Pick1 protein interacting with PRKCA 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Pick1provided by RGD
Official Full Name: protein interacting with PRKCA 1provided by RGD
Primary source: RGD:69437
See related: EnsemblRapid:ENSRNOG00000011507 AllianceGenome:RGD:69437
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Prkcabp
Summary: Enables several functions, including Arp2/3 complex binding activity; SNAP receptor activity; and glutamate receptor binding activity. Involved in several processes, including dendritic spine maintenance; long-term synaptic depression; and regulation of receptor internalization. Located in dendritic spine; perinuclear region of cytoplasm; and postsynaptic density. Part of protein-containing complex. Is active in several cellular components, including glutamatergic synapse; postsynaptic endocytic zone; and synaptic vesicle. Orthologous to human PICK1 (protein interacting with PRKCA 1). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Testes (RPKM 80.2), Brain (RPKM 65.2) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 7q34

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	7	NC_086025.1 (112676890..112697275)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	7	NC_051342.1 (110796623..110816850)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	7	NC_005106.4 (120465472..120484824)

Chromosome 7 - NC_086025.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

multivalent protein-protein and protein-lipid interactions provide critical avidity for the strong binding between the PDZ domain scaffold proteins, PICK1 and PSD-95, and their cognate transmembrane binding partners
Title: Mechanisms of PDZ domain scaffold assembly illuminated by use of supported cell membrane sheets.
Study shows that oxygen and glucose deprivation (OGD) causes endocytosis, lysosomal targeting and consequent degradation of GluA2- and GluA3-containing AMPARs, and that PICK1 is required for both OGD-induced GluA2 endocytosis and lysosomal sorting. GluA1-containing AMPARs resist OGD-induced endocytosis and PICK1 binding to the endocytic adaptor AP2 is enhanced by OGD in hippocampal, but not cortical neurons.
Title: Endocytosis and lysosomal degradation of GluA2/3 AMPARs in response to oxygen/glucose deprivation in hippocampal but not cortical neurons.
study found that both GluA2 and PICK1 were down-regulated in kainate-treated rats for as long as eight weeks after induction of epilepsy; statistical analysis indicates that the functional role of PIC 1 in studied material is to increase GluA2 concentrations in the cells
Title: PICK1 facilitates lasting reduction in GluA2 concentration in the hippocampus during chronic epilepsy.
Results suggest that the interacting proteins PICK1 and N-ethylmaleimide sensitive fusion protein act as regulators of presynaptic GluA2-containing AMPAR trafficking between the active zone and a vesicle pool that may provide the basis of presynaptic components of synaptic plasticity.
Title: Presynaptic PICK1 facilitates trafficking of AMPA-receptors between active zone and synaptic vesicle pool.
PICK1 is a cargo-specific endocytic accessory protein required for efficient, activity-dependent AMPAR endocytosis.
Title: PICK1 regulates AMPA receptor endocytosis via direct interactions with AP2 α-appendage and dynamin.
This study defines a novel mechanism whereby elevated [Ca(2+)] induced by NMDA receptor activation modulates Ago2 and miRNA activity via PICK1. Our work suggests a Ca(2+)-dependent process to regulate miRNA activity in neurons in response to the induction of long-term depression.
Title: The PICK1 Ca(2+) sensor modulates N-methyl-d-aspartate (NMDA) receptor-dependent microRNA-mediated translational repression in neurons.
syntabulin forms a complex with PICK1 and ASICs, regulates ASIC protein expression in neurons, and participates in ASIC-induced acidotoxicity.
Title: Syntabulin regulates the trafficking of PICK1-containing vesicles in neurons.
ICA1L forms BAR-domain complexes with PICK1 and is crucial for acrosome formation in spermiogenesis.
Title: ICA1L forms BAR-domain complexes with PICK1 and is crucial for acrosome formation in spermiogenesis.
PKA and protein interacting with C kinase 1/calcineurin differentially regulate ASIC1-mediated store-operated Ca(2+) entry
Title: PICK1/calcineurin suppress ASIC1-mediated Ca2+ entry in rat pulmonary arterial smooth muscle cells.
PICK1 binds Rac1 and Cdc42, via distinct but overlapping binding sites
Title: PICK1 links AMPA receptor stimulation to Cdc42.

Submit: New GeneRIF Correction See all GeneRIFs (43)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables Arp2/3 complex binding	IDA Inferred from Direct Assay more info	PubMed
enables G protein-coupled glutamate receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables G protein-coupled glutamate receptor binding	TAS Traceable Author Statement more info	PubMed
enables G protein-coupled receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables GTPase binding	TAS Traceable Author Statement more info	PubMed
enables SNAP receptor activity	IDA Inferred from Direct Assay more info	PubMed
enables actin binding	IEA Inferred from Electronic Annotation more info
enables actin filament binding	IDA Inferred from Direct Assay more info	PubMed
enables cytoskeletal protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ephrin receptor binding	TAS Traceable Author Statement more info	PubMed
enables identical protein binding	IDA Inferred from Direct Assay more info	PubMed
enables identical protein binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables ionotropic glutamate receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables membrane curvature sensor activity	IEA Inferred from Electronic Annotation more info
enables membrane curvature sensor activity	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables phospholipid binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	IEA Inferred from Electronic Annotation more info
enables protein domain specific binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein kinase C binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein kinase C binding	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase C binding	ISO Inferred from Sequence Orthology more info
enables protein kinase C binding	ISS Inferred from Sequence or Structural Similarity more info
enables receptor tyrosine kinase binding	TAS Traceable Author Statement more info	PubMed
enables signaling receptor binding	ISO Inferred from Sequence Orthology more info
enables signaling receptor binding	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in cellular response to decreased oxygen levels	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to glucose starvation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dendritic spine maintenance	IBA Inferred from Biological aspect of Ancestor more info
involved_in dendritic spine maintenance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dendritic spine organization	IDA Inferred from Direct Assay more info	PubMed
involved_in dopamine transport	NAS Non-traceable Author Statement more info	PubMed
involved_in glial cell development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular protein transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in long-term synaptic depression	IDA Inferred from Direct Assay more info	PubMed
involved_in membrane fusion	IEA Inferred from Electronic Annotation more info
involved_in monoamine transport	IEA Inferred from Electronic Annotation more info
involved_in monoamine transport	ISO Inferred from Sequence Orthology more info
involved_in monoamine transport	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of Arp2/3 complex-mediated actin nucleation	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of receptor internalization	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of receptor internalization	IDA Inferred from Direct Assay more info	PubMed
involved_in protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within protein targeting	ISO Inferred from Sequence Orthology more info
involved_in receptor clustering	IBA Inferred from Biological aspect of Ancestor more info
involved_in receptor clustering	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in receptor clustering	ISO Inferred from Sequence Orthology more info
involved_in receptor clustering	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of Arp2/3 complex-mediated actin nucleation	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of insulin secretion	IEA Inferred from Electronic Annotation more info
involved_in regulation of insulin secretion	ISO Inferred from Sequence Orthology more info
involved_in regulation of kainate selective glutamate receptor activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of postsynaptic neurotransmitter receptor internalization	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of postsynaptic neurotransmitter receptor internalization	IEP Inferred from Expression Pattern more info	PubMed
involved_in regulation of postsynaptic neurotransmitter receptor internalization	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
located_in Golgi apparatus	ISS Inferred from Sequence or Structural Similarity more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in dendrite	IDA Inferred from Direct Assay more info	PubMed
located_in dendritic spine	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in intracellular membrane-bounded organelle	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	ISO Inferred from Sequence Orthology more info
is_active_in neuron projection	IBA Inferred from Biological aspect of Ancestor more info
located_in neuron projection	IDA Inferred from Direct Assay more info	PubMed
located_in neuron projection	IEA Inferred from Electronic Annotation more info
located_in perinuclear region of cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in plasma membrane	TAS Traceable Author Statement more info
is_active_in postsynaptic density	IBA Inferred from Biological aspect of Ancestor more info
located_in postsynaptic density	IDA Inferred from Direct Assay more info	PubMed
located_in postsynaptic density	IEA Inferred from Electronic Annotation more info
located_in postsynaptic density	ISO Inferred from Sequence Orthology more info
is_active_in postsynaptic early endosome	IBA Inferred from Biological aspect of Ancestor more info
is_active_in postsynaptic endocytic zone	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynaptic endocytic zone	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in postsynaptic specialization	IDA Inferred from Direct Assay more info	PubMed
located_in presynaptic membrane	IEA Inferred from Electronic Annotation more info
located_in presynaptic membrane	ISO Inferred from Sequence Orthology more info
located_in presynaptic membrane	ISS Inferred from Sequence or Structural Similarity more info
part_of protein-containing complex	IDA Inferred from Direct Assay more info	PubMed
located_in synapse	IDA Inferred from Direct Assay more info	PubMed
located_in synapse	ISO Inferred from Sequence Orthology more info
located_in synapse	ISS Inferred from Sequence or Structural Similarity more info
is_active_in synaptic vesicle	IBA Inferred from Biological aspect of Ancestor more info
is_active_in synaptic vesicle	IDA Inferred from Direct Assay more info	PubMed
is_active_in trans-Golgi network membrane	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: PRKCA-binding protein

Names: protein interacting with C kinase 1; protein kinase C-alpha-binding protein; protein that interacts with C kinase 1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_053460.2 → NP_445912.2 PRKCA-binding protein

Status: VALIDATED

Source sequence(s)

JAXUCZ010000007

UniProtKB/Swiss-Prot

Q546X4, Q925D1, Q9EP80

UniProtKB/TrEMBL

A6HSQ5, A6HSQ7, Q6GQQ2

Conserved Domains (2) summary

cd07659
Location:146 → 360

BAR_PICK1; The Bin/Amphiphysin/Rvs (BAR) domain of Protein Interacting with C Kinase 1

smart00228
Location:19 → 99

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086025.1 Reference GRCr8

Range

112676890..112697275

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039079986.2 → XP_038935914.1 PRKCA-binding protein isoform X1

UniProtKB/Swiss-Prot

Q546X4, Q925D1, Q9EP80

UniProtKB/TrEMBL

A6HSQ5, A6HSQ7, Q6GQQ2

Related

ENSRNOP00000016077.3, ENSRNOT00000016077.8

Conserved Domains (2) summary

cd07659
Location:146 → 360

BAR_PICK1; The Bin/Amphiphysin/Rvs (BAR) domain of Protein Interacting with C Kinase 1

smart00228
Location:19 → 99

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2
XM_063264344.1 → XP_063120414.1 PRKCA-binding protein isoform X2

UniProtKB/TrEMBL

A6HSQ8