Dgki diacylglycerol kinase, iota [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Dgkiprovided by RGD
Official Full Name: diacylglycerol kinase, iotaprovided by RGD
Primary source: RGD:735049
See related: EnsemblRapid:ENSRNOG00000026705 AllianceGenome:RGD:735049
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: rDGKi-3
Summary: Enables ATP-dependent diacylglycerol kinase activity. Involved in diacylglycerol metabolic process; lipid phosphorylation; and phosphatidic acid biosynthetic process. Located in several cellular components, including postsynaptic density; presynaptic active zone; and synaptic membrane. Is active in glutamatergic synapse and synaptic vesicle. Is extrinsic component of postsynaptic density membrane and extrinsic component of presynaptic active zone membrane. Orthologous to human DGKI (diacylglycerol kinase iota). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Brain (RPKM 114.6), Uterus (RPKM 32.4) and 3 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 4q22

Exon count:: 35

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	4	NC_086022.1 (66377897..66839790, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	4	NC_051339.1 (65410878..65872733, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	4	NC_005103.4 (64365115..64831473, complement)

Chromosome 4 - NC_086022.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Diacylglycerol kinase iota is exclusively found in the brain and is expressed in a distinct regional pattern. Differential expression of splice variants in the cortex of alcohol accepting (AA) and alcohol non-accepting (ANA) rat lines.
Title: Differential expression of diacylglycerol kinase iota and L18A mRNAs in the brains of alcohol-preferring AA and alcohol-avoiding ANA rats.
novel splice variants, which contain either insertion 1 (71 bp) or insertion 2 (19 bp) or both in the carboxyl-terminal portion
Title: Cloning and characterization of diacylglycerol kinase iota splice variants in rat brain.

Submit: New GeneRIF Correction

General gene information

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Markers

RH144498 (e-PCR)
- UniSTS:233743

AU048384 (e-PCR)
- UniSTS:109588

D4Rat135 (e-PCR), detects polymorphism
- UniSTS:118811

BE102640 (e-PCR)
- UniSTS:244293

D4Rat118 (e-PCR), detects polymorphism
- UniSTS:227709

D4Got50 (e-PCR)
- UniSTS:115090

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP-dependent diacylglycerol kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ATP-dependent diacylglycerol kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP-dependent diacylglycerol kinase activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent diacylglycerol kinase activity	ISO Inferred from Sequence Orthology more info
enables GTPase inhibitor activity	IEA Inferred from Electronic Annotation more info
enables GTPase inhibitor activity	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables small GTPase binding	IEA Inferred from Electronic Annotation more info
enables small GTPase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in Ras protein signal transduction	IEA Inferred from Electronic Annotation more info
acts_upstream_of Ras protein signal transduction	ISO Inferred from Sequence Orthology more info
involved_in biological_process	ND No biological Data available more info
involved_in diacylglycerol metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in diacylglycerol metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in excitatory postsynaptic potential	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within excitatory postsynaptic potential	ISO Inferred from Sequence Orthology more info
involved_in glycerolipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in habituation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within habituation	ISO Inferred from Sequence Orthology more info
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in lipid phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in lipid phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in neurotransmitter secretion	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within neurotransmitter secretion	ISO Inferred from Sequence Orthology more info
involved_in phosphatidic acid biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in phosphatidic acid biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in phospholipase C-activating G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of Ras protein signal transduction	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of Ras protein signal transduction	ISO Inferred from Sequence Orthology more info
involved_in presynaptic modulation of chemical synaptic transmission	IEA Inferred from Electronic Annotation more info
involved_in presynaptic modulation of chemical synaptic transmission	ISO Inferred from Sequence Orthology more info
involved_in regulation of long-term synaptic depression	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of long-term synaptic depression	ISO Inferred from Sequence Orthology more info
involved_in regulation of synaptic transmission, glutamatergic	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of synaptic transmission, glutamatergic	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in Schaffer collateral - CA1 synapse	IEA Inferred from Electronic Annotation more info
is_active_in Schaffer collateral - CA1 synapse	ISO Inferred from Sequence Orthology more info
located_in axon terminus	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in dendritic spine	ISO Inferred from Sequence Orthology more info
located_in excitatory synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in extrinsic component of postsynaptic density membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in extrinsic component of presynaptic active zone membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IBA Inferred from Biological aspect of Ancestor more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
located_in glutamatergic synapse	IEA Inferred from Electronic Annotation more info
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
part_of guanyl-nucleotide exchange factor complex	IEA Inferred from Electronic Annotation more info
part_of guanyl-nucleotide exchange factor complex	ISO Inferred from Sequence Orthology more info
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in postsynapse	IEA Inferred from Electronic Annotation more info
located_in postsynaptic density	IDA Inferred from Direct Assay more info	PubMed
located_in presynapse	IEA Inferred from Electronic Annotation more info
located_in presynaptic active zone	IDA Inferred from Direct Assay more info	PubMed
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info
located_in synapse	ISO Inferred from Sequence Orthology more info
located_in synaptic membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in synaptic vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in synaptic vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in synaptic vesicle membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: diacylglycerol kinase iota

Names: DAG kinase iota; DGK-iota; diacylglycerol kinase iota-3; diglyceride kinase iota

NP_942077.2

EC 2.7.1.107

XP_006236395.1

EC 2.7.1.107

XP_017448383.1

EC 2.7.1.107

XP_038964295.1

EC 2.7.1.107

XP_038964296.1

EC 2.7.1.107

XP_038964297.1

EC 2.7.1.107

XP_038964298.1

EC 2.7.1.107

XP_038964299.1

EC 2.7.1.107

XP_038964300.1

EC 2.7.1.107

XP_038964302.1

EC 2.7.1.107

XP_063142779.1

EC 2.7.1.107

XP_063142780.1

EC 2.7.1.107

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_198782.2 → NP_942077.2 diacylglycerol kinase iota

Status: VALIDATED

Source sequence(s)

AB058962, AW526027, CN544764, JAXUCZ010000004

UniProtKB/Swiss-Prot

F1MAB7, Q810C3, Q810C4, Q810C5

UniProtKB/TrEMBL

A0A8I6GM87

Related

ENSRNOP00000034571.4, ENSRNOT00000033268.6

Conserved Domains (7) summary

smart00045
Location:521 → 678

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:373 → 494

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

COG0666
Location:848 → 1047

ANKYR; Ankyrin repeat [Signal transduction mechanisms]

sd00045
Location:945 → 976

ANK; ANK repeat [structural motif]

pfam12796
Location:915 → 1010

Ank_2; Ankyrin repeats (3 copies)

cd20850
Location:163 → 235

C1_DGKiota_rpt1; first protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase iota (DAG kinase iota) and similar proteins

cd20896
Location:244 → 318

C1_DGKiota_rpt2; second protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase iota (DAG kinase iota) and similar proteins

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086022.1 Reference GRCr8

Range

66377897..66839790 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039108368.2 → XP_038964296.1 diacylglycerol kinase iota isoform X2

UniProtKB/TrEMBL

A0A8I6GM87

Conserved Domains (7) summary

smart00045
Location:561 → 718

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:413 → 534

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

COG0666
Location:909 → 1108

ANKYR; Ankyrin repeat [Signal transduction mechanisms]

sd00045
Location:1006 → 1037

ANK; ANK repeat [structural motif]

pfam12796
Location:976 → 1071

Ank_2; Ankyrin repeats (3 copies)

cd20850
Location:163 → 235

C1_DGKiota_rpt1; first protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase iota (DAG kinase iota) and similar proteins

cd20896
Location:244 → 318

C1_DGKiota_rpt2; second protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase iota (DAG kinase iota) and similar proteins
XM_039108367.2 → XP_038964295.1 diacylglycerol kinase iota isoform X1

UniProtKB/TrEMBL

A0A8I6GM87

Conserved Domains (7) summary

smart00045
Location:562 → 719

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:414 → 535

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

COG0666
Location:910 → 1109

ANKYR; Ankyrin repeat [Signal transduction mechanisms]

sd00045
Location:1007 → 1038

ANK; ANK repeat [structural motif]

pfam12796
Location:977 → 1072

Ank_2; Ankyrin repeats (3 copies)

cd20850
Location:163 → 235

C1_DGKiota_rpt1; first protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase iota (DAG kinase iota) and similar proteins

cd20896
Location:244 → 318

C1_DGKiota_rpt2; second protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase iota (DAG kinase iota) and similar proteins
XM_039108371.2 → XP_038964299.1 diacylglycerol kinase iota isoform X8

UniProtKB/TrEMBL

A0A8I6GM87

Conserved Domains (7) summary

smart00045
Location:457 → 614

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:309 → 430

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

COG0666
Location:805 → 1004

ANKYR; Ankyrin repeat [Signal transduction mechanisms]

sd00045
Location:902 → 933

ANK; ANK repeat [structural motif]

pfam12796
Location:872 → 967

Ank_2; Ankyrin repeats (3 copies)

cd20850
Location:163 → 235

C1_DGKiota_rpt1; first protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase iota (DAG kinase iota) and similar proteins

cl00040
Location:246 → 264

C1; protein kinase C conserved region 1 (C1 domain) superfamily
XM_063286710.1 → XP_063142780.1 diacylglycerol kinase iota isoform X9

UniProtKB/TrEMBL

A0A8I6GM87
XM_039108370.2 → XP_038964298.1 diacylglycerol kinase iota isoform X7

UniProtKB/TrEMBL

A0A8I6GM87

Conserved Domains (7) summary

smart00045
Location:458 → 615

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:310 → 431

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

COG0666
Location:806 → 1005

ANKYR; Ankyrin repeat [Signal transduction mechanisms]

sd00045
Location:903 → 934

ANK; ANK repeat [structural motif]

pfam12796
Location:873 → 968

Ank_2; Ankyrin repeats (3 copies)

cd20850
Location:163 → 235

C1_DGKiota_rpt1; first protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase iota (DAG kinase iota) and similar proteins

cl00040
Location:246 → 264

C1; protein kinase C conserved region 1 (C1 domain) superfamily
XM_063286709.1 → XP_063142779.1 diacylglycerol kinase iota isoform X6

UniProtKB/TrEMBL

A0A8I6GM87
XM_017592894.3 → XP_017448383.1 diacylglycerol kinase iota isoform X5

UniProtKB/TrEMBL

A0A8I6GM87
XM_006236333.5 → XP_006236395.1 diacylglycerol kinase iota isoform X4

UniProtKB/TrEMBL

A0A8I5ZPA8, A0A8I6GM87

Related

ENSRNOP00000079887.1, ENSRNOT00000120235.2

Conserved Domains (7) summary

smart00045
Location:521 → 678

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:373 → 494

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

COG1597
Location:369 → 688

LCB5; Diacylglycerol kinase family enzyme [Lipid transport and metabolism, General function prediction only]

cd00204
Location:939 → 1053

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam00130
Location:246 → 305

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam12796
Location:936 → 1031

Ank_2; Ankyrin repeats (3 copies)

sd00045
Location:966 → 997

ANK; ANK repeat [structural motif]
XM_039108369.2 → XP_038964297.1 diacylglycerol kinase iota isoform X3

UniProtKB/TrEMBL

A0A8I6GM87

Related

ENSRNOP00000072665.3, ENSRNOT00000079285.3

Conserved Domains (7) summary

smart00045
Location:562 → 719

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:414 → 535

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

COG0666
Location:889 → 1088

ANKYR; Ankyrin repeat [Signal transduction mechanisms]

sd00045
Location:986 → 1017

ANK; ANK repeat [structural motif]

pfam12796
Location:956 → 1051

Ank_2; Ankyrin repeats (3 copies)

cd20850
Location:163 → 235

C1_DGKiota_rpt1; first protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase iota (DAG kinase iota) and similar proteins

cd20896
Location:244 → 318

C1_DGKiota_rpt2; second protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase iota (DAG kinase iota) and similar proteins
XM_039108372.2 → XP_038964300.1 diacylglycerol kinase iota isoform X10

UniProtKB/TrEMBL

A0A0G2K3J7

Conserved Domains (4) summary

smart00046
Location:414 → 535

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd20850
Location:163 → 235

C1_DGKiota_rpt1; first protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase iota (DAG kinase iota) and similar proteins

cd20896
Location:244 → 318

C1_DGKiota_rpt2; second protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase iota (DAG kinase iota) and similar proteins

cl02440
Location:562 → 624

DAGK_acc; Diacylglycerol kinase accessory domain
XM_039108374.2 → XP_038964302.1 diacylglycerol kinase iota isoform X11

UniProtKB/TrEMBL

A0A0G2K3J7

Conserved Domains (4) summary

smart00046
Location:373 → 494

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd20850
Location:163 → 235

C1_DGKiota_rpt1; first protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase iota (DAG kinase iota) and similar proteins

cd20896
Location:244 → 318

C1_DGKiota_rpt2; second protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase iota (DAG kinase iota) and similar proteins

cl02440
Location:521 → 583

DAGK_acc; Diacylglycerol kinase accessory domain