MCM3 minichromosome maintenance complex component 3 [Homo sapiens (human)] - Gene

Summary

Official Symbol: MCM3provided by HGNC
Official Full Name: minichromosome maintenance complex component 3provided by HGNC
Primary source: HGNC:HGNC:6945
See related: Ensembl:ENSG00000112118 MIM:602693; AllianceGenome:HGNC:6945
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: HCC5; P1.h; RLFB; P1-MCM3
Summary: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2018]
Expression: Ubiquitous expression in lymph node (RPKM 33.0), appendix (RPKM 23.5) and 24 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 6p12.2

Exon count:: 20

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	6	NC_000006.12 (52264015..52284742, complement)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	6	NC_060930.1 (52102764..52123487, complement)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	6	NC_000006.11 (52128813..52149540, complement)

Chromosome 6 - NC_000006.12 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Tissue-specific circular RNA induction during human fetal development
Description: 35 human fetal samples from 6 tissues (3 - 7 replicates per tissue) collected between 10 and 20 weeks gestational time were sequenced using Illumina TruSeq Stranded Total RNA
BioProject: PRJNA270632
Publication: PMID 26076956
Analysis date: Mon Apr 2 22:54:59 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

MCM3 is a novel proliferation marker associated with longer survival for patients with tubo-ovarian high-grade serous carcinoma.
Title: MCM3 is a novel proliferation marker associated with longer survival for patients with tubo-ovarian high-grade serous carcinoma.
MCM complex members MCM3 and MCM7 are associated with a phenotypic spectrum from Meier-Gorlin syndrome to lipodystrophy and adrenal insufficiency.
Title: MCM complex members MCM3 and MCM7 are associated with a phenotypic spectrum from Meier-Gorlin syndrome to lipodystrophy and adrenal insufficiency.
Single-cell chromatin accessibility landscape of human umbilical cord blood in trisomy 18 syndrome.
Title: Single-cell chromatin accessibility landscape of human umbilical cord blood in trisomy 18 syndrome.
Bioinformatics Analysis Reveals MCM3 as an Important Prognostic Marker in Cervical Cancer.
Title: Bioinformatics Analysis Reveals MCM3 as an Important Prognostic Marker in Cervical Cancer.
The Immunohistochemical Expression of MCM-3, -5, and -7 Proteins in the Uterine Fibroids.
Title: The Immunohistochemical Expression of MCM-3, -5, and -7 Proteins in the Uterine Fibroids.
PLK1 promotes proliferation and suppresses apoptosis of renal cell carcinoma cells by phosphorylating MCM3.
Title: PLK1 promotes proliferation and suppresses apoptosis of renal cell carcinoma cells by phosphorylating MCM3.
MCM3 proliferative index is worthier over Ki-67 in the characterization of salivary gland tumors.
Title: MCM3 proliferative index is worthier over Ki-67 in the characterization of salivary gland tumors.
Elevated expression of minichromosome maintenance 3 indicates poor outcomes and promotes G1/S cell cycle progression, proliferation, migration and invasion in colorectal cancer.
Title: Elevated expression of minichromosome maintenance 3 indicates poor outcomes and promotes G1/S cell cycle progression, proliferation, migration and invasion in colorectal cancer.
Gene expression profiling revealed MCM3 to be a better marker than Ki67 in prognosis of invasive ductal breast carcinoma patients.
Title: Gene expression profiling revealed MCM3 to be a better marker than Ki67 in prognosis of invasive ductal breast carcinoma patients.
Results show that found DNA replication initiation protein MCM3 was upregulated in hepatocellular carcinoma (HCC) tissues and cells and its expression correlated with poor outcome and radioresistance. MCM3 promoted radioresistance through activating NF-kappaB pathway, strengthening the role of MCM subunits in the tumor progression. These Findings revealed an independent prognostic factor of MCM3 for HCC.
Title: Minichromosome maintenance 3 promotes hepatocellular carcinoma radioresistance by activating the NF-κB pathway.

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Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Protein interactions

Protein	Gene	Interaction	Pubs
Rev	rev	HIV-1 Rev co-localizes with minichromosome maintenance complex component 3 (MCM3) in the cytoplasmic and nucleoplasmic compartments of cells	PubMed
Tat	tat	HIV-1 Tat upregulates the expression of minichromosome maintenance complex component 3 (MCM3) in Jurkat cells	PubMed
	tat	Interaction of HIV-1 Tat with MCM3 in T-cells is identified by a proteomic strategy based on affinity chromatography coupled with mass spectrometry	PubMed
Vpr	vpr	HIV-1 Vpr downregulates MCM3 in HeLa cells within 12 hours of exposure	PubMed

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH46848 (e-PCR)
- UniSTS:50732

csnpmcm3-pcr1-1 (e-PCR)
- UniSTS:243042

RH70755 (e-PCR)
- UniSTS:79477

RH80718 (e-PCR)
- UniSTS:89536

STS-T91828 (e-PCR)
- UniSTS:53190

RH80758 (e-PCR)
- UniSTS:87758

MARC_3939-3940:996679197:3 (e-PCR)
- UniSTS:269290

MARC_13500-13501:1002732289:3 (e-PCR)
- UniSTS:267506

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H2AZ histone chaperone activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H3-H4 histone complex chaperone activity	IEA Inferred from Electronic Annotation more info
enables DNA binding	TAS Traceable Author Statement more info	PubMed
enables DNA clamp loader activity	IEA Inferred from Electronic Annotation more info
enables chromatin extrusion motor activity	IEA Inferred from Electronic Annotation more info
enables cohesin loader activity	IEA Inferred from Electronic Annotation more info
enables double-stranded DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables forked DNA-dependent helicase activity	IEA Inferred from Electronic Annotation more info
enables four-way junction helicase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables single-stranded 3'-5' DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables single-stranded DNA binding	IBA Inferred from Biological aspect of Ancestor more info
contributes_to single-stranded DNA helicase activity	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in DNA replication initiation	TAS Traceable Author Statement more info	PubMed
involved_in DNA strand elongation involved in DNA replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA unwinding involved in DNA replication	IDA Inferred from Direct Assay more info	PubMed
involved_in chromatin looping	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair via break-induced replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic DNA replication initiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of DNA-templated DNA replication initiation	NAS Non-traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
part_of CMG complex	IPI Inferred from Physical Interaction more info	PubMed
part_of CMG complex	ISS Inferred from Sequence or Structural Similarity more info
part_of MCM complex	IBA Inferred from Biological aspect of Ancestor more info
part_of MCM complex	IDA Inferred from Direct Assay more info	PubMed
part_of MCM complex	IPI Inferred from Physical Interaction more info	PubMed
part_of alpha DNA polymerase:primase complex	TAS Traceable Author Statement more info	PubMed
located_in centrosome	IDA Inferred from Direct Assay more info
located_in chromosome, telomeric region	HDA more info	PubMed
located_in membrane	HDA more info	PubMed
NOT located_in nucleolus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	IDA Inferred from Direct Assay more info
located_in nucleoplasm	TAS Traceable Author Statement more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear region of cytoplasm	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: DNA replication licensing factor MCM3

Names: DNA polymerase alpha holoenzyme-associated protein P1; DNA replication factor MCM3; MCM3 minichromosome maintenance deficient 3; RLF subunit beta; cervical cancer proto-oncogene 5; hRlf beta subunit; minichromosome maintenance deficient 3; p102; replication licensing factor, beta subunit

NP_001257401.2

EC 3.6.4.12

NP_001353298.1

EC 3.6.4.12

NP_001353299.1

EC 3.6.4.12

NP_001353300.1

EC 3.6.4.12

NP_001353301.1

EC 3.6.4.12

NP_001353302.1

EC 3.6.4.12

NP_001353303.1

EC 3.6.4.12

NP_001353304.1

EC 3.6.4.12

NP_002379.4

EC 3.6.4.12

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001270472.3 → NP_001257401.2 DNA replication licensing factor MCM3 isoform 2

Status: REVIEWED

Source sequence(s)

AL034343

Consensus CDS

CCDS75468.2

UniProtKB/TrEMBL

B4DUQ9, J3KQ69

Related

ENSP00000229854.6, ENST00000229854.12

Conserved Domains (1) summary

smart00350
Location:63 → 608

MCM; minichromosome maintenance proteins
NM_001366369.2 → NP_001353298.1 DNA replication licensing factor MCM3 isoform 3

Status: REVIEWED

Source sequence(s)

AL034343

UniProtKB/TrEMBL

A0A0S2Z492, Q53HJ4

Conserved Domains (2) summary

smart00350
Location:109 → 654

MCM; minichromosome maintenance proteins

pfam14551
Location:19 → 104

MCM_N; MCM N-terminal domain
NM_001366370.2 → NP_001353299.1 DNA replication licensing factor MCM3 isoform 4

Status: REVIEWED

Source sequence(s)

AL034343

UniProtKB/TrEMBL

Q53HJ4

Conserved Domains (2) summary

smart00350
Location:109 → 671

MCM; minichromosome maintenance proteins

pfam14551
Location:19 → 104

MCM_N; MCM N-terminal domain
NM_001366371.2 → NP_001353300.1 DNA replication licensing factor MCM3 isoform 5

Status: REVIEWED

Source sequence(s)

AL034343

UniProtKB/TrEMBL

Q53HJ4

Conserved Domains (2) summary

smart00350
Location:109 → 637

MCM; minichromosome maintenance proteins

pfam14551
Location:19 → 104

MCM_N; MCM N-terminal domain
NM_001366372.2 → NP_001353301.1 DNA replication licensing factor MCM3 isoform 6

Status: REVIEWED

Source sequence(s)

AL034343

UniProtKB/TrEMBL

Q53HJ4

Conserved Domains (2) summary

smart00350
Location:109 → 637

MCM; minichromosome maintenance proteins

pfam14551
Location:19 → 104

MCM_N; MCM N-terminal domain
NM_001366373.2 → NP_001353302.1 DNA replication licensing factor MCM3 isoform 7

Status: REVIEWED

Source sequence(s)

AL034343

UniProtKB/TrEMBL

B4DS46

Conserved Domains (1) summary

cl25443
Location:6 → 481

MCM_OB; MCM OB domain
NM_001366374.2 → NP_001353303.1 DNA replication licensing factor MCM3 isoform 8

Status: REVIEWED

Source sequence(s)

AL034343

UniProtKB/TrEMBL

B4DS46

Conserved Domains (1) summary

cl25443
Location:1 → 451

MCM_OB; MCM OB domain
NM_001366375.2 → NP_001353304.1 DNA replication licensing factor MCM3 isoform 9

Status: REVIEWED

Source sequence(s)

AL034343

Conserved Domains (2) summary

COG1241
Location:17 → 165

Mcm2; DNA replicative helicase MCM subunit Mcm2, Cdc46/Mcm family [Replication, recombination and repair]

cl25443
Location:177 → 373

MCM_OB; MCM OB domain
NM_002388.6 → NP_002379.4 DNA replication licensing factor MCM3 isoform 1

Status: REVIEWED

Source sequence(s)

AL034343

Consensus CDS

CCDS4940.3

UniProtKB/Swiss-Prot

B4DWW4, P25205, Q92660, Q9BTR3, Q9NUE7

UniProtKB/TrEMBL

A0A0S2Z4T1, Q53HJ4

Related

ENSP00000472940.2, ENST00000596288.7

Conserved Domains (2) summary

smart00350
Location:109 → 654

MCM; minichromosome maintenance proteins

pfam14551
Location:16 → 102

MCM_N; MCM N-terminal domain