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    Nit2 nitrilase family, member 2 [ Rattus norvegicus (Norway rat) ]

    Gene ID: 288174, updated on 9-Dec-2024

    Summary

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    Official Symbol
    Nit2provided by RGD
    Official Full Name
    nitrilase family, member 2provided by RGD
    Primary source
    RGD:1310494
    See related
    EnsemblRapid:ENSRNOG00000027797 AllianceGenome:RGD:1310494
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Rattus norvegicus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
    Also known as
    RGD1310494
    Summary
    Predicted to enable omega-amidase activity. Predicted to be involved in asparagine metabolic process; glutamine metabolic process; and oxaloacetate metabolic process. Predicted to be located in centrosome; cytosol; and mitochondrion. Orthologous to human NIT2 (nitrilase family member 2). [provided by Alliance of Genome Resources, Dec 2024]
    Expression
    Biased expression in Liver (RPKM 1128.0), Kidney (RPKM 1005.5) and 8 other tissues See more
    Orthologs
    human mouse all
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    Genomic context

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    See Nit2 in Genome Data Viewer
    Location:
    11q12
    Exon count:
    10
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCr8 (GCF_036323735.1) 11 NC_086029.1 (56832957..56844142)
    RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 11 NC_051346.1 (43363839..43378713)
    106 previous assembly Rnor_6.0 (GCF_000001895.5) 11 NC_005110.4 (45462345..45473107)

    Chromosome 11 - NC_086029.1Genomic Context describing neighboring genes Neighboring gene TBC1 domain family, member 23 Neighboring gene transfer RNA alanine (anticodon AGC) 25 Neighboring gene ribosome biogenesis protein NSA2 homolog pseudogene Neighboring gene translocase of outer mitochondrial membrane 70 Neighboring gene uncharacterized LOC108352328

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
    • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
    • BioProject: PRJNA238328
    • Publication: PMID 24510058
    • Analysis date: Mon Jun 6 17:44:12 2016

    Bibliography

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    Related articles in PubMed

    1. Identification of the putative tumor suppressor Nit2 as omega-amidase, an enzyme metabolically linked to glutamine and asparagine transamination. Krasnikov BF, et al. Biochimie, 2009 Sep. PMID 19595734, Free PMC Article
    2. Structural insights into the catalytic active site and activity of human Nit2/ω-amidase: kinetic assay and molecular dynamics simulation. Chien CH, et al. J Biol Chem, 2012 Jul 27. PMID 22674578, Free PMC Article
    3. Interacting Network of the Gap Junction (GJ) Protein Connexin43 (Cx43) is Modulated by Ischemia and Reperfusion in the Heart. Martins-Marques T, et al. Mol Cell Proteomics, 2015 Nov. PMID 26316108, Free PMC Article
    4. Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria. Mootha VK, et al. Cell, 2003 Nov 26. PMID 14651853
    5. Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context. Morgenstern M, et al. Cell Metab, 2021 Dec 7. PMID 34800366, Free PMC Article

    See all (9) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Data report that rat liver Nit2 is omega-amidase and in vivo amidase substrates are alpha-ketoglutaramate and alpha-ketosuccinamate, generated by transamination of glutamine and asparagine, respectively.
      Title: Identification of the putative tumor suppressor Nit2 as omega-amidase, an enzyme metabolically linked to glutamine and asparagine transamination.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Clone Names

    • MGC124762

    Gene Ontology Provided by RGD

    Function Evidence Code Pubs
    enables 2-oxoglutaramate amidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables omega-amidase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables omega-amidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables omega-amidase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    involved_in asparagine metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in asparagine metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in asparagine metabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in glutamine metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in glutamine metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in glutamine metabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in oxaloacetate metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in oxaloacetate metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in oxaloacetate metabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    Component Evidence Code Pubs
    located_in centrosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in centrosome ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in mitochondrion ISO
    Inferred from Sequence Orthology
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    omega-amidase NIT2
    Names
    Nit protein 2
    nitrilase homolog 2
    NP_001029298.1
    XP_038944121.1
    XP_038944122.1
    XP_063126479.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001034126.1 → NP_001029298.1  omega-amidase NIT2

      See identical proteins and their annotated locations for NP_001029298.1

      Status: PROVISIONAL

      Source sequence(s)
      BC100637
      UniProtKB/Swiss-Prot
      Q497B0
      UniProtKB/TrEMBL
      A0A8I5ZM02, A6IQN1
      Related
      ENSRNOP00000078811.1, ENSRNOT00000105850.2
      Conserved Domains (1) summary
      cd07572
      Location:5 → 265
      nit; Nit1, Nit 2, and related proteins, and the Nit1-like domain of NitFhit (class 10 nitrilases)

    RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCr8

    Genomic

    1. NC_086029.1 Reference GRCr8

      Range
      56832957..56844142
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_039088193.1 → XP_038944121.1  omega-amidase NIT2 isoform X1

      UniProtKB/TrEMBL
      A0A8L2QJE6
      Related
      ENSRNOP00000034144.5, ENSRNOT00000029420.6
      Conserved Domains (1) summary
      cd07572
      Location:89 → 349
      nit; Nit1, Nit 2, and related proteins, and the Nit1-like domain of NitFhit (class 10 nitrilases)

    2. XM_039088194.2 → XP_038944122.1  omega-amidase NIT2 isoform X2

      UniProtKB/TrEMBL
      A0A8L2QJE6
      Conserved Domains (1) summary
      cd07572
      Location:89 → 316
      nit; Nit1, Nit 2, and related proteins, and the Nit1-like domain of NitFhit (class 10 nitrilases)

    3. XM_063270409.1 → XP_063126479.1  omega-amidase NIT2 isoform X3

      UniProtKB/TrEMBL
      A0A8I5ZM02
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q497B0.1 GenPept UniProtKB/Swiss-Prot:Q497B0

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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