Smad5 SMAD family member 5 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Smad5provided by RGD
Official Full Name: SMAD family member 5provided by RGD
Primary source: RGD:620158
See related: EnsemblRapid:ENSRNOG00000022870 AllianceGenome:RGD:620158
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Madh5
Summary: Predicted to enable several functions, including DNA-binding transcription repressor activity, RNA polymerase II-specific; I-SMAD binding activity; and enzyme binding activity. Involved in Mullerian duct regression and ossification. Predicted to be located in cytosol and nucleoplasm. Predicted to be part of heteromeric SMAD protein complex. Orthologous to human SMAD5 (SMAD family member 5). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Adrenal (RPKM 145.0), Lung (RPKM 139.4) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 17p14

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	17	NC_086035.1 (7867640..7896983, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	17	NC_051352.1 (7862332..7891678, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	17	NC_005116.4 (8307448..8336996, complement)

Chromosome 17 - NC_086035.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

MiR-122-5p promotes peritoneal fibrosis in a rat model of peritoneal dialysis by targeting Smad5 to activate Wnt/beta-catenin pathway.
Title: MiR-122-5p promotes peritoneal fibrosis in a rat model of peritoneal dialysis by targeting Smad5 to activate Wnt/β-catenin pathway.
The activation and regulation of STX12, miR-148a and SMAD5 in pancreatic stellate cells may serve as a new gene therapy strategy for the treatment of chronic pancreatitis and reversal of pancreatic fibrosis.
Title: STX12 lncRNA/miR-148a/SMAD5 participate in the regulation of pancreatic stellate cell activation through a mechanism involving competing endogenous RNA.
Cpd 861 markedly upregulated the gene expression of BMP7 and Smad5, as well as the protein expression of BMP7 and Smad1/5. In addition, p-Smad1/5/8 protein expression was markedly increased by Cpd 861 in the liver fibrosis model.
Title: Attenuation of liver fibrosis by herbal compound 861 via upregulation of BMP-7/Smad signaling in the bile duct ligation model rat.
Taken together, these results indicated that FSTL1 protects the SNP-induced injury in cardiac H9c2 cells through, at least in part, the activation of Akt and inhibition of Smad1/5/9 signaling.
Title: Follistatin-like 1 protects cardiomyoblasts from injury induced by sodium nitroprusside through modulating Akt and Smad1/5/9 signaling.
the BMP-2-SMAD-5 pathway has a role in hydroxyapatite differentiation of aortic smooth muscle cells into osteoblast-like cells
Title: Rat aortic smooth muscle cells cultured on hydroxyapatite differentiate into osteoblast-like cells via BMP-2-SMAD-5 pathway.
SMAD1, 5, and 8 transcripts are localized to trigeminal ganglia axons and these mRNAs are selectively translated in response to BDNF.
Title: Intra-axonal translation of SMAD1/5/8 mediates retrograde regulation of trigeminal ganglia subtype specification.
biomechanical stimulation of osteoblasts activates SMAD 1/5/8 in the BMP signaling pathway through BMPR1 and may enhance osteogenesis by upregulating SMAD-dependent osteogenic genes
Title: Biomechanical forces exert anabolic effects on osteoblasts by activation of SMAD 1/5/8 through type 1 BMP receptor.
Prostacyclin analogues inhibited smooth muscle cell proliferation and prevented progression of pulmonary hypertension while enhancing Smad1/5 phosphorylation and Id1 gene expression.
Title: Smad-dependent and smad-independent induction of id1 by prostacyclin analogues inhibits proliferation of pulmonary artery smooth muscle cells in vitro and in vivo.
The present study indicates that Smad5 regulates glucose uptake in skeletal muscle by controlling Akt2 expression and phosphorylation.
Title: Smad5 regulates Akt2 expression and insulin-induced glucose uptake in L6 myotubes.
Expression of BMP receptors Ib and II, and Smad 5 was downregulated in lungs but not kidneys of MCT-treated rats.
Title: Dysregulated bone morphogenetic protein signaling in monocrotaline-induced pulmonary arterial hypertension.

Submit: New GeneRIF Correction

General gene information

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Markers

RH136684 (e-PCR)
- UniSTS:218773

RH94764 (e-PCR)
- UniSTS:120011

Smad5 (e-PCR), detects polymorphism
- UniSTS:478946

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables DEAD/H-box RNA helicase binding	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription repressor activity, RNA polymerase II-specific	ISO Inferred from Sequence Orthology more info
enables I-SMAD binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables sequence-specific double-stranded DNA binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in BMP signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within BMP signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in BMP signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in BMP signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in Mullerian duct regression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in SMAD protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in SMAD protein signal transduction	ISO Inferred from Sequence Orthology more info
involved_in anatomical structure morphogenesis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within bone development	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cardiac muscle contraction	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cartilage development	ISO Inferred from Sequence Orthology more info
involved_in cell differentiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in cell differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cellular response to organic cyclic compound	ISO Inferred from Sequence Orthology more info
involved_in embryonic pattern specification	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within erythrocyte differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within germ cell development	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of Fas signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of negative regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in ossification	IEP Inferred from Expression Pattern more info	PubMed
involved_in osteoblast differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within osteoblast fate commitment	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of osteoblast differentiation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of osteoblast differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in regulation of ossification	NAS Non-traceable Author Statement more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in transforming growth factor beta receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within transforming growth factor beta receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in ureteric bud development	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
part_of heteromeric SMAD protein complex	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info
part_of transcription regulator complex	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: mothers against decapentaplegic homolog 5

Names: MAD homolog 5; SMAD 5; SMAD, mothers against DPP homolog 5; mothers against DPP homolog 5

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_021692.1 → NP_067724.1 mothers against decapentaplegic homolog 5

See identical proteins and their annotated locations for NP_067724.1

Status: PROVISIONAL

Source sequence(s)

AB010955

UniProtKB/Swiss-Prot

Q9R1V3

UniProtKB/TrEMBL

A0A8I5Y0N4, B1WBR0

Related

ENSRNOP00000016704.3, ENSRNOT00000016704.6

Conserved Domains (2) summary

cd10490
Location:10 → 133

MH1_SMAD_1_5_9; N-terminal Mad Homology 1 (MH1) domain in SMAD1, SMAD5 and SMAD9 (also known as SMAD8)

cd10497
Location:265 → 465

MH2_SMAD_1_5_9; C-terminal Mad Homology 2 (MH2) domain in SMAD1, SMAD5 and SMAD9

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086035.1 Reference GRCr8

Range

7867640..7896983 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006253587.5 → XP_006253649.1 mothers against decapentaplegic homolog 5 isoform X1

See identical proteins and their annotated locations for XP_006253649.1

UniProtKB/Swiss-Prot

Q9R1V3

UniProtKB/TrEMBL

A0A8I5Y0N4, B1WBR0

Conserved Domains (2) summary

cd10490
Location:10 → 133

MH1_SMAD_1_5_9; N-terminal Mad Homology 1 (MH1) domain in SMAD1, SMAD5 and SMAD9 (also known as SMAD8)

cd10497
Location:265 → 465

MH2_SMAD_1_5_9; C-terminal Mad Homology 2 (MH2) domain in SMAD1, SMAD5 and SMAD9
XM_006253586.5 → XP_006253648.1 mothers against decapentaplegic homolog 5 isoform X1

See identical proteins and their annotated locations for XP_006253648.1

UniProtKB/Swiss-Prot

Q9R1V3

UniProtKB/TrEMBL

A0A8I5Y0N4, B1WBR0

Conserved Domains (2) summary

cd10490
Location:10 → 133

MH1_SMAD_1_5_9; N-terminal Mad Homology 1 (MH1) domain in SMAD1, SMAD5 and SMAD9 (also known as SMAD8)

cd10497
Location:265 → 465

MH2_SMAD_1_5_9; C-terminal Mad Homology 2 (MH2) domain in SMAD1, SMAD5 and SMAD9
XM_039096036.2 → XP_038951964.1 mothers against decapentaplegic homolog 5 isoform X1

UniProtKB/Swiss-Prot

Q9R1V3

UniProtKB/TrEMBL

A0A8I5Y0N4, B1WBR0

Conserved Domains (2) summary

cd10490
Location:10 → 133

MH1_SMAD_1_5_9; N-terminal Mad Homology 1 (MH1) domain in SMAD1, SMAD5 and SMAD9 (also known as SMAD8)

cd10497
Location:265 → 465

MH2_SMAD_1_5_9; C-terminal Mad Homology 2 (MH2) domain in SMAD1, SMAD5 and SMAD9
XM_063276717.1 → XP_063132787.1 mothers against decapentaplegic homolog 5 isoform X1

UniProtKB/Swiss-Prot

Q9R1V3

UniProtKB/TrEMBL

A0A8I5Y0N4, B1WBR0
XM_017600658.3 → XP_017456147.1 mothers against decapentaplegic homolog 5 isoform X1

UniProtKB/Swiss-Prot

Q9R1V3

UniProtKB/TrEMBL

A0A8I5Y0N4, B1WBR0

Conserved Domains (2) summary

cd10490
Location:10 → 133

MH1_SMAD_1_5_9; N-terminal Mad Homology 1 (MH1) domain in SMAD1, SMAD5 and SMAD9 (also known as SMAD8)

cd10497
Location:265 → 465

MH2_SMAD_1_5_9; C-terminal Mad Homology 2 (MH2) domain in SMAD1, SMAD5 and SMAD9
XM_006253588.5 → XP_006253650.1 mothers against decapentaplegic homolog 5 isoform X1

See identical proteins and their annotated locations for XP_006253650.1

UniProtKB/Swiss-Prot

Q9R1V3

UniProtKB/TrEMBL

A0A8I5Y0N4, B1WBR0

Conserved Domains (2) summary

cd10490
Location:10 → 133

MH1_SMAD_1_5_9; N-terminal Mad Homology 1 (MH1) domain in SMAD1, SMAD5 and SMAD9 (also known as SMAD8)

cd10497
Location:265 → 465

MH2_SMAD_1_5_9; C-terminal Mad Homology 2 (MH2) domain in SMAD1, SMAD5 and SMAD9
XM_039096035.2 → XP_038951963.1 mothers against decapentaplegic homolog 5 isoform X1

UniProtKB/Swiss-Prot

Q9R1V3

UniProtKB/TrEMBL

A0A8I5Y0N4, B1WBR0

Conserved Domains (2) summary

cd10490
Location:10 → 133

MH1_SMAD_1_5_9; N-terminal Mad Homology 1 (MH1) domain in SMAD1, SMAD5 and SMAD9 (also known as SMAD8)

cd10497
Location:265 → 465

MH2_SMAD_1_5_9; C-terminal Mad Homology 2 (MH2) domain in SMAD1, SMAD5 and SMAD9