human ortholog(s) of this gene implicated in idiopathic pulmonary fibrosis. Orthologous to human EHMT2 (euchromatic histone lysine methyltransferase 2). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Adrenal (RPKM 211.8), Kidney (RPKM 174.5) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 20p12

Exon count:: 29

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	20	NC_086038.1 (3924263..3941238, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	20	NC_051355.1 (3919623..3936751, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	20	NC_005119.4 (4576033..4592980)

Chromosome 20 - NC_086038.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

H3K9me2 regulation of BDNF expression via G9a partakes in the progression of heart failure.
Title: H3K9me2 regulation of BDNF expression via G9a partakes in the progression of heart failure.
The mechanism of sevoflurane post-treatment alleviating hypoxic-ischemic encephalopathy by affecting histone methyltransferase G9a in rats.
Title: The mechanism of sevoflurane post-treatment alleviating hypoxic-ischemic encephalopathy by affecting histone methyltransferase G9a in rats.
Histone Methyltransferases SUV39H1 and G9a and DNA Methyltransferase DNMT1 in Penumbra Neurons and Astrocytes after Photothrombotic Stroke.
Title: Histone Methyltransferases SUV39H1 and G9a and DNA Methyltransferase DNMT1 in Penumbra Neurons and Astrocytes after Photothrombotic Stroke.
Expression of G9a in Auditory Cortex Is Downregulated in a Rat Model of Age-Related Hearing Loss.
Title: Expression of G9a in Auditory Cortex Is Downregulated in a Rat Model of Age-Related Hearing Loss.
The Histone Methyltransferase G9a Controls Axon Growth by Targeting the RhoA Signaling Pathway.
Title: The Histone Methyltransferase G9a Controls Axon Growth by Targeting the RhoA Signaling Pathway.
results suggest that regulation of G9a-influenced gene expression could be a common epigenetic mechanism for co-morbid anxiety and psychostimulant addiction.
Title: Knockdown of the histone di-methyltransferase G9a in nucleus accumbens shell decreases cocaine self-administration, stress-induced reinstatement, and anxiety.
we demonstrate that G9a, an epigenetic repressor of gene expression, acting in the nucleus accumbens, a brain reward region, is capable of increasing both addiction- and anxiety-like behaviors in rats
Title: Overexpression of the Histone Dimethyltransferase G9a in Nucleus Accumbens Shell Increases Cocaine Self-Administration, Stress-Induced Reinstatement, and Anxiety.
Epigenetic regulation by G9a/GLP complex ameliorates amyloid-beta induced deficits in long-term plasticity and synaptic tagging/capture in hippocampal pyramidal neurons.
Title: Epigenetic regulation by G9a/GLP complex ameliorates amyloid-beta 1-42 induced deficits in long-term plasticity and synaptic tagging/capture in hippocampal pyramidal neurons.
miR-217-mediated, genetic, or pharmacological inactivation of EHMT1/2 was sufficient to promote pathological hypertrophy
Title: The H3K9 dimethyltransferases EHMT1/2 protect against pathological cardiac hypertrophy.
findings indicate that G9a contributes critically to transcriptional repression of MORs in primary sensory neurons in neuropathic pain. G9a inhibitors may be used to enhance the opioid analgesic effect in the treatment of chronic neuropathic pain.
Title: Nerve Injury Diminishes Opioid Analgesia through Lysine Methyltransferase-mediated Transcriptional Repression of μ-Opioid Receptors in Primary Sensory Neurons.

Submit: New GeneRIF Correction See all GeneRIFs (14)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D17Ertd710e (e-PCR), detects polymorphism
- UniSTS:167224

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables C2H2 zinc finger domain binding	ISO Inferred from Sequence Orthology more info
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables histone H3K27 methyltransferase activity	ISO Inferred from Sequence Orthology more info
enables histone H3K56 methyltransferase activity	ISO Inferred from Sequence Orthology more info
enables histone H3K9 methyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables histone H3K9 methyltransferase activity	ISO Inferred from Sequence Orthology more info
enables p53 binding	ISO Inferred from Sequence Orthology more info
enables promoter-specific chromatin binding	ISO Inferred from Sequence Orthology more info
enables protein-lysine N-methyltransferase activity	ISO Inferred from Sequence Orthology more info
enables transcription corepressor binding	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA methylation-dependent constitutive heterochromatin formation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within behavioral response to cocaine	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cellular response to cocaine	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cellular response to starvation	ISO Inferred from Sequence Orthology more info
involved_in cellular response to xenobiotic stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in epigenetic regulation of gene expression	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within fertilization	ISO Inferred from Sequence Orthology more info
involved_in long-term memory	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in methylation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within negative regulation of autophagosome assembly	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of gene expression via chromosomal CpG island methylation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within neuron fate specification	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within oocyte development	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within organ growth	ISO Inferred from Sequence Orthology more info
involved_in peptidyl-lysine dimethylation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within phenotypic switching	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within protein methylation	ISO Inferred from Sequence Orthology more info
involved_in regulation of DNA replication	ISO Inferred from Sequence Orthology more info
involved_in regulation of protein modification process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in response to alcohol	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to ethanol	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to fungicide	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to nutrient levels	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within spermatid development	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within synaptonemal complex assembly	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in chromatin	IBA Inferred from Biological aspect of Ancestor more info
located_in chromatin	ISO Inferred from Sequence Orthology more info
located_in nuclear speck	IEA Inferred from Electronic Annotation more info
located_in nuclear speck	ISO Inferred from Sequence Orthology more info
NOT located_in nucleolus	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: histone-lysine N-methyltransferase EHMT2

Names: HLA-B associated transcript 8, rat orthologue; euchromatic histone-lysine N-methyltransferase 2; histone-lysine N-methyltransferase, H3 lysine-9 specific 3

NP_997628.1

EC 2.1.1.354

XP_006256016.3

EC 2.1.1.354

XP_006256017.3

EC 2.1.1.354

XP_006256018.3

EC 2.1.1.354

XP_006256019.1

EC 2.1.1.354

XP_006256020.1

EC 2.1.1.354

XP_006256021.1

EC 2.1.1.354

XP_038954735.1

EC 2.1.1.354

XP_063135279.1

EC 2.1.1.354

XP_063135280.1

EC 2.1.1.354

XP_063135281.1

EC 2.1.1.354

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_212463.1 → NP_997628.1 histone-lysine N-methyltransferase EHMT2

See identical proteins and their annotated locations for NP_997628.1

Status: VALIDATED

Source sequence(s)

BX883045

UniProtKB/TrEMBL

A0A8J8XWH8, F1M7S8, Q6MG72

Related

ENSRNOP00000098066.2, ENSRNOT00000120383.2

Conserved Domains (6) summary

PHA03247
Location:5 → 258

PHA03247; large tegument protein UL36; Provisional

COG0666
Location:682 → 874

ANKYR; Ankyrin repeat [Signal transduction mechanisms]

sd00045
Location:838 → 868

ANK; ANK repeat [structural motif]

cd10533
Location:1001 → 1239

SET_EHMT2; SET domain (including pre-SET and post-SET domains) found in euchromatic histone-lysine N-methyltransferase 2 (EHMT2) and similar proteins

pfam12796
Location:775 → 868

Ank_2; Ankyrin repeats (3 copies)

cd20905
Location:473 → 602

EHMT_ZBD; Zinc-binding domain of euchromatic histone lysine methyltransferases EHMT1 and EHTM2

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086038.1 Reference GRCr8

Range

3924263..3941238 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006255959.4 → XP_006256021.1 histone-lysine N-methyltransferase EHMT2 isoform X9

UniProtKB/TrEMBL

A0A0G2JTA5, A0A8J8XWH8

Conserved Domains (6) summary

PHA03247
Location:23 → 201

PHA03247; large tegument protein UL36; Provisional

COG0666
Location:591 → 783

ANKYR; Ankyrin repeat [Signal transduction mechanisms]

sd00045
Location:747 → 777

ANK; ANK repeat [structural motif]

cd10533
Location:910 → 1148

SET_EHMT2; SET domain (including pre-SET and post-SET domains) found in euchromatic histone-lysine N-methyltransferase 2 (EHMT2) and similar proteins

pfam12796
Location:684 → 777

Ank_2; Ankyrin repeats (3 copies)

cd20905
Location:382 → 511

EHMT_ZBD; Zinc-binding domain of euchromatic histone lysine methyltransferases EHMT1 and EHTM2
XM_063279211.1 → XP_063135281.1 histone-lysine N-methyltransferase EHMT2 isoform X8

UniProtKB/TrEMBL

A0A8J8XWH8
XM_006255958.4 → XP_006256020.1 histone-lysine N-methyltransferase EHMT2 isoform X6

UniProtKB/TrEMBL

A0A0G2JZM5, A0A8J8XWH8

Conserved Domains (6) summary

PHA03247
Location:23 → 201

PHA03247; large tegument protein UL36; Provisional

COG0666
Location:625 → 817

ANKYR; Ankyrin repeat [Signal transduction mechanisms]

sd00045
Location:781 → 811

ANK; ANK repeat [structural motif]

cd10533
Location:944 → 1182

SET_EHMT2; SET domain (including pre-SET and post-SET domains) found in euchromatic histone-lysine N-methyltransferase 2 (EHMT2) and similar proteins

pfam12796
Location:718 → 811

Ank_2; Ankyrin repeats (3 copies)

cd20905
Location:416 → 545

EHMT_ZBD; Zinc-binding domain of euchromatic histone lysine methyltransferases EHMT1 and EHTM2
XM_006255957.4 → XP_006256019.1 histone-lysine N-methyltransferase EHMT2 isoform X5

UniProtKB/TrEMBL

A0A8J8XWH8

Related

ENSRNOP00000071106.2, ENSRNOT00000081456.4

Conserved Domains (5) summary

PHA03247
Location:23 → 201

PHA03247; large tegument protein UL36; Provisional

sd00045
Location:791 → 821

ANK; ANK repeat [structural motif]

cd10533
Location:954 → 1192

SET_EHMT2; SET domain (including pre-SET and post-SET domains) found in euchromatic histone-lysine N-methyltransferase 2 (EHMT2) and similar proteins

pfam12796
Location:728 → 821

Ank_2; Ankyrin repeats (3 copies)

cd20905
Location:416 → 545

EHMT_ZBD; Zinc-binding domain of euchromatic histone lysine methyltransferases EHMT1 and EHTM2
XM_006255956.5 → XP_006256018.3 histone-lysine N-methyltransferase EHMT2 isoform X3

UniProtKB/TrEMBL

A0A8J8XWH8

Related

ENSRNOP00000068632.2, ENSRNOT00000085701.4
XM_006255955.5 → XP_006256017.3 histone-lysine N-methyltransferase EHMT2 isoform X2

UniProtKB/TrEMBL

A0A8J8XWH8
XM_006255954.5 → XP_006256016.3 histone-lysine N-methyltransferase EHMT2 isoform X1

UniProtKB/TrEMBL

A0A8J8XWH8

Related

ENSRNOP00000098065.2, ENSRNOT00000120382.2
XM_039098807.2 → XP_038954735.1 histone-lysine N-methyltransferase EHMT2 isoform X10

Conserved Domains (4) summary

sd00045
Location:516 → 546

ANK; ANK repeat [structural motif]

cd10533
Location:679 → 917

SET_EHMT2; SET domain (including pre-SET and post-SET domains) found in euchromatic histone-lysine N-methyltransferase 2 (EHMT2) and similar proteins

pfam12796
Location:453 → 546

Ank_2; Ankyrin repeats (3 copies)

cd20905
Location:141 → 270

EHMT_ZBD; Zinc-binding domain of euchromatic histone lysine methyltransferases EHMT1 and EHTM2
XM_063279210.1 → XP_063135280.1 histone-lysine N-methyltransferase EHMT2 isoform X7

UniProtKB/TrEMBL

A0A8J8XWH8
XM_063279209.1 → XP_063135279.1 histone-lysine N-methyltransferase EHMT2 isoform X4

UniProtKB/TrEMBL

A0A8J8XWH8