Pck2 phosphoenolpyruvate carboxykinase 2 (mitochondrial) [Rattus norvegicus (Norway rat)] - Gene

Summary

Symbol: Pck2provided by RGD
Full Name: phosphoenolpyruvate carboxykinase 2 (mitochondrial)provided by RGD
Primary source: RGD:1311112
See related: EnsemblRapid:ENSRNOG00000018536 AllianceGenome:RGD:1311112
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: PEPCK-M
Summary: Enables phosphoenolpyruvate carboxykinase (GTP) activity. Involved in several processes, including carboxylic acid metabolic process; gluconeogenesis; and positive regulation of insulin secretion. Predicted to be active in mitochondrial matrix. Orthologous to human PCK2 (phosphoenolpyruvate carboxykinase 2, mitochondrial). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Spleen (RPKM 137.9), Kidney (RPKM 104.9) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 15p13

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	15	NC_086033.1 (32997853..33006691)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	15	NC_051350.1 (29027891..29036729)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	15	NC_005114.4 (34216735..34224357)

Chromosome 15 - NC_086033.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

PEPCK-M has a direct role in fasted and fed glucose homeostasis
Title: A role for mitochondrial phosphoenolpyruvate carboxykinase (PEPCK-M) in the regulation of hepatic gluconeogenesis.
Data show that PEPCK-M message and protein were detected in INS-1 832/13 cells and islets.
Title: Phosphoenolpyruvate cycling via mitochondrial phosphoenolpyruvate carboxykinase links anaplerosis and mitochondrial GTP with insulin secretion.

Submit: New GeneRIF Correction

General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables manganese ion binding	IBA Inferred from Biological aspect of Ancestor more info
enables manganese ion binding	ISO Inferred from Sequence Orthology more info
enables phosphoenolpyruvate carboxykinase (GTP) activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphoenolpyruvate carboxykinase (GTP) activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphoenolpyruvate carboxykinase (GTP) activity	ISO Inferred from Sequence Orthology more info
enables phosphoenolpyruvate carboxykinase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in NADH oxidation	IDA Inferred from Direct Assay more info	PubMed
involved_in NADH oxidation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to dexamethasone stimulus	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to glucose stimulus	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to glucose stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to insulin stimulus	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to tumor necrosis factor	IEP Inferred from Expression Pattern more info	PubMed
involved_in gluconeogenesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in gluconeogenesis	IDA Inferred from Direct Assay more info	PubMed
involved_in gluconeogenesis	IEA Inferred from Electronic Annotation more info
involved_in gluconeogenesis	ISO Inferred from Sequence Orthology more info
involved_in glycerol biosynthetic process from pyruvate	IBA Inferred from Biological aspect of Ancestor more info
involved_in hepatocyte differentiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in oxaloacetate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in oxaloacetate metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of insulin secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in propionate catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in pyruvate metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to dexamethasone	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to lipopolysaccharide	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to starvation	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
is_active_in mitochondrial matrix	IBA Inferred from Biological aspect of Ancestor more info
is_active_in mitochondrial matrix	ISO Inferred from Sequence Orthology more info
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: phosphoenolpyruvate carboxykinase [GTP], mitochondrial

NP_001101847.2

EC 4.1.1.32

EC 4.1.1.49

XP_063130477.1

EC 4.1.1.32

EC 4.1.1.49

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001108377.2 → NP_001101847.2 phosphoenolpyruvate carboxykinase [GTP], mitochondrial

Status: VALIDATED

Source sequence(s)

BC166766, CB787746

UniProtKB/TrEMBL

B2RYG2

Related

ENSRNOP00000025260.7, ENSRNOT00000025260.9

Conserved Domains (1) summary

cd00819
Location:44 → 633

PEPCK_GTP; Phosphoenolpyruvate carboxykinase (PEPCK), a critical gluconeogenic enzyme, catalyzes the first committed step in the diversion of tricarboxylic acid cycle intermediates toward gluconeogenesis. It catalyzes the reversible decarboxylation and ...