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Sample GSM7256639

Query DataSets for GSM7256639

Status

Public on Apr 30, 2023

Title

Control ChIP-seq from HCT116 (ENCLB329HYH)

Sample type

SRA

Source name

Homo sapiens HCT116 cell line genetically modified (insertion) using CRISPR inserting O. sativa LOC4335696, genetically modified (insertion) using CRISPR targeting H. sapiens MED14

Organism

Homo sapiens

Characteristics

line: HCT116
biomaterial_type: cell line
biosample encode accession: ENCBS658CIB (SAMN34251794)
Sex: male
health state: colorectal carcinoma
dev stage: adult
age: 48 year
donor_id: ENCDO000ABE
lab: Bradley Bernstein, Broad
source: Erez Aiden
link: ENCBS658CIB at ENCODE; https://www.encodeproject.org/ENCBS658CIB/
link: derived from ENCODE donor ENCDO000ABE; https://www.encodeproject.org/ENCDO000ABE/

Extracted molecule

genomic DNA

Extraction protocol

general protocol: https://www.encodeproject.org/documents/801dd1e1-5e10-4792-b848-b094380d1444/@@download/attachment/Crosslinking%20Cells%20v4.0.pdf
general protocol: https://www.encodeproject.org/documents/ceb000f0-e354-4138-967f-f4314b8c5d98/@@download/attachment/Epigenomics%20KAPA%20Hyper%20kit%20semi%20Manual%20Library%20Construction%20v2.4.pdf

Library strategy

ChIP-Seq

Library source

genomic

Library selection

ChIP

Instrument model

Illumina NextSeq 500

Description

https://www.encodeproject.org/experiments/ENCSR990OHC/
***************
biological replicate number: 1
technical replicate number: 2
description:
experiment encode accession: ENCSR990OHC
assay title: Control ChIP-seq
assembly: GRCh38
encode release date: 2022-07-21
lab: Bradley Bernstein, Broad
library encode accession: ENCLB329HYH
size range: 200-600

Data processing

See GSM*_README.txt supplementary file linked below

Submission date

Apr 28, 2023

Last update date

May 02, 2023

Contact name

ENCODE DCC

E-mail(s)

[email protected]

Organization name

ENCODE DCC

Street address

300 Pasteur Dr

City

Stanford

State/province

ZIP/Postal code

94305-5120

Country

USA

Platform ID

GPL18573

Series (1)

GSE231298

Control ChIP-seq from HCT116 (ENCSR990OHC)

Relations

BioSample

SAMN34251794

SRA

SRX20151090

Supplementary file	Size	Download	File type/resource
GSM7256639_ENCLB329HYH_README.txt.gz	291 b	(ftp)(http)	TXT
SRA Run Selector
Raw data are available in SRA
Processed data provided as supplementary file