LOCUS NM_001106514 3226 bp mRNA linear ROD 17-JUL-2024
DEFINITION Rattus norvegicus minichromosome maintenance 8 homologous
recombination repair factor (Mcm8), transcript variant 2, mRNA.
ACCESSION NM_001106514 XM_001079873 XM_215825
VERSION NM_001106514.1
KEYWORDS RefSeq.
SOURCE Rattus norvegicus (Norway rat)
ORGANISM Rattus norvegicus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Rattus.
REFERENCE 1 (bases 1 to 3226)
AUTHORS Traver,S., Coulombe,P., Peiffer,I., Hutchins,J.R., Kitzmann,M.,
Latreille,D. and Mechali,M.
TITLE MCM9 Is Required for Mammalian DNA Mismatch Repair
JOURNAL Mol Cell 59 (5), 831-839 (2015)
PUBMED 26300262
REFERENCE 2 (bases 1 to 3226)
AUTHORS Lee,K.Y., Im,J.S., Shibata,E., Park,J., Handa,N.,
Kowalczykowski,S.C. and Dutta,A.
TITLE MCM8-9 complex promotes resection of double-strand break ends by
MRE11-RAD50-NBS1 complex
JOURNAL Nat Commun 6, 7744 (2015)
PUBMED 26215093
REMARK Publication Status: Online-Only
REFERENCE 3 (bases 1 to 3226)
AUTHORS Park,J., Long,D.T., Lee,K.Y., Abbas,T., Shibata,E., Negishi,M.,
Luo,Y., Schimenti,J.C., Gambus,A., Walter,J.C. and Dutta,A.
TITLE The MCM8-MCM9 complex promotes RAD51 recruitment at DNA damage
sites to facilitate homologous recombination
JOURNAL Mol Cell Biol 33 (8), 1632-1644 (2013)
PUBMED 23401855
REFERENCE 4 (bases 1 to 3226)
AUTHORS Lutzmann,M., Grey,C., Traver,S., Ganier,O., Maya-Mendoza,A.,
Ranisavljevic,N., Bernex,F., Nishiyama,A., Montel,N., Gavois,E.,
Forichon,L., de Massy,B. and Mechali,M.
TITLE MCM8- and MCM9-deficient mice reveal gametogenesis defects and
genome instability due to impaired homologous recombination
JOURNAL Mol Cell 47 (4), 523-534 (2012)
PUBMED 22771120
REFERENCE 5 (bases 1 to 3226)
AUTHORS Nishimura,K., Ishiai,M., Horikawa,K., Fukagawa,T., Takata,M.,
Takisawa,H. and Kanemaki,M.T.
TITLE Mcm8 and Mcm9 form a complex that functions in homologous
recombination repair induced by DNA interstrand crosslinks
JOURNAL Mol Cell 47 (4), 511-522 (2012)
PUBMED 22771115
COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final
NCBI review. The reference sequence was derived from CH473949.1.
On or before Oct 4, 2007 this sequence version replaced
XM_001079873.1, XM_215825.4.
##Evidence-Data-START##
RNAseq introns :: single sample supports all introns SAMD00132296,
SAMD00132304 [ECO:0000348]
##Evidence-Data-END##
FEATURES Location/Qualifiers
source 1..3226
/organism="Rattus norvegicus"
/mol_type="mRNA"
/db_xref="taxon:10116"
/chromosome="3"
/map="3q36"
gene 1..3226
/gene="Mcm8"
/note="minichromosome maintenance 8 homologous
recombination repair factor"
/db_xref="GeneID:296178"
/db_xref="RGD:1305218"
exon 1..266
/gene="Mcm8"
/inference="alignment:Splign:2.1.0"
exon 267..429
/gene="Mcm8"
/inference="alignment:Splign:2.1.0"
misc_feature 276..278
/gene="Mcm8"
/note="upstream in-frame stop codon"
CDS 291..2783
/gene="Mcm8"
/EC_number="3.6.4.12"
/note="isoform a is encoded by transcript variant 2;
minichromosome maintenance deficient 8; DNA replication
licensing factor MCM8; DNA helicase MCM8; minichromosome
maintenance complex component 8"
/codon_start=1
/product="DNA helicase MCM8 isoform a"
/protein_id="NP_001099984.1"
/db_xref="GeneID:296178"
/db_xref="RGD:1305218"
/translation="MSGAYRGRGFGRGRFQNWKRGRGGGNFSGRWRDRTDLSKAAGNH
ASEQASQPLLQQSTLDQFIPYKGWKLYFSEVYSNNSPLTEKIQAFEKFFTRHIDLYDK
DEIERKGSILVDFKELTKDNEITNLIPDIENALRDAPEKTLACMGLAIHQVLTKDLER
HAAELQAQEGLCNGGGTMVNVPHIYARVYNYEPLTHLKNIRATCYGKYISIRGTVVRV
SNIKPLCTKMAFQCAACGEIQSFPLPDGKYNLPTKCPVPACRGRSFTPLRSSPLTVTM
DWQLIKIQELMSDAQREAGRIPRTIECELVHDLVDSCVPGDTVTVTGIVKVSNSEEGS
RSKNDKCMFLLYIEANSVSNSKGQKAQTAEDGCKHGTLMEFSLKDLYAIQEIQAEENL
LKLIVNSLCPVIFGHELVKAGLMLALFGGSQKYADDKNRIPIRGDPHVLIVGDPGLGK
SQMLQAACNVAPRGVYVCGNTATSSGLTVTLSKDSSSGDFALEAGALVLGDQGICGID
EFDKMGNQHQALLEAMEQQSISLAKAGVVCSLPARTSIIAAANPVGGHYNKARTVSEN
LKMGSALLSRFDLVFILLDTPNEQHDHLLSEHVIAIRAGKQRAVSSATVSRVLSQDSN
TSVLEVVSEKPLSERLKVAPGEKTDPIPHQLLRKYIGYARQYVHPRLSTEAAQALQDF
YLELRKQSQRVGSSPITTRQLESLIRLTEARARLELREEATKEDAEDIIEIMKHSMLG
TYSDEFGNLDFERSQHGSGMSNRSTAKRFISALNSIAERTYNNIFQFHQLRQIAKELN
IQVADFENFIGSLNDQGYLLKKGPKIYQLQTM"
misc_feature 2148..2150
/gene="Mcm8"
/note="Phosphoserine.
/evidence=ECO:0000250|UniProtKB:Q9UJA3; propagated from
UniProtKB/Swiss-Prot (D3ZVK1.1); phosphorylation site"
exon 430..513
/gene="Mcm8"
/inference="alignment:Splign:2.1.0"
exon 514..596
/gene="Mcm8"
/inference="alignment:Splign:2.1.0"
exon 597..746
/gene="Mcm8"
/inference="alignment:Splign:2.1.0"
exon 747..850
/gene="Mcm8"
/inference="alignment:Splign:2.1.0"
exon 851..1049
/gene="Mcm8"
/inference="alignment:Splign:2.1.0"
exon 1050..1135
/gene="Mcm8"
/inference="alignment:Splign:2.1.0"
exon 1136..1287
/gene="Mcm8"
/inference="alignment:Splign:2.1.0"
exon 1288..1483
/gene="Mcm8"
/inference="alignment:Splign:2.1.0"
exon 1484..1514
/gene="Mcm8"
/inference="alignment:Splign:2.1.0"
exon 1515..1655
/gene="Mcm8"
/inference="alignment:Splign:2.1.0"
exon 1656..1797
/gene="Mcm8"
/inference="alignment:Splign:2.1.0"
exon 1798..1993
/gene="Mcm8"
/inference="alignment:Splign:2.1.0"
exon 1994..2213
/gene="Mcm8"
/inference="alignment:Splign:2.1.0"
exon 2214..2423
/gene="Mcm8"
/inference="alignment:Splign:2.1.0"
exon 2424..2500
/gene="Mcm8"
/inference="alignment:Splign:2.1.0"
exon 2501..2690
/gene="Mcm8"
/inference="alignment:Splign:2.1.0"
exon 2691..3226
/gene="Mcm8"
/inference="alignment:Splign:2.1.0"
ORIGIN
1 cttcgagcga ggtcggagtt taccctgtgg tgttgtgccg ggccttcgcc gccctgcgtc
61 tcgcctcggg cgactccgaa gacggaatgt ctgcgtaacc ctgcgtggga ctgcgtaccg
121 cctatggctc tgtggctggc tgggagactc gggggactcc tgaaagtagg acgcgagaaa
181 aacgcggcga gaatgaagtc agggattgcg gggggctttt ctcaggctgc ttccctatgt
241 agttgttgga agtgcctccg ttctcaaagt gctgttgact ctcagaagac atgagtggtg
301 catacagagg cagaggtttt ggacgaggac gattccaaaa ctggaaaaga ggaagaggtg
361 gtgggaactt ctcaggaagg tggagagaca gaactgacct gagtaaagct gcaggaaatc
421 atgcttctga acaagcctca caacctttac ttcaacagtc aacattggat cagtttatac
481 cgtataaggg ctggaagctt tacttctctg aagtttacag taataactct cctcttactg
541 agaagatcca agcatttgag aaatttttca caaggcatat cgacttatat gataaggatg
601 aaatagaaag aaagggaagc atcttggtgg attttaaaga actgacaaaa gacaatgaaa
661 taactaactt gataccagat atagaaaatg cactaagaga tgcccctgag aaaacactgg
721 catgtatggg actggcaata catcaggtat taactaagga ccttgaaagg cacgcagctg
781 aattacaagc tcaagagggg ttgtgtaatg gtggaggaac catggtaaat gtgccacata
841 tttatgcgag agtgtacaac tatgagccct taacacacct caagaacatc cgagcaactt
901 gctatgggaa gtacatctct ataagaggga cagtggttcg agtcagtaac ataaagcctc
961 tgtgcaccaa aatggctttt cagtgtgctg catgtggaga gattcagagc tttcctcttc
1021 cggatggaaa atacaatctt ccaacaaagt gtcctgtgcc tgcgtgtcga gggaggtcat
1081 ttacgccatt gcgcagttct cctctcaccg tcacaatgga ctggcagttg atcaaaatcc
1141 aggagctgat gtctgatgca cagagagaag ctggtcggat ccctcgaacc atagaatgtg
1201 aacttgttca tgatcttgta gatagctgtg tcccaggaga tacggtgact gttactggaa
1261 ttgtcaaagt ttcaaatagt gaagaaggtt ctcgatctaa gaatgataag tgcatgttcc
1321 ttttgtacat tgaagcaaac tctgttagca acagcaaagg acagaaagcc cagactgcag
1381 aggatggttg caagcatgga acactgatgg agttctccct taaagacctg tatgccatcc
1441 aagagatcca ggctgaagag aacctgctca agctcattgt caactcactt tgtcctgtca
1501 tttttggtca tgaacttgtt aaagcaggac tgatgttagc gctatttggc ggtagccaga
1561 agtacgcaga tgacaaaaac aggattccta ttcgaggaga cccacacgtc ctgattgttg
1621 gagatccagg cttggggaag agtcagatgc tgcaggcagc atgcaacgtg gcgccacgtg
1681 gtgtgtatgt ttgtggaaat acggccacca gctctggtct gacggttact ctttcaaaag
1741 acagttcctc aggagacttc gctttggaag ctggtgccct ggtacttggt gaccaaggca
1801 tttgtggaat agatgaattt gataaaatgg ggaatcagca tcaagccttg ttagaagcca
1861 tggaacagca gagcattagc cttgctaagg ccggtgtggt ttgcagcctc cctgcaagaa
1921 cctccattat tgctgcggca aatccagtcg gaggacacta caataaagcg aggacagttt
1981 ctgagaattt aaagatgggg agtgccctgc tgtccaggtt cgatttggtc tttatcttgt
2041 tagatacacc aaatgaacag cacgaccact tactttctga acatgtgatc gcaataagag
2101 ctgggaagca gagagcggtt agcagtgcca cagtctctcg tgtgctcagt caagactcaa
2161 atacttctgt acttgaagtg gtttccgaga aaccgttatc agaaagacta aaggtggctc
2221 ctggagaaaa gacagatccg attccacatc agctgctgag gaagtacata ggctatgcac
2281 ggcagtatgt ccatccaagg ttatccacgg aagctgctca ggctcttcag gatttctacc
2341 tggagctgcg caagcagagc cagcgcgtgg gcagctcgcc catcaccact cggcagctgg
2401 aatctttgat ccgtctgacg gaggcacgag caaggttaga attgagagag gaagcaacta
2461 aagaagacgc tgaagatata attgaaatta tgaagcatag catgctagga acttactcag
2521 atgaatttgg aaacctggac tttgagcgat cccagcatgg ctctggaatg agcaacaggt
2581 caacagctaa aagatttatt tctgctctca acagcattgc tgaaagaacg tataacaaca
2641 tatttcaatt tcatcaactt cgtcagattg ctaaagagct aaacattcag gttgctgact
2701 ttgaaaactt cattggatca ctgaatgacc agggttatct cctgaaaaaa ggcccaaaga
2761 tttatcaact tcaaactatg tgaaagactt tcctatgtca gggcctcctg tagttcaaaa
2821 aatttcagaa tcatcttaaa tataaaactg aaaataaaag ttctgaaaaa tgacacaact
2881 cataacagaa aaaaaggtat atccataatt taccagaaga atacgaagag taaattattt
2941 ccactatttt atctaaataa cataagcatt atttatgctt ttggcttata tttttattag
3001 tgctttttag ttttgtatat cagatgtcta gcaggcattc agataatata taaattagta
3061 tgtgtttaag ttttatttaa aacattaaac tttgaatctc acaaattgtt aaggtcacaa
3121 aaactcttca tttgtttcta actttatggt tatgggcagt gggtctgact tggcttctta
3181 tttgaaagtg gtggaaactt attgacttaa ttagaagtct tttctg
//