Mthfd2l methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2-like [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Mthfd2lprovided by RGD
Official Full Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2-likeprovided by RGD
Primary source: RGD:1310879
See related: EnsemblRapid:ENSRNOG00000021347 AllianceGenome:RGD:1310879
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: RGD1310879
Summary: Enables methenyltetrahydrofolate cyclohydrolase activity; methylenetetrahydrofolate dehydrogenase (NAD+) activity; and methylenetetrahydrofolate dehydrogenase (NADP+) activity. Involved in 10-formyltetrahydrofolate metabolic process and tetrahydrofolate interconversion. Located in mitochondrial inner membrane and mitochondrial matrix. Orthologous to human MTHFD2L (methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2 like). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Thymus (RPKM 15.2), Heart (RPKM 10.5) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 14p22

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	14	NC_086032.1 (17374139..17455508, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	14	NC_051349.1 (17089947..17170036, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	14	NC_005113.4 (18640274..18721298, complement)

Chromosome 14 - NC_086032.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

We show here that the MTHFD2L enzyme is bifunctional, possessing both CH2-THF dehydrogenase and 5,10-methenyl-THF cyclohydrolase activities.
Title: Mitochondrial MTHFD2L is a dual redox cofactor-specific methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase expressed in both adult and embryonic tissues.
A new mitochondrial CH(2)-THF dehydrogenase isozyme, encoded by the MTHFD2L gene, has now been identified. The recombinant protein exhibits robust NADP(+)-dependent CH(2)-THF dehydrogenase activity when expressed in yeast.
Title: Mammalian MTHFD2L encodes a mitochondrial methylenetetrahydrofolate dehydrogenase isozyme expressed in adult tissues.

Submit: New GeneRIF Correction

General gene information

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Markers

BF390183 (e-PCR)
- UniSTS:249399

RH138232 (e-PCR)
- UniSTS:220319

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables methenyltetrahydrofolate cyclohydrolase activity	IBA Inferred from Biological aspect of Ancestor more info
enables methenyltetrahydrofolate cyclohydrolase activity	IDA Inferred from Direct Assay more info	PubMed
NOT enables methylenetetrahydrofolate dehydrogenase (NAD+) activity	IDA Inferred from Direct Assay more info	PubMed
enables methylenetetrahydrofolate dehydrogenase (NAD+) activity	IDA Inferred from Direct Assay more info	PubMed
enables methylenetetrahydrofolate dehydrogenase (NAD+) activity	IEA Inferred from Electronic Annotation more info
enables methylenetetrahydrofolate dehydrogenase (NADP+) activity	IBA Inferred from Biological aspect of Ancestor more info
enables methylenetetrahydrofolate dehydrogenase (NADP+) activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in 10-formyltetrahydrofolate metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in L-histidine biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in methionine biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in purine nucleotide biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in tetrahydrofolate interconversion	IBA Inferred from Biological aspect of Ancestor more info
involved_in tetrahydrofolate interconversion	IDA Inferred from Direct Assay more info	PubMed
involved_in tetrahydrofolate interconversion	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in mitochondrial inner membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial matrix	IDA Inferred from Direct Assay more info	PubMed
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	ISO Inferred from Sequence Orthology more info	PubMed

General protein information

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Preferred Names: bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2, mitochondrial

Names: MTHFD2-like; NADP-dependent methylenetetrahydrofolate dehydrogenase 2-like protein; methylenetetrahydrofolate dehydrogenase 2-like; probable bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2

NP_001100681.1

EC 1.5.1.15

EC 1.5.1.5

EC 3.5.4.9

XP_017454665.1

EC 1.5.1.15

EC 1.5.1.5

EC 3.5.4.9

XP_063129139.1

EC 1.5.1.15

EC 1.5.1.5

EC 3.5.4.9

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001107211.2 → NP_001100681.1 bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2, mitochondrial

See identical proteins and their annotated locations for NP_001100681.1

Status: PROVISIONAL

Source sequence(s)

JAXUCZ010000014

UniProtKB/Swiss-Prot

D3ZUA0

Related

ENSRNOP00000044342.4, ENSRNOT00000047708.7

Conserved Domains (1) summary

COG0190
Location:44 → 335

FolD; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase [Coenzyme transport and metabolism]

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086032.1 Reference GRCr8

Range

17374139..17455508 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063273069.1 → XP_063129139.1 bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2, mitochondrial isoform X1

UniProtKB/TrEMBL

A6KKD2
XM_017599176.2 → XP_017454665.1 bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2, mitochondrial isoform X2