Chek2 checkpoint kinase 2 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Chek2provided by RGD
Official Full Name: checkpoint kinase 2provided by RGD
Primary source: RGD:621543
See related: EnsemblRapid:ENSRNOG00000037509 AllianceGenome:RGD:621543
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Chk2; Rad53
Summary: Enables protein serine/threonine kinase activity. Involved in several processes, including cellular response to bisphenol A; positive regulation of anoikis; and regulation of cell cycle phase transition. Predicted to be located in Golgi apparatus; PML body; and chromosome, telomeric region. Predicted to be active in cytoplasm and nucleus. Biomarker of urinary bladder cancer. Human ortholog(s) of this gene implicated in several diseases, including Li-Fraumeni syndrome 2; breast cancer (multiple); colorectal cancer; osteosarcoma; and prostate cancer. Orthologous to human CHEK2 (checkpoint kinase 2). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Thymus (RPKM 60.6), Spleen (RPKM 55.8) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 12q16

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	12	NC_086030.1 (51448838..51481159, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	12	NC_051347.1 (45788823..45821382, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	12	NC_005111.4 (51845574..51878098, complement)

Chromosome 12 - NC_086030.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Chk-2 is an important novel component of anoikis-promoting machinery of intestinal epithelial cells
Title: Tumor suppressor protein kinase Chk2 is a mediator of anoikis of intestinal epithelial cells.
Data demonstrate that polyamines regulate c-Myc translation in IECs through HuR phosphorylation by Chk2 and provide new insight into the molecular functions of cellular polyamines.
Title: Polyamines regulate c-Myc translation through Chk2-dependent HuR phosphorylation.
an important role for the DNA damage response mediated by ATR-Chk2 in p53 activation and renal cell apoptosis during cisplatin nephrotoxicity.
Title: ATR-Chk2 signaling in p53 activation and DNA damage response during cisplatin-induced apoptosis.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH130548 (e-PCR)
- UniSTS:213832

RH141098 (e-PCR)
- UniSTS:223095

AU049427 (e-PCR)
- UniSTS:145874

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase activity	ISO Inferred from Sequence Orthology more info
enables protein kinase binding	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in DNA damage checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within DNA damage response	ISO Inferred from Sequence Orthology more info
involved_in DNA damage response	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator	ISO Inferred from Sequence Orthology more info
involved_in G2/M transition of mitotic cell cycle	ISO Inferred from Sequence Orthology more info
involved_in cellular response to bisphenol A	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within cellular response to gamma radiation	ISO Inferred from Sequence Orthology more info
involved_in cellular response to stress	ISO Inferred from Sequence Orthology more info
involved_in cellular response to xenobiotic stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in double-strand break repair	ISO Inferred from Sequence Orthology more info
involved_in intrinsic apoptotic signaling pathway in response to DNA damage	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator	ISO Inferred from Sequence Orthology more info
involved_in mitotic intra-S DNA damage checkpoint signaling	ISO Inferred from Sequence Orthology more info
involved_in mitotic spindle assembly	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of DNA damage checkpoint	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in peptidyl-serine phosphorylation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within peptidyl-serine phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of anoikis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of protein phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein autophosphorylation	ISO Inferred from Sequence Orthology more info
involved_in protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in protein stabilization	ISO Inferred from Sequence Orthology more info
involved_in regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in regulation of autophagosome assembly	ISO Inferred from Sequence Orthology more info
involved_in regulation of protein catabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within response to gamma radiation	ISO Inferred from Sequence Orthology more info
involved_in response to glycoside	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within signal transduction in response to DNA damage	ISO Inferred from Sequence Orthology more info
involved_in signal transduction in response to DNA damage	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within thymocyte apoptotic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
located_in PML body	ISO Inferred from Sequence Orthology more info
located_in chromosome, telomeric region	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: serine/threonine-protein kinase Chk2

Names: CHK2 checkpoint homolog; protein kinase Chk2

NP_446129.1

EC 2.7.11.1

XP_006249580.1

EC 2.7.11.1

XP_006249581.1

EC 2.7.11.1

XP_006249582.1

EC 2.7.11.1

XP_006249583.1

EC 2.7.11.1

XP_063127056.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_053677.1 → NP_446129.1 serine/threonine-protein kinase Chk2

See identical proteins and their annotated locations for NP_446129.1

Status: PROVISIONAL

Source sequence(s)

AF134054

UniProtKB/TrEMBL

A0A0G2K8L3, A6J285, F7F4E2, Q9R019

Related

ENSRNOP00000053643.3, ENSRNOT00000056800.3

Conserved Domains (2) summary

cd00060
Location:96 → 204

FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...

cd14084
Location:216 → 489

STKc_Chk2; Catalytic domain of the Serine/Threonine kinase, Cell cycle Checkpoint Kinase 2

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086030.1 Reference GRCr8

Range

51448838..51481159 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006249519.4 → XP_006249581.1 serine/threonine-protein kinase Chk2 isoform X1

See identical proteins and their annotated locations for XP_006249581.1

UniProtKB/TrEMBL

A0A0G2K8L3, A6J285, F7F4E2, Q9R019

Conserved Domains (2) summary

cd00060
Location:96 → 204

FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...

cd14084
Location:216 → 489

STKc_Chk2; Catalytic domain of the Serine/Threonine kinase, Cell cycle Checkpoint Kinase 2
XM_006249521.5 → XP_006249583.1 serine/threonine-protein kinase Chk2 isoform X1

See identical proteins and their annotated locations for XP_006249583.1

UniProtKB/TrEMBL

A0A0G2K8L3, A6J285, F7F4E2, Q9R019

Conserved Domains (2) summary

cd00060
Location:96 → 204

FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...

cd14084
Location:216 → 489

STKc_Chk2; Catalytic domain of the Serine/Threonine kinase, Cell cycle Checkpoint Kinase 2
XM_006249518.5 → XP_006249580.1 serine/threonine-protein kinase Chk2 isoform X1

See identical proteins and their annotated locations for XP_006249580.1

UniProtKB/TrEMBL

A0A0G2K8L3, A6J285, F7F4E2, Q9R019

Conserved Domains (2) summary

cd00060
Location:96 → 204

FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...

cd14084
Location:216 → 489

STKc_Chk2; Catalytic domain of the Serine/Threonine kinase, Cell cycle Checkpoint Kinase 2
XM_006249520.5 → XP_006249582.1 serine/threonine-protein kinase Chk2 isoform X1

See identical proteins and their annotated locations for XP_006249582.1

UniProtKB/TrEMBL

A0A0G2K8L3, A6J285, F7F4E2, Q9R019

Conserved Domains (2) summary

cd00060
Location:96 → 204

FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...

cd14084
Location:216 → 489

STKc_Chk2; Catalytic domain of the Serine/Threonine kinase, Cell cycle Checkpoint Kinase 2
XM_063270986.1 → XP_063127056.1 serine/threonine-protein kinase Chk2 isoform X2

UniProtKB/TrEMBL

F7F4E2

Related

ENSRNOP00000109711.1, ENSRNOT00000163168.1