Med25 mediator complex subunit 25 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Med25provided by RGD
Official Full Name: mediator complex subunit 25provided by RGD
Primary source: RGD:1305137
See related: EnsemblRapid:ENSRNOG00000020363 AllianceGenome:RGD:1305137
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: RGD1305137
Summary: Predicted to enable nuclear retinoid X receptor binding activity and transcription coactivator binding activity. Predicted to be involved in negative regulation of fibroblast proliferation; positive regulation of mediator complex assembly; and regulation of transcription by RNA polymerase II. Predicted to be located in nucleoplasm. Predicted to be part of core mediator complex and mediator complex. Orthologous to human MED25 (mediator complex subunit 25). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Kidney (RPKM 63.2), Adrenal (RPKM 55.1) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 1q22

Exon count:: 20

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	1	NC_086019.1 (104496447..104513061, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	1	NC_051336.1 (95359961..95375827, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	1	NC_005100.4 (100872240..100887864, complement)

Chromosome 1 - NC_086019.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data show that Med25 is coordinately expressed with Pmp22 gene dosage and expression in transgenic mice and rats, and suggest a potential role of this protein in the molecular etiology of Charcot-Marie-Tooth disease.
Title: Identification of the variant Ala335Val of MED25 as responsible for CMT2B2: molecular data, functional studies of the SH3 recognition motif and correlation between wild-type MED25 and PMP22 RNA levels in CMT1A animal models.

Submit: New GeneRIF Correction

General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables nuclear retinoic acid receptor binding	ISO Inferred from Sequence Orthology more info
enables nuclear retinoid X receptor binding	ISO Inferred from Sequence Orthology more info
enables transcription coactivator binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in biological_process	ND No biological Data available more info
involved_in negative regulation of fibroblast proliferation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of mediator complex assembly	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
acts_upstream_of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
part_of core mediator complex	ISO Inferred from Sequence Orthology more info
part_of mediator complex	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
part_of transcription regulator complex	IBA Inferred from Biological aspect of Ancestor more info
part_of transcription regulator complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: mediator of RNA polymerase II transcription subunit 25

Names: mediator of RNA polymerase II transcription, subunit 25 homolog

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001170426.2 → NP_001163897.1 mediator of RNA polymerase II transcription subunit 25 isoform 3

Status: VALIDATED

Source sequence(s)

JAXUCZ010000001

UniProtKB/TrEMBL

A6JAU0

Related

ENSRNOP00000094991.1, ENSRNOT00000117952.2

Conserved Domains (4) summary

pfam11232
Location:401 → 546

Med25; Mediator complex subunit 25 PTOV activation and synapsin 2

pfam11235
Location:228 → 384

Med25_SD1; Mediator complex subunit 25 synapsin 1

pfam11244
Location:664 → 746

Med25_NR-box; Mediator complex subunit 25 C-terminal NR box-containing

pfam11265
Location:13 → 225

Med25_VWA; Mediator complex subunit 25 von Willebrand factor type A
NM_001415793.1 → NP_001402722.1 mediator of RNA polymerase II transcription subunit 25 isoform 1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000001

UniProtKB/TrEMBL

A6JAU0
NM_001415794.1 → NP_001402723.1 mediator of RNA polymerase II transcription subunit 25 isoform 2

Status: VALIDATED

Source sequence(s)

JAXUCZ010000001

UniProtKB/TrEMBL

A6JAU0

Related

ENSRNOP00000027675.7, ENSRNOT00000027675.8

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086019.1 Reference GRCr8

Range

104496447..104513061 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063284057.1 → XP_063140127.1 mediator of RNA polymerase II transcription subunit 25 isoform X2

UniProtKB/TrEMBL

A0A8I6B1T5
XM_006229010.5 → XP_006229072.3 mediator of RNA polymerase II transcription subunit 25 isoform X3

UniProtKB/TrEMBL

A0A8I6B1T5

Related

ENSRNOP00000112460.1, ENSRNOT00000159201.1

Conserved Domains (6) summary

pfam09770
Location:612 → 743

PAT1; Topoisomerase II-associated protein PAT1

pfam11232
Location:462 → 571

Med25; Mediator complex subunit 25 PTOV activation and synapsin 2

pfam11235
Location:289 → 445

Med25_SD1; Mediator complex subunit 25 synapsin 1

pfam11244
Location:683 → 739

Med25_NR-box; Mediator complex subunit 25 C-terminal NR box-containing

pfam11265
Location:73 → 287

Med25_VWA; Mediator complex subunit 25 von Willebrand factor type A

pfam18658
Location:754 → 812

zf-C2H2_12; Zinc-finger C2H2-type
XM_006229007.5 → XP_006229069.3 mediator of RNA polymerase II transcription subunit 25 isoform X1

UniProtKB/TrEMBL

A0A8I6AJG1, A0A8I6B1T5

Related

ENSRNOP00000093019.1, ENSRNOT00000105598.2

Conserved Domains (6) summary

pfam09770
Location:648 → 779

PAT1; Topoisomerase II-associated protein PAT1

pfam11232
Location:462 → 607

Med25; Mediator complex subunit 25 PTOV activation and synapsin 2

pfam11235
Location:289 → 445

Med25_SD1; Mediator complex subunit 25 synapsin 1

pfam11244
Location:719 → 775

Med25_NR-box; Mediator complex subunit 25 C-terminal NR box-containing

pfam11265
Location:73 → 287

Med25_VWA; Mediator complex subunit 25 von Willebrand factor type A

pfam18658
Location:790 → 848

zf-C2H2_12; Zinc-finger C2H2-type
XM_006229013.3 → XP_006229075.1 mediator of RNA polymerase II transcription subunit 25 isoform X9

UniProtKB/TrEMBL

D3ZJW8

Conserved Domains (6) summary

pfam09770
Location:484 → 615

PAT1; Topoisomerase II-associated protein PAT1

pfam11232
Location:298 → 443

Med25; Mediator complex subunit 25 PTOV activation and synapsin 2

pfam11235
Location:125 → 281

Med25_SD1; Mediator complex subunit 25 synapsin 1

pfam11244
Location:555 → 611

Med25_NR-box; Mediator complex subunit 25 C-terminal NR box-containing

pfam18658
Location:626 → 684

zf-C2H2_12; Zinc-finger C2H2-type

cl00057
Location:1 → 123

vWFA; Von Willebrand factor type A (vWA) domain was originally found in the blood coagulation protein von Willebrand factor (vWF). Typically, the vWA domain is made up of approximately 200 amino acid residues folded into a classic a/b para-rossmann type of ...
XM_063284073.1 → XP_063140143.1 mediator of RNA polymerase II transcription subunit 25 isoform X9

UniProtKB/TrEMBL

D3ZJW8
XM_039104904.2 → XP_038960832.1 mediator of RNA polymerase II transcription subunit 25 isoform X9

UniProtKB/TrEMBL

D3ZJW8

Conserved Domains (6) summary

pfam09770
Location:484 → 615

PAT1; Topoisomerase II-associated protein PAT1

pfam11232
Location:298 → 443

Med25; Mediator complex subunit 25 PTOV activation and synapsin 2

pfam11235
Location:125 → 281

Med25_SD1; Mediator complex subunit 25 synapsin 1

pfam11244
Location:555 → 611

Med25_NR-box; Mediator complex subunit 25 C-terminal NR box-containing

pfam18658
Location:626 → 684

zf-C2H2_12; Zinc-finger C2H2-type

cl00057
Location:1 → 123

vWFA; Von Willebrand factor type A (vWA) domain was originally found in the blood coagulation protein von Willebrand factor (vWF). Typically, the vWA domain is made up of approximately 200 amino acid residues folded into a classic a/b para-rossmann type of ...
XM_063284064.1 → XP_063140134.1 mediator of RNA polymerase II transcription subunit 25 isoform X7

UniProtKB/TrEMBL

A0A8I6B1T5
XM_039104896.2 → XP_038960824.1 mediator of RNA polymerase II transcription subunit 25 isoform X6

UniProtKB/TrEMBL

A0A8I6B1T5

Conserved Domains (4) summary

pfam11232
Location:461 → 606

Med25; Mediator complex subunit 25 PTOV activation and synapsin 2

pfam11235
Location:289 → 444

Med25_SD1; Mediator complex subunit 25 synapsin 1

pfam11244
Location:694 → 782

Med25_NR-box; Mediator complex subunit 25 C-terminal NR box-containing

pfam11265
Location:73 → 287

Med25_VWA; Mediator complex subunit 25 von Willebrand factor type A
XM_006229011.5 → XP_006229073.3 mediator of RNA polymerase II transcription subunit 25 isoform X4

UniProtKB/TrEMBL

A0A8I6B1T5

Conserved Domains (4) summary

pfam11232
Location:461 → 606

Med25; Mediator complex subunit 25 PTOV activation and synapsin 2

pfam11235
Location:289 → 444

Med25_SD1; Mediator complex subunit 25 synapsin 1

pfam11244
Location:718 → 806

Med25_NR-box; Mediator complex subunit 25 C-terminal NR box-containing

pfam11265
Location:73 → 287

Med25_VWA; Mediator complex subunit 25 von Willebrand factor type A
XM_063284065.1 → XP_063140135.1 mediator of RNA polymerase II transcription subunit 25 isoform X8

UniProtKB/TrEMBL

A0A8I6B1T5
XM_006229012.5 → XP_006229074.3 mediator of RNA polymerase II transcription subunit 25 isoform X5

UniProtKB/TrEMBL

A0A8I6B1T5

Conserved Domains (4) summary

pfam11232
Location:462 → 607

Med25; Mediator complex subunit 25 PTOV activation and synapsin 2

pfam11235
Location:289 → 445

Med25_SD1; Mediator complex subunit 25 synapsin 1

pfam11244
Location:695 → 783

Med25_NR-box; Mediator complex subunit 25 C-terminal NR box-containing

pfam11265
Location:73 → 287

Med25_VWA; Mediator complex subunit 25 von Willebrand factor type A
XM_063284077.1 → XP_063140147.1 mediator of RNA polymerase II transcription subunit 25 isoform X10