Kcnj1 potassium inwardly-rectifying channel, subfamily J, member 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Symbol: Kcnj1provided by RGD
Full Name: potassium inwardly-rectifying channel, subfamily J, member 1provided by RGD
Primary source: RGD:2957
See related: EnsemblRapid:ENSRNOG00000059005 AllianceGenome:RGD:2957
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Kcnj; KAB-1; ROMK1; ROMK2; ROMK3; kir1.1; Kcnj1_v1; Kcnj1_v3
Summary: Enables several functions, including ATP-activated inward rectifier potassium channel activity; anion binding activity; and potassium ion binding activity. Involved in several processes, including cellular response to magnesium ion; potassium ion import across plasma membrane; and response to potassium ion. Predicted to be part of monoatomic ion channel complex. Predicted to be active in plasma membrane. Biomarker of nephrotic syndrome. Human ortholog(s) of this gene implicated in Bartter disease type 2. Orthologous to human KCNJ1 (potassium inwardly rectifying channel subfamily J member 1). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Restricted expression toward (RPKM 575.3) See more
Orthologs: human mouse all
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Genomic context

Location:: 8q21

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	8	NC_086026.1 (39014822..39066716)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	8	NC_051343.1 (30779883..30808607)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	8	NC_005107.4 (33490280..33519127)

Chromosome 8 - NC_086026.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data suggest underlying pathology for some patients with type II Bartter syndrome is linked to stability of ROMK1 in ERAD pathway; using a yeast expression system, cells can be rescued by wild-type (rat) ROMK1 but not by ROMK1 containing any one of four mutations found in (human) type II Bartter syndrome; mutant ROMKs are significantly less stable than wild-type ROMK. (ERAD = endoplasmic reticulum-associated degradation)
Title: Endoplasmic reticulum-associated degradation of the renal potassium channel, ROMK, leads to type II Bartter syndrome.
Blockade of ROMK or Na(+)-K(+)-2Cl(-) transport inhibits tubuloglomerular feedback yet increases renal vascular resistance. The renal vasoconstriction was independent of volume depletion, blood pressure, TGF, or proximal tubule hydrostatic pressure.
Title: Inhibition of ROMK blocks macula densa tubuloglomerular feedback yet causes renal vasoconstriction in anesthetized rats.
These preliminary findings suggest that K channel genes play a role in the development of secondary lymphedema
Title: Potassium Channel Candidate Genes Predict the Development of Secondary Lymphedema Following Breast Cancer Surgery.
Data indicate that mutations in kir1.1 potassium channel preferentially increase pH sensitivity.
Title: State-dependent network connectivity determines gating in a K+ channel.
The TPNQ toxin-rKir1.1 channel complex structure not only revealed their unique interaction mechanism, but also elucidate the relative insensitivity of rKir1.1 channel towards animal toxins.
Title: Unique mechanism of the interaction between honey bee toxin TPNQ and rKir1.1 potassium channel explored by computational simulations: insights into the relative insensitivity of channel towards animal toxins.
Heterozygous disruption of renal outer medullary potassium channel in rats is associated with reduced blood pressure.
Title: Heterozygous disruption of renal outer medullary potassium channel in rats is associated with reduced blood pressure.
Low luminal potassium inhibits the activity of ROMK channels in the kidney cortical collecting duct.
Title: Inhibition of ROMK channels by low extracellular K+ and oxidative stress.
The findings support ROMK as the pore-forming subunit of the cytoprotective mitoK(ATP) channel in heart mitochondria.
Title: Mitochondrial ROMK channel is a molecular component of mitoK(ATP).
Insulin also more than doubled ROMK (tertiapin-Q-sensitive) K(+) currents in kidney collecting ducts.
Title: Effects of insulin on Na and K transporters in the rat CCD.
The large increase in the Rb/K conductance ratio, with no change in K/Na permeability or rectification, is consistent with R128Y-Kir1.1b causing a subtle change in the selectivity filter
Title: A conserved arginine near the filter of Kir1.1 controls Rb/K selectivity.

Submit: New GeneRIF Correction See all GeneRIFs (32)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATP binding	IDA Inferred from Direct Assay more info	PubMed
enables ATP-activated inward rectifier potassium channel activity	IBA Inferred from Biological aspect of Ancestor more info
enables ATP-activated inward rectifier potassium channel activity	IDA Inferred from Direct Assay more info	PubMed
enables inward rectifier potassium channel activity	IDA Inferred from Direct Assay more info	PubMed
enables inward rectifier potassium channel activity	IEA Inferred from Electronic Annotation more info
enables inward rectifier potassium channel activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables inward rectifier potassium channel activity	ISO Inferred from Sequence Orthology more info
enables peptide binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol-4,5-bisphosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol-4,5-bisphosphate binding	IEA Inferred from Electronic Annotation more info
enables phosphatidylinositol-4,5-bisphosphate binding	ISO Inferred from Sequence Orthology more info
enables potassium channel activity	ISO Inferred from Sequence Orthology more info
enables potassium ion binding	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in cellular response to magnesium ion	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within circulatory system development	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within gene expression	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within kidney development	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within post-embryonic development	ISO Inferred from Sequence Orthology more info
involved_in potassium ion import across plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
involved_in potassium ion import across plasma membrane	IEA Inferred from Electronic Annotation more info
involved_in potassium ion import across plasma membrane	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in potassium ion transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in potassium ion transport	IDA Inferred from Direct Assay more info	PubMed
involved_in potassium ion transport	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of G-protein activated inward rectifier potassium channel activity	ISO Inferred from Sequence Orthology more info
involved_in regulation of monoatomic ion transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of monoatomic ion transmembrane transport	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within renal sodium ion absorption	ISO Inferred from Sequence Orthology more info
involved_in response to potassium ion	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within tissue homeostasis	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
part_of monoatomic ion channel complex	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: ATP-sensitive inward rectifier potassium channel 1

Names: ATP-regulated potassium channel ROM-K; K+ channel protein; inward rectifier K(+) channel Kir1.1; potassium channel, inwardly rectifying subfamily J member 1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001309297.1 → NP_001296226.1 ATP-sensitive inward rectifier potassium channel 1 isoform 2

See identical proteins and their annotated locations for NP_001296226.1

Status: VALIDATED

Description

Transcript Variant: This variant (2, also known as ROMK2) contains an alternate exon in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 2 which has a shorter N-terminus compared to isoform 1.

Source sequence(s)

JAXUCZ010000008

UniProtKB/Swiss-Prot

O88639, O88640, P35560, Q9QUR5

UniProtKB/TrEMBL

A0A8I6AW52, A6JYI0

Related

ENSRNOP00000077878.2, ENSRNOT00000110096.2

Conserved Domains (1) summary

pfam01007
Location:24 → 352

IRK; Inward rectifier potassium channel
NM_001309298.1 → NP_001296227.1 ATP-sensitive inward rectifier potassium channel 1 isoform 3

See identical proteins and their annotated locations for NP_001296227.1

Status: VALIDATED

Description

Transcript Variant: This variant (3, also known as ROMK6.1) contains two alternate exons in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 3 which has a shorter N-terminus compared to isoform 1. Variants 3, 4 and 5 encode the same protein (isoform 3).

Source sequence(s)

JAXUCZ010000008

UniProtKB/TrEMBL

A0A0G2K298, A6JYI4

Related

ENSRNOP00000072157.2, ENSRNOT00000081525.3

Conserved Domains (1) summary

cl21560
Location:1 → 198

Ion_trans_2; Ion channel
NM_001309299.1 → NP_001296228.1 ATP-sensitive inward rectifier potassium channel 1 isoform 3

See identical proteins and their annotated locations for NP_001296228.1

Status: VALIDATED

Description

Transcript Variant: This variant (4, also known as ROMK3.1) contains three alternate exons in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 3 which has a shorter N-terminus compared to isoform 1. Variants 3, 4 and 5 encode the same protein (isoform 3).

Source sequence(s)

JAXUCZ010000008

UniProtKB/TrEMBL

A0A0G2K298, A6JYI4

Related

ENSRNOP00000078843.2, ENSRNOT00000099372.2

Conserved Domains (1) summary

cl21560
Location:1 → 198

Ion_trans_2; Ion channel
NM_001309301.1 → NP_001296230.1 ATP-sensitive inward rectifier potassium channel 1 isoform 3

See identical proteins and their annotated locations for NP_001296230.1

Status: VALIDATED

Description

Transcript Variant: This variant (5, also known as ROMK1.1) contains an alternate exon in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 3 which has a shorter N-terminus compared to isoform 1. Variants 3, 4 and 5 encode the same protein (isoform 3).

Source sequence(s)

JAXUCZ010000008

UniProtKB/TrEMBL

A0A0G2K298, A6JYI4

Conserved Domains (1) summary

cl21560
Location:1 → 198

Ion_trans_2; Ion channel
NM_017023.2 → NP_058719.2 ATP-sensitive inward rectifier potassium channel 1 isoform 1

See identical proteins and their annotated locations for NP_058719.2

Status: VALIDATED

Description

Transcript Variant: This variant (1, also known as ROMK1) represents the longest transcript and encodes the longest isoform (1).

Source sequence(s)

JAXUCZ010000008

UniProtKB/Swiss-Prot

O88639, O88640, P35560, Q9QUR5

UniProtKB/TrEMBL

A0A0G2JYW2, A6JYH9

Related

ENSRNOP00000070813.1, ENSRNOT00000081614.3

Conserved Domains (1) summary

pfam01007
Location:43 → 371

IRK; Inward rectifier potassium channel

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086026.1 Reference GRCr8

Range

39014822..39066716

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063264896.1 → XP_063120966.1 ATP-sensitive inward rectifier potassium channel 1 isoform X1

UniProtKB/TrEMBL

A0A8I5ZLP0
XM_063264899.1 → XP_063120969.1 ATP-sensitive inward rectifier potassium channel 1 isoform X3
XM_063264898.1 → XP_063120968.1 ATP-sensitive inward rectifier potassium channel 1 isoform X2

UniProtKB/Swiss-Prot

O88639, O88640, P35560, Q9QUR5

UniProtKB/TrEMBL

A0A8I5ZJL6
XM_063264897.1 → XP_063120967.1 ATP-sensitive inward rectifier potassium channel 1 isoform X2

UniProtKB/Swiss-Prot

O88639, O88640, P35560, Q9QUR5

UniProtKB/TrEMBL

A0A8I5ZJL6