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    Slc28a2 solute carrier family 28 member 2 [ Rattus norvegicus (Norway rat) ]

    Gene ID: 60423, updated on 9-Dec-2024

    Summary

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    Symbol
    Slc28a2provided by RGD
    Full Name
    solute carrier family 28 member 2provided by RGD
    Primary source
    RGD:61840
    See related
    EnsemblRapid:ENSRNOG00000028668 AllianceGenome:RGD:61840
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Rattus norvegicus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
    Summary
    Enables nucleoside:sodium symporter activity. Involved in protein localization to cell surface and retina homeostasis. Located in coated vesicle; plasma membrane; and vesicle membrane. Orthologous to human SLC28A2 (solute carrier family 28 member 2). [provided by Alliance of Genome Resources, Dec 2024]
    Expression
    Biased expression in Liver (RPKM 708.8), Heart (RPKM 216.1) and 8 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Slc28a2 in Genome Data Viewer
    Location:
    3q35
    Exon count:
    22
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCr8 (GCF_036323735.1) 3 NC_086021.1 (129778425..129841292)
    RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 3 NC_051338.1 (109324815..109387702)
    106 previous assembly Rnor_6.0 (GCF_000001895.5) 3 NC_005102.4 (114355003..114647382)

    Chromosome 3 - NC_086021.1Genomic Context describing neighboring genes Neighboring gene transfer RNA histidine (anticodon GUG) 7 Neighboring gene transfer RNA histidine (anticodon GUG) 8 Neighboring gene transfer RNA histidine (anticodon GUG) 9 Neighboring gene U4 spliceosomal RNA Neighboring gene sodium/nucleoside cotransporter 2-like

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Characterization of the rat Na+/nucleoside cotransporter 2 and transport of nucleoside-derived drugs using electrophysiological methods. Larráyoz IM, et al. Am J Physiol Cell Physiol, 2006 Dec. PMID 16837649
    2. A nucleoside transporter is functionally linked to ectonucleotidases in rat liver canalicular membrane. Che M, et al. J Biol Chem, 1992 May 15. PMID 1315767
    3. Transport of adenosine by recombinant purine- and pyrimidine-selective sodium/nucleoside cotransporters from rat jejunum expressed in Xenopus laevis oocytes. Yao SY, et al. Mol Pharmacol, 1996 Dec. PMID 8967974
    4. Demonstration of the existence of mRNAs encoding N1/cif and N2/cit sodium/nucleoside cotransporters in rat brain. Anderson CM, et al. Brain Res Mol Brain Res, 1996 Dec. PMID 9013795
    5. Cloned blood-brain barrier adenosine transporter is identical to the rat concentrative Na+ nucleoside cotransporter CNT2. Li JY, et al. J Cereb Blood Flow Metab, 2001 Aug. PMID 11487728

    See all (23) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The purpose of this study is to determine the localization of nucleoside transporters expressed within rat epididymis to demonstrate the potential of epididymal reabsorption. Using immunohistochemistry, we determined that equilibrative nucleoside transporter 1 (ENT1) is localized to the basolateral membrane of epithelial cells, ENT2 is expressed in the nucleus of the epithelium and CNT2 is expressed by basal cells.
      Title: Localization of nucleoside transporters in rat epididymis.
    2. rCNT2 extracellular cysteines, Cys(615) and Cys(649), are important for maturation and sorting to the plasma membrane
      Title: rCNT2 extracellular cysteines, Cys(615) and Cys(649), are important for maturation and sorting to the plasma membrane.
    3. CNT2 is likely to modulate extracellular adenosine and cell energy balance in neuronal tissue.
      Title: Hypoxia and P1 receptor activation regulate the high-affinity concentrative adenosine transporter CNT2 in differentiated neuronal PC12 cells.
    4. Report induction of CNT2 in experimental diabetes mellitus.
      Title: Mechanism of nucleoside uptake in rat placenta and induction of placental CNT2 in experimental diabetes.
    5. the N-terminal tail of rCNT2 contains dual sorting signals. An acidic region is responsible for its proper stabilization at the plasma membrane, whereas the putative CK2 domain (Ser(46)) is implicated in the apical sorting of the transporter.
      Title: Structural determinants for rCNT2 sorting to the plasma membrane of polarized and non-polarized cells.
    6. Data concluded that CNT2 contributes considerably to nucleoside uptake in rat hepatocytes but not in mouse hepatocytes.
      Title: Striking species difference in the contribution of concentrative nucleoside transporter 2 to nucleoside uptake between mouse and rat hepatocytes.
    7. The relative distribution of SLC28 gene expression suggests a role for the proximal tubule in renal nucleoside clearance and an accessory role for CNT2 and CNT3, in adenosine-mediated regulation of collecting duct functions.
      Title: Expression of concentrative nucleoside transporters SLC28 (CNT1, CNT2, and CNT3) along the rat nephron: effect of diabetes.
    8. a new physiological role for the transporter in the modulation of extracellular adenosine levels and the sleep/wakefulness cycle
      Title: Distribution of CNT2 and ENT1 transcripts in rat brain: selective decrease of CNT2 mRNA in the cerebral cortex of sleep-deprived rats.
    9. Results identify the critical domains and amino acid residues that contribute to the observed difference in specificity between concentrative nucleoside transporter (CNT)2 orthologs.
      Title: Molecular determinants of specificity for synthetic nucleoside analogs in the concentrative nucleoside transporter, CNT2.
    10. Bile acids were found to increase CNT2-related transport activity in a K(ATP) channel-independent manner in both Fao hepatoma and rat liver parenchymal cells.
      Title: Bile acids alter the subcellular localization of CNT2 (concentrative nucleoside cotransporter) and increase CNT2-related transport activity in liver parenchymal cells.
    Submit: New GeneRIF Correction See all GeneRIFs (13)

    Pathways from PubChem

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    General gene information

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    Markers

    Homology

    Gene Ontology Provided by RGD

    Function Evidence Code Pubs
    enables neurotransmitter transmembrane transporter activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables nucleoside transmembrane transporter activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables nucleoside transmembrane transporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleoside:sodium symporter activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables nucleoside:sodium symporter activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables nucleoside:sodium symporter activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables purine nucleobase transmembrane transporter activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables purine nucleoside transmembrane transporter activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables purine nucleoside transmembrane transporter activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables pyrimidine- and adenosine-specific:sodium symporter activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables uridine transmembrane transporter activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    involved_in adenosine transport ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in inosine transport ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in neurotransmitter transport ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in nucleoside transmembrane transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in nucleoside transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in nucleoside transmembrane transport ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein localization to cell surface IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in purine nucleobase transmembrane transport ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in purine nucleoside transmembrane transport ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in pyrimidine nucleobase transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in pyrimidine-containing compound transmembrane transport ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in retina homeostasis IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in retina homeostasis IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in uridine transmembrane transport ISO
    Inferred from Sequence Orthology
    more info
    		  
    Component Evidence Code Pubs
    located_in apicolateral plasma membrane ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in apicolateral plasma membrane ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    located_in brush border membrane ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in coated vesicle IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in vesicle membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    sodium/nucleoside cotransporter 2
    Names
    CNT 2
    Na(+)/nucleoside cotransporter 2
    SPNT
    concentrative nucleoside transporter 2
    rCNT2
    sodium-coupled nucleoside transporter 2
    sodium/nucleoside cotransporter 2-like
    sodium/purine nucleoside cotransporter
    solute carrier family 28 (concentrative nucleoside transporter), member 2
    solute carrier family 28 (sodium-coupled nucleoside transporter), member 2

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_031664.3 → NP_113852.2  sodium/nucleoside cotransporter 2

      Status: VALIDATED

      Source sequence(s)
      JAXUCZ010000003
      UniProtKB/TrEMBL
      F1LN73, Q91WW8
      Related
      ENSRNOP00000024658.7, ENSRNOT00000024658.9
      Conserved Domains (1) summary
      TIGR00804
      Location:175 → 586
      nupC; nucleoside transporter

    RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCr8

    Genomic

    1. NC_086021.1 Reference GRCr8

      Range
      129778425..129841292
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_063284461.1 → XP_063140531.1  sodium/nucleoside cotransporter 2 isoform X1

      UniProtKB/TrEMBL
      F1LN73, Q91WW8

    2. XM_063284459.1 → XP_063140529.1  sodium/nucleoside cotransporter 2 isoform X1

      UniProtKB/TrEMBL
      F1LN73, Q91WW8

    3. XM_063284462.1 → XP_063140532.1  sodium/nucleoside cotransporter 2 isoform X1

      UniProtKB/TrEMBL
      F1LN73, Q91WW8

    4. XM_039105769.2 → XP_038961697.1  sodium/nucleoside cotransporter 2 isoform X1

      UniProtKB/TrEMBL
      F1LN73, Q91WW8
      Conserved Domains (1) summary
      TIGR00804
      Location:175 → 586
      nupC; nucleoside transporter

    5. XM_063284460.1 → XP_063140530.1  sodium/nucleoside cotransporter 2 isoform X1

      UniProtKB/TrEMBL
      F1LN73, Q91WW8

    6. XM_039105768.2 → XP_038961696.1  sodium/nucleoside cotransporter 2 isoform X1

      UniProtKB/TrEMBL
      F1LN73, Q91WW8
      Conserved Domains (1) summary
      TIGR00804
      Location:175 → 586
      nupC; nucleoside transporter

    7. XM_039105770.2 → XP_038961698.1  sodium/nucleoside cotransporter 2 isoform X1

      UniProtKB/TrEMBL
      F1LN73, Q91WW8
      Conserved Domains (1) summary
      TIGR00804
      Location:175 → 586
      nupC; nucleoside transporter

    RNA

    1. XR_010064689.1 RNA Sequence

    2. XR_005501974.2 RNA Sequence

    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q62773.1 GenPept UniProtKB/Swiss-Prot:Q62773

    Gene LinkOut

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