Pf4 platelet factor 4 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Pf4provided by RGD
Official Full Name: platelet factor 4provided by RGD
Primary source: RGD:3305
See related: EnsemblRapid:ENSRNOG00000028015 AllianceGenome:RGD:3305
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: PF-4; Pf4a; Cxcl4; RATPF4A
Summary: Predicted to enable CXCR3 chemokine receptor binding activity; chemokine activity; and heparin binding activity. Involved in several processes, including negative regulation of T-helper 17 cell differentiation; negative regulation of inflammatory response; and response to aldosterone. Located in platelet alpha granule. Part of protein-containing complex. Biomarker of acute myocardial infarction; anti-basement membrane glomerulonephritis; diabetic retinopathy; and retinal degeneration. Human ortholog(s) of this gene implicated in multiple myeloma. Orthologous to several human genes including PF4 (platelet factor 4). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Spleen (RPKM 767.8), Lung (RPKM 160.8) and 4 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 14p22

Exon count:: 3

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	14	NC_086032.1 (17582477..17583392, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	14	NC_051349.1 (17298304..17299220, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	14	NC_005113.4 (18848549..18849258, complement)

Chromosome 14 - NC_086032.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

PF4 induces inflammatory response through NF-kB signal pathway in rats with intracerebral haemorrhage.
Title: PF4 induces inflammatory response through NF-kB signal pathway in rats with intracerebral haemorrhage.
identified the informative Chronic Liver Allograft Dysfunction (CLAD)-associated genes; CXCL4, CXCR3, EGFR, JAK2, STAT3, and Collagen IV were associated with CLAD pathogenesis; validated that CXCL4 is upstream of these informative genes in the isolated hepatic stellate cells
Title: CXCL4 Contributes to the Pathogenesis of Chronic Liver Allograft Dysfunction.
The levels of PF-4 (as a platelet marker) mRNA were significantly higher in FeCl(2)-VT (2(-DeltaDeltaCT)=7.8) than in stasis-VT (2(-DeltaDeltaCT)=4.2, p<0.05).
Title: Quantification of platelet composition in experimental venous thrombosis by real-time polymerase chain reaction.
PF4 promoters are transactivated by USF1 and 2 which may play an important role in megakaryocytic gene expression
Title: Upstream stimulatory factors stimulate transcription through E-box motifs in the PF4 gene in megakaryocytes.
A carboxyl-terminal fragment of this protein effects a rapid histamine release from rat peritoneal mast cells.
Title: Platelet factor 4 fragment induces histamine release from rat peritoneal mast cells.
this study identified a novel regulatory element,TME(tandem repeat of MEIS1 binding element)in the PF4 promoter and demonstrated that MEIS1/PBX complexes bind to the TME
Title: Homeodomain proteins MEIS1 and PBXs regulate the lineage-specific transcription of the platelet factor 4 gene.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D14Arb3 (e-PCR), detects polymorphism
- UniSTS:226597

d14mgh10 (e-PCR)
- UniSTS:120183

D14Wox18 (e-PCR)
- UniSTS:517810

RH129105 (e-PCR)
- UniSTS:212413

Pf4 (e-PCR), detects polymorphism
- UniSTS:516554

Pf4 (e-PCR), detects polymorphism
- UniSTS:516595

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables CXCR chemokine receptor binding	IBA Inferred from Biological aspect of Ancestor more info
enables CXCR3 chemokine receptor binding	ISO Inferred from Sequence Orthology more info
enables CXCR3 chemokine receptor binding	ISS Inferred from Sequence or Structural Similarity more info
enables chemokine activity	IBA Inferred from Biological aspect of Ancestor more info
enables chemokine activity	ISO Inferred from Sequence Orthology more info
enables heparin binding	ISO Inferred from Sequence Orthology more info
enables heparin binding	ISS Inferred from Sequence or Structural Similarity more info
enables receptor ligand activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in adenylate cyclase-activating G protein-coupled receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in adenylate cyclase-activating G protein-coupled receptor signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in antimicrobial humoral immune response mediated by antimicrobial peptide	IBA Inferred from Biological aspect of Ancestor more info
involved_in antimicrobial humoral immune response mediated by antimicrobial peptide	ISO Inferred from Sequence Orthology more info
involved_in cellular response to lipopolysaccharide	IBA Inferred from Biological aspect of Ancestor more info
involved_in cytokine-mediated signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in cytokine-mediated signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in defense response to protozoan	ISO Inferred from Sequence Orthology more info
involved_in immune response	IDA Inferred from Direct Assay more info	PubMed
involved_in inflammatory response	IBA Inferred from Biological aspect of Ancestor more info
involved_in leukocyte chemotaxis	ISO Inferred from Sequence Orthology more info
involved_in leukocyte chemotaxis	ISS Inferred from Sequence or Structural Similarity more info
involved_in monocyte chemotaxis	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of MHC class II biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of T-helper 17 cell differentiation	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of angiogenesis	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of angiogenesis	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of blood coagulation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of extrinsic apoptotic signaling pathway in absence of ligand	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of inflammatory response	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of megakaryocyte differentiation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of megakaryocyte differentiation	ISS Inferred from Sequence or Structural Similarity more info
involved_in neutrophil chemotaxis	IBA Inferred from Biological aspect of Ancestor more info
involved_in osteoclast differentiation	HEP more info	PubMed
involved_in platelet activation	ISO Inferred from Sequence Orthology more info
involved_in platelet activation	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of macrophage derived foam cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of macrophage differentiation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of tumor necrosis factor production	ISO Inferred from Sequence Orthology more info
involved_in protein-containing complex assembly	IPI Inferred from Physical Interaction more info	PubMed
involved_in regulation of cell population proliferation	ISO Inferred from Sequence Orthology more info
involved_in regulation of cell population proliferation	ISS Inferred from Sequence or Structural Similarity more info
involved_in response to aldosterone	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info
is_active_in extracellular space	ISO Inferred from Sequence Orthology more info
located_in platelet alpha granule	IDA Inferred from Direct Assay more info	PubMed
part_of protein-containing complex	IDA Inferred from Direct Assay more info	PubMed
located_in vesicle	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: platelet factor 4

Names: C-X-C motif chemokine 4; chemokine (C-X-C motif) ligand 4

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001007729.2 → NP_001007730.1 platelet factor 4 precursor

See identical proteins and their annotated locations for NP_001007730.1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000014

UniProtKB/Swiss-Prot

P06765

UniProtKB/TrEMBL

A6KKE3

Related

ENSRNOP00000032276.1, ENSRNOT00000033406.3

Conserved Domains (1) summary

cd00273
Location:41 → 104

Chemokine_CXC; Chemokine_CXC: 1 of 4 subgroup designations based on the arrangement of the two N-terminal cysteine residues; includes a number of secreted growth factors and interferons involved in mitogenic, chemotactic, and inflammatory activity; many members ...