Cul5 cullin 5 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Cul5provided by RGD
Official Full Name: cullin 5provided by RGD
Primary source: RGD:621742
See related: EnsemblRapid:ENSRNOG00000008039 AllianceGenome:RGD:621742
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Predicted to enable ubiquitin ligase complex scaffold activity and ubiquitin protein ligase binding activity. Involved in regulation of cytosolic calcium ion concentration. Predicted to be located in site of DNA damage. Predicted to be part of Cul5-RING ubiquitin ligase complex and SCF ubiquitin ligase complex. Predicted to be active in nucleus. Orthologous to human CUL5 (cullin 5). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Muscle (RPKM 331.2), Heart (RPKM 224.9) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 8q24

Exon count:: 19

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	8	NC_086026.1 (62909150..62974641, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	8	NC_051343.1 (54012963..54066751, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	8	NC_005107.4 (58199992..58255149, complement)

Chromosome 8 - NC_086026.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

the data demonstrate that VACM-1 is involved in the regulation of AQP2 protein concentration and may play a role in regulating water balance.
Title: Aquaporin-2 levels in vitro and in vivo are regulated by VACM-1, a cul 5 gene.
VACM-1 mRNA and protein levels were significantly higher in rat mesenteric arteries, skeletal muscle and the heart ventricle but not in the heart atrium from 24-h water-deprived rats when compared to the controls.
Title: VACM-1/cul5 expression in vascular tissue in vivo is induced by water deprivation and its expression in vitro regulates aquaporin-1 concentrations.
The results suggest that the antiproliferative effect of VACM-1/Cul5 is dependent on its posttranslational modifications and will help in the design of new anticancer therapeutics that target the Nedd8 pathway.
Title: Phosphorylation of VACM-1/Cul5 by protein kinase A regulates its neddylation and antiproliferative effect.
Results show that VACM-1 protein regulates endothelial cell growth and may modulate angiogenesis by a mechanism that involves MAPK phosphorylation and maspin expression.
Title: Expression of VACM-1/cul5 mutant in endothelial cells induces MAPK phosphorylation and maspin degradation and converts cells to the angiogenic phenotype.
Widespread and ubiquitous expression of Cul-5 in the brain suggests that it may have a vital role(s) in cellular activities of the CNS.
Title: Cullin-5 is ubiquitous in the rat brain.
Cullin 5 may have a role in CNS cell fate determination under conditions of traumatic stress
Title: Cullin 5 gene expression in the rat cerebral cortex and hippocampus following traumatic brain injury (TBI).
Cullin-5 mRNA levels are significantly increased in the rat cerebral cortex, hypothalamus and kidney following 48 h of water deprivation.
Title: Osmotic stress increases cullin-5 (cul-5) mRNA in the rat cerebral cortex, hypothalamus and kidney.
transcript encoding vasopressin-activated calcium-mobilizing (VACM-1) receptor appears to be ubiquitously expressed in various tissues of the laboratory rat
Title: Vasopressin-activated calcium-mobilizing (VACM-1) receptor mRNA is present in peripheral organs and the central nervous system of the laboratory rat.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH141279 (e-PCR)
- UniSTS:223275

D8Rat111 (e-PCR), detects polymorphism
- UniSTS:228213

RH130898 (e-PCR)
- UniSTS:214182

BF397556 (e-PCR)
- UniSTS:246228

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables protein-macromolecule adaptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin ligase complex scaffold activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin ligase complex scaffold activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin ligase complex scaffold activity	ISS Inferred from Sequence or Structural Similarity more info
enables ubiquitin protein ligase binding	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase binding	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin-protein transferase activity	TAS Traceable Author Statement more info	PubMed
enables vasopressin receptor activity	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
involved_in G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within cerebral cortex radially oriented cell migration	ISO Inferred from Sequence Orthology more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IEA Inferred from Electronic Annotation more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in protein K11-linked ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in protein K11-linked ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein polyubiquitination	TAS Traceable Author Statement more info	PubMed
involved_in protein ubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within protein ubiquitination	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within radial glia guided migration of Purkinje cell	ISO Inferred from Sequence Orthology more info
acts_upstream_of_negative_effect reelin-mediated signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_negative_effect reelin-mediated signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of cytosolic calcium ion concentration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of neuron migration	ISO Inferred from Sequence Orthology more info
involved_in regulation of neuron migration	ISS Inferred from Sequence or Structural Similarity more info
involved_in ubiquitin-dependent protein catabolic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of Cul5-RING ubiquitin ligase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Cul5-RING ubiquitin ligase complex	IEA Inferred from Electronic Annotation more info
part_of Cul5-RING ubiquitin ligase complex	ISO Inferred from Sequence Orthology more info
part_of Cul5-RING ubiquitin ligase complex	ISS Inferred from Sequence or Structural Similarity more info
part_of Cul5-RING ubiquitin ligase complex	TAS Traceable Author Statement more info	PubMed
part_of SCF ubiquitin ligase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of cullin-RING ubiquitin ligase complex	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	ISO Inferred from Sequence Orthology more info
located_in site of DNA damage	IEA Inferred from Electronic Annotation more info
located_in site of DNA damage	ISO Inferred from Sequence Orthology more info
located_in site of DNA damage	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: cullin-5

Names: CUL-5; VACM-1; vasopressin-activated calcium-mobilizing receptor 1; vasopressin-activated calcium-mobilizing receptor protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_022683.1 → NP_073174.1 cullin-5

See identical proteins and their annotated locations for NP_073174.1

Status: PROVISIONAL

Source sequence(s)

AF135115

UniProtKB/Swiss-Prot

Q9JJ31

UniProtKB/TrEMBL

A0A8I6A7N3

Related

ENSRNOP00000010956.4, ENSRNOT00000010956.6

Conserved Domains (3) summary

smart00182
Location:443 → 586

CULLIN; Cullin

pfam00888
Location:20 → 671

Cullin; Cullin family

pfam10557
Location:713 → 772

Cullin_Nedd8; Cullin protein neddylation domain

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086026.1 Reference GRCr8

Range

62909150..62974641 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006243090.5 → XP_006243152.1 cullin-5 isoform X1

UniProtKB/TrEMBL

A0A0H2UHG3, A0A8I6ASH3, A6J4J8

Related

ENSRNOP00000097338.1, ENSRNOT00000114900.2

Conserved Domains (3) summary

smart00182
Location:491 → 634

CULLIN; Cullin

pfam00888
Location:95 → 719

Cullin; Cullin family

pfam10557
Location:761 → 820

Cullin_Nedd8; Cullin protein neddylation domain