Rasgrf1 RAS protein-specific guanine nucleotide-releasing factor 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Rasgrf1provided by RGD
Official Full Name: RAS protein-specific guanine nucleotide-releasing factor 1provided by RGD
Primary source: RGD:620395
See related: EnsemblRapid:ENSRNOG00000014025 AllianceGenome:RGD:620395
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: GNRP; P140 RAS-GRF
Summary: Enables receptor tyrosine kinase binding activity and small GTPase binding activity. Involved in several processes, including activation of GTPase activity; modulation of chemical synaptic transmission; and positive regulation of Ras protein signal transduction. Located in several cellular components, including apical dendrite; apicolateral plasma membrane; and basolateral plasma membrane. Is active in glutamatergic synapse and postsynapse. Biomarker of fetal alcohol spectrum disorder. Orthologous to human RASGRF1 (Ras protein specific guanine nucleotide releasing factor 1). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Restricted expression toward (RPKM 361.8) See more
Orthologs: human mouse all
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Genomic context

Location:: 8q31

Exon count:: 29

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	8	NC_086026.1 (99324986..99454099)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	8	NC_051343.1 (90445154..90574274)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	8	NC_005107.4 (97277250..97405095)

Chromosome 8 - NC_086026.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

our data indicate that superoxide production is induced by the NR2B-NMDARs/RasGRF1/NOX2 pathway and promotes dendritogenesis.
Title: Superoxide generation via the NR2B-NMDAR/RasGRF1/NOX2 pathway promotes dendritogenesis.
Novel mercaptoacetamide-based class II histone deacetylase inhibitor HDACIW2 treatment regulated the levels of RasGRF1 and p-ERK, and that W2 requires RasGRF1 and ERK signaling to alter dendritic spine formation, suggesting that W2 regulates dendritic spine formation via a RasGRF1/ERK-dependent signaling pathway
Title: A Mercaptoacetamide-Based Class II Histone Deacetylase Inhibitor Increases Dendritic Spine Density via RasGRF1/ERK Pathway.
VLDLR requires RasGRF1/CaMKII to alter dendritic spine formation.
Title: Very low density lipoprotein receptor regulates dendritic spine formation in a RasGRF1/CaMKII dependent manner.
Ras-GRF1 expression in epileptic rats was already reduced at 1 day post-seizures, then gradually decreased during the latent period and reached a minimum level during the chronic phase.
Title: Decreased expression of Ras-GRF1 in the brain tissue of the intractable epilepsy patients and experimental rats.
Dominated NR2A expression and the association between NR1NR2B-receptors and RasGRF1 may contribute to dendritic branch formation in the rat.
Title: Differential roles of NMDA Receptor Subtypes NR2A and NR2B in dendritic branch development and requirement of RasGRF1.
These results strongly implicate Ras-GRF1 in the integration of the two main neurotransmitter inputs to the striatum and in the maladaptive modulation of striatal networks in response to cocaine.
Title: Ras-guanine nucleotide-releasing factor 1 (Ras-GRF1) controls activation of extracellular signal-regulated kinase (ERK) signaling in the striatum and long-term behavioral responses to cocaine.
data identify the Ras activator, Ras-GRF1, although not Ras-GRF2, as a susceptible target to cocaine stimulation in striatal neurons
Title: Cocaine increases Ras-guanine nucleotide-releasing factor 1 protein expression in the rat striatum in vivo.
RasGRF family exchange factors, both endogenous and ectopically expressed, are present in the endoplasmic reticulum but not in the Golgi complex
Title: Activation of H-Ras in the endoplasmic reticulum by the RasGRF family guanine nucleotide exchange factors.
N-terminal region of both proteins binds to the scaffold protein IB2/JIP2. IB2/JIP2 is a scaffold for the p38 mitogen-activated protein (MAP) kinase cascade because it binds to the Rac target MLK3.
Title: Interaction of Rac exchange factors Tiam1 and Ras-GRF1 with a scaffold for the p38 mitogen-activated protein kinase cascade.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D8Rat20 (e-PCR)
- UniSTS:119088

AU049054 (e-PCR)
- UniSTS:145502

AU026641 (e-PCR)
- UniSTS:114081

RH132198 (e-PCR)
- UniSTS:215481

STS-L26584 (e-PCR)
- UniSTS:14301

RH133787 (e-PCR)
- UniSTS:217070

D8Got142 (e-PCR)
- UniSTS:112622

AU046538 (e-PCR)
- UniSTS:111355

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables GTPase activator activity	TAS Traceable Author Statement more info	PubMed
enables glutamate receptor binding	IEA Inferred from Electronic Annotation more info
enables glutamate receptor binding	ISO Inferred from Sequence Orthology more info
enables guanyl-nucleotide exchange factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables guanyl-nucleotide exchange factor activity	IEA Inferred from Electronic Annotation more info
enables guanyl-nucleotide exchange factor activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables receptor tyrosine kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables small GTPase binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in Ras protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in activation of GTPase activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in activation of GTPase activity	ISO Inferred from Sequence Orthology more info
involved_in activation of GTPase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in cellular response to antibiotic	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to insulin stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in neuron projection development	IEA Inferred from Electronic Annotation more info
involved_in neuron projection development	ISO Inferred from Sequence Orthology more info
involved_in neuron projection development	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in positive regulation of GTPase activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of GTPase activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of GTPase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in positive regulation of Ras protein signal transduction	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of fibroblast proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in postsynaptic modulation of chemical synaptic transmission	EXP Inferred from Experiment more info	PubMed
involved_in postsynaptic modulation of chemical synaptic transmission	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of NMDA receptor activity	ISO Inferred from Sequence Orthology more info
involved_in regulation of Rac protein signal transduction	IEA Inferred from Electronic Annotation more info
involved_in regulation of Rac protein signal transduction	ISO Inferred from Sequence Orthology more info
involved_in regulation of Rac protein signal transduction	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in regulation of Ras protein signal transduction	IEA Inferred from Electronic Annotation more info
involved_in regulation of Ras protein signal transduction	ISO Inferred from Sequence Orthology more info
involved_in regulation of Ras protein signal transduction	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in regulation of neuronal synaptic plasticity	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of neuronal synaptic plasticity	ISO Inferred from Sequence Orthology more info
involved_in regulation of synaptic plasticity	IDA Inferred from Direct Assay more info	PubMed
involved_in response to amphetamine	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to endoplasmic reticulum stress	IEA Inferred from Electronic Annotation more info
involved_in response to endoplasmic reticulum stress	ISO Inferred from Sequence Orthology more info
involved_in small GTPase-mediated signal transduction	IEA Inferred from Electronic Annotation more info
involved_in type B pancreatic cell proliferation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within type B pancreatic cell proliferation	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in apical dendrite	IDA Inferred from Direct Assay more info	PubMed
located_in apicolateral plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in basolateral plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISS Inferred from Sequence or Structural Similarity more info	PubMed
is_active_in glutamatergic synapse	EXP Inferred from Experiment more info	PubMed
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
located_in growth cone	IEA Inferred from Electronic Annotation more info
located_in growth cone	ISO Inferred from Sequence Orthology more info
located_in growth cone	ISS Inferred from Sequence or Structural Similarity more info	PubMed
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	TAS Traceable Author Statement more info
is_active_in postsynapse	EXP Inferred from Experiment more info	PubMed
is_active_in postsynapse	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: ras-specific guanine nucleotide-releasing factor 1

Names: guanine nucleotide-releasing protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001105753.1 → NP_001099223.1 ras-specific guanine nucleotide-releasing factor 1 isoform 2

See identical proteins and their annotated locations for NP_001099223.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate splice pattern in the 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (2) has a substantially shorter and distinct C-terminus, compared to isoform 1.

Source sequence(s)

CH473954

UniProtKB/TrEMBL

A0A8I5Y148, A0A8I6AEJ3, A6I210

Related

ENSRNOP00000077058.1, ENSRNOT00000106070.2

Conserved Domains (2) summary

pfam00169
Location:24 → 82

PH; PH domain

cl17171
Location:19 → 93

PH-like; Pleckstrin homology-like domain
NM_001170531.1 → NP_001164002.1 ras-specific guanine nucleotide-releasing factor 1 isoform 1

See identical proteins and their annotated locations for NP_001164002.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

BC091317, BF405809, CB583610, CB607130, CB713048, CB714920

UniProtKB/Swiss-Prot

P28818

UniProtKB/TrEMBL

A6I209

Related

ENSRNOP00000111797.1, ENSRNOT00000160336.1

Conserved Domains (6) summary

smart00147
Location:1005 → 1241

RasGEF; Guanine nucleotide exchange factor for Ras-like small GTPases

smart00325
Location:244 → 425

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases

pfam00169
Location:24 → 129

PH; PH domain

pfam00618
Location:632 → 680

RasGEF_N; RasGEF N-terminal motif

cl02520
Location:909 → 978

REM; Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also called REM domain (Ras exchanger motif). This domain is common in nucleotide exchange factors for Ras-like small GTPases and is typically found immediately ...

cl17171
Location:19 → 154

PH-like; Pleckstrin homology-like domain