Slc9a3 solute carrier family 9 member A3 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Slc9a3provided by RGD
Official Full Name: solute carrier family 9 member A3provided by RGD
Primary source: RGD:3720
See related: EnsemblRapid:ENSRNOG00000015159 AllianceGenome:RGD:3720
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Nhe3
Summary: Enables sodium:proton antiporter activity. Involved in several processes, including regulation of pH; response to glucocorticoid; and sodium ion transport. Located in apical plasma membrane; brush border membrane; and recycling endosome membrane. Used to study hypertension. Biomarker of obesity and renovascular hypertension. Human ortholog(s) of this gene implicated in congenital secretory sodium diarrhea 8. Orthologous to human SLC9A3 (solute carrier family 9 member A3). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Kidney (RPKM 198.2) and Brain (RPKM 11.8) See more
Orthologs: human mouse all
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Genomic context

Location:: 1p11

Exon count:: 17

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	1	NC_086019.1 (30953215..30996209, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	1	NC_051336.1 (29124633..29167912, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	1	NC_005100.4 (31731652..31777144, complement)

Chromosome 1 - NC_086019.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

mOrange2, a Genetically Encoded, pH Sensitive Fluorescent Protein, is an Alternative to BCECF-AM to Measure Intracellular pH to Determine NHE3 and DRA Activity.
Title: mOrange2, a Genetically Encoded, pH Sensitive Fluorescent Protein, is an Alternative to BCECF-AM to Measure Intracellular pH to Determine NHE3 and DRA Activity.
Computational model of the renal circadian clock predicting effect of circadian clock modulation of NHE3 on proximal tubule cell on sodium transport.
Title: Predicted effect of circadian clock modulation of NHE3 of a proximal tubule cell on sodium transport.
In spontaneously hypertensive rats, a high plasma level of ANG-(1-7) inhibited NHE3 in the proximal tubule, which mitigated the hypertension caused by the high plasma level of ANG II.
Title: The effects of angiotensin-(1-7) on the exchanger NHE3 and on [Ca(2+)](i) in the proximal tubules of spontaneously hypertensive rats.
Results show that renal afferents leads to activation of catecholaminergic and non-catecholaminergic neurons in the medulla oblongata. When renorenal reflex is induced, NHE3 exchanger activity appears to be decreased, resulting in decreased sodium and water reabsorption in the contralateral kidney.
Title: Stimulation of renal afferent fibers leads to activation of catecholaminergic and non-catecholaminergic neurons in the medulla oblongata.
Reduction of intestinal sodium absorption by selective NHE3 inhibition in the gut lowered high blood pressure and reduced LV remodeling without deteriorating renal functional and structural parameters in obese spontaneously hypertensive rats.
Title: Inhibition of NHE3-mediated Sodium Absorption in the Gut Reduced Cardiac End-organ Damage Without Deteriorating Renal Function in Obese Spontaneously Hypertensive Rats.
Increased pendrin in pregnant rats may compensate for the decreased NCC and aid in the renal sodium chloride reabsorption of pregnancy.
Title: The chloride-bicarbonate exchanger pendrin is increased in the kidney of the pregnant rat.
a potential link between NHE3 and Na(+) /K(+) -ATPase in the pathophysiological changes in renal protein handling observed in obesity
Title: Diet induced obesity in rats reduces NHE3 and Na(+) /K(+) -ATPase expression in the kidney.
These findings indicate that biased signaling of the beta-arrestin pathway through the AT1 receptor inhibits NHE3 activity in the proximal tubule at least in part due to changes in NHE3 subcellular localization.
Title: Proximal tubule NHE3 activity is inhibited by beta-arrestin-biased angiotensin II type 1 receptor signaling.
In hypertensive rats, the plasma and tissue renin angiotensin systems are activated, increased Ang II stimulates the expression of NHE3 and SGK1.
Title: Novel mechanism of intra‑renal angiotensin II-induced sodium/proton exchanger 3 expression by losartan in spontaneously hypertensive rats.
NHE3-mediated proximal tubular sodium reabsorption induced by renal nerve stimulation occurs via intrarenal renin angiotensin system activation and triggering of the AT1 receptor/inhibitory G-protein signaling pathway.
Title: Renal nerve stimulation leads to the activation of the Na+/H+ exchanger isoform 3 via angiotensin II type I receptor.

Submit: New GeneRIF Correction See all GeneRIFs (70)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Slc9a3 (e-PCR), detects polymorphism
- UniSTS:144436

RH94714 (e-PCR)
- UniSTS:119961

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables PDZ domain binding	ISO Inferred from Sequence Orthology more info
enables PDZ domain binding	ISS Inferred from Sequence or Structural Similarity more info
enables identical protein binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISS Inferred from Sequence or Structural Similarity more info
enables phosphatidylinositol binding	IEA Inferred from Electronic Annotation more info
enables phosphatidylinositol binding	ISO Inferred from Sequence Orthology more info
enables phosphatidylinositol binding	ISS Inferred from Sequence or Structural Similarity more info
enables potassium:proton antiporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables sodium:proton antiporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables sodium:proton antiporter activity	IDA Inferred from Direct Assay more info	PubMed
enables sodium:proton antiporter activity	IEA Inferred from Electronic Annotation more info
enables sodium:proton antiporter activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in circadian rhythm	IEP Inferred from Expression Pattern more info	PubMed
involved_in potassium ion transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in proton transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in receptor-mediated endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of intracellular pH	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of intracellular pH	IEA Inferred from Electronic Annotation more info
involved_in regulation of intracellular pH	ISO Inferred from Sequence Orthology more info
involved_in regulation of pH	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of pH	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of pH	ISO Inferred from Sequence Orthology more info
involved_in regulation of sodium ion transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to glucocorticoid	IEP Inferred from Expression Pattern more info	PubMed
involved_in sodium ion import across plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
involved_in sodium ion import across plasma membrane	IEA Inferred from Electronic Annotation more info
involved_in sodium ion import across plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in sodium ion import across plasma membrane	ISS Inferred from Sequence or Structural Similarity more info
involved_in sodium ion transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in sodium ion transport	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within sodium ion transport	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
is_active_in apical plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in apical plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in apical plasma membrane	IEA Inferred from Electronic Annotation more info
located_in apical plasma membrane	ISO Inferred from Sequence Orthology more info
located_in brush border	IDA Inferred from Direct Assay more info	PubMed
is_active_in brush border membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in brush border membrane	IDA Inferred from Direct Assay more info	PubMed
located_in brush border membrane	ISO Inferred from Sequence Orthology more info
located_in early endosome	ISO Inferred from Sequence Orthology more info
located_in early endosome membrane	IEA Inferred from Electronic Annotation more info
located_in extracellular exosome	IEA Inferred from Electronic Annotation more info
located_in extracellular exosome	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in recycling endosome membrane	IDA Inferred from Direct Assay more info	PubMed
located_in recycling endosome membrane	IEA Inferred from Electronic Annotation more info
located_in recycling endosome membrane	ISO Inferred from Sequence Orthology more info
located_in vesicle	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: sodium/hydrogen exchanger 3

Names: NHE-3; Na(+)/H(+) exchanger 3; Solute carrier family 9 (sodium/hydrogen exchanger 3), antiporter 3, Na+/H+ (amiloride insensitive); plasma membrane ion transport protein; solute carrier family 9 (sodium/hydrogen exchanger), member 3; solute carrier family 9 member 3; solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001414040.1 → NP_001400969.1 sodium/hydrogen exchanger 3 precursor

Status: VALIDATED

Description

Transcript Variant: Variants 1 and 2 encode the same protein.

Source sequence(s)

JAXUCZ010000001

UniProtKB/Swiss-Prot

P26433

UniProtKB/TrEMBL

A6JUV3, G3V7Y7

Related

ENSRNOP00000083053.2, ENSRNOT00000094251.2
NM_012654.3 → NP_036786.2 sodium/hydrogen exchanger 3 precursor

Status: VALIDATED

Description

Transcript Variant: Variants 1 and 2 encode the same protein.

Source sequence(s)

JAXUCZ010000001

UniProtKB/Swiss-Prot

P26433

UniProtKB/TrEMBL

A6JUV3, G3V7Y7

Related

ENSRNOP00000020711.2, ENSRNOT00000020711.5

Conserved Domains (1) summary

TIGR00840
Location:45 → 644

b_cpa1; sodium/hydrogen exchanger 3

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086019.1 Reference GRCr8

Range

30953215..30996209 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039100846.2 → XP_038956774.1 sodium/hydrogen exchanger 3 isoform X1

UniProtKB/TrEMBL

A0A8I5ZZ59

Conserved Domains (1) summary

TIGR00840
Location:45 → 644

b_cpa1; sodium/hydrogen exchanger 3