Chat choline O-acetyltransferase [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Chatprovided by RGD
Official Full Name: choline O-acetyltransferaseprovided by RGD
Primary source: RGD:1304627
See related: EnsemblRapid:ENSRNOG00000025012 AllianceGenome:RGD:1304627
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables choline O-acetyltransferase activity and choline binding activity. Involved in several processes, including acetylcholine biosynthetic process; antral ovarian follicle growth; and response to ethanol. Predicted to be located in axon and neuronal cell body. Predicted to be active in cytoplasm and neuron projection. Used to study depressive disorder. Biomarker of Alzheimer's disease; asthma; and depressive disorder. Human ortholog(s) of this gene implicated in Alzheimer's disease and congenital myasthenic syndrome 6. Orthologous to human CHAT (choline O-acetyltransferase). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Brain (RPKM 10.4) and Testes (RPKM 4.3) See more
Orthologs: human mouse all
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Genomic context

Location:: 16p16

Exon count:: 17

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	16	NC_086034.1 (7663665..7723416, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	16	NC_051351.1 (7657362..7717093, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	16	NC_005115.4 (8576858..8686131, complement)

Chromosome 16 - NC_086034.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Expression of Cholinergic Markers and Characterization of Splice Variants during Ontogenesis of Rat Dorsal Root Ganglia Neurons.
Title: Expression of Cholinergic Markers and Characterization of Splice Variants during Ontogenesis of Rat Dorsal Root Ganglia Neurons.
Higher choline acetyltransferase activity rostrally than caudally in the trapezoid body is consistent with evidence that the centrifugal cholinergic innervation of the cochlear nucleus derives predominantly from locations at or rostral to its anterior part, in the superior olivary complex and pontomesencephalic tegmentum.
Title: Quantitative distribution of choline acetyltransferase activity in rat trapezoid body.
Data show that choline acetyltransferase (ChAT) immunoreactivity is reduced in the basal forebrain of late adolescent rats (P56) that persists into later adulthood (P220) following exposure to a model of adolescent binge drinking.
Title: Adolescent, but not adult, binge ethanol exposure leads to persistent global reductions of choline acetyltransferase expressing neurons in brain.
Resultus do not support the idea that acetylcholine can be synthesized by an extracellularly located ChAT
Title: No evidence for role of extracellular choline-acetyltransferase in generation of gamma oscillations in rat hippocampal slices in vitro.
Ginsenoside protects against acute renal failure via reduction of renal oxidative stress and enhanced expression of ChAT in the proximal convoluted tubule and ERK1/2 in the paraventricular nuclei.
Title: Protective effect of ginsenoside against acute renal failure via reduction of renal oxidative stress and enhanced expression of ChAT in the proximal convoluted tubule and ERK1/2 in the paraventricular nuclei.
In summary, our present study revealed a remarkable timing and topographic difference on the postnatal development of the nestin expression within the MS-DBB of BF compared with ChAT and PV expression.
Title: Postnatal development of nestin positive neurons in rat basal forebrain: different onset and topography with choline acetyltransferase and parvalbumin expression.
Our results suggest that the differences in the expression level of PKA and ChAT (but not of CaMKIIalpha), as well as the differences in the activation of both PKA and CaMKIIalpha, may contribute to the individual variation in learning skills
Title: Hippocampal levels of ChAT, PKA, phospho-PKA and phospho-CaMKIIα but not CaMKIIα positively correlate with spatial learning skills in rats.
Report differential regulation of ChAT and nNOS in animal models of Crohn's colitis and ulcerative colitis.
Title: Paradoxical regulation of ChAT and nNOS expression in animal models of Crohn's colitis and ulcerative colitis.
Bilateral removal of the flocculi and paraflocculi, however, decreased significantly only AChE, and not choline acetyltransferase, in nodulus or uvula.
Title: Evidence for an intracerebellar acetylcholinesterase-rich but probably non-cholinergic flocculo-nodular projection in the rat.
The presence of pChAT-immunoreactive nerve fibers in rat skin, is reported.
Title: Peripheral choline acetyltransferase in rat skin demonstrated by immunohistochemistry.

Submit: New GeneRIF Correction See all GeneRIFs (33)

General gene information

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Markers

RH138865 (e-PCR)
- UniSTS:220952

NoName (e-PCR)
- UniSTS:547010

Slc18a3 (e-PCR), detects polymorphism
- UniSTS:547010

Chat (e-PCR), detects polymorphism
- UniSTS:265455

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables choline O-acetyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables choline O-acetyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables choline O-acetyltransferase activity	ISO Inferred from Sequence Orthology more info
enables choline binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in acetylcholine biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in acetylcholine biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within adult walking behavior	ISO Inferred from Sequence Orthology more info
involved_in antral ovarian follicle growth	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within chemical synaptic transmission	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within dendrite development	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within establishment of synaptic specificity at neuromuscular junction	ISO Inferred from Sequence Orthology more info
involved_in memory	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within muscle organ development	ISO Inferred from Sequence Orthology more info
involved_in neuromuscular synaptic transmission	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within neuromuscular synaptic transmission	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within neuron differentiation	ISO Inferred from Sequence Orthology more info
involved_in response to ethanol	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to hypoxia	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to nutrient	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to xenobiotic stimulus	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within rhythmic behavior	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within rhythmic excitation	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in axon	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in neuron projection	IBA Inferred from Biological aspect of Ancestor more info
located_in neuron projection	ISO Inferred from Sequence Orthology more info
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info
located_in synapse	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: choline O-acetyltransferase

Names: CHOACTase; choline acetylase; choline acetyltransferase

NP_001164064.1

EC 2.3.1.6

XP_063131208.1

EC 2.3.1.6

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.