Hsd3b5 hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 5 [Rattus norvegicus (Norway rat)] - Gene

Summary

Symbol: Hsd3b5provided by RGD
Full Name: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 5provided by RGD
Primary source: RGD:2838
See related: EnsemblRapid:ENSRNOG00000019417 AllianceGenome:RGD:2838
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Hsd1; Hsd3b; RATHSDI
Summary: Enables 3-beta-hydroxy-delta5-steroid dehydrogenase (NAD+) activity and steroid binding activity. Involved in progesterone metabolic process; regulation of steroid biosynthetic process; and steroid biosynthetic process. Predicted to be located in endoplasmic reticulum membrane and mitochondrial membrane. Predicted to be active in cytoplasm and intracellular membrane-bounded organelle. Human ortholog(s) of this gene implicated in hypertension and hypospadias. Orthologous to human HSD3B1 (hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1) and HSD3B2 (hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Adrenal (RPKM 1941.1), Liver (RPKM 392.8) and 1 other tissue See more
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Genomic context

Location:: 2q34

Exon count:: 4

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	2	NC_086020.1 (188697669..188717141, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	2	NC_051337.1 (186008944..186028417, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	2	NC_005101.4 (200615910..200633317, complement)

Chromosome 2 - NC_086020.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

these steroidogenic enzymes within the Leydig cells, including steroidogenic acute regulatory protein (StAR), cholesterol side-chain cleavage cytochrome P450 (P450scc) and 3beta-hydroxysteroid dehydrogenase (3beta-HSD), were down-regulated.
Title: HC diet inhibited testosterone synthesis by activating endoplasmic reticulum stress in testicular Leydig cells.
ERalpha interaction with transcription coregulators, histone acetylases (CBP/SRC-1), and PGC-1alpha is crucial to FSH and TGFbeta1-up-regulated expression of Hsd3b and Cyp11a1, and, thus, progesterone production in rat ovarian granulosa cells.
Title: Crucial role of estrogen receptor-alpha interaction with transcription coregulators in follicle-stimulating hormone and transforming growth factor beta1 up-regulation of steroidogenesis in rat ovarian granulosa cells.
Significant decrease in the 3beta-HSD mRNA expression in traumatic brain injury site within the frontal cortex in both males and pseudopregnant females.
Title: 3beta-Hydroxysteroid dehydrogenase/5-ene-4-ene isomerase mRNA expression in rat brain: effect of pseudopregnancy and traumatic brain injury.
In ovariectomized-adrenalectomized rats, estrogen treatment increased 3beta-HSD mRNA in the hypothalamus.
Title: Neurosteroids and female reproduction: estrogen increases 3beta-HSD mRNA and activity in rat hypothalamus.
Expression of Hsd3b throughout the spinal cord even after castration and adrenalectomy suggests a potential endogenous production of progesterone and an important signalling function of this steroid in the spinal cord.
Title: 3 Beta-hydroxysteroid dehydrogenase expression in rat spinal cord.

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General gene information

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Markers

RH132844 (e-PCR)
- UniSTS:216127

D2Wox25 (e-PCR)
- UniSTS:118131

Hsd3b2 (e-PCR), detects polymorphism
- UniSTS:256372

d2arb20 (e-PCR)
- UniSTS:121582

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables 3-beta-hydroxy-delta5-steroid dehydrogenase (NAD+) activity	IDA Inferred from Direct Assay more info	PubMed
enables 3-keto sterol reductase activity	IEA Inferred from Electronic Annotation more info
enables 5alpha-androstane-3beta,17beta-diol dehydrogenase activity	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor	IBA Inferred from Biological aspect of Ancestor more info
enables oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor	IEA Inferred from Electronic Annotation more info
enables steroid binding	IDA Inferred from Direct Assay more info	PubMed
enables steroid dehydrogenase activity	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
involved_in C21-steroid hormone metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in progesterone metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of steroid biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in steroid biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in steroid biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in steroid biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in steroid biosynthetic process	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
is_active_in intracellular membrane-bounded organelle	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrial membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: NADPH-dependent 3-keto-steroid reductase Hsd3b5

Names: 3 beta-HSD III; 3 beta-hydroxysteroid dehydrogenase 5; 3 beta-hydroxysteroid dehydrogenase type 3; 3 beta-hydroxysteroid dehydrogenase type 5; 3 beta-hydroxysteroid dehydrogenase type 5-like; 3 beta-hydroxysteroid dehydrogenase type III; 3 beta-hydroxysteroid dehydrogenase type V; 3-beta-HSD V; 3-beta-hydroxy-5-ene steroid dehydrogenase; NADPH-dependent 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; dihydrotestosterone 3-ketoreductase; progesterone reductase; steroid delta-isomerase, 3 beta

NP_036716.2

EC 1.1.1.210

EC 1.1.1.270

XP_038957645.1

EC 1.1.1.210

EC 1.1.1.270

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_012584.2 → NP_036716.2 NADPH-dependent 3-keto-steroid reductase Hsd3b5

Status: PROVISIONAL

Source sequence(s)

JAXUCZ010000002

UniProtKB/Swiss-Prot

P27364, Q569C6

UniProtKB/TrEMBL

A0A0G2JSR3, A6K3D9

Related

ENSRNOP00000072555.1, ENSRNOT00000079754.3

Conserved Domains (1) summary

cl21454
Location:5 → 357

NADB_Rossmann; Rossmann-fold NAD(P)(+)-binding proteins

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086020.1 Reference GRCr8

Range

188697669..188717141 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039101717.2 → XP_038957645.1 NADPH-dependent 3-keto-steroid reductase Hsd3b5 isoform X1

UniProtKB/Swiss-Prot

P27364, Q569C6

Conserved Domains (1) summary

cl21454
Location:5 → 302

NADB_Rossmann; Rossmann-fold NAD(P)(+)-binding proteins