Cyb5r3 cytochrome b5 reductase 3 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Cyb5r3provided by RGD
Official Full Name: cytochrome b5 reductase 3provided by RGD
Primary source: RGD:2502
See related: EnsemblRapid:ENSRNOG00000009592 AllianceGenome:RGD:2502
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Dia1; Nadhcb5; RNNADHCB5
Summary: Enables cytochrome-b5 reductase activity, acting on NAD(P)H and nucleotide binding activity. Predicted to be involved in nitric oxide biosynthetic process. Located in endoplasmic reticulum membrane and mitochondrial outer membrane. Human ortholog(s) of this gene implicated in breast cancer and methemoglobinemia. Orthologous to human CYB5R3 (cytochrome b5 reductase 3). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Liver (RPKM 562.3), Lung (RPKM 375.6) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 7q34

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	7	NC_086025.1 (116186729..116204290, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	7	NC_051342.1 (114306686..114324247, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	7	NC_005106.4 (124024003..124041564, complement)

Chromosome 7 - NC_086025.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

results highlight the importance of CYB5R3 and NQO1, two NAD(+) producers, for the promotion of health and extended lifespan
Title: Overexpression of CYB5R3 and NQO1, two NAD(+) -producing enzymes, mimics aspects of caloric restriction.
Results point out the enrichment of cytochrome b 5 reductase isoform 3/cytochrome b 5 system in neuronal cells and structures of the cerebellum and brain stem.
Title: High expression of cytochrome b 5 reductase isoform 3/cytochrome b 5 system in the cerebellum and pyramidal neurons of adult rat brain.
The data obtained argue against VDAC1 and in favor of Cyb5R3 involvement in the activation of redox cyclers in the outer mitochondrial membrane.
Title: External mitochondrial NADH-dependent reductase of redox cyclers: VDAC1 or Cyb5R3?
Heterologous expression of an endogenous rat cytochrome b(5)/cytochrome b(5) reductase fusion protein
Title: Heterologous expression of an endogenous rat cytochrome b(5)/cytochrome b(5) reductase fusion protein: identification of histidines 62 and 85 as the heme axial ligands.
Structure of cytochrome b5 reductase S127P mutant reveals that the adenosine diphosphate (ADP) moiety of the FAD prosthetic group is displaced into the corresponding ADP binding site of the physiologic substrate NADH, thus acting as a substrate inhibitor.
Title: The structure of the S127P mutant of cytochrome b5 reductase that causes methemoglobinemia shows the AMP moiety of the flavin occupying the substrate binding site.

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General gene information

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Markers

RH137308 (e-PCR)
- UniSTS:219396

5STS03 (e-PCR)
- UniSTS:503890

D7Wox30 (e-PCR)
- UniSTS:517852

Cyb5r3 (e-PCR), detects polymorphism
- UniSTS:499178

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ADP binding	IDA Inferred from Direct Assay more info	PubMed
enables AMP binding	IDA Inferred from Direct Assay more info	PubMed
enables FAD binding	IBA Inferred from Biological aspect of Ancestor more info
enables FAD binding	IDA Inferred from Direct Assay more info	PubMed
enables FAD binding	ISO Inferred from Sequence Orthology more info
enables NAD binding	IDA Inferred from Direct Assay more info	PubMed
enables cytochrome-b5 reductase activity, acting on NAD(P)H	IDA Inferred from Direct Assay more info	PubMed
enables cytochrome-b5 reductase activity, acting on NAD(P)H	IEA Inferred from Electronic Annotation more info
enables cytochrome-b5 reductase activity, acting on NAD(P)H	ISO Inferred from Sequence Orthology more info
enables cytochrome-b5 reductase activity, acting on NAD(P)H	TAS Traceable Author Statement more info	PubMed
enables flavin adenine dinucleotide binding	IDA Inferred from Direct Assay more info	PubMed
enables flavin adenine dinucleotide binding	ISO Inferred from Sequence Orthology more info
contributes_to nitrite reductase (NO-forming) activity	ISO Inferred from Sequence Orthology more info
enables oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in biological_process	ND No biological Data available more info
involved_in cholesterol biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in nitric oxide biosynthetic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum membrane	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
is_active_in endoplasmic reticulum membrane	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum membrane	ISO Inferred from Sequence Orthology more info
located_in lipid droplet	ISO Inferred from Sequence Orthology more info
located_in mitochondrial membrane	ISO Inferred from Sequence Orthology more info
located_in mitochondrial outer membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial outer membrane	IEA Inferred from Electronic Annotation more info
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	ISO Inferred from Sequence Orthology more info	PubMed
part_of nitric-oxide synthase complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: NADH-cytochrome b5 reductase 3

Names: B5R; Diaphorase (NADH) (cytochrome b-5 reductase); diaphorase-1

NP_620232.1

EC 1.6.2.2

XP_006242127.1

EC 1.6.2.2

XP_038934366.1

EC 1.6.2.2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_138877.1 → NP_620232.1 NADH-cytochrome b5 reductase 3

See identical proteins and their annotated locations for NP_620232.1

Status: PROVISIONAL

Source sequence(s)

D00636

UniProtKB/Swiss-Prot

P20070, Q64569, Q64720

UniProtKB/TrEMBL

A0A8L2QWB9, A6HT85

Related

ENSRNOP00000061381.5, ENSRNOT00000064700.5

Conserved Domains (2) summary

cd06183
Location:45 → 301

cyt_b5_reduct_like; Cytochrome b5 reductase catalyzes the reduction of 2 molecules of cytochrome b5 using NADH as an electron donor. Like ferredoxin reductases, these proteins have an N-terminal FAD binding subdomain and a C-terminal NADH binding subdomain, separated by a ...

PTZ00319
Location:16 → 301

PTZ00319; NADH-cytochrome B5 reductase; Provisional

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086025.1 Reference GRCr8

Range

116186729..116204290 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006242065.5 → XP_006242127.1 NADH-cytochrome b5 reductase 3 isoform X1

UniProtKB/TrEMBL

A0A8L2QWB9

Conserved Domains (2) summary

cd06183
Location:51 → 307

cyt_b5_reduct_like; Cytochrome b5 reductase catalyzes the reduction of 2 molecules of cytochrome b5 using NADH as an electron donor. Like ferredoxin reductases, these proteins have an N-terminal FAD binding subdomain and a C-terminal NADH binding subdomain, separated by a ...

PTZ00319
Location:22 → 307

PTZ00319; NADH-cytochrome B5 reductase; Provisional
XM_039078438.2 → XP_038934366.1 NADH-cytochrome b5 reductase 3 isoform X2

UniProtKB/TrEMBL

A0A8L2QWB9

Related

ENSRNOP00000082175.2, ENSRNOT00000111302.2

Conserved Domains (1) summary

PLN02252
Location:15 → 278

PLN02252; nitrate reductase [NADPH]