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    Ldhb lactate dehydrogenase B [ Rattus norvegicus (Norway rat) ]

    Gene ID: 24534, updated on 9-Dec-2024

    Summary

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    Official Symbol
    Ldhbprovided by RGD
    Official Full Name
    lactate dehydrogenase Bprovided by RGD
    Primary source
    RGD:2997
    See related
    EnsemblRapid:ENSRNOG00000013000 AllianceGenome:RGD:2997
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Rattus norvegicus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
    Also known as
    Ldh2
    Summary
    This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have also been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 9. [provided by RefSeq, Feb 2016]
    Expression
    Biased expression in Heart (RPKM 12336.6), Kidney (RPKM 2578.0) and 3 other tissues See more
    Orthologs
    human mouse all
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    Genomic context

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    See Ldhb in Genome Data Viewer
    Location:
    4q44
    Exon count:
    9
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCr8 (GCF_036323735.1) 4 NC_086022.1 (177159389..177177408, complement)
    RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 4 NC_051339.1 (175428382..175446403, complement)
    106 previous assembly Rnor_6.0 (GCF_000001895.5) 4 NC_005103.4 (176701980..176719999, complement)

    Chromosome 4 - NC_086022.1Genomic Context describing neighboring genes Neighboring gene spexin hormone Neighboring gene glycogen synthase 2 Neighboring gene potassium inwardly-rectifying channel, subfamily J, member 8 Neighboring gene ATP binding cassette subfamily C member 9

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
    • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
    • BioProject: PRJNA238328
    • Publication: PMID 24510058
    • Analysis date: Mon Jun 6 17:44:12 2016

    Bibliography

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    Related articles in PubMed

    1. MicroRNA-375-3p inhibitor suppresses angiotensin II-induced cardiomyocyte hypertrophy by promoting lactate dehydrogenase B expression. Feng H, et al. J Cell Physiol, 2019 Aug. PMID 30618075
    2. Purification and radioimmunoassay of rat lactate dehydrogenase A and B subunits. Beebee TJ, et al. Biochem J, 1982 Aug 1. PMID 7138505, Free PMC Article
    3. Lactate dehydrogenase regulation in aged skeletal muscle: Regulation by anabolic steroids and functional overload. Washington TA, et al. Exp Gerontol, 2014 Sep. PMID 24835193
    4. Circadian rhythm of metabolic oscillation in suprachiasmatic nucleus depends on the mitochondrial oxidation state, reflected by cytochrome C oxidase and lactate dehydrogenase. Isobe Y, et al. J Neurosci Res, 2011 Jun. PMID 21416482
    5. Mammalian oocytes: X chromosome activity. Epstein CJ. Science, 1969 Mar 7. PMID 5764873

    See all (36) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. miR-375-3p inhibitor suppresses Ang II-induced cardiomyocyte hypertrophy by promoting LDHB expression.
      Title: MicroRNA-375-3p inhibitor suppresses angiotensin II-induced cardiomyocyte hypertrophy by promoting lactate dehydrogenase B expression.
    2. The readthrough-extended lactate dehydrogenase subunit LDHBx can also co-import LDHA, the other LDH subunit, into peroxisomes.
      Title: Peroxisomal lactate dehydrogenase is generated by translational readthrough in mammals.
    3. aging alters aspects of skeletal muscle isoform shift induced by overload and anabolic steroid administration
      Title: Lactate dehydrogenase regulation in aged skeletal muscle: Regulation by anabolic steroids and functional overload.
    4. LDH and its coding mRNA were higher during the dark period.
      Title: Circadian rhythm of metabolic oscillation in suprachiasmatic nucleus depends on the mitochondrial oxidation state, reflected by cytochrome C oxidase and lactate dehydrogenase.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by RGD

    Function Evidence Code Pubs
    enables L-lactate dehydrogenase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables L-lactate dehydrogenase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables L-lactate dehydrogenase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables NAD binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables identical protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables identical protein binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables kinase binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables lactate dehydrogenase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in NAD metabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in lactate metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in lactate metabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in lactate metabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in pyruvate metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in pyruvate metabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytosol ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in cytosol ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in membrane raft ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in mitochondrial inner membrane ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in mitochondrial inner membrane ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    is_active_in mitochondrion IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of oxidoreductase complex ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    L-lactate dehydrogenase B chain
    Names
    LDH heart subunit
    LDH-B
    LDH-H
    Lactate dehydrogenease B
    NP_001303262.1
    NP_001303263.1
    NP_036727.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001316333.1 → NP_001303262.1  L-lactate dehydrogenase B chain isoform Ldhbx

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (Ldhb) results from translation termination at the upstream UGA stop codon, while the longer isoform (Ldhbx) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the longer, C-terminally extended isoform (Ldhbx), which is localized in the peroxisomes. As the UGA stop codon has been reported to specify several alternative amino acids (tryptophan, cysteine, arginine and serine), its location in the longer isoform is denoted by an 'X'.
      Source sequence(s)
      BU759234, FM054345, U07181
      UniProtKB/Swiss-Prot
      P42123
      Conserved Domains (2) summary
      cd05293
      Location:20 → 330
      LDH_1; A subgroup of L-lactate dehydrogenases
      PLN02602
      Location:4 → 332
      PLN02602; lactate dehydrogenase

    2. NM_001316334.1 → NP_001303263.1  L-lactate dehydrogenase B chain isoform Ldhb

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (Ldhb).
      Source sequence(s)
      BU759234, CK355620, FM054345, U07181
      UniProtKB/Swiss-Prot
      P42123
      UniProtKB/TrEMBL
      A6IMU4
      Related
      ENSRNOP00000017965.4, ENSRNOT00000017965.9
      Conserved Domains (1) summary
      cd05293
      Location:20 → 330
      LDH_1; A subgroup of L-lactate dehydrogenases

    3. NM_012595.2 → NP_036727.1  L-lactate dehydrogenase B chain isoform Ldhb

      See identical proteins and their annotated locations for NP_036727.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (Ldhb) results from translation termination at the upstream UGA stop codon, while the longer isoform (Ldhbx) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the shorter isoform (Ldhb), which is localized in the cytosol. Variants 1 and 2 encode the same isoform.
      Source sequence(s)
      BU759234, FM054345, U07181
      UniProtKB/Swiss-Prot
      P42123
      UniProtKB/TrEMBL
      A6IMU4
      Conserved Domains (1) summary
      cd05293
      Location:20 → 330
      LDH_1; A subgroup of L-lactate dehydrogenases

    RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCr8

    Genomic

    1. NC_086022.1 Reference GRCr8

      Range
      177159389..177177408 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P42123.2 GenPept UniProtKB/Swiss-Prot:P42123

    Gene LinkOut

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